Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_022_J04
(558 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q04820|MDHC_ECHGR Malate dehydrogenase, cytoplasmic 206 3e-53
sp|P11708|MDHC_PIG Malate dehydrogenase, cytoplasmic 204 2e-52
sp|P40925|MDHC_HUMAN Malate dehydrogenase, cytoplasmic 201 1e-51
sp|P14152|MDHC_MOUSE Malate dehydrogenase, cytoplasmic 199 3e-51
sp|P93819|MDHC1_ARATH Malate dehydrogenase, cytoplasmic 1 194 2e-49
sp|P57106|MDHD_ARATH Malate dehydrogenase, cytoplasmic 2 192 4e-49
sp|O24047|MDHC_MESCR Malate dehydrogenase, cytoplasmic 192 4e-49
sp|P61976|MDH_MYCPA Malate dehydrogenase 191 1e-48
sp|Q08062|MDHC_MAIZE Malate dehydrogenase, cytoplasmic 189 4e-48
sp|Q9SML8|MDHC_BETVU Malate dehydrogenase, cytoplasmic 187 1e-47
>sp|Q04820|MDHC_ECHGR Malate dehydrogenase, cytoplasmic
Length = 332
Score = 206 bits (524), Expect = 3e-53
Identities = 106/183 (57%), Positives = 125/183 (68%), Gaps = 3/183 (1%)
Frame = +2
Query: 17 VVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCIIWGNH 196
VVGNPANTNCLIMSKYAPSIPKENF+ALTRLDHNRAI Q+A KAGVP V+ IWGNH
Sbjct: 128 VVGNPANTNCLIMSKYAPSIPKENFTALTRLDHNRAIYQVAAKAGVPNTCVKNVCIWGNH 187
Query: 197 SNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVIXXXXXXXXXXX 376
SN Q+PD H +V DGK+ + + DE W+K F+ VQ RGAAVI
Sbjct: 188 SNKQFPDLSHAVVT-KDGKQHPAKELINDEKWVKEVFIPCVQNRGAAVIGLRKLSRAASA 246
Query: 377 XXXXXDHCRDWFHGTK--EWVSMAVYSDGS-YGVPKDVIFSFPVTIEHGKWHIVQGLTID 547
D RDW+ GTK EWVSM+VYS G YG PKD+ FSFPVTI+ G + +V GL++D
Sbjct: 247 AKAIVDQMRDWWFGTKEGEWVSMSVYSTGDHYGAPKDIYFSFPVTIKDGHYKVVDGLSMD 306
Query: 548 DIS 556
+ S
Sbjct: 307 EWS 309
>sp|P11708|MDHC_PIG Malate dehydrogenase, cytoplasmic
Length = 334
Score = 204 bits (518), Expect = 2e-52
Identities = 110/183 (60%), Positives = 127/183 (69%), Gaps = 3/183 (1%)
Frame = +2
Query: 17 VVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCIIWGNH 196
VVGNPANTNCL SK APSIPKENFS LTRLDHNRA AQIA K GV D V+ IIWGNH
Sbjct: 128 VVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTSDDVKNVIIWGNH 187
Query: 197 SNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVIXXXXXXXXXXX 376
S+TQYPD +H VK KEV V +AVKD++WLK +F+ TVQ RGAAVI
Sbjct: 188 SSTQYPDVNHAKVK-LQAKEVGVYEAVKDDSWLKGEFITTVQQRGAAVIKARKLSSAMSA 246
Query: 377 XXXXXDHCRDWFHGTK--EWVSMAVYSDG-SYGVPKDVIFSFPVTIEHGKWHIVQGLTID 547
DH RD + GT E+VSM + SDG SYGVP D+++SFPVTI+ W IV+GL I+
Sbjct: 247 AKAICDHVRDIWFGTPEGEFVSMGIISDGNSYGVPDDLLYSFPVTIKDKTWKIVEGLPIN 306
Query: 548 DIS 556
D S
Sbjct: 307 DFS 309
>sp|P40925|MDHC_HUMAN Malate dehydrogenase, cytoplasmic
Length = 334
Score = 201 bits (510), Expect = 1e-51
Identities = 108/183 (59%), Positives = 127/183 (69%), Gaps = 3/183 (1%)
Frame = +2
Query: 17 VVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCIIWGNH 196
VVGNPANTNCL SK APSIPKENFS LTRLDHNRA AQIA K GV + V+ IIWGNH
Sbjct: 128 VVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNH 187
Query: 197 SNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVIXXXXXXXXXXX 376
S+TQYPD +H VK GKEV V +A+KD++WLK +F+ TVQ RGAAVI
Sbjct: 188 SSTQYPDVNHAKVK-LQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSA 246
Query: 377 XXXXXDHCRDWFHGTK--EWVSMAVYSDG-SYGVPKDVIFSFPVTIEHGKWHIVQGLTID 547
DH RD + GT E+VSM V SDG SYGVP D+++SFPV I++ W V+GL I+
