Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_022_B04
(446 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P55404|TRBG_RHISN PROBABLE CONJUGAL TRANSFER PROTEIN TRB... 31 1.1
sp|Q8RAC2|ARGR_THETN Arginine repressor 30 1.9
sp|P21421|RPOB_PLAFA DNA-directed RNA polymerase beta chain 30 3.2
sp|Q8D3B5|SYFB_WIGBR Phenylalanyl-tRNA synthetase beta chai... 29 4.1
sp|Q8DHN4|ARGJ_SYNEL Arginine biosynthesis bifunctional pro... 29 4.1
sp|P76347|YEEJ_ECOLI Hypothetical protein yeeJ 29 4.1
sp|O74536|SNF1_SCHPO SNF1-like protein kinase ssp2 29 5.4
sp|Q8X8V7|YEEJ_ECO57 Hypothetical protein yeeJ 29 5.4
sp|O45435|YV4Q_CAEEL Hypothetical protein F32B6.9 in chromo... 28 7.1
sp|P17743|T2C2_HAEIN Type II restriction enzyme HincII (End... 28 9.2
>sp|P55404|TRBG_RHISN PROBABLE CONJUGAL TRANSFER PROTEIN TRBG preCURSOR
Length = 270
Score = 31.2 bits (69), Expect = 1.1
Identities = 18/54 (33%), Positives = 24/54 (44%)
Frame = -2
Query: 427 LKYHLSSLAVRRIFSYPSAIG*STARLTSSFLITGLPSRSTDKLEASNSIPNAA 266
LK H S R F YP + A + S G+P + DKL S S+ +A
Sbjct: 139 LKSHPSQYMARVGFEYPEDVSTKLADINSRLETGGIPGTAPDKLNFSYSVSGSA 192
>sp|Q8RAC2|ARGR_THETN Arginine repressor
Length = 151
Score = 30.4 bits (67), Expect = 1.9
Identities = 18/60 (30%), Positives = 30/60 (50%), Gaps = 3/60 (5%)
Frame = +3
Query: 129 DDNFKNAYSDM---QLNLSEALSMLRQLNIISLDQSSQLLTAKTLEQSAAALGIEFDASN 299
+D + Y+ M N+SE L L +I+++D + ++ KTL SA+A D N
Sbjct: 56 EDGKRYKYAPMTKVDTNISERLMTLLSESIVNVDYAGNIIVIKTLSGSASAAAEAIDTLN 115
>sp|P21421|RPOB_PLAFA DNA-directed RNA polymerase beta chain
Length = 1024
Score = 29.6 bits (65), Expect = 3.2
Identities = 12/28 (42%), Positives = 19/28 (67%)
Frame = -1
Query: 110 VSHKQFQIFLFNSIWLAKNVNISKQTLL 27
+ +K F FN+I+L KN+N +K T+L
Sbjct: 419 IFNKNFYNISFNNIYLKKNINFNKTTIL 446
>sp|Q8D3B5|SYFB_WIGBR Phenylalanyl-tRNA synthetase beta chain (Phenylalanine--tRNA ligase
beta chain) (PheRS)
Length = 803
Score = 29.3 bits (64), Expect = 4.1
Identities = 17/56 (30%), Positives = 28/56 (50%)
Frame = +3
Query: 45 YIHILGQPNGVKQEDLELLVRNWAIENCDDNFKNAYSDMQLNLSEALSMLRQLNII 212
Y+ I+G+ N Q L++ R + E DN KN Y N+S+ R ++I+
Sbjct: 664 YVGIIGEINPNIQNKLDIKNRIFVFELLWDNIKNFYVPKIYNISDIPFNYRDISIV 719
>sp|Q8DHN4|ARGJ_SYNEL Arginine biosynthesis bifunctional protein argJ [Includes:
Glutamate N-acetyltransferase (Ornithine
acetyltransferase) (Ornithine transacetylase) (OATase);
Amino-acid acetyltransferase (N-acetylglutamate
synthase) (AGS)] [Contains: Arginine biosynthesis
bifunctional protein argJ alpha chain; Arginine
biosynthesis bifunctional protein argJ beta chain]
Length = 419
Score = 29.3 bits (64), Expect = 4.1
Identities = 12/26 (46%), Positives = 19/26 (73%)
Frame = +3
Query: 261 SAAALGIEFDASNLSVDLDGNPVIRN 338
