Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_022_A08
(789 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8HXY0|ETFA_MACFA Electron transfer flavoprotein alpha-s... 176 9e-44
sp|P13804|ETFA_HUMAN Electron transfer flavoprotein alpha-s... 175 1e-43
sp|Q99LC5|ETFA_MOUSE Electron transfer flavoprotein alpha-s... 175 1e-43
sp|Q5RC31|ETFA_PONPY Electron transfer flavoprotein alpha-s... 173 6e-43
sp|P13803|ETFA_RAT Electron transfer flavoprotein alpha-sub... 172 1e-42
sp|Q93615|ETFA_CAEEL Probable electron transfer flavoprotei... 171 3e-42
sp|Q8J0W1|ETFA_CRYNE Probable electron transfer flavoprotei... 137 3e-32
sp|Q8J112|ETFA_CRYNV Probable electron transfer flavoprotei... 137 3e-32
sp|P53573|ETFA_BRAJA Electron transfer flavoprotein alpha-s... 137 3e-32
sp|Q5Y223|ETFA_CRYGA Probable electron transfer flavoprotei... 130 4e-30
>sp|Q8HXY0|ETFA_MACFA Electron transfer flavoprotein alpha-subunit, mitochondrial
precursor (Alpha-ETF)
Length = 333
Score = 176 bits (445), Expect = 9e-44
Identities = 89/217 (41%), Positives = 139/217 (64%)
Frame = +3
Query: 21 MIKSVVFNSFIRSSIKIRLASSLVIADHSNGKLNPITLNVVTAASKLGNDIHALVIGENC 200
M ++ R++ +R S+LVIA+H+N L PITLN +TAA++LG ++ LV G C
Sbjct: 1 MFRAAAPGQLRRAASSLRFQSTLVIAEHANDSLAPITLNTITAATRLGGEVSCLVAGTKC 60
Query: 201 KSVAEEVSKIKGVKVVHLADNAIFKGQLAESISSLLEKLQGKQNFSHILTGATSTGKNFM 380
VA+++ K+ G+ V +A + ++G LAE ++ L+ Q + N++HI GA++ GKN +
Sbjct: 61 DKVAQDLCKVAGIAKVLVAQHDAYRGLLAEDLTPLILATQKQFNYTHICAGASAFGKNLL 120
Query: 381 PRFASKSGISVVSDITGIQSPNTFVRTIYAGNAIQTVQSDEKVHCITVRGTSFEPAVLEN 560
PR A+K ++ +SDI I+SP+TFVRTIYAGNA+ TV+ DEKV +VRGTSFE A
Sbjct: 121 PRVAAKLEVAPISDIIAIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFEAAATSG 180
Query: 561 NAVEIKSVPEAAEIKNDKSKFIKQEILKNDRPELASA 671
+ + + + S+++ Q++ K+DRPEL A
Sbjct: 181 GTASSEKASSTSPV--EISEWLDQKLTKSDRPELTGA 215
Score = 48.1 bits (113), Expect = 3e-05
Identities = 20/26 (76%), Positives = 26/26 (100%)
Frame = +1
Query: 676 IVISGGRGLKSGENFKILYELADKLN 753
+V+SGGRGLKSGENFK+LY+LAD+L+
Sbjct: 217 VVVSGGRGLKSGENFKLLYDLADQLH 242
>sp|P13804|ETFA_HUMAN Electron transfer flavoprotein alpha-subunit, mitochondrial
precursor (Alpha-ETF)
Length = 333
Score = 175 bits (443), Expect = 1e-43
Identities = 88/217 (40%), Positives = 140/217 (64%)
Frame = +3
Query: 21 MIKSVVFNSFIRSSIKIRLASSLVIADHSNGKLNPITLNVVTAASKLGNDIHALVIGENC 200
M ++ R++ +R S+LVIA+H+N L PITLN +TAA++LG ++ LV G C
Sbjct: 1 MFRAAAPGQLRRAASLLRFQSTLVIAEHANDSLAPITLNTITAATRLGGEVSCLVAGTKC 60
Query: 201 KSVAEEVSKIKGVKVVHLADNAIFKGQLAESISSLLEKLQGKQNFSHILTGATSTGKNFM 380
