Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_021_P13
(767 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P52826|CACP_COLLI Carnitine O-acetyltransferase precurso... 33 0.85
sp|O74552|VPS35_SCHPO Vacuolar protein sorting-associated p... 33 0.85
sp|Q9Z6W0|SYG_CHLPN Glycyl-tRNA synthetase (GlyRS) [Include... 32 1.5
sp|P48616|VIME_BOVIN Vimentin 31 4.2
sp|Q6MT07|DNAJ_MYCMS Chaperone protein dnaJ 31 4.2
sp|Q27171|DYHC_PARTE Dynein heavy chain, cytosolic (DYHC) (... 31 4.2
sp|Q92359|YDHE_SCHPO Hypothetical protein C6G9.14 in chromo... 30 5.5
sp|Q9CFZ0|SBCC_LACLA Nuclease sbcCD subunit C 30 5.5
sp|O12946|P53_PLAFE Cellular tumor antigen p53 (Tumor suppr... 30 7.2
sp|Q92SW4|IF2_RHIME Translation initiation factor IF-2 30 9.4
>sp|P52826|CACP_COLLI Carnitine O-acetyltransferase precursor (Carnitine acetylase) (CAT)
(Carnitine acetyltransferase) (CrAT)
Length = 627
Score = 33.1 bits (74), Expect = 0.85
Identities = 35/151 (23%), Positives = 71/151 (47%), Gaps = 4/151 (2%)
Frame = +2
Query: 239 LDLIIKCYNTCQQAFKKNYPKLNFAIEQCIEPNRRYVVLSETSIYFKVCASPARDCKSMS 418
LD+I+ ++ F KNYPK + I P+ ++ L+ Y+++ +S S
Sbjct: 408 LDVIVLVFHQ----FGKNYPK-----SEKISPDA-FIQLALQLAYYRMYGHSCATYESAS 457
Query: 419 LRKRPIKQEMVERPQSINSNEFVEIKGKRFFPILVDEDMLDIVKSVNKYANKDDPEIKAR 598
LR + + R SI S++FV+ P D++ D+++ + A+K+ + +
Sbjct: 458 LRMFRLGRTDTIRSTSIESHKFVQSMDS---PDKSDQEKADLLRRATQ-AHKEYTNMAIQ 513
Query: 599 IEILQHNF----IQSIQNLVSDpreNIENQY 679
+ + +Q+I++LVS P ++ Y
Sbjct: 514 GNAIDRHLLGLKLQAIEDLVSIPELFMDTAY 544
>sp|O74552|VPS35_SCHPO Vacuolar protein sorting-associated protein vps35
Length = 785
Score = 33.1 bits (74), Expect = 0.85
Identities = 50/205 (24%), Positives = 94/205 (45%), Gaps = 29/205 (14%)
Frame = +2
Query: 116 EYASKSILEIHDTDAIYEAIELPRNEEQISVLVKYTRPDVILDLIIKCYNTCQQAFKK-- 289
EYA + +L H + I LP + +S+L IL+ ++CY Q +
Sbjct: 306 EYAIQEVLWSHVVEVIQSRSGLPL-DCIVSILSS------ILNFFLRCYPYKPQYADRVF 358
Query: 290 --------NYPKLNFAI-EQCIEPNRRYVVLSETSIY--FKVCAS-----PARDCKSMSL 421
N P L A+ E+ ++ + ++L + + F C P + + +L
Sbjct: 359 QYINEHIINQPSLRSALHERPLQKSLCAILLLPLTYFPSFSYCLELQNFLPVFNAQDPNL 418
Query: 422 R---KRPIKQEMVERPQSINS-NEFVEIKGKRFFPILVDE---DMLDIVKSVN---KYAN 571
R R I Q+++E+ S++ E E+ G F +++++ D LD +++V Y N