+A G+ FDASNL++ L G P++R+
Sbjct: 326 AAGRAGVPFDASNLAIALGGIPMMRH 351
>sp|P76347|YEEJ_ECOLI Hypothetical protein yeeJ
Length = 2358
Score = 29.3 bits (64), Expect = 4.1
Identities = 21/106 (19%), Positives = 48/106 (45%), Gaps = 2/106 (1%)
Frame = +3
Query: 102 VRNWAIENCDDNFKNAYSDMQLN--LSEALSMLRQLNIISLDQSSQLLTAKTLEQSAAAL 275
V++ + + DN +Y+ + +S L+++ QLN + ++ ++ ++ S
Sbjct: 578 VQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAVVNIISVSSSRTHS 637
Query: 276 GIEFDASNLSVDLDGNPVIRNDDVNRAVDQPIAEGYEKIRRTASED 413
I+ D L GNP+ ++ D+P+ E +++ S D
Sbjct: 638 SIKIDKDRY---LSGNPIEVTVELRDENDKPVKEQKQQLNNAVSID 680
>sp|O74536|SNF1_SCHPO SNF1-like protein kinase ssp2
Length = 576
Score = 28.9 bits (63), Expect = 5.4
Identities = 14/49 (28%), Positives = 24/49 (48%)
Frame = +3
Query: 69 NGVKQEDLELLVRNWAIENCDDNFKNAYSDMQLNLSEALSMLRQLNIIS 215
NG + D+ + W I +C KN Y+ ++L L E + L++ S
Sbjct: 487 NGKYRSDMYTIKSRWEIPHCKREGKNTYAYIELQLYEVMPGCFMLDVKS 535
>sp|Q8X8V7|YEEJ_ECO57 Hypothetical protein yeeJ
Length = 2660
Score = 28.9 bits (63), Expect = 5.4
Identities = 21/106 (19%), Positives = 48/106 (45%), Gaps = 2/106 (1%)
Frame = +3
Query: 102 VRNWAIENCDDNFKNAYSDMQLN--LSEALSMLRQLNIISLDQSSQLLTAKTLEQSAAAL 275
V++ + + DN +Y+ + +S L+++ QLN + ++ ++ ++ S
Sbjct: 578 VQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAVVNIISVSSSRTHS 637
Query: 276 GIEFDASNLSVDLDGNPVIRNDDVNRAVDQPIAEGYEKIRRTASED 413
I+ D L GNP+ ++ D+P+ E +++ S D
Sbjct: 638 SIKIDKDRY---LSGNPIEVTVELRDENDKPVKEQKQQLNTAVSID 680
>sp|O45435|YV4Q_CAEEL Hypothetical protein F32B6.9 in chromosome IV
Length = 413
Score = 28.5 bits (62), Expect = 7.1
Identities = 12/26 (46%), Positives = 18/26 (69%)
Frame = -3
Query: 297 WKRRILFLTPQQTVQVFWLSVIDLID 220
WK +++L +V+VF+L IDLID
Sbjct: 25 WKELLIYLILYYSVRVFYLKGIDLID 50
>sp|P17743|T2C2_HAEIN Type II restriction enzyme HincII (Endonuclease HincII) (R.HincII)
Length = 258
Score = 28.1 bits (61), Expect = 9.2
Identities = 23/80 (28%), Positives = 41/80 (51%), Gaps = 8/80 (10%)
Frame = +3
Query: 108 NWAIENCDDNFKNAYSDMQLNLSEALSML--RQLNIISLDQSSQLLTAKTLEQSAAALGI 281
NW+IEN + +N +D+ L + +L ++ NI Q+ +++A L Q+ A + I
Sbjct: 98 NWSIENLFEEKQNDTADILLVKDQFYELLDVKRRNISKSAQAPNIISAYKLAQTCAKM-I 156
Query: 282 E------FDASNLSVDLDGN 323
+ FD + L VD + N
Sbjct: 157 DNKEFDLFDINYLEVDSELN 176
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 48,741,483
Number of Sequences: 369166
Number of extensions: 860663
Number of successful extensions: 2501
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2444
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2501
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2394311040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)