VA+++ K+ G+ V +A + ++KG L E ++ L+ Q + N++HI GA++ GKN +
Sbjct: 61 DKVAQDLCKVAGIAKVLVAQHDVYKGLLPEELTPLILATQKQFNYTHICAGASAFGKNLL 120
Query: 381 PRFASKSGISVVSDITGIQSPNTFVRTIYAGNAIQTVQSDEKVHCITVRGTSFEPAVLEN 560
PR A+K ++ +SDI I+SP+TFVRTIYAGNA+ TV+ DEKV +VRGTSF+ A
Sbjct: 121 PRVAAKLEVAPISDIIAIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFDAAATSG 180
Query: 561 NAVEIKSVPEAAEIKNDKSKFIKQEILKNDRPELASA 671
+ + + + + S+++ Q++ K+DRPEL A
Sbjct: 181 GSASSEKASSTSPV--EISEWLDQKLTKSDRPELTGA 215
Score = 48.1 bits (113), Expect = 3e-05
Identities = 20/26 (76%), Positives = 26/26 (100%)
Frame = +1
Query: 676 IVISGGRGLKSGENFKILYELADKLN 753
+V+SGGRGLKSGENFK+LY+LAD+L+
Sbjct: 217 VVVSGGRGLKSGENFKLLYDLADQLH 242
>sp|Q99LC5|ETFA_MOUSE Electron transfer flavoprotein alpha-subunit, mitochondrial
precursor (Alpha-ETF)
Length = 333
Score = 175 bits (443), Expect = 1e-43
Identities = 90/217 (41%), Positives = 139/217 (64%)
Frame = +3
Query: 21 MIKSVVFNSFIRSSIKIRLASSLVIADHSNGKLNPITLNVVTAASKLGNDIHALVIGENC 200
M ++ R++ +R S+LVIA+H+N L PITLN +TAA +LG ++ LV G C
Sbjct: 1 MFRAAAPGQLRRAASLLRFQSTLVIAEHANDSLAPITLNTITAAGRLGGEVSCLVAGTKC 60
Query: 201 KSVAEEVSKIKGVKVVHLADNAIFKGQLAESISSLLEKLQGKQNFSHILTGATSTGKNFM 380
V +++ K+ GV V +A + +KG L E ++ L+ + Q + +++HI GA++ GKN +
Sbjct: 61 DKVVQDLCKVAGVAKVLVAQHDAYKGLLPEELTPLILETQKQFSYTHICAGASAFGKNLL 120
Query: 381 PRFASKSGISVVSDITGIQSPNTFVRTIYAGNAIQTVQSDEKVHCITVRGTSFEPAVLEN 560
PR A+K ++ VSDI I+SP+TFVRTIYAGNA+ TV+ DEKV +VRGTSFE A
Sbjct: 121 PRVAAKLNVAPVSDIIEIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFEAAATSG 180
Query: 561 NAVEIKSVPEAAEIKNDKSKFIKQEILKNDRPELASA 671
+ + P ++ + S+++ Q++ K+DRPEL A
Sbjct: 181 GSASSEKAPSSSSV--GISEWLDQKLTKSDRPELTGA 215
Score = 48.1 bits (113), Expect = 3e-05
Identities = 20/26 (76%), Positives = 26/26 (100%)
Frame = +1
Query: 676 IVISGGRGLKSGENFKILYELADKLN 753
+V+SGGRGLKSGENFK+LY+LAD+L+
Sbjct: 217 VVVSGGRGLKSGENFKLLYDLADQLH 242
>sp|Q5RC31|ETFA_PONPY Electron transfer flavoprotein alpha-subunit, mitochondrial
precursor (Alpha-ETF)
Length = 333
Score = 173 bits (438), Expect = 6e-43
Identities = 87/217 (40%), Positives = 140/217 (64%)
Frame = +3
Query: 21 MIKSVVFNSFIRSSIKIRLASSLVIADHSNGKLNPITLNVVTAASKLGNDIHALVIGENC 200
M ++ R++ +R S+LVIA+H++ L PITLN +TAA++LG ++ LV G C
Sbjct: 1 MFRAAAPGQLRRAASLLRFQSTLVIAEHADDSLAPITLNTITAATRLGGEVSCLVAGTKC 60
Query: 201 KSVAEEVSKIKGVKVVHLADNAIFKGQLAESISSLLEKLQGKQNFSHILTGATSTGKNFM 380
VA+++ K+ G+ V +A + +++G L E ++ L+ Q + N++HI GA++ GKN +
Sbjct: 61 DKVAQDLCKVAGIAKVLVAQHDVYRGLLPEELTPLILATQKQFNYTHICAGASAFGKNLL 120
