Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_022_I14
(769 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P55112|NAS4_CAEEL Zinc metalloproteinase nas-4 precursor... 104 3e-22
sp|P31579|LCE_ORYLA Low choriolytic enzyme precursor (Hatch... 89 2e-17
sp|P31581|HCE21_ORYLA High choriolytic enzyme 2 precursor (... 88 3e-17
sp|P31580|HCE23_ORYLA High choriolytic enzyme 1 precursor (... 87 4e-17
sp|P07584|ASTA_ASTFL Astacin precursor (Crayfish small-mole... 86 8e-17
sp|Q18439|NAS8_CAEEL Zinc metalloproteinase nas-8 precursor... 86 1e-16
sp|P55113|NAS7_CAEEL Zinc metalloproteinase nas-7 precursor... 84 5e-16
sp|Q9XTD6|NAS12_CAEEL Zinc metalloproteinase nas-12 precurs... 83 7e-16
sp|P55115|NAS15_CAEEL Zinc metalloproteinase nas-15 precurs... 83 7e-16
sp|Q20191|NAS13_CAEEL Zinc metalloproteinase nas-13 precurs... 82 1e-15
>sp|P55112|NAS4_CAEEL Zinc metalloproteinase nas-4 precursor (Nematode astacin 4)
Length = 315
Score = 104 bits (259), Expect = 3e-22
Identities = 58/169 (34%), Positives = 96/169 (56%), Gaps = 5/169 (2%)
Frame = +3
Query: 177 KRWPNNQVLFSFAPAFPNDKKGIVRECLVELQKDLGNCVKFSESSASHYIEVQSL--DQG 350
+RWPNN++ ++ + + + + ++ + E CVKF S + + + D+G
Sbjct: 102 RRWPNNEIPYTLSSQYGSYARSVIANAMNEYHTK--TCVKFVARDPSKHHDYLWIHPDEG 159
Query: 351 CYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHINVKWDRIL 530
CYSL+G TGG KQP++L + GC+ GT+ HE +HA+GF H R+DRD++I+V W ++
Sbjct: 160 CYSLVGKTGG-KQPVSLDS-GCI-QVGTIVHELMHAVGFFHEQSRQDRDSYIDVVWQNVM 216
Query: 531 TSHCSQFVKCD---GCEVDGPYETHSVMHYPSYGFACVPGENVVFKRDG 668
QF K + +D PY+ S+MHY Y F+ + +V K+ G
Sbjct: 217 NGADDQFEKYNLNVISHLDEPYDYASIMHYGPYAFSGSGKKTLVPKKSG 265
>sp|P31579|LCE_ORYLA Low choriolytic enzyme precursor (Hatching enzyme zinc-protease LCE
subunit) (Choriolysin L)
Length = 271
Score = 88.6 bits (218), Expect = 2e-17
Identities = 60/190 (31%), Positives = 96/190 (50%), Gaps = 11/190 (5%)
Frame = +3
Query: 90 LNNMQM-----GDMVLDKSDIALINGFKAHYSNTKRWPNN-----QVLFSFAPAFPNDKK 239
+NN M GD+VL K+ A+ F A ++ RWP + +V + + + +D+K
Sbjct: 52 MNNNSMEELLEGDLVLPKTRNAM-KCFGA--PDSCRWPKSSNGIVKVPYVVSDNYESDEK 108
Query: 240 GIVRECLVELQKDLGNCVKF-SESSASHYIEVQSLDQGCYSLLGYTGGPKQPLNLQNPGC 416
+R + E + C+ F ++ Y+ ++ GC S++GY G KQ + LQ GC
Sbjct: 109 ETIRNAMKEFAEK--TCIHFVPRNNERAYLSLEPRF-GCKSMMGYVGD-KQVVVLQRFGC 164
Query: 417 MYSKGTVKHEFIHALGFMHTHMRKDRDNHINVKWDRILTSHCSQFVKCDGCEVDGPYETH 596
