Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_022_C02
(565 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O35490|BHMT_MOUSE Betaine--homocysteine S-methyltransferase 268 7e-72
sp|Q95332|BHMT_PIG Betaine--homocysteine S-methyltransferase 263 2e-70
sp|O09171|BHMT_RAT Betaine--homocysteine S-methyltransferase 263 3e-70
sp|Q93088|BHMT_HUMAN Betaine--homocysteine S-methyltransferase 261 6e-70
sp|Q9SDL7|HMT1_ARATH Homocysteine S-methyltransferase 1 (S-... 54 2e-07
sp|Q47690|MMUM_ECOLI Homocysteine S-methyltransferase (S-me... 44 4e-04
sp|Q55786|METH_SYNY3 Methionine synthase (5-methyltetrahydr... 38 0.015
sp|Q9FUM7|HMT4_MAIZE Homocysteine S-methyltransferase 4 (S-... 37 0.026
sp|P87138|YDM7_SCHPO Hypothetical protein C57A7.07c in chro... 37 0.044
sp|Q99707|METH_HUMAN Methionine synthase (5-methyltetrahydr... 35 0.098
>sp|O35490|BHMT_MOUSE Betaine--homocysteine S-methyltransferase
Length = 407
Score = 268 bits (685), Expect = 7e-72
Identities = 131/188 (69%), Positives = 148/188 (78%)
Frame = +1
Query: 1 GEVIIGDGGFVFQLEKRGYVKAGPWTPEAAVEHPEAVIQLHREFLRAGSDVMQAFTFYAS 180
GEV+IGDGGFVF LEKRGYVKAGPWTPEAAVEHPEAV QLHREFLRAGS+VMQ FTFYAS
Sbjct: 20 GEVVIGDGGFVFALEKRGYVKAGPWTPEAAVEHPEAVRQLHREFLRAGSNVMQTFTFYAS 79
Query: 181 DDKLENRGNEAASKYGGSNINRKACQLAKQVASEGQNILTLGGVSQTPSYLTGVGKAETQ 360
+DKLENRGN A K G +N AC +A+QVA EG + L GGVSQTPSYL+ + E +
Sbjct: 80 EDKLENRGNYVAEKISGQKVNEAACDIARQVADEG-DALVAGGVSQTPSYLSCKSEVEVK 138
Query: 361 NIFRKQFTVFKEEQVDFLLCEYFEHVEEAEWAVETAKEFNMPVACSLCIGPEGDMKNVPT 540
IFR+Q VF ++ VDFL+ EYFEHVEEA WAVE K PVA ++CIGPEGD+ VP
Sbjct: 139 KIFRQQLEVFMKKNVDFLIAEYFEHVEEAVWAVEALKASGKPVAATMCIGPEGDLHGVPP 198
Query: 541 GECAVRLV 564
GECAVRLV
Sbjct: 199 GECAVRLV 206
>sp|Q95332|BHMT_PIG Betaine--homocysteine S-methyltransferase
Length = 278
Score = 263 bits (673), Expect = 2e-70
Identities = 128/188 (68%), Positives = 147/188 (78%)
Frame = +1
Query: 1 GEVIIGDGGFVFQLEKRGYVKAGPWTPEAAVEHPEAVIQLHREFLRAGSDVMQAFTFYAS 180
GEV+IGDGGFVF LEKRGYVKAGPWTPEAAVEHPEAV QLHREFLRAGS+VMQ FTFYAS
Sbjct: 20 GEVVIGDGGFVFALEKRGYVKAGPWTPEAAVEHPEAVRQLHREFLRAGSNVMQTFTFYAS 79
Query: 181 DDKLENRGNEAASKYGGSNINRKACQLAKQVASEGQNILTLGGVSQTPSYLTGVGKAETQ 360
+DKLENRGN A K G +N AC +A+QVA EG + L GGVSQTPSYL+ + E +
