Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_022_A06
(572 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O14880|MGST3_HUMAN Microsomal glutathione S-transferase ... 89 1e-17
sp|Q9CPU4|MGST3_MOUSE Microsomal glutathione S-transferase ... 89 1e-17
sp|P73795|Y1147_SYNY3 Hypothetical protein sll1147 56 7e-08
sp|Q99735|MGST2_HUMAN Microsomal glutathione S-transferase ... 55 1e-07
sp|Q16873|LTC4S_HUMAN Leukotriene C4 synthase (Leukotriene-... 37 0.035
sp|P55493|Y4IJ_RHISN Hypothetical 65.5 kDa protein y4iJ 30 4.3
sp|O94477|MYO52_SCHPO Myosin-52 (Myosin type V-2) 30 5.6
>sp|O14880|MGST3_HUMAN Microsomal glutathione S-transferase 3 (Microsomal GST-3)
(Microsomal GST-III)
Length = 152
Score = 88.6 bits (218), Expect = 1e-17
Identities = 56/135 (41%), Positives = 76/135 (56%), Gaps = 3/135 (2%)
Frame = +1
Query: 103 YGGVLMILAETYLLNFYLMYKVAKARKQYDVKCPAMYSDTSEE---FNCIQRSHQNYLEN 273
YG VL+ A ++++ +L V+KARK+Y V+ P MYS E FNCIQR+HQN LE
Sbjct: 8 YGFVLLTGAASFIMVAHLAINVSKARKKYKVEYPIMYSTDPENGHIFNCIQRAHQNTLEV 67
Query: 274 ISFFIPFLLVSGLEFPRFTILCGKAFLIGRVLFDRGYTAEDPNKRKLGFKISLFGGLMPL 453
F+ FL V G+ PR G A+++GRVL+ GY +P+KR G G + L
Sbjct: 68 YPPFLFFLAVGGVYHPRIASGLGLAWIVGRVLYAYGYYTGEPSKRSRGAL-----GSIAL 122
Query: 454 VGAVGVMAVGKCFLH 498
+G VG V F H
Sbjct: 123 LGLVGT-TVCSAFQH 136
>sp|Q9CPU4|MGST3_MOUSE Microsomal glutathione S-transferase 3 (Microsomal GST-3)
(Microsomal GST-III)
Length = 153
Score = 88.6 bits (218), Expect = 1e-17
Identities = 55/122 (45%), Positives = 71/122 (58%), Gaps = 3/122 (2%)
Frame = +1
Query: 103 YGGVLMILAETYLLNFYLMYKVAKARKQYDVKCPAMYSDTSEE---FNCIQRSHQNYLEN 273
YG VL+ A ++++ +L V KARK+Y V+ P MYS E FNCIQR+HQN LE
Sbjct: 8 YGFVLLTGAASFVMVLHLAINVGKARKKYKVEYPVMYSTDPENGHMFNCIQRAHQNTLEV 67
Query: 274 ISFFIPFLLVSGLEFPRFTILCGKAFLIGRVLFDRGYTAEDPNKRKLGFKISLFGGLMPL 453
F+ FL V G+ PR G A++IGRVL+ GY DP+KR G SL L L
Sbjct: 68 YPPFLFFLTVGGVYHPRIASGLGLAWIIGRVLYAYGYYTGDPSKRYRGAVGSL--ALFAL 125
Query: 454 VG 459
+G
Sbjct: 126 MG 127
>sp|P73795|Y1147_SYNY3 Hypothetical protein sll1147
Length = 137
Score = 55.8 bits (133), Expect = 7e-08
Identities = 37/117 (31%), Positives = 62/117 (52%), Gaps = 1/117 (0%)
Frame = +1
Query: 118 MILAETYLLNFYLMYKVAKARKQYDVKCPAMYSDTSEEFNCIQRSHQNYLENISFFIPFL 297
+I A +L L+ V +AR +Y V PA + +E+F + R N LE ++FF+P L
Sbjct: 11 LITALATMLYLVLVINVGRARAKYGVMPPA--TTGNEDFERVLRVQYNTLEQLAFFLPGL 68
Query: 298 -LVSGLEFPRFTILCGKAFLIGRVLFDRGYTAEDPNKRKLGFKISLFGGLMPLVGAV 465
