Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_022_K05
(679 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P26624|GST28_SCHJA Glutathione S-transferase 28 kDa (GST... 130 3e-30
sp|P30114|GST28_SCHHA Glutathione S-transferase 28 kDa (GST... 117 2e-26
sp|P09792|GST28_SCHMA Glutathione S-transferase 28 kDa (GST... 117 2e-26
sp|P30113|GST28_SCHBO Glutathione S-transferase 28 kDa (GST... 114 2e-25
sp|O73888|PTGD2_CHICK Glutathione-requiring prostaglandin D... 100 3e-21
sp|O16116|GST3_CAEEL Glutathione S-transferase 3 (GST class... 100 3e-21
sp|P46088|GST_OMMSL Glutathione S-transferase (GST class-si... 97 5e-20
sp|P46436|GST1_ASCSU Glutathione S-transferase 1 (GST class... 96 9e-20
sp|P46429|GST2_MANSE Glutathione S-transferase 2 (GST class... 94 4e-19
sp|O35543|PTGD2_RAT Glutathione-requiring prostaglandin D s... 93 6e-19
>sp|P26624|GST28_SCHJA Glutathione S-transferase 28 kDa (GST 28) (SJ28 antigen) (GST
class-mu)
Length = 206
Score = 130 bits (327), Expect = 3e-30
Identities = 71/170 (41%), Positives = 106/170 (62%), Gaps = 7/170 (4%)
Frame = +1
Query: 19 VKVTYFNARGRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEGKK--- 189
VK+ YFN RGRAE IR++L A+ VEFED R+ + W +KPT P G+LP+V+ K+
Sbjct: 1 VKLIYFNGRGRAEPIRMILVAAGVEFEDERIEFQDWPKIKPTIPGGRLPIVKITDKRGDV 60
Query: 190 --LTQSMAIARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKIYFAPEEKKEDLRT 363
+++S+AIAR IARK+ MG+ D EYY+IE+ IGQ+ DV +K P E+KE +
Sbjct: 61 KTMSESLAIARFIARKHNMMGDTDDEYYIIEKMIGQVEDVESEYHKTLIKPPEEKEKISK 120
Query: 364 EFVTTSGRDNLKSLEGFIKES--GFFVGEKITLAELFFVVLTDYLEKLPK 507
E + L+++ +KES VG+K+TLA++ + D++ L K
Sbjct: 121 EILNGKVPILLQAICETLKESTGNLTVGDKVTLADVVLIASIDHITDLDK 170
>sp|P30114|GST28_SCHHA Glutathione S-transferase 28 kDa (GST 28) (GST class-mu)
Length = 211
Score = 117 bits (294), Expect = 2e-26
Identities = 66/170 (38%), Positives = 101/170 (59%), Gaps = 7/170 (4%)
Frame = +1
Query: 19 VKVTYFNARGRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEG----- 183
+KV YFN RGRAE IR+ L A+ V +ED R++ + W +KPT P G+LP V+
Sbjct: 6 IKVIYFNGRGRAESIRMTLVAAGVNYEDERISFQDWPKIKPTIPGGRLPAVKITDNHGHV 65
Query: 184 KKLTQSMAIARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKIYFAPEEKKEDLRT 363
K + +S+AIAR +A+K+ MGE D+EYY +E+ IGQ+ D+ +K PEE+K+ +
Sbjct: 66 KWMLESLAIARYMAKKHHMMGETDEEYYNVEKLIGQVEDLEHEYHKTLMKPEEEKQKITK 125
Query: 364 EFVTTSGRDNLKSLEGFIKES--GFFVGEKITLAELFFVVLTDYLEKLPK 507
E + L + +K S VG+K+TLA+L + + D++ L K
Sbjct: 126 EILNGKVPVLLDIICESLKASTGKLAVGDKVTLADLVLIAVIDHVTDLDK 175
>sp|P09792|GST28_SCHMA Glutathione S-transferase 28 kDa (GST 28) (SM28 antigen)
(Protective 28 kDa antigen) (GST class-mu)
Length = 211
Score = 117 bits (294), Expect = 2e-26
Identities = 66/170 (38%), Positives = 101/170 (59%), Gaps = 7/170 (4%)
Frame = +1
Query: 19 VKVTYFNARGRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEG----- 183
+KV YF+ RGRAE IR+ L A+ V++ED R++ + W +KPT P G+LP V+
Sbjct: 6 IKVIYFDGRGRAESIRMTLVAAGVDYEDERISFQDWPKIKPTIPGGRLPAVKVTDDHGHV 65
Query: 184 KKLTQSMAIARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKIYFAPEEKKEDLRT 363
K + +S+AIAR +A+K+ MGE D+EYY +E+ IGQ DV +K P+E+KE +
Sbjct: 66 KWMLESLAIARYMAKKHHMMGETDEEYYSVEKLIGQAEDVEHEYHKTLMKPQEEKEKITK 125