Sbjct: 80 EDKLENRGNYVAEKISGQKVNEAACDIARQVADEG-DALVAGGVSQTPSYLSCKSETEVK 138
Query: 361 NIFRKQFTVFKEEQVDFLLCEYFEHVEEAEWAVETAKEFNMPVACSLCIGPEGDMKNVPT 540
+FR+Q VF ++ VDFL+ EYFEHVEEA WAVE K PVA ++CIGPEGD+ V
Sbjct: 139 KVFRQQLEVFMKKNVDFLIAEYFEHVEEAVWAVEALKASGKPVAATMCIGPEGDLHGVTP 198
Query: 541 GECAVRLV 564
G+CAVRLV
Sbjct: 199 GQCAVRLV 206
>sp|O09171|BHMT_RAT Betaine--homocysteine S-methyltransferase
Length = 407
Score = 263 bits (671), Expect = 3e-70
Identities = 128/188 (68%), Positives = 146/188 (77%)
Frame = +1
Query: 1 GEVIIGDGGFVFQLEKRGYVKAGPWTPEAAVEHPEAVIQLHREFLRAGSDVMQAFTFYAS 180
GEV+IGDGGFVF LEKRGYVKAGPWTPEAAVEHPEAV QLHREFLRAGS+VMQ FTFYAS
Sbjct: 20 GEVVIGDGGFVFALEKRGYVKAGPWTPEAAVEHPEAVRQLHREFLRAGSNVMQTFTFYAS 79
Query: 181 DDKLENRGNEAASKYGGSNINRKACQLAKQVASEGQNILTLGGVSQTPSYLTGVGKAETQ 360
+DKLENRGN A K G +N AC +A+QVA EG + L GGVSQTPSYL+ + E +
Sbjct: 80 EDKLENRGNYVAEKISGQKVNEAACDIARQVADEG-DALVAGGVSQTPSYLSCKSETEVK 138
Query: 361 NIFRKQFTVFKEEQVDFLLCEYFEHVEEAEWAVETAKEFNMPVACSLCIGPEGDMKNVPT 540
IF +Q VF ++ VDFL+ EYFEHVEEA WAVE K P+A ++CIGPEGD+ V
Sbjct: 139 KIFHQQLEVFMKKNVDFLIAEYFEHVEEAVWAVEALKTSGKPIAATMCIGPEGDLHGVSP 198
Query: 541 GECAVRLV 564
GECAVRLV
Sbjct: 199 GECAVRLV 206
>sp|Q93088|BHMT_HUMAN Betaine--homocysteine S-methyltransferase
Length = 406
Score = 261 bits (668), Expect = 6e-70
Identities = 127/188 (67%), Positives = 146/188 (77%)
Frame = +1
Query: 1 GEVIIGDGGFVFQLEKRGYVKAGPWTPEAAVEHPEAVIQLHREFLRAGSDVMQAFTFYAS 180
GE++IGDGGFVF LEKRGYVKAGPWTPEAAVEHPEAV QLHREFLRAGS+VMQ FTFYAS
Sbjct: 20 GEIVIGDGGFVFALEKRGYVKAGPWTPEAAVEHPEAVRQLHREFLRAGSNVMQTFTFYAS 79
Query: 181 DDKLENRGNEAASKYGGSNINRKACQLAKQVASEGQNILTLGGVSQTPSYLTGVGKAETQ 360
+DKLENRGN K G +N AC +A+QVA EG + L GGVSQTPSYL+ + E +
Sbjct: 80 EDKLENRGNYVLEKISGQEVNEAACDIARQVADEG-DALVAGGVSQTPSYLSCKSETEVK 138
Query: 361 NIFRKQFTVFKEEQVDFLLCEYFEHVEEAEWAVETAKEFNMPVACSLCIGPEGDMKNVPT 540
+F +Q VF ++ VDFL+ EYFEHVEEA WAVET PVA ++CIGPEGD+ VP
Sbjct: 139 KVFLQQLEVFMKKNVDFLIAEYFEHVEEAVWAVETLIASGKPVAATMCIGPEGDLHGVPP 198
Query: 541 GECAVRLV 564
GECAVRLV
Sbjct: 199 GECAVRLV 206
>sp|Q9SDL7|HMT1_ARATH Homocysteine S-methyltransferase 1 (S-methylmethionine:homocysteine
methyltransferase 1) (SMM:Hcy S-methyltransferase 1)
(AtHMT-1)
Length = 326
Score = 54.3 bits (129), Expect = 2e-07