Query: 381 PRFASKSGISVVSDITGIQSPNTFVRTIYAGNAIQTVQSDEKVHCITVRGTSFEPAVLEN 560
PR A+K ++ +SDI I+SP+TFVRTIYAGNA+ TV+ DEKV +VRGTSFE A
Sbjct: 121 PRVAAKLEVAPISDIIAIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFEAAATSG 180
Query: 561 NAVEIKSVPEAAEIKNDKSKFIKQEILKNDRPELASA 671
+ + + + + S+++ Q++ K+DRPEL A
Sbjct: 181 GSASSEKASSTSPV--EISEWLDQKLTKSDRPELTGA 215
Score = 46.2 bits (108), Expect = 1e-04
Identities = 19/24 (79%), Positives = 24/24 (100%)
Frame = +1
Query: 676 IVISGGRGLKSGENFKILYELADK 747
+V+SGGRGLKSGENFK+LY+LAD+
Sbjct: 217 VVVSGGRGLKSGENFKLLYDLADQ 240
>sp|P13803|ETFA_RAT Electron transfer flavoprotein alpha-subunit, mitochondrial
precursor (Alpha-ETF)
Length = 333
Score = 172 bits (435), Expect = 1e-42
Identities = 89/217 (41%), Positives = 138/217 (63%)
Frame = +3
Query: 21 MIKSVVFNSFIRSSIKIRLASSLVIADHSNGKLNPITLNVVTAASKLGNDIHALVIGENC 200
M ++ R++ +R S+LVIA+H+N L PITLN +TAA +LG ++ LV G C
Sbjct: 1 MFRAAAPGQLRRAASLLRFQSTLVIAEHANDSLAPITLNTITAAGRLGGEVSCLVAGTKC 60
Query: 201 KSVAEEVSKIKGVKVVHLADNAIFKGQLAESISSLLEKLQGKQNFSHILTGATSTGKNFM 380
V +++ K+ GV V +A + +KG L E ++ L+ + Q + +++HI+ GA++ GKN +
Sbjct: 61 DKVVQDLCKVAGVAKVLVAQHDAYKGLLPEELTPLILETQKQFSYTHIVAGASAFGKNLL 120
Query: 381 PRFASKSGISVVSDITGIQSPNTFVRTIYAGNAIQTVQSDEKVHCITVRGTSFEPAVLEN 560
PR A+K ++ VSDI I+SP+TFVRTIYA NA+ TV+ DEKV +VRGTSFE A
Sbjct: 121 PRVAAKLNVAPVSDIIEIKSPDTFVRTIYAANALCTVKCDEKVKVFSVRGTSFEAAAASG 180
Query: 561 NAVEIKSVPEAAEIKNDKSKFIKQEILKNDRPELASA 671
+ + P ++ S+++ Q++ K+DRPEL A
Sbjct: 181 GSASSEKAPSSSSA--GISEWLDQKLTKSDRPELTGA 215
Score = 45.8 bits (107), Expect = 1e-04
Identities = 19/26 (73%), Positives = 25/26 (96%)
Frame = +1
Query: 676 IVISGGRGLKSGENFKILYELADKLN 753
+V+SGGRGLKSGENFK+ Y+LAD+L+
Sbjct: 217 VVVSGGRGLKSGENFKLQYDLADQLH 242
>sp|Q93615|ETFA_CAEEL Probable electron transfer flavoprotein alpha-subunit,
mitochondrial precursor (Alpha-ETF)
Length = 332
Score = 171 bits (432), Expect = 3e-42
Identities = 89/200 (44%), Positives = 137/200 (68%)
Frame = +3
Query: 72 RLASSLVIADHSNGKLNPITLNVVTAASKLGNDIHALVIGENCKSVAEEVSKIKGVKVVH 251
RL S+LV+A+H KL PITLN +TAASKLGN++ LV G N VAE+V+K+ GVK V
Sbjct: 19 RLNSTLVVAEHDETKLAPITLNAITAASKLGNEVSVLVTGANATKVAEQVAKVNGVKRVL 78
Query: 252 LADNAIFKGQLAESISSLLEKLQGKQNFSHILTGATSTGKNFMPRFASKSGISVVSDITG 431
+A + K L E ++ ++ Q + NF+ I G+++ G+ +PR A+K +S +SD+T
Sbjct: 79 VAQDEKLKNNLPERVAPVILASQKQFNFTAITAGSSAFGRGVIPRVAAKLDVSSISDVTE 138
Query: 432 IQSPNTFVRTIYAGNAIQTVQSDEKVHCITVRGTSFEPAVLENNAVEIKSVPEAAEIKND 611
+ S ++F RT+YAGNA++ V+S + +T RGTSFEPA E + +++ P +A+IK D