+ ++HE +HALGF H H R DRD H+ + W+ I+ F K D + PY+
Sbjct: 165 I-KHAVIQHELLHALGFYHEHTRSDRDQHVKINWENIIKDFTHNFDKNDTDNLGTPYDYG 223
Query: 597 SVMHYPSYGF 626
S+MHY F
Sbjct: 224 SIMHYGRTAF 233
>sp|P31581|HCE21_ORYLA High choriolytic enzyme 2 precursor (Hatching enzyme zinc-protease
HCE 2 subunit) (Choriolysin H 2)
Length = 279
Score = 87.8 bits (216), Expect = 3e-17
Identities = 47/123 (38%), Positives = 70/123 (56%), Gaps = 1/123 (0%)
Frame = +3
Query: 288 CVKFSESSASH-YIEVQSLDQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALG 464
C++F + + +I V S + GCYS LG GG +Q L+L GCMYS G ++HE HALG
Sbjct: 129 CIRFVRRTNEYDFISVVSKN-GCYSELGRKGG-QQELSLNRGGCMYS-GIIQHELNHALG 185
Query: 465 FMHTHMRKDRDNHINVKWDRILTSHCSQFVKCDGCEVDGPYETHSVMHYPSYGFACVPGE 644
F H R DRD+++ + W I+ + F K D ++ PY+ S+MHY F+ G
Sbjct: 186 FQHEQTRSDRDSYVRINWQNIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFSIAYGR 245
Query: 645 NVV 653
+ +
Sbjct: 246 DSI 248
>sp|P31580|HCE23_ORYLA High choriolytic enzyme 1 precursor (Hatching enzyme zinc-protease
HCE 1 subunit) (Choriolysin H 1)
Length = 270
Score = 87.4 bits (215), Expect = 4e-17
Identities = 46/123 (37%), Positives = 70/123 (56%), Gaps = 1/123 (0%)
Frame = +3
Query: 288 CVKFSESSASH-YIEVQSLDQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALG 464
C++F + + +I V S GCYS LG GG +Q L++ GCMYS G ++HE HALG
Sbjct: 120 CIRFVRRTNEYDFISVVS-KTGCYSELGRKGG-QQELSINRGGCMYS-GIIQHELNHALG 176
Query: 465 FMHTHMRKDRDNHINVKWDRILTSHCSQFVKCDGCEVDGPYETHSVMHYPSYGFACVPGE 644
F H R DRD+++ + W+ I+ + F K D ++ PY+ S+MHY F+ G
Sbjct: 177 FQHEQTRSDRDSYVRINWENIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFSIAYGR 236
Query: 645 NVV 653
+ +
Sbjct: 237 DSI 239
>sp|P07584|ASTA_ASTFL Astacin precursor (Crayfish small-molecule proteinase)
Length = 251
Score = 86.3 bits (212), Expect = 8e-17
Identities = 49/151 (32%), Positives = 83/151 (54%), Gaps = 2/151 (1%)
Frame = +3
Query: 183 WPNNQVLFSFAPAFPNDKKGIVRECLVELQKDLGNCVKF-SESSASHYIEVQSLDQGCYS 359
W + ++FA D+ I+ + EL++ C++F ++ S Y+E+ + GC+S
Sbjct: 59 WSGGVIPYTFAGVSGADQSAILSG-MQELEEK--TCIRFVPRTTESDYVEIFTSGSGCWS 115
Query: 360 LLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHINVKWDRILTSH 539
+G G +Q ++LQ GC+Y GT+ HE +HA+GF H H R DRDN++ + + + S
Sbjct: 116 YVGRISGAQQ-VSLQANGCVYH-GTIIHELMHAIGFYHEHTRMDRDNYVTINYQNVDPSM 173
Query: 540 CSQF-VKCDGCEVDGPYETHSVMHYPSYGFA 629