Query: 364 EFVTTSGRDNLKSLEGFIKES--GFFVGEKITLAELFFVVLTDYLEKLPK 507
E + L + +K S VG+K+TLA+L + + D++ L K
Sbjct: 126 EILNGKVPVLLNMICESLKGSTGKLAVGDKVTLADLVLIAVIDHVTDLDK 175
>sp|P30113|GST28_SCHBO Glutathione S-transferase 28 kDa (GST 28) (GST class-mu)
Length = 211
Score = 114 bits (285), Expect = 2e-25
Identities = 65/170 (38%), Positives = 99/170 (58%), Gaps = 7/170 (4%)
Frame = +1
Query: 19 VKVTYFNARGRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEG----- 183
+KV YFN RGRAE IR+ L A+ V +ED R++ + W +KPT P G+LP V+
Sbjct: 6 IKVIYFNGRGRAESIRMTLVAAGVNYEDERISFQDWPKIKPTIPGGRLPAVKITDNHGHV 65
Query: 184 KKLTQSMAIARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKIYFAPEEKKEDLRT 363
K + +S+AIAR +A+K+ MG ++EYY +E+ IGQ D+ YK PEE+K+ +
Sbjct: 66 KWMVESLAIARYMAKKHHMMGGTEEEYYNVEKLIGQAEDLEHEYYKTLMKPEEEKQKIIK 125
Query: 364 EFVTTSGRDNLKSLEGFIKES--GFFVGEKITLAELFFVVLTDYLEKLPK 507
E + L + +K S VG+K+TLA+L + + D++ L K
Sbjct: 126 EILNGKVPVLLDIICESLKASTGKLAVGDKVTLADLVLIAVIDHVTDLDK 175
>sp|O73888|PTGD2_CHICK Glutathione-requiring prostaglandin D synthase
(Glutathione-dependent PGD synthetase) (Prostaglandin-H2
D-isomerase) (Hematopoietic prostaglandin D synthase)
(H-PGDS)
Length = 199
Score = 100 bits (250), Expect = 3e-21
Identities = 58/175 (33%), Positives = 97/175 (55%), Gaps = 12/175 (6%)
Frame = +1
Query: 22 KVTYFNARGRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEGKKLTQS 201
K+TYFN RGRAEI R + + +++ED RL W +KPT P GK+P++E +G + QS
Sbjct: 5 KLTYFNLRGRAEICRYLFAYAGIKYEDHRLEGADWPKIKPTIPFGKVPILEVDGVIIHQS 64
Query: 202 MAIARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKIYFAPEEKKEDLR----TEF 369
+AIAR +AR+ G G+ E L + + + D + + F EK +D++ +
Sbjct: 65 LAIARYLARESGLAGQTPVEQALADAIVDTIDD-----FMMLFPWAEKNQDVKEKAFNDI 119
Query: 370 VTTSGRDNLKSLEGFIKESGFFVGEKITLAELFFVVLT--------DYLEKLPKL 510
+T + LK L+ F+ + +FVG+ +T A+ ++ V + D +K P+L
Sbjct: 120 LTNKAPELLKDLDTFLGDKKWFVGKSVTWADFYWDVCSTTLLSYKADLADKYPRL 174
>sp|O16116|GST3_CAEEL Glutathione S-transferase 3 (GST class-sigma) (CeGST3)
Length = 207
Score = 100 bits (249), Expect = 3e-21
Identities = 59/167 (35%), Positives = 98/167 (58%), Gaps = 7/167 (4%)
Frame = +1
Query: 22 KVTYFNARGRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEGKKLTQS 201
K+TYFNARG AEI R + + VEFED R+ +E++ +KPT P+G++P++ +G + +QS
Sbjct: 5 KLTYFNARGLAEISRQLFHMAGVEFEDERINEEKFSQLKPTFPSGQVPILCIDGAQFSQS 64
Query: 202 MAIARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKIYFA-----PEEKKEDLRTE 366
AIAR +ARK+GF+G+ +E + + D +E K A EE +++R E
Sbjct: 65 TAIARYLARKFGFVGQTAEEELQADEVVDTFKDFIESFRKFVIAVLSGESEEILKNIREE 124
Query: 367 FVTTSGRDNLKSLEGFIKE--SGFFVGEKITLAELFFVVLTDYLEKL 501
+ + + L+ +++ SG+ VG ++T A+L V+ D L L
Sbjct: 125 VIKPAVKTYTAYLKAILEKSSSGYLVGNELTWADL---VIADNLTTL 168
>sp|P46088|GST_OMMSL Glutathione S-transferase (GST class-sigma)
Length = 203
Score = 96.7 bits (239), Expect = 5e-20
Identities = 61/171 (35%), Positives = 92/171 (53%), Gaps = 5/171 (2%)
Frame = +1
Query: 31 YFNARGRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEGKKLTQSMAI 210
YF GRAE+ R VL A EF D + W ++K T + +PV++ +G K++QSM I
Sbjct: 8 YFPLMGRAELCRFVLAAHGEEFTDRVVEMADWPNLKATMYSNAMPVLDIDGTKMSQSMCI 67
Query: 211 ARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKIYFAPEEKKEDLRTEFVTTSGRD 390
