Planarian EST Database


Dr_sW_022_K12

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_022_K12
         (595 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q99437|VATO_HUMAN  Vacuolar ATP synthase 21 kDa proteolip...   218   9e-57
sp|Q91V37|VATO_MOUSE  Vacuolar ATP synthase 21 kDa proteolip...   217   2e-56
sp|P23968|VATO_YEAST  Vacuolar ATP synthase 22 kDa proteolip...   167   2e-41
sp|O14046|VATO_SCHPO  Probable vacuolar ATP synthase 20 kDa ...   164   2e-40
sp|P59229|VATL4_ARATH  Vacuolar ATP synthase 16 kDa proteoli...    75   9e-14
sp|P68161|VATL_MESCR  Vacuolar ATP synthase 16 kDa proteolip...    75   1e-13
sp|P59228|VATL2_ARATH  Vacuolar ATP synthase 16 kDa proteoli...    75   1e-13
sp|P59227|VATL1_ARATH  Vacuolar ATP synthase 16 kDa proteoli...    75   1e-13
sp|Q43434|VATL_GOSHI  Vacuolar ATP synthase 16 kDa proteolip...    75   1e-13
sp|O24011|VATL_LYCES  Vacuolar ATP synthase 16 kDa proteolip...    75   1e-13
>sp|Q99437|VATO_HUMAN Vacuolar ATP synthase 21 kDa proteolipid subunit (HATPL)
          Length = 205

 Score =  218 bits (555), Expect = 9e-57
 Identities = 118/176 (67%), Positives = 132/176 (75%), Gaps = 3/176 (1%)
 Frame = +3

Query: 3   RFDIGWFLQETSPYLWANIGIGLCISLSVLGAALGIYTTGSSILGAGVKTPRIRTKNLVS 182
           RFD+ WFL ETSP++W+N+GIGL ISLSV+GAA GIY TGSSI+G GVK PRI+TKNLVS
Sbjct: 34  RFDVAWFLTETSPFMWSNLGIGLAISLSVVGAAWGIYITGSSIIGGGVKAPRIKTKNLVS 93

Query: 183 IIFCEAVAIYGIIMAIVISQNIKSF---DSKIASFRVLQINHEGGFRMFGSGLTVGLSNL 353
           IIFCEAVAIYGIIMAIVIS   + F   D K    R    N+  G+ MFG+GLTVGLSNL
Sbjct: 94  IIFCEAVAIYGIIMAIVISNMAEPFSATDPKAIGHR----NYHAGYSMFGAGLTVGLSNL 149

Query: 354 XXXXXXXXXXXXAALADSANASLFVKILIVEIFGSAIGLFGVIVGILQVAGTKMGD 521
                       AALAD+ N SLFVKILIVEIFGSAIGLFGVIV ILQ +  KMGD
Sbjct: 150 FCGVCVGIVGSGAALADAQNPSLFVKILIVEIFGSAIGLFGVIVAILQTSRVKMGD 205
>sp|Q91V37|VATO_MOUSE Vacuolar ATP synthase 21 kDa proteolipid subunit (23-kDa subunit of
           V-ATPase)
          Length = 205

 Score =  217 bits (552), Expect = 2e-56
 Identities = 116/173 (67%), Positives = 131/173 (75%)
 Frame = +3

Query: 3   RFDIGWFLQETSPYLWANIGIGLCISLSVLGAALGIYTTGSSILGAGVKTPRIRTKNLVS 182
           RFD+ WFL ETSP++W+N+GIGL ISLSV+GAA GIY TGSSI+G GVK PRI+TKNLVS
Sbjct: 34  RFDVAWFLTETSPFMWSNLGIGLAISLSVVGAAWGIYITGSSIIGGGVKAPRIKTKNLVS 93

Query: 183 IIFCEAVAIYGIIMAIVISQNIKSFDSKIASFRVLQINHEGGFRMFGSGLTVGLSNLXXX 362
           IIFCEAVAIYGIIMAIVIS   + F S      +   N+  G+ MFG+GLTVGLSNL   
Sbjct: 94  IIFCEAVAIYGIIMAIVISNMAEPF-SATEPKAIGHRNYHAGYSMFGAGLTVGLSNLFCG 152