Sbjct: 139 VHSADSFTRTLYAGNAVKKVKSTAPIKLLTFRGTSFEPA-KEGGSGAVENAP-SADIKTD 196
Query: 612 KSKFIKQEILKNDRPELASA 671
S+F+ QE+ K++RP+LA+A
Sbjct: 197 LSEFLGQELSKSERPDLATA 216
Score = 47.4 bits (111), Expect = 5e-05
Identities = 19/25 (76%), Positives = 25/25 (100%)
Frame = +1
Query: 676 IVISGGRGLKSGENFKILYELADKL 750
+V+SGGRGLKSG+NFK++Y+LADKL
Sbjct: 218 VVVSGGRGLKSGDNFKLIYDLADKL 242
>sp|Q8J0W1|ETFA_CRYNE Probable electron transfer flavoprotein alpha-subunit,
mitochondrial precursor (Alpha-ETF)
Length = 346
Score = 137 bits (346), Expect = 3e-32
Identities = 78/210 (37%), Positives = 123/210 (58%), Gaps = 5/210 (2%)
Frame = +3
Query: 57 SSIKIRLASSLVIADHSNGKLNPITLNVVTAASKLGNDIHALVIG--ENCKSVAEEVSKI 230
+S RL SSLV +H GKLN +L+ VTAA LGND H L++G ++V + +I
Sbjct: 18 ASTNSRLVSSLVFLEHKAGKLNEASLSAVTAAKTLGNDTHGLLVGTKSEIENVLDRTKEI 77
Query: 231 KGVKVVHLADNAIFKGQLAESISSLLEKLQGKQNFSHILTGATSTGKNFMPRFASKSGIS 410
K + ++LA + + LAE ++SLL + ++ SHI T+ GKN PR A S
Sbjct: 78 KDLSKIYLATSDTYSHSLAEPLASLLASIASAKDVSHIFAAHTAVGKNIFPRLAGLLDTS 137
Query: 411 VVSDITGIQSP-NTFVRTIYAGNAIQTVQSDEK--VHCITVRGTSFEPAVLENNAVEIKS 581
+++DI ++S +TF R IYAGNA+ T++S K V +TVR T+F+ A + N + ++
Sbjct: 138 LIADIIALESSGDTFTRPIYAGNAVLTIKSSPKDSVKVVTVRSTAFDKAAVANGSAAVED 197
Query: 582 VPEAAEIKNDKSKFIKQEILKNDRPELASA 671
V + ++F+ +E+ + RP+LASA
Sbjct: 198 VDIIT--VDTPTQFVSEELTVSSRPDLASA 225
>sp|Q8J112|ETFA_CRYNV Probable electron transfer flavoprotein alpha-subunit,
mitochondrial precursor (Alpha-ETF)
Length = 346
Score = 137 bits (345), Expect = 3e-32
Identities = 80/210 (38%), Positives = 127/210 (60%), Gaps = 5/210 (2%)
Frame = +3
Query: 57 SSIKIRLASSLVIADHSNGKLNPITLNVVTAASKLGNDIHALVIGE--NCKSVAEEVSKI 230
+S + RLASSLV+ +H G+LN +L+ VTAA GND H +++G + V +E KI
Sbjct: 18 ASTRSRLASSLVLLEHKAGRLNDASLSAVTAAKATGNDTHGILVGSMAEVEGVLKEAKKI 77
Query: 231 KGVKVVHLADNAIFKGQLAESISSLLEKLQGKQNFSHILTGATSTGKNFMPRFASKSGIS 410
K + ++LA + + LAE+++ LL + ++ SH+ T+ GKN PR A S
Sbjct: 78 KDLSKIYLATSDAYSHSLAEALAPLLASIVPTKDVSHVFAAHTAVGKNVFPRLAGMLDSS 137
Query: 411 VVSDITGIQ-SPNTFVRTIYAGNAIQTVQSDEK--VHCITVRGTSFEPAVLENNAVEIKS 581
+V+DI + S TF R IYAGNA+ TV+S K V +TVR T+F+ A + + + +++
Sbjct: 138 LVADIIALDPSRGTFTRPIYAGNAVLTVKSSPKDSVKIVTVRSTAFDKAAIADGSADVED 197
Query: 582 VPEAAEIKNDKSKFIKQEILKNDRPELASA 671
V E I++ ++FI +E+ + RP+LASA
Sbjct: 198 V-EILAIES-PTQFISEELTVSSRPDLASA 225
Score = 30.0 bits (66), Expect = 7.5
Identities = 17/25 (68%), Positives = 19/25 (76%), Gaps = 1/25 (4%)
Frame = +1
Query: 679 VISGGRGLKSGENF-KILYELADKL 750
V+SGGR LKS E+F KIL LAD L
