Planarian EST Database


Dr_sW_022_B01

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_022_B01
         (626 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P90900|IFA4_CAEEL  Intermediate filament protein ifa-4 (I...    76   8e-14
sp|O02365|IFA2_CAEEL  Intermediate filament protein ifa-2 (I...    70   3e-12
sp|P23731|IFEB_ASCSU  Intermediate filament protein B (IF-B)       70   6e-12
sp|P90901|IFA1_CAEEL  Intermediate filament protein ifa-1 (I...    69   1e-11
sp|P31732|OV71_ONCVO  Muscle cell intermediate filament prot...    67   4e-11
sp|Q19289|IFB1_CAEEL  Intermediate filament protein ifb-1 (I...    67   5e-11
sp|Q21065|IFA3_CAEEL  Intermediate filament protein ifa-3 (I...    66   6e-11
sp|Q17107|AV71_ACAVI  Muscle cell intermediate filament prot...    64   2e-10
sp|P23730|IFEA_ASCSU  Intermediate filament protein A (IF-A)       64   3e-10
sp|Q19286|IFB2_CAEEL  Intermediate filament protein ifb-2 (I...    63   5e-10
>sp|P90900|IFA4_CAEEL Intermediate filament protein ifa-4 (Intermediate filament protein
           A4) (IF-A4) (Cel IF A4)
          Length = 575

 Score = 75.9 bits (185), Expect = 8e-14
 Identities = 46/130 (35%), Positives = 74/130 (56%), Gaps = 6/130 (4%)
 Frame = +1

Query: 166 ETTDYQSKRMKFRRTTKGNISIAETDLTGSFIILENIGTKVESLEGMTIKRAIEGNSNQI 345
           ETT + S    + R+ KGNI+I E + +G +I+LENI  + E++    ++R I G   +I
Sbjct: 453 ETTSHSS----YSRSAKGNIAIQEAEPSGKYIVLENISRRDENIGDWKLRRKIAG-KREI 507

Query: 346 SYTISSKVTLNAGEKISLVSRGKKTGKGLEIETD------EASWGVGNNVVTTSYDSEGV 507
            +T   + TL A + + + +R    G+G+    D      E S+G GN+VVTT Y+ EG 
Sbjct: 508 VFTFpreFTLRAQKSVKIFAR----GQGVHSPPDSLVYDLEDSFGTGNDVVTTLYNKEGE 563

Query: 508 EKAAHYQEVS 537
           ++A+H Q  S
Sbjct: 564 DRASHSQRAS 573
>sp|O02365|IFA2_CAEEL Intermediate filament protein ifa-2 (Intermediate filament protein
           A2) (IF-A2) (Cel IF A2)
          Length = 581

 Score = 70.5 bits (171), Expect = 3e-12
 Identities = 47/131 (35%), Positives = 70/131 (53%), Gaps = 4/131 (3%)
 Frame = +1

Query: 157 TERETTDYQSKRMKFRRTTKGNISIAETDLTGSFIILENI-GTKVESLEGMTIKRAIEGN 333
           T R      S R  ++R+ KGN++I ET   G F+ILEN    K E L    +KR I+G 
Sbjct: 447 TMRVVKGEHSSRTSYQRSAKGNVAIKETSPEGKFVILENTHRAKEEPLGDWKLKRKIDG- 505

Query: 334 SNQISYTISSKVTLNAGEKISLVSRGKKTGKGLEI---ETDEASWGVGNNVVTTSYDSEG 504
             +I +T  S   L+  + + + +RG+      E+   E DE ++GVG NV T  Y+++G
Sbjct: 506 KREIVFTFPSDYILHPFQSVKIFARGQGIANPPEVLIFEGDE-TFGVGANVQTILYNNKG 564

Query: 505 VEKAAHYQEVS 537
            E+A H Q  S
Sbjct: 565 EERATHIQRQS 575
>sp|P23731|IFEB_ASCSU Intermediate filament protein B (IF-B)
          Length = 589

 Score = 69.7 bits (169), Expect = 6e-12
 Identities = 43/120 (35%), Positives = 66/120 (55%), Gaps = 4/120 (3%)
 Frame = +1

Query: 184 SKRMKFRRTTKGNISIAETDLTGSFIILENI-GTKVESLEGMTIKRAIEGNSNQISYTIS 360
           S +  F+R+ KGN++I+E D  G FI LEN   +K E+L    +KR ++ N  +I YTI 
Sbjct: 465 STKTTFQRSAKGNVTISECDPNGKFITLENTHRSKDENLGEHRLKRKLD-NRREIVYTIP 523