Sbjct: 247 AKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPIN 306
Query: 548 DIS 556
D S
Sbjct: 307 DFS 309
>sp|P14152|MDHC_MOUSE Malate dehydrogenase, cytoplasmic
Length = 334
Score = 199 bits (507), Expect = 3e-51
Identities = 107/183 (58%), Positives = 126/183 (68%), Gaps = 3/183 (1%)
Frame = +2
Query: 17 VVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCIIWGNH 196
VVGNPANTNCL SK APSIPKENFS LTRLDHNRA +QIA K GV D V+ IIWGNH
Sbjct: 128 VVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKSQIALKLGVTADDVKNVIIWGNH 187
Query: 197 SNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVIXXXXXXXXXXX 376
S+TQYPD +H VK GKEV V +A+KD++WLK +F+ TVQ RGAAVI
Sbjct: 188 SSTQYPDVNHAKVK-LQGKEVGVYEALKDDSWLKGEFITTVQQRGAAVIKARKLSSAMSA 246
Query: 377 XXXXXDHCRDWFHGTK--EWVSMAVYSDG-SYGVPKDVIFSFPVTIEHGKWHIVQGLTID 547
DH RD + GT E+VSM V SDG SYGVP D+++S PV I++ W V+GL I+
Sbjct: 247 AKAIADHIRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSLPVVIKNKTWKFVEGLPIN 306
Query: 548 DIS 556
D S
Sbjct: 307 DFS 309
>sp|P93819|MDHC1_ARATH Malate dehydrogenase, cytoplasmic 1
Length = 332
Score = 194 bits (492), Expect = 2e-49
Identities = 97/182 (53%), Positives = 121/182 (66%), Gaps = 2/182 (1%)
Frame = +2
Query: 17 VVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCIIWGNH 196
VV NPANTN LI+ ++APSIP++N S LTRLDHNRA+ QI+ + VP V+ IIWGNH
Sbjct: 129 VVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLSVPVSDVKNVIIWGNH 188
Query: 197 SNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVIXXXXXXXXXXX 376
S++QYPD +H V+ G E V + VKD+ WL +F+ TVQ RGAA+I
Sbjct: 189 SSSQYPDVNHAKVQTSSG-EKPVRELVKDDAWLDGEFISTVQQRGAAIIKARKLSSALSA 247
Query: 377 XXXXXDHCRDWFHGTKE--WVSMAVYSDGSYGVPKDVIFSFPVTIEHGKWHIVQGLTIDD 550
DH RDW GT E +VSM VYSDGSY VP +I+SFPVT +G W IVQGL ID+
Sbjct: 248 ASSACDHIRDWVLGTPEGTFVSMGVYSDGSYSVPSGLIYSFPVTCRNGDWSIVQGLPIDE 307
Query: 551 IS 556
+S
Sbjct: 308 VS 309
>sp|P57106|MDHD_ARATH Malate dehydrogenase, cytoplasmic 2
Length = 332
Score = 192 bits (489), Expect = 4e-49
Identities = 97/182 (53%), Positives = 121/182 (66%), Gaps = 2/182 (1%)
Frame = +2
Query: 17 VVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCIIWGNH 196
VV NPANTN LI+ ++APSIP++N + LTRLDHNRA+ Q++ + VP V+ IIWGNH
Sbjct: 129 VVANPANTNALILKEFAPSIPEKNITCLTRLDHNRALGQVSERLSVPVSDVKNVIIWGNH 188
Query: 197 SNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVIXXXXXXXXXXX 376
S+TQYPD +H VK G E V + VK++ WL +F+ TVQ RGAA+I
Sbjct: 189 SSTQYPDVNHATVKTSVG-EKPVRELVKNDEWLNGEFISTVQQRGAAIIKARKLSSALSA 247
Query: 377 XXXXXDHCRDWFHGTKE--WVSMAVYSDGSYGVPKDVIFSFPVTIEHGKWHIVQGLTIDD 550
DH RDW GT E +VSM VYSDGSY VP +I+SFPVT +G+W IVQGL IDD
Sbjct: 248 ASSACDHIRDWVVGTPEGTFVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLPIDD 307
Query: 551 IS 556
S
Sbjct: 308 AS 309
>sp|O24047|MDHC_MESCR Malate dehydrogenase, cytoplasmic
Length = 332
Score = 192 bits (489), Expect = 4e-49
Identities = 97/182 (53%), Positives = 119/182 (65%), Gaps = 2/182 (1%)
Frame = +2
Query: 17 VVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCIIWGNH 196
VV NPANTN LI+ ++APSIP++N S LTRLDHNRA+ QI+ + V V+ IIWGNH
Sbjct: 129 VVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVKNVIIWGNH 188
Query: 197 SNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVIXXXXXXXXXXX 376
S+TQYPD +H VK G + V + V D+ WL +F+ TVQ RGAA+I