Identities = 50/207 (24%), Positives = 85/207 (41%), Gaps = 25/207 (12%)
Frame = +1
Query: 1 GEVIIGDGGFVFQLEKRGYVKAGP-WTPEAAVEHPEAVIQLHREFLRAGSDVMQAFTFYA 177
G + DGGF QLE G P W+ + +++PE + ++H E+L AG+D++ ++ A
Sbjct: 18 GGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVHMEYLEAGADIVVTSSYQA 77
Query: 178 SDDKLENRGN------------------------EAASKYGGSNINRKACQLAKQVASEG 285
+ +RG E SK G + NR +A + S G
Sbjct: 78 TIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVSGHSYNR--ALVAASIGSYG 135
Query: 286 QNILTLGGVSQTPSYLTGVGKAETQNIFRKQFTVFKEEQVDFLLCEYFEHVEEAEWAVET 465
+ G + Y V + ++ R++ V E D L E + EA+ VE
Sbjct: 136 AYL--ADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFETIPNKLEAQACVEL 193
Query: 466 AKEFNMPVACSLCIGPEGDMKNVPTGE 546
+E + + +C D + P+GE
Sbjct: 194 LEEEKVQIPAWICF-TSVDGEKAPSGE 219
>sp|Q47690|MMUM_ECOLI Homocysteine S-methyltransferase (S-methylmethionine:homocysteine
methyltransferase)
Length = 310
Score = 43.5 bits (101), Expect = 4e-04
Identities = 42/167 (25%), Positives = 69/167 (41%), Gaps = 14/167 (8%)
Frame = +1
Query: 4 EVIIGDGGFVFQLEKRGYVKAGP-WTPEAAVEHPEAVIQLHREFLRAGSDVMQAFTFYAS 180
++++ DG +LE RG A W+ + VE+PE + ++H ++ RAG+ ++ A+
Sbjct: 15 DILLLDGAMATELEARGCNLADSLWSAKVLVENPELIREVHLDYYRAGAQCAITASYQAT 74
Query: 181 DDKLENRG-NEAASK--YGGS-NINRKACQLAKQVASEGQNILTLGGVSQTPSYLTGVGK 348
RG +EA SK G S + RKA + + +L G V +YL +
Sbjct: 75 PAGFAARGLDEAQSKALIGKSVELARKAREAYLAENPQAGTLLVAGSVGPYGAYLADGSE 134
Query: 349 ---------AETQNIFRKQFTVFKEEQVDFLLCEYFEHVEEAEWAVE 462
Q R + + D L CE + E E E
Sbjct: 135 YRGDYHCSVEAFQAFHRPRVEALLDAGADLLACETLPNFSEIEALAE 181
>sp|Q55786|METH_SYNY3 Methionine synthase (5-methyltetrahydrofolate--homocysteine
methyltransferase) (Methionine synthase, vitamin-B12
dependent isozyme) (MS)
Length = 1195
Score = 38.1 bits (87), Expect = 0.015
Identities = 24/61 (39%), Positives = 33/61 (54%)
Frame = +1
Query: 100 PEAVIQLHREFLRAGSDVMQAFTFYASDDKLENRGNEAASKYGGSNINRKACQLAKQVAS 279
PEAV +HR F AG+DV++ TF + L S Y +N+ A +LAK VA+
Sbjct: 54 PEAVATVHRAFYEAGADVVETDTFGGTPLVLAEYDLADQSYY----LNKAAAELAKAVAA 109
Query: 280 E 282
E
Sbjct: 110 E 110
>sp|Q9FUM7|HMT4_MAIZE Homocysteine S-methyltransferase 4 (S-methylmethionine:homocysteine
methyltransferase 4) (SMM:Hcy S-methyltransferase 4)