Query: 361 SKVTLNAGEKISLVSR---GKKTGKGLEIETDEASWGVGNNVVTTSYDSEGVEKAAHYQE 531
               L AG  + + +R   G        +   E +WG+G NVVT+  + +G E+A H Q+
Sbjct: 524 PNTVLKAGRTMKIYARDQGGIHNPPDTLVFDGENTWGIGANVVTSLINKDGDERATHTQK 583
>sp|P90901|IFA1_CAEEL Intermediate filament protein ifa-1 (Intermediate filament protein
           A1) (IF-A1) (Cel IF A1)
          Length = 575

 Score = 68.6 bits (166), Expect = 1e-11
 Identities = 43/130 (33%), Positives = 66/130 (50%), Gaps = 3/130 (2%)
 Frame = +1

Query: 157 TERETTDYQSKRMKFRRTTKGNISIAETDLTGSFIILENI-GTKVESLEGMTIKRAIEGN 333
           T R      + R  F+R+ KGN+SI E    G FI+L+N    K E++    +KR I+G 
Sbjct: 445 TMRVVKGETASRQSFQRSAKGNVSIHEASPDGKFIVLQNTHRAKDEAIGEWKLKRRIDGK 504

Query: 334 SNQISYTISSKVTLNAGEKISLVSRGKKTGKGLE--IETDEASWGVGNNVVTTSYDSEGV 507
              + YT+     L AG+ + + +R +      +  +   E S+G GNNV T  ++ EG 
Sbjct: 505 RENV-YTLPRDFVLRAGKTLKIFARNQGVASPPDQLVYDAEDSFGSGNNVQTILFNKEGE 563

Query: 508 EKAAHYQEVS 537
           E+A H Q  S
Sbjct: 564 ERATHIQRQS 573
>sp|P31732|OV71_ONCVO Muscle cell intermediate filament protein OV71
          Length = 432

 Score = 67.0 bits (162), Expect = 4e-11
 Identities = 42/121 (34%), Positives = 66/121 (54%), Gaps = 3/121 (2%)
 Frame = +1

Query: 184 SKRMKFRRTTKGNISIAETDLTGSFIILENI-GTKVESLEGMTIKRAIEGNSNQISYTIS 360
           + R  F+R+ KGN+SI +    G FI+LEN   +K E +    +KR I+G   +I YT  
Sbjct: 309 ASRTSFQRSAKGNVSIQDASSDGKFILLENTHRSKEEPIGEWRLKRKIDG-KREIVYTFP 367

Query: 361 SKVTLNAGEKISLVSRGKKTGKGLE--IETDEASWGVGNNVVTTSYDSEGVEKAAHYQEV 534
               L  G+ + + +RG+      +  +   E S+GVG+NV T  ++ EG E+A+H Q  
Sbjct: 368 RDFILKPGKTVKIWARGQGVYSPPDQLVFDAEDSFGVGSNVQTILFNKEGEERASHIQRS 427

Query: 535 S 537
           S
Sbjct: 428 S 428
>sp|Q19289|IFB1_CAEEL Intermediate filament protein ifb-1 (Intermediate filament protein
           B1) (IF-B1) (Cel IF B1)
          Length = 589

 Score = 66.6 bits (161), Expect = 5e-11
 Identities = 40/120 (33%), Positives = 68/120 (56%), Gaps = 4/120 (3%)
 Frame = +1

Query: 184 SKRMKFRRTTKGNISIAETDLTGSFIILENI-GTKVESLEGMTIKRAIEGNSNQISYTIS 360
           S +  F+R+ KGN++I+E D  G FI LEN    K E++    I+R ++G   +I Y+I 
Sbjct: 465 STKTTFQRSAKGNVTISECDPNGKFIKLENSHRNKDENVGEHKIRRKLDGR-REIVYSIP 523

Query: 361 SKVTLNAGEKISLVSR---GKKTGKGLEIETDEASWGVGNNVVTTSYDSEGVEKAAHYQE 531
           + V +  G+ +++ +R   G        +   E +WG+G NVVT+  + +G E+A H Q+
Sbjct: 524 ANVVIKPGKNLTIYARDQGGINNPPESLVFDGENTWGIGANVVTSLVNKDGEERATHTQK 583
>sp|Q21065|IFA3_CAEEL Intermediate filament protein ifa-3 (Intermediate filament protein
           A3) (IF-A3) (Cel IF A3)
          Length = 581

 Score = 66.2 bits (160), Expect = 6e-11
 Identities = 44/130 (33%), Positives = 64/130 (49%), Gaps = 3/130 (2%)
 Frame = +1

Query: 157 TERETTDYQSKRMKFRRTTKGNISIAETDLTGSFIILENI-GTKVESLEGMTIKRAIEGN 333
           T R      S R  ++R+ KGN+SI E    G F+ILEN    K E L    +KR I+G 
Sbjct: 447 TMRVVKGEHSSRTSYQRSAKGNVSIKEVSPEGKFVILENTHRDKEEPLGDWKLKRKIDG- 505