Sbjct: 189 SSTQYPDVNHATVK-TQGVDKPVRELVADDAWLNGEFITTVQQRGAAIIKARKLSSALSA 247
Query: 377 XXXXXDHCRDWFHGTKE--WVSMAVYSDGSYGVPKDVIFSFPVTIEHGKWHIVQGLTIDD 550
DH DW GT E WVSM VYSDGSY VP +I+SFPVT ++G+W IVQGL IDD
Sbjct: 248 ASSACDHIHDWVLGTPEGTWVSMGVYSDGSYNVPAGIIYSFPVTCKNGEWTIVQGLPIDD 307
Query: 551 IS 556
S
Sbjct: 308 DS 309
>sp|P61976|MDH_MYCPA Malate dehydrogenase
Length = 329
Score = 191 bits (485), Expect = 1e-48
Identities = 99/183 (54%), Positives = 116/183 (63%), Gaps = 2/183 (1%)
Frame = +2
Query: 14 GVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCIIWGN 193
GV GNPANTN LI AP IP+E FSALTRLDHNRAI+Q+A K G +++ IWGN
Sbjct: 128 GVTGNPANTNALIAMSNAPDIpreRFSALTRLDHNRAISQLAKKTGAKVTDIKKMTIWGN 187
Query: 194 HSNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVIXXXXXXXXXX 373
HS TQYPD H VKG + EV V D+ W++NDF+ TV RGAA+I
Sbjct: 188 HSATQYPDIFHAEVKGKNAAEV-----VGDQNWIENDFIPTVAKRGAAIIDARGASSAAS 242
Query: 374 XXXXXXDHCRDWFHGTK--EWVSMAVYSDGSYGVPKDVIFSFPVTIEHGKWHIVQGLTID 547
D RDW GT +WVSMAV SDGSYGVP+ +I SFPVT + G W IVQGL ID
Sbjct: 243 AASATTDAARDWLLGTPAGDWVSMAVISDGSYGVPEGLISSFPVTTKDGDWTIVQGLEID 302
Query: 548 DIS 556
+ S
Sbjct: 303 EFS 305
>sp|Q08062|MDHC_MAIZE Malate dehydrogenase, cytoplasmic
Length = 332
Score = 189 bits (480), Expect = 4e-48
Identities = 96/182 (52%), Positives = 119/182 (65%), Gaps = 2/182 (1%)
Frame = +2
Query: 17 VVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCIIWGNH 196
VV NPANTN LI+ ++APSIP++N + LTRLDHNRA+ QI+ + V V+ IIWGNH
Sbjct: 129 VVANPANTNALILKEFAPSIPEKNVTCLTRLDHNRALGQISERLNVQVSDVKNVIIWGNH 188
Query: 197 SNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVIXXXXXXXXXXX 376
S++QYPD +H VK G E V + V D+ WL +F+ TVQ RGAA+I
Sbjct: 189 SSSQYPDVNHATVKTSTG-EKPVRELVSDDEWLNGEFITTVQQRGAAIIKARKFSSALSA 247
Query: 377 XXXXXDHCRDWFHGTKE--WVSMAVYSDGSYGVPKDVIFSFPVTIEHGKWHIVQGLTIDD 550
DH RDW GT E +VSM VYSDGSYGVP +I+SFPVT G+W IVQGL ID+
Sbjct: 248 ASSACDHIRDWVLGTPEGTFVSMGVYSDGSYGVPSGLIYSFPVTCSGGEWKIVQGLPIDE 307
Query: 551 IS 556
S
Sbjct: 308 FS 309
>sp|Q9SML8|MDHC_BETVU Malate dehydrogenase, cytoplasmic
Length = 332
Score = 187 bits (476), Expect = 1e-47
Identities = 94/182 (51%), Positives = 119/182 (65%), Gaps = 2/182 (1%)
Frame = +2
Query: 17 VVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCIIWGNH 196
VV NPANTN LI+ ++APSIP++N + LTRLDHNRA+ QI+ + V+ IIWGNH
Sbjct: 129 VVANPANTNALILKEFAPSIPEKNITCLTRLDHNRALGQISERLNAQVSDVKNVIIWGNH 188
Query: 197 SNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVIXXXXXXXXXXX 376
S++QYPD + VK G E +V + V D+ WL +F+ TVQ RGAA+I
Sbjct: 189 SSSQYPDVNPCTVKTGSG-EKAVRELVADDAWLNGEFITTVQQRGAAIIKARKLSSALSA 247
Query: 377 XXXXXDHCRDWFHGTKE--WVSMAVYSDGSYGVPKDVIFSFPVTIEHGKWHIVQGLTIDD 550
DH RDW GT E WVSM VYSDGSY VP +I+SFPVT + G+W IVQGL ID+
Sbjct: 248 ASSACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGIIYSFPVTCKDGEWKIVQGLPIDE 307
Query: 551 IS 556
+S
Sbjct: 308 VS 309
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 69,127,559
Number of Sequences: 369166
Number of extensions: 1410191
Number of successful extensions: 3727
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3534
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3601
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 3980545740
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)