S F + V Y+ +S+MHY Y F+
Sbjct: 174 TSNFDIDTYSRYVGEDYQYYSIMHYGKYSFS 204
>sp|Q18439|NAS8_CAEEL Zinc metalloproteinase nas-8 precursor (Nematode astacin 8)
Length = 403
Score = 85.9 bits (211), Expect = 1e-16
Identities = 63/190 (33%), Positives = 96/190 (50%), Gaps = 11/190 (5%)
Frame = +3
Query: 168 SNTKRWPNNQVLFSFAPAFPNDKKGIVRECLVELQKDLGNCVKFSESSA--SHYIEVQSL 341
+ T++WPN ++ + + + ND++ V + D CV+F +A + Y+ + +
Sbjct: 116 TGTRKWPNGRIPYVISNQY-NDRERAVLARSFQAYHDK-TCVRFVPRTAVDNDYLYIGKI 173
Query: 342 DQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHINVKWD 521
D GCYS +G GG +Q L+L N GC+ T HE +H++GF H H R DRD HI + W
Sbjct: 174 D-GCYSDVGRAGG-RQELSLDN-GCL-QYDTAIHELMHSVGFYHEHERWDRDEHITILWH 229
Query: 522 RILTSHCSQFVKCDGCE---VDGPYETHSVMHYPSYGFACVPGENVVFKRD------GGL 674
I QF K D E Y+ +S+MHY S F+ E +V K+ G
Sbjct: 230 NIDREAYDQFGKVDLAESSYYGQLYDYYSIMHYDSLAFSKNGFETMVAKQSEMTAVIGAA 289
Query: 675 IDYNACYIKK 704
ID++ I K
Sbjct: 290 IDFSPIDILK 299
>sp|P55113|NAS7_CAEEL Zinc metalloproteinase nas-7 precursor (Nematode astacin 7)
Length = 382
Score = 83.6 bits (205), Expect = 5e-16
Identities = 55/175 (31%), Positives = 91/175 (52%), Gaps = 7/175 (4%)
Frame = +3
Query: 126 KSDIALINGFKAHYSN--TKRWPNNQVLFSFAPAFPNDKKGIVRECLVELQKDLGNCVKF 299
KSDI L K + + K WPN ++ ++ +P + ++ ++ + + + + C++F
Sbjct: 68 KSDIRLPRRHKRNGVSRAAKLWPNARIPYAISPHYSPHERALLAKAVKQYHEK--TCIRF 125
Query: 300 --SESSASHYIEVQSLDQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALGFMH 473
++ Y+ + +D GC+S +G T G Q L+L N GCM T+ HE +H +GF H
Sbjct: 126 VPRQTGEPDYLFIGKVD-GCFSEVGRTSGV-QVLSLDN-GCM-EYATIIHEMMHVVGFYH 181
Query: 474 THMRKDRDNHINVKWDRILTSHCSQFVKCDGCEVD---GPYETHSVMHYPSYGFA 629
H R DRDN I++ W I QF K D + PY+ S++HY S F+
Sbjct: 182 EHERWDRDNFIDIIWQNIDRGALDQFGKVDLSKTSYYGQPYDYKSILHYDSLAFS 236
>sp|Q9XTD6|NAS12_CAEEL Zinc metalloproteinase nas-12 precursor (Nematode astacin 12)
Length = 384
Score = 83.2 bits (204), Expect = 7e-16
Identities = 70/226 (30%), Positives = 105/226 (46%), Gaps = 16/226 (7%)
Frame = +3
Query: 6 FCL--LLVIATINYVAGQCGCPRQKLL-KRGLNNMQMGDMVL----------DKSDIALI 146
FCL LL+ I+ Q Q+L+ + + GDM+L K I
Sbjct: 11 FCLGYLLLFCKISNAVKQSWEINQELITEANKEHTVFGDMLLTPAQLIRYENSKDSDLSI 70
Query: 147 NGFKAHYSNTKRWPNNQVLFSFAPAFPNDKKGIVRECLVELQKDLGNCVKFSESSASH-Y 323
G S+ RW NN V + +P + +K I+ L ++ +C KF E + + Y
Sbjct: 71 RGVSIKGSSMNRWSNNIVPYVISPQYSPAQKQILVSSLRYFERV--SCFKFVERTTQNDY 128