(ZmHMT-4)
Length = 342
Score = 37.4 bits (85), Expect = 0.026
Identities = 37/182 (20%), Positives = 74/182 (40%), Gaps = 24/182 (13%)
Frame = +1
Query: 1 GEVIIGDGGFVFQLEKRGY-VKAGPWTPEAAVEHPEAVIQLHREFLRAGSDVMQAFTFYA 177
G + DGG +LE G + W+ + P + ++H ++L AG+DV+ + ++ A
Sbjct: 22 GGCAVVDGGLGTELEAHGADLHDALWSAKCLASAPHLIRKVHLDYLEAGADVIISASYQA 81
Query: 178 SDDKLENRGNEAASKYGGSNINRKACQLAKQV----ASEGQNILTLG------------- 306
+ + ++RG S+ + R++ +A++ A+EG G
Sbjct: 82 TIEGFQSRG---FSRDESEELLRRSVHVAQEARRVFAAEGDRSSRRGRPPALVAASVGSY 138
Query: 307 ------GVSQTPSYLTGVGKAETQNIFRKQFTVFKEEQVDFLLCEYFEHVEEAEWAVETA 468
G + Y + K + +N R++ V D + E + EA+ E
Sbjct: 139 GAYRADGSEYSGDYGKSMTKEDLKNFHRRRLQVLAGAGPDLIAFETIPNKLEAQVYAELL 198
Query: 469 KE 474
+E
Sbjct: 199 EE 200
>sp|P87138|YDM7_SCHPO Hypothetical protein C57A7.07c in chromosome I
Length = 308
Score = 36.6 bits (83), Expect = 0.044
Identities = 18/45 (40%), Positives = 27/45 (60%), Gaps = 4/45 (8%)
Frame = +1
Query: 73 WTPEAAVEHPEAVIQLHREFLRAGSDVMQAFTFYAS----DDKLE 195
WT EA V +PE V++ H EFL+ D++ FT+ D+K+E
Sbjct: 25 WTSEALVRYPEIVVKHHEEFLKV-CDIISTFTYQLDASIYDEKVE 68
>sp|Q99707|METH_HUMAN Methionine synthase (5-methyltetrahydrofolate--homocysteine
methyltransferase) (Methionine synthase, vitamin-B12
dependent) (MS)
Length = 1265
Score = 35.4 bits (80), Expect = 0.098
Identities = 27/114 (23%), Positives = 54/114 (47%), Gaps = 4/114 (3%)
Frame = +1
Query: 88 AVEHPEAVIQLHREFLRAGSDVMQAFTFYASDDKLENRGNEAAS---KYGGSNINRKAC- 255
++ P+ + Q+H+E+L AG+D+++ TF ++ + G E + + + RKA
Sbjct: 71 SITQPDVIYQIHKEYLLAGADIIETNTFSSTSIAQADYGLEHLAYRMNMCSAGVARKAAE 130
Query: 256 QLAKQVASEGQNILTLGGVSQTPSYLTGVGKAETQNIFRKQFTVFKEEQVDFLL 417
++ Q + LG ++T S V + + +NI + +EQ LL
Sbjct: 131 EVTLQTGIKRFVAGALGPTNKTLSVSPSVERPDYRNITFDELVEAYQEQAKGLL 184
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.317 0.134 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 62,181,605
Number of Sequences: 369166
Number of extensions: 1196982
Number of successful extensions: 3602
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3511
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3595
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 4078830820
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)