Query: 363 XXXXXXXXXAALADSANASLFVKILIVEIFGSAIGLFGVIVGILQVAGTKMGD 521
                    AALAD+ N SLFVKILIVEIFGSAIGLFGVIV ILQ +  KMGD
Sbjct: 153 VCVGIVGSGAALADAQNPSLFVKILIVEIFGSAIGLFGVIVAILQTSRVKMGD 205
>sp|P23968|VATO_YEAST Vacuolar ATP synthase 22 kDa proteolipid subunit
          Length = 213

 Score =  167 bits (423), Expect = 2e-41
 Identities = 86/162 (53%), Positives = 115/162 (70%)
 Frame = +3

Query: 9   DIGWFLQETSPYLWANIGIGLCISLSVLGAALGIYTTGSSILGAGVKTPRIRTKNLVSII 188
           + G FL  TSPY+WAN+GI LC+ LSV+GAA GI+ TGSS++GAGV+ PRI TKNL+SII
Sbjct: 46  NFGKFLLRTSPYMWANLGIALCVGLSVVGAAWGIFITGSSMIGAGVRAPRITTKNLISII 105

Query: 189 FCEAVAIYGIIMAIVISQNIKSFDSKIASFRVLQINHEGGFRMFGSGLTVGLSNLXXXXX 368
           FCE VAIYG+I+AIV S  +       A     + N   G+ +F +G+TVG SNL     
Sbjct: 106 FCEVVAIYGLIIAIVFSSKL---TVATAENMYSKSNLYTGYSLFWAGITVGASNLICGIA 162

Query: 369 XXXXXXXAALADSANASLFVKILIVEIFGSAIGLFGVIVGIL 494
                  AA++D+A+++LFVKIL++EIFGS +GL G+IVG+L
Sbjct: 163 VGITGATAAISDAADSALFVKILVIEIFGSILGLLGLIVGLL 204
>sp|O14046|VATO_SCHPO Probable vacuolar ATP synthase 20 kDa proteolipid subunit
          Length = 199

 Score =  164 bits (415), Expect = 2e-40
 Identities = 87/163 (53%), Positives = 111/163 (68%)
 Frame = +3

Query: 6   FDIGWFLQETSPYLWANIGIGLCISLSVLGAALGIYTTGSSILGAGVKTPRIRTKNLVSI 185
           FD G FL +TSPY W  +GI  C++  ++GAA GI+  G+SILG  VK PRI+TKNL+SI
Sbjct: 31  FDFGSFLLDTSPYTWGLLGIASCVAFGIIGAAWGIFICGTSILGGAVKAPRIKTKNLISI 90

Query: 186 IFCEAVAIYGIIMAIVISQNIKSFDSKIASFRVLQINHEGGFRMFGSGLTVGLSNLXXXX 365
           IFCE VAIY +I+AIV S  I   D   A F   + ++  GF +F  G+TVGL NL    
Sbjct: 91  IFCEVVAIYSLIIAIVFSAKIN--DINPAGF-YTKSHYYTGFALFWGGITVGLCNLICGV 147

Query: 366 XXXXXXXXAALADSANASLFVKILIVEIFGSAIGLFGVIVGIL 494
                   AALAD+ +ASLFVK+L+VEIFGS +GLFG+IVG+L
Sbjct: 148 CVGITGSSAALADAQDASLFVKVLVVEIFGSVLGLFGLIVGLL 190

 Score = 34.3 bits (77), Expect = 0.24
 Identities = 22/70 (31%), Positives = 34/70 (48%)
 Frame = +3

Query: 48  WANIGIGLCISLSVLGAALGIYTTGSSILGAGVKTPRIRTKNLVSIIFCEAVAIYGIIMA 227
           W  I +GLC    + G  +GI  TGSS   A  +   +  K LV  IF   + ++G+I+ 
Sbjct: 133 WGGITVGLCNL--ICGVCVGI--TGSSAALADAQDASLFVKVLVVEIFGSVLGLFGLIVG 188

Query: 228 IVISQNIKSF 257
           ++I      F
Sbjct: 189 LLIGGKASDF 198
>sp|P59229|VATL4_ARATH Vacuolar ATP synthase 16 kDa proteolipid subunit 4 (V-ATPase 16 kDa
           proteolipid subunit 4)
          Length = 166