AR +AR++G G+ E Y ++ + D+ + KI FAPE KE ++ + + R
Sbjct: 68 ARHLAREFGLDGKTSLEKYRVDEITETLQDIFNDVVKIKFAPEAAKEAVQQNYEKSCKR- 126
Query: 391 NLKSLEGFIKES----GFFVGEKITLAELF-FVVLTDYLEKLPKLYDDFSK 528
LEG + + GFFVG +TLA+L +V L L+ P+L D K
Sbjct: 127 LAPFLEGLLVSNGGGDGFFVGNSMTLADLHCYVALEVPLKHTPELLKDCPK 177
>sp|P46436|GST1_ASCSU Glutathione S-transferase 1 (GST class-sigma)
Length = 206
Score = 95.9 bits (237), Expect = 9e-20
Identities = 62/173 (35%), Positives = 94/173 (54%), Gaps = 6/173 (3%)
Frame = +1
Query: 22 KVTYFNARGRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEGKKLTQS 201
K+TYF+ RG E RL+ + V+FED RL +E W +KP TP G+LP++E +G+ L QS
Sbjct: 5 KLTYFDIRGLGEGARLIFHQAGVKFEDNRLKREDWPALKPKTPFGQLPLLEVDGEVLAQS 64
Query: 202 MAIARAIARKYGFMGEDDKEYYLIERAIGQMVDV---LEGMYKIYFAPEEKKEDLRTEFV 372
AI R + R++G G+ E ++ Q D L +++ EE ++ + V
Sbjct: 65 AAIYRYLGRQFGLAGKTPMEEAQVDSIFDQFKDFMAELRPCFRVLAGFEEGDKEKVLKEV 124
Query: 373 TTSGRD-NLKSLEGFIKESG--FFVGEKITLAELFFVVLTDYLEKLPKLYDDF 522
RD +L LE F+ +SG + VG+ +T A+L V+TD L L DF
Sbjct: 125 AVPARDKHLPLLEKFLAKSGSEYMVGKSVTWADL---VITDSLASWESLIPDF 174
>sp|P46429|GST2_MANSE Glutathione S-transferase 2 (GST class-sigma)
Length = 203
Score = 93.6 bits (231), Expect = 4e-19
Identities = 55/171 (32%), Positives = 93/171 (54%), Gaps = 9/171 (5%)
Frame = +1
Query: 31 YFNARGRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEGKKLTQSMAI 210
YF A+G A L+ + EFED R+ EQW + KP TP G++PV+E +GKK QS+AI
Sbjct: 8 YFGAKGWARPTMLLAYGGQ-EFEDHRVEYEQWPEFKPNTPFGQMPVLEIDGKKYAQSLAI 66
Query: 211 ARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKIYFAPEEKKEDLRTEFVTTSGRD 390
+R + RKYG G D +E + I++ + + D+ + + + ++++ E +
Sbjct: 67 SRYLGRKYGLAGNDIEEDFEIDQIVDFVNDIRASAASVEYEQDAANKEVKHE-------E 119
Query: 391 NLKSLEGF---------IKESGFFVGEKITLAELFFVVLTDYLEKLPKLYD 516
N+K+ F K +GF ++T A+ FV + DYL+K+ ++ D
Sbjct: 120 NMKNKYPFQLNKLSEIITKNNGFLALGRLTWADFVFVGMFDYLKKMLRMPD 170
>sp|O35543|PTGD2_RAT Glutathione-requiring prostaglandin D synthase
(Glutathione-dependent PGD synthetase) (Prostaglandin-H2
D-isomerase) (Hematopoietic prostaglandin D synthase)
(H-PGDS)
Length = 199
Score = 93.2 bits (230), Expect = 6e-19
Identities = 52/160 (32%), Positives = 87/160 (54%), Gaps = 3/160 (1%)
Frame = +1
Query: 22 KVTYFNARGRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEGKKLTQS 201
K+ YFN RGRAEIIR + +++ED R+ + W +KPT P GK+PV+E EG L QS
Sbjct: 5 KLLYFNMRGRAEIIRYIFAYLDIKYEDHRIEQADWPKIKPTLPFGKIPVLEVEGLTLHQS 64
Query: 202 MAIARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKIYFAPEEK---KEDLRTEFV 372
+AIAR + + G+ + E + + +VD L+ ++ EE KE + +
Sbjct: 65 LAIARYLTKNTDLAGKTELE----QCQVDAVVDTLDDFMSLFPWAEENQDLKERTFNDLL 120
Query: 373 TTSGRDNLKSLEGFIKESGFFVGEKITLAELFFVVLTDYL 492
T LK L+ ++ + +F+G +T A+ ++ + + L
Sbjct: 121 TRQAPHLLKDLDTYLGDKEWFIGNYVTWADFYWDICSTTL 160
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 74,820,815
Number of Sequences: 369166
Number of extensions: 1480452
Number of successful extensions: 4365
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4183
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4282
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 5733423530
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)