Query: 324 IEVQSLDQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNH 503
+ + LD GCYS +G GG +Q L+L C+ + + HE +HA+GF H H R DRD+
Sbjct: 129 LFIVPLD-GCYSYVGKIGG-RQTLSLA-ADCI-ADYIIWHEMMHAIGFEHEHQRPDRDSF 184
Query: 504 INVKWDRILTSHCSQFVKCDGCEVDGP--YETHSVMHYPSYGFACV 635
I V + ++ F K V+ P Y+ S+MHY Y F V
Sbjct: 185 IRVDYANVIPGQMINFDKLKTSHVEYPDIYDFKSIMHYDGYAFGRV 230
>sp|P55115|NAS15_CAEEL Zinc metalloproteinase nas-15 precursor (Nematode astacin 15)
Length = 571
Score = 83.2 bits (204), Expect = 7e-16
Identities = 50/180 (27%), Positives = 93/180 (51%), Gaps = 5/180 (2%)
Frame = +3
Query: 153 FKAHYSNTKRWPNNQVLFSFAPAFPNDKKGIVRECLVELQKDLGNCVKF--SESSASHYI 326
+ A + + WP ++ ++ + + + + ++ + E C+++ E++ +Y+
Sbjct: 113 YNAIKNRLQLWPEGRIPYTISSQYSSYSRSLIAASMQEYASH--TCIRWVPKEAADVNYV 170
Query: 327 EVQSLDQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHI 506
+ D+GCYS++G GG KQ L+L + GC+ KG + HE +HA+GF H R DRD+HI
Sbjct: 171 HIYP-DRGCYSMVGKMGG-KQSLSLGS-GCI-QKGIILHELMHAVGFFHEQSRTDRDDHI 226
Query: 507 NVKWDRILTSHCSQFVKCDGCEVDG---PYETHSVMHYPSYGFACVPGENVVFKRDGGLI 677
+ W+ I QF K + Y+ S+MHY + F+ ++ K++G I
Sbjct: 227 TIMWNNIQAGMQGQFEKYGHGTIQSLGTGYDYGSIMHYGTKAFSRNGQPTMIPKKNGATI 286
>sp|Q20191|NAS13_CAEEL Zinc metalloproteinase nas-13 precursor (Nematode astacin 13)
Length = 527
Score = 82.4 bits (202), Expect = 1e-15
Identities = 51/155 (32%), Positives = 80/155 (51%), Gaps = 5/155 (3%)
Frame = +3
Query: 180 RWPNNQVLFSFAPAFPNDKKGIVRECLVELQKDLGNCVKFSESSASH--YIEVQSLDQGC 353
+W ++ ++ + + + + + E + E +K C+ FS SA YI + D GC
Sbjct: 195 KWEQARIPYTISSQYSSYSRSKIAEAIEEYRKK--TCIDFSPKSAGDLDYIHIVP-DDGC 251
Query: 354 YSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHINVKWDRILT 533
YSL+G GG KQP++L + GC+ KG + HE +HA+GF H R DRD ++ + W +
Sbjct: 252 YSLVGRIGG-KQPVSLGD-GCI-QKGIIIHELMHAVGFFHEQSRADRDEYVKINWSNVEA 308
Query: 534 SHCSQFVKCDGCEVD---GPYETHSVMHYPSYGFA 629
QF K +D Y+ SVMHY F+
Sbjct: 309 GLQDQFDKYSLNMIDHLGTKYDYGSVMHYAPTAFS 343
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 98,932,329
Number of Sequences: 369166
Number of extensions: 2178962
Number of successful extensions: 5020
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4776
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4962
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7115329200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)