 Score = 75.5 bits (184), Expect = 9e-14
 Identities = 46/160 (28%), Positives = 80/160 (50%)
 Frame = +3

Query: 15  GWFLQETSPYLWANIGIGLCISLSVLGAALGIYTTGSSILGAGVKTPRIRTKNLVSIIFC 194
           G+   ET+P+ +  +G    +  S +GAA G   +G  +   GV  P +  K++V ++  
Sbjct: 5   GFSGDETAPF-FGFLGAAAALVFSCMGAAYGTAKSGVGVASMGVMRPELVMKSIVPVVMA 63

Query: 195 EAVAIYGIIMAIVISQNIKSFDSKIASFRVLQINHEGGFRMFGSGLTVGLSNLXXXXXXX 374
             + IYG+I+A++IS  I   + K  S+ +       G+    SGL  GL+ L       
Sbjct: 64  GVLGIYGLIIAVIISTGI---NPKAKSYYLFD-----GYAHLSSGLACGLAGLSAGMAIG 115

Query: 375 XXXXXAALADSANASLFVKILIVEIFGSAIGLFGVIVGIL 494
                   A++    LFV ++++ IF  A+ L+G+IVGI+
Sbjct: 116 IVGDAGVRANAQQPKLFVGMILILIFAEALALYGLIVGII 155

 Score = 43.1 bits (100), Expect = 5e-04
 Identities = 20/64 (31%), Positives = 39/64 (60%)
 Frame = +3

Query: 48  WANIGIGLCISLSVLGAALGIYTTGSSILGAGVKTPRIRTKNLVSIIFCEAVAIYGIIMA 227
           +A++  GL   L+ L A + I   G + + A  + P++    ++ +IF EA+A+YG+I+ 
Sbjct: 94  YAHLSSGLACGLAGLSAGMAIGIVGDAGVRANAQQPKLFVGMILILIFAEALALYGLIVG 153

Query: 228 IVIS 239
           I++S
Sbjct: 154 IILS 157
>sp|P68161|VATL_MESCR Vacuolar ATP synthase 16 kDa proteolipid subunit
 sp|P68162|VATL_BETVU Vacuolar ATP synthase 16 kDa proteolipid subunit
          Length = 165

 Score = 75.1 bits (183), Expect = 1e-13
 Identities = 45/155 (29%), Positives = 78/155 (50%)
 Frame = +3

Query: 30  ETSPYLWANIGIGLCISLSVLGAALGIYTTGSSILGAGVKTPRIRTKNLVSIIFCEAVAI 209
           ET+P+ +  +G    +  S +GAA G   +G  +   GV  P +  K++V ++    + I
Sbjct: 9   ETAPF-FGFLGAAAALVFSCMGAAYGTAKSGVGVASMGVMRPELVMKSIVPVVMAGVLGI 67

Query: 210 YGIIMAIVISQNIKSFDSKIASFRVLQINHEGGFRMFGSGLTVGLSNLXXXXXXXXXXXX 389
           YG+I+A++IS  I   + K  S+ +       G+    SGL  GL+ L            
Sbjct: 68  YGLIIAVIISTGI---NPKAKSYYLFD-----GYAHLSSGLACGLAGLSAGMAIGIVGDA 119

Query: 390 AALADSANASLFVKILIVEIFGSAIGLFGVIVGIL 494
              A++    LFV ++++ IF  A+ L+G+IVGI+
Sbjct: 120 GVRANAQQPKLFVGMILILIFAEALALYGLIVGII 154

 Score = 43.1 bits (100), Expect = 5e-04
 Identities = 20/64 (31%), Positives = 39/64 (60%)
 Frame = +3

Query: 48  WANIGIGLCISLSVLGAALGIYTTGSSILGAGVKTPRIRTKNLVSIIFCEAVAIYGIIMA 227
           +A++  GL   L+ L A + I   G + + A  + P++    ++ +IF EA+A+YG+I+ 
Sbjct: 93  YAHLSSGLACGLAGLSAGMAIGIVGDAGVRANAQQPKLFVGMILILIFAEALALYGLIVG 152

Query: 228 IVIS 239
           I++S
Sbjct: 153 IILS 156
>sp|P59228|VATL2_ARATH Vacuolar ATP synthase 16 kDa proteolipid subunit 2 (V-ATPase 16 kDa
           proteolipid subunit 2)
          Length = 165