Sbjct: 228 VVSGGRALKSKESFDKILDPLADSL 252
>sp|P53573|ETFA_BRAJA Electron transfer flavoprotein alpha-subunit (Alpha-ETF) (Electron
transfer flavoprotein large subunit) (ETFLS)
Length = 314
Score = 137 bits (345), Expect = 3e-32
Identities = 82/197 (41%), Positives = 121/197 (61%)
Frame = +3
Query: 81 SSLVIADHSNGKLNPITLNVVTAASKLGNDIHALVIGENCKSVAEEVSKIKGVKVVHLAD 260
++L+IA+H N L T +TAA+ LG D+ LV GE K+ A+ +K+ GVK V LAD
Sbjct: 2 TTLLIAEHDNASLKDATNKALTAAAALGADVEVLVAGEGAKAAADAAAKLAGVKKVLLAD 61
Query: 261 NAIFKGQLAESISSLLEKLQGKQNFSHILTGATSTGKNFMPRFASKSGISVVSDITGIQS 440
A++ LAE +++L+ L ++ I+ ATS KN MPR A+ + VS+I + +
Sbjct: 62 GALYAHDLAEPLAALIVSL--APSYDAIVAPATSRFKNVMPRVAALLDVMQVSEIIKVVA 119
Query: 441 PNTFVRTIYAGNAIQTVQSDEKVHCITVRGTSFEPAVLENNAVEIKSVPEAAEIKNDKSK 620
P+T+ R IYAGNAIQTV+S + ITVR ++F A E + ++SV AA+ S
Sbjct: 120 PDTYERPIYAGNAIQTVKSKDAKKVITVRTSTF-AAAGEGGSAPVESVQAAAD--PGLSS 176
Query: 621 FIKQEILKNDRPELASA 671
F+ +E+ K+DRPEL SA
Sbjct: 177 FVGEEVAKSDRPELTSA 193
Score = 30.4 bits (67), Expect = 5.8
Identities = 15/26 (57%), Positives = 20/26 (76%), Gaps = 1/26 (3%)
Frame = +1
Query: 676 IVISGGRGLKSGENF-KILYELADKL 750
I++SGGR ++S ENF K + LADKL
Sbjct: 195 IIVSGGRAMQSRENFAKYIEPLADKL 220
>sp|Q5Y223|ETFA_CRYGA Probable electron transfer flavoprotein alpha-subunit,
mitochondrial precursor (Alpha-ETF)
Length = 346
Score = 130 bits (327), Expect = 4e-30
Identities = 75/205 (36%), Positives = 121/205 (59%), Gaps = 5/205 (2%)
Frame = +3
Query: 72 RLASSLVIADHSNGKLNPITLNVVTAASKLGNDIHALVIG--ENCKSVAEEVSKIKGVKV 245
RL SSLV +H GKL+ +L+ VTAA LGND H L++G ++ + +IKG+
Sbjct: 23 RLVSSLVFLEHKAGKLSEASLSAVTAAKTLGNDTHGLLVGTKSEIENALDRAKEIKGLSK 82
Query: 246 VHLADNAIFKGQLAESISSLLEKLQGKQNFSHILTGATSTGKNFMPRFASKSGISVVSDI 425
++LA + + LAE+++SLL + ++ SH+ T+ GKN PR A S+++DI
Sbjct: 83 IYLARSDSYSHSLAEALASLLASIVSAKDVSHLFAAHTAVGKNVFPRLAGLLDSSLIADI 142
Query: 426 TGIQSP-NTFVRTIYAGNAIQTVQSDEK--VHCITVRGTSFEPAVLENNAVEIKSVPEAA 596
++S +TF R IYAGNA+ T++S K V +TVR T+F+ A + + I+ V
Sbjct: 143 IALESSGDTFTRPIYAGNAVLTIKSSPKDSVKIVTVRSTAFDKAPVAAGSAVIEDVDIIT 202
Query: 597 EIKNDKSKFIKQEILKNDRPELASA 671
+ ++F+ +E+ + RP+LASA
Sbjct: 203 --VDTPTQFVSEELTVSSRPDLASA 225
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 77,272,958
Number of Sequences: 369166
Number of extensions: 1487184
Number of successful extensions: 4288
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4153
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4261
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7454154400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)