L + P + G +L+GR+L+ GY + KR +GF + ++ +VGA+
Sbjct: 69 WLFAIYRDPTIAAILGAVWLLGRILYAWGY-YQAAEKRMVGFALGSLSSMILVVGAL 124
>sp|Q99735|MGST2_HUMAN Microsomal glutathione S-transferase 2 (Microsomal GST-2)
(Microsomal GST-II)
Length = 147
Score = 55.1 bits (131), Expect = 1e-07
Identities = 40/116 (34%), Positives = 61/116 (52%), Gaps = 1/116 (0%)
Frame = +1
Query: 151 YLMYKVAKARKQYDVKCPAMYSDTSEEFNCIQRSHQNYLENISFFIPFLLVSGLEFPRFT 330
Y +V KAR +Y V PA+ S EF + R+ QN +E FI L ++G F +
Sbjct: 21 YFALQVGKARLKYKVTPPAVTG--SPEFERVFRAQQNCVEFYPIFIITLWMAGWYFNQVF 78
Query: 331 ILC-GKAFLIGRVLFDRGYTAEDPNKRKLGFKISLFGGLMPLVGAVGVMAVGKCFL 495
C G ++ GR L+ GY +E KR GF++SL G++ L+ +G + + FL
Sbjct: 79 ATCLGLVYIYGRHLYFWGY-SEAAKKRITGFRLSL--GILALLTLLGALGIANSFL 131
>sp|Q16873|LTC4S_HUMAN Leukotriene C4 synthase (Leukotriene-C(4) synthase) (LTC4 synthase)
Length = 150
Score = 37.0 bits (84), Expect = 0.035
Identities = 30/102 (29%), Positives = 47/102 (46%), Gaps = 1/102 (0%)
Frame = +1
Query: 82 NEVAMLGYGGVLMILAETYLLNFYLMYKVAKARKQYDVKCPAMYSDTSEEFNCIQRSHQN 261
+EVA+L +L +L L Y +V AR+ + V P + EF + R+ N
Sbjct: 3 DEVALLAAVTLLGVL-----LQAYFSLQVISARRAFRVSPPL--TTGPPEFERVYRAQVN 55
Query: 262 YLENISFFIPFLLVSGLEFPR-FTILCGKAFLIGRVLFDRGY 384
E F+ L V+G+ F LCG +L R+ + +GY
Sbjct: 56 CSEYFPLFLATLWVAGIFFHEGAAALCGLVYLFARLRYFQGY 97
>sp|P55493|Y4IJ_RHISN Hypothetical 65.5 kDa protein y4iJ
Length = 596
Score = 30.0 bits (66), Expect = 4.3
Identities = 15/49 (30%), Positives = 25/49 (51%)
Frame = +1
Query: 274 ISFFIPFLLVSGLEFPRFTILCGKAFLIGRVLFDRGYTAEDPNKRKLGF 420
++F +P+ ++ LE P FT+ G FL L G+ D + +GF
Sbjct: 65 VTFSMPYEWLAALEQPTFTLTAGPPFLSSDYLDRFGFITADSSGLPVGF 113
>sp|O94477|MYO52_SCHPO Myosin-52 (Myosin type V-2)
Length = 1516
Score = 29.6 bits (65), Expect = 5.6
Identities = 16/64 (25%), Positives = 32/64 (50%), Gaps = 3/64 (4%)
Frame = +1
Query: 79 HNEVAMLGYGGVLMILAETY--LLNFYLMYKV-AKARKQYDVKCPAMYSDTSEEFNCIQR 249
+N++ + Y G+++I + L N Y V A + K D P +Y+ + + C+ +
Sbjct: 97 YNQLQIYTYSGIVLIAVNPFQRLPNLYTHEIVRAYSEKSRDELDPHLYAIAEDSYKCMNQ 156
Query: 250 SHQN 261
H+N
Sbjct: 157 EHKN 160
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 63,300,862
Number of Sequences: 369166
Number of extensions: 1208887
Number of successful extensions: 3012
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 2959
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3009
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 4226258440
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)