 Score = 75.1 bits (183), Expect = 1e-13
 Identities = 45/155 (29%), Positives = 78/155 (50%)
 Frame = +3

Query: 30  ETSPYLWANIGIGLCISLSVLGAALGIYTTGSSILGAGVKTPRIRTKNLVSIIFCEAVAI 209
           ET+P+ +  +G    +  S +GAA G   +G  +   GV  P +  K++V ++    + I
Sbjct: 9   ETAPF-FGFLGAAAALVFSCMGAAYGTAKSGVGVASMGVMRPELVMKSIVPVVMAGVLGI 67

Query: 210 YGIIMAIVISQNIKSFDSKIASFRVLQINHEGGFRMFGSGLTVGLSNLXXXXXXXXXXXX 389
           YG+I+A++IS  I   + K  S+ +       G+    SGL  GL+ L            
Sbjct: 68  YGLIIAVIISTGI---NPKAKSYYLFD-----GYAHLSSGLACGLAGLSAGMAIGIVGDA 119

Query: 390 AALADSANASLFVKILIVEIFGSAIGLFGVIVGIL 494
              A++    LFV ++++ IF  A+ L+G+IVGI+
Sbjct: 120 GVRANAQQPKLFVGMILILIFAEALALYGLIVGII 154

 Score = 43.1 bits (100), Expect = 5e-04
 Identities = 20/64 (31%), Positives = 39/64 (60%)
 Frame = +3

Query: 48  WANIGIGLCISLSVLGAALGIYTTGSSILGAGVKTPRIRTKNLVSIIFCEAVAIYGIIMA 227
           +A++  GL   L+ L A + I   G + + A  + P++    ++ +IF EA+A+YG+I+ 
Sbjct: 93  YAHLSSGLACGLAGLSAGMAIGIVGDAGVRANAQQPKLFVGMILILIFAEALALYGLIVG 152

Query: 228 IVIS 239
           I++S
Sbjct: 153 IILS 156
>sp|P59227|VATL1_ARATH Vacuolar ATP synthase 16 kDa proteolipid subunit 1/3/5 (V-ATPase 16
           kDa proteolipid subunit 1/3/5)
          Length = 164

 Score = 75.1 bits (183), Expect = 1e-13
 Identities = 45/155 (29%), Positives = 78/155 (50%)
 Frame = +3

Query: 30  ETSPYLWANIGIGLCISLSVLGAALGIYTTGSSILGAGVKTPRIRTKNLVSIIFCEAVAI 209
           ET+P+ +  +G    +  S +GAA G   +G  +   GV  P +  K++V ++    + I
Sbjct: 8   ETAPF-FGFLGAAAALVFSCMGAAYGTAKSGVGVASMGVMRPELVMKSIVPVVMAGVLGI 66

Query: 210 YGIIMAIVISQNIKSFDSKIASFRVLQINHEGGFRMFGSGLTVGLSNLXXXXXXXXXXXX 389
           YG+I+A++IS  I   + K  S+ +       G+    SGL  GL+ L            
Sbjct: 67  YGLIIAVIISTGI---NPKAKSYYLFD-----GYAHLSSGLACGLAGLSAGMAIGIVGDA 118

Query: 390 AALADSANASLFVKILIVEIFGSAIGLFGVIVGIL 494
              A++    LFV ++++ IF  A+ L+G+IVGI+
Sbjct: 119 GVRANAQQPKLFVGMILILIFAEALALYGLIVGII 153

 Score = 43.1 bits (100), Expect = 5e-04
 Identities = 20/64 (31%), Positives = 39/64 (60%)
 Frame = +3

Query: 48  WANIGIGLCISLSVLGAALGIYTTGSSILGAGVKTPRIRTKNLVSIIFCEAVAIYGIIMA 227
           +A++  GL   L+ L A + I   G + + A  + P++    ++ +IF EA+A+YG+I+ 
Sbjct: 92  YAHLSSGLACGLAGLSAGMAIGIVGDAGVRANAQQPKLFVGMILILIFAEALALYGLIVG 151

Query: 228 IVIS 239
           I++S
Sbjct: 152 IILS 155
>sp|Q43434|VATL_GOSHI Vacuolar ATP synthase 16 kDa proteolipid subunit
          Length = 165