Query: 334 SNQISYTISSKVTLNAGEKISLVSRGKKTGKGLEIETDEA--SWGVGNNVVTTSYDSEGV 507
             +I +T  S   L+  + + + +RG       E+   E   ++G G NV T  Y++ G 
Sbjct: 506 KREIVFTFPSDYILHPVQTVKIFARGNGVANPPEVLVFEGDDTFGAGANVQTILYNNSGE 565

Query: 508 EKAAHYQEVS 537
           E+A H Q  S
Sbjct: 566 ERATHMQRQS 575
>sp|Q17107|AV71_ACAVI Muscle cell intermediate filament protein AV71
          Length = 394

 Score = 64.3 bits (155), Expect = 2e-10
 Identities = 39/121 (32%), Positives = 66/121 (54%), Gaps = 3/121 (2%)
 Frame = +1

Query: 184 SKRMKFRRTTKGNISIAETDLTGSFIILENIG-TKVESLEGMTIKRAIEGNSNQISYTIS 360
           + R  F+R+ KGN+SI +    G +I+ EN   +K E +    +KR I+G   +I YT  
Sbjct: 271 ASRTSFQRSAKGNVSIQDAASDGKYILSENTHRSKEEPIGEWRLKRKIDGK-REIVYTFP 329

Query: 361 SKVTLNAGEKISLVSRGKKTGKGLE--IETDEASWGVGNNVVTTSYDSEGVEKAAHYQEV 534
           +   L  G+ + + +RG+      +  +   E S+G+G+NV T  ++ EG E+A+H Q  
Sbjct: 330 TNFVLKPGKSVKIWARGQGVYSPPDQLVFDAEDSFGIGSNVQTILFNKEGEERASHIQRS 389

Query: 535 S 537
           S
Sbjct: 390 S 390
>sp|P23730|IFEA_ASCSU Intermediate filament protein A (IF-A)
          Length = 497

 Score = 63.9 bits (154), Expect = 3e-10
 Identities = 41/125 (32%), Positives = 68/125 (54%), Gaps = 7/125 (5%)
 Frame = +1

Query: 184 SKRMKFRRTTKGNISIAETDLTGSFIILENI-GTKVESLEGMTIKRAIEGNSNQISYTIS 360
           + R  F+R+ KGN+SI ET   G +++LEN   +K E++    +KR I+G   +I YT+ 
Sbjct: 374 ASRTSFQRSAKGNVSIQETAPDGRYVVLENTHRSKEEAIGEWKLKRKIDG-KREIVYTLP 432

Query: 361 SKVTLNAGEKISLVSRGKKTGKGLEIETD------EASWGVGNNVVTTSYDSEGVEKAAH 522
               L  G+ + + +RG+    G+    +      E ++G G+NV T  ++ EG E+A H
Sbjct: 433 RDFILRPGKSVKIWARGQ---GGIHSPPEQLVFDLEDTFGSGSNVQTILFNREGEERATH 489

Query: 523 YQEVS 537
            Q  S
Sbjct: 490 IQRSS 494
>sp|Q19286|IFB2_CAEEL Intermediate filament protein ifb-2 (Intermediate filament protein
           B2) (IF-B2) (Cel IF B2)
          Length = 543

 Score = 63.2 bits (152), Expect = 5e-10
 Identities = 40/121 (33%), Positives = 58/121 (47%), Gaps = 4/121 (3%)
 Frame = +1

Query: 190 RMKFRRTTKGNISIAETDLTGSFIILENI-GTKVESLEGMTIKRAIEGNSNQISYTISSK 366
           R  F+R  KGN+SI E D  G +IILEN  G+  E +    I+R I+G      + + S 
Sbjct: 421 RSSFKRHAKGNVSIVECDPQGKYIILENTSGSVAEDVSNFEIRRVIDG-VQAFVFRLPSH 479

Query: 367 VTLNAGEKISLVSR---GKKTGKGLEIETDEASWGVGNNVVTTSYDSEGVEKAAHYQEVS 537
           + +     + +  R   G  +     +     SWG G  V T  Y+S G+EKA+H Q   
Sbjct: 480 LVIQQHGHLKIYGRNSGGINSPPDSIVMESHPSWGQGGQVETFLYNSHGIEKASHIQTTV 539

Query: 538 A 540
           A
Sbjct: 540 A 540
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.315    0.130    0.352 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 45,799,719
Number of Sequences: 369166
Number of extensions: 733260
Number of successful extensions: 2220
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2165
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2201
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 4974853140
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)