 Score = 75.1 bits (183), Expect = 1e-13
 Identities = 45/155 (29%), Positives = 78/155 (50%)
 Frame = +3

Query: 30  ETSPYLWANIGIGLCISLSVLGAALGIYTTGSSILGAGVKTPRIRTKNLVSIIFCEAVAI 209
           ET+P+ +  +G    +  S +GAA G   +G  +   GV  P +  K++V ++    + I
Sbjct: 9   ETAPF-FGFLGAAAALVFSCMGAAYGTAKSGVGVASMGVMRPELVMKSIVPVVMAGVLGI 67

Query: 210 YGIIMAIVISQNIKSFDSKIASFRVLQINHEGGFRMFGSGLTVGLSNLXXXXXXXXXXXX 389
           YG+I+A++IS  I   + K  S+ +       G+    SGL  GL+ L            
Sbjct: 68  YGLIIAVIISTGI---NPKAKSYYLFD-----GYAHLSSGLACGLAGLSAGMAIGIVGDA 119

Query: 390 AALADSANASLFVKILIVEIFGSAIGLFGVIVGIL 494
              A++    LFV ++++ IF  A+ L+G+IVGI+
Sbjct: 120 GVRANAQQPKLFVGMILILIFAEALALYGLIVGII 154

 Score = 43.1 bits (100), Expect = 5e-04
 Identities = 20/64 (31%), Positives = 39/64 (60%)
 Frame = +3

Query: 48  WANIGIGLCISLSVLGAALGIYTTGSSILGAGVKTPRIRTKNLVSIIFCEAVAIYGIIMA 227
           +A++  GL   L+ L A + I   G + + A  + P++    ++ +IF EA+A+YG+I+ 
Sbjct: 93  YAHLSSGLACGLAGLSAGMAIGIVGDAGVRANAQQPKLFVGMILILIFAEALALYGLIVG 152

Query: 228 IVIS 239
           I++S
Sbjct: 153 IILS 156
>sp|O24011|VATL_LYCES Vacuolar ATP synthase 16 kDa proteolipid subunit
          Length = 164

 Score = 75.1 bits (183), Expect = 1e-13
 Identities = 45/155 (29%), Positives = 78/155 (50%)
 Frame = +3

Query: 30  ETSPYLWANIGIGLCISLSVLGAALGIYTTGSSILGAGVKTPRIRTKNLVSIIFCEAVAI 209
           ET+P+ +  +G    +  S +GAA G   +G  +   GV  P +  K++V ++    + I
Sbjct: 8   ETAPF-FGFLGAAAALVFSCMGAAYGTAKSGVGVASMGVMRPELVMKSIVPVVMAGVLGI 66

Query: 210 YGIIMAIVISQNIKSFDSKIASFRVLQINHEGGFRMFGSGLTVGLSNLXXXXXXXXXXXX 389
           YG+I+A++IS  I   + K  S+ +       G+    SGL  GL+ L            
Sbjct: 67  YGLIIAVIISTGI---NPKTKSYYLFD-----GYAHLSSGLACGLAGLSAGMAIGIVGDA 118

Query: 390 AALADSANASLFVKILIVEIFGSAIGLFGVIVGIL 494
              A++    LFV ++++ IF  A+ L+G+IVGI+
Sbjct: 119 GVRANAQQPKLFVGMILILIFAEALALYGLIVGII 153

 Score = 43.1 bits (100), Expect = 5e-04
 Identities = 20/64 (31%), Positives = 39/64 (60%)
 Frame = +3

Query: 48  WANIGIGLCISLSVLGAALGIYTTGSSILGAGVKTPRIRTKNLVSIIFCEAVAIYGIIMA 227
           +A++  GL   L+ L A + I   G + + A  + P++    ++ +IF EA+A+YG+I+ 
Sbjct: 92  YAHLSSGLACGLAGLSAGMAIGIVGDAGVRANAQQPKLFVGMILILIFAEALALYGLIVG 151

Query: 228 IVIS 239
           I++S
Sbjct: 152 IILS 155
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 64,746,048
Number of Sequences: 369166
Number of extensions: 1206440
Number of successful extensions: 3255
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3082
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3195
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4504118060
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)