Sbjct: 419 RYDIARMIVQKIIEKGHSLSELTEAQELLG--FVSVIIEKKGVDSLDDLQNVALMVHYLN 476
Query: 572 KDDPEIKARI-EILQHNFIQSIQNL 643
DDP+I+ I L+ FI++ +N+
Sbjct: 477 NDDPQIQIEILRSLKDTFIKAGENV 501
>sp|Q9Z6W0|SYG_CHLPN Glycyl-tRNA synthetase (GlyRS) [Includes: Glycyl-tRNA synthetase
alpha chain (Glycine--tRNA ligase alpha chain);
Glycyl-tRNA synthetase beta chain (Glycine--tRNA ligase
beta chain)]
Length = 1010
Score = 32.3 bits (72), Expect = 1.5
Identities = 20/67 (29%), Positives = 36/67 (53%)
Frame = +2
Query: 104 IDQLEYASKSILEIHDTDAIYEAIELPRNEEQISVLVKYTRPDVILDLIIKCYNTCQQAF 283
I QLE ++ +L H+ +YE +E+ + ++++LVK P+V+ Q+AF
Sbjct: 335 IQQLESLARQVLTDHNI--VYEGLEVLGSPRRLALLVKNVAPEVV-----------QKAF 381
Query: 284 KKNYPKL 304
+K P L
Sbjct: 382 EKKGPML 388
>sp|P48616|VIME_BOVIN Vimentin
Length = 466
Score = 30.8 bits (68), Expect = 4.2
Identities = 23/85 (27%), Positives = 41/85 (48%), Gaps = 2/85 (2%)
Frame = +2
Query: 437 KQEMVERPQSINSNEFVEIKG--KRFFPILVDEDMLDIVKSVNKYANKDDPEIKARIEIL 610
K +E+ I E ++KG K L +E+M ++ + V++ N KAR E+
Sbjct: 120 KVRFLEQQNKILLAELEQLKGQGKSRLGQLYEEEMRELRRQVDQLTND-----KARAEVE 174
Query: 611 QHNFIQSIQNLVSDpreNIENQYIQ 685
+HN + I L RE ++ + +Q
Sbjct: 175 RHNLAEDIMRL----REKLQEEMLQ 195
>sp|Q6MT07|DNAJ_MYCMS Chaperone protein dnaJ
Length = 372
Score = 30.8 bits (68), Expect = 4.2
Identities = 18/52 (34%), Positives = 28/52 (53%), Gaps = 4/52 (7%)
Frame = +2
Query: 458 PQSINSNEFVEIKGKRFFPILVDEDMLDIVKSVN----KYANKDDPEIKARI 601
P+SINSNEF+ I K + + + D++ VN K NK + E+ +I
Sbjct: 307 PKSINSNEFIIINNKGLYKSINKDKRGDLIIKVNIVVPKNLNKKEKELIEQI 358
>sp|Q27171|DYHC_PARTE Dynein heavy chain, cytosolic (DYHC) (DHC08)
Length = 4540
Score = 30.8 bits (68), Expect = 4.2
Identities = 26/100 (26%), Positives = 44/100 (44%), Gaps = 13/100 (13%)
Frame = +2
Query: 434 IKQEMVER---PQSINSNE--FVEIKGKRFFPILVDEDMLDIVKSVNKYANKDDPE---I 589
I Q +VE+ QS N N F ++GK + LD ++ + + + PE +
Sbjct: 4165 ILQSLVEQFFTEQSFNHNHPLFFTLEGKEAITVPEGRTYLDFMQWIEQLPKTESPEWSGL 4224
Query: 590 KARIEILQHN-----FIQSIQNLVSDpreNIENQYIQTPK 694
+ +E +Q + I +QNL + E I +QT K
Sbjct: 4225 PSNVERVQRDQLTQKLITKVQNLQQEGEEEITQIEVQTEK 4264
>sp|Q92359|YDHE_SCHPO Hypothetical protein C6G9.14 in chromosome I
Length = 681
Score = 30.4 bits (67), Expect = 5.5
Identities = 18/53 (33%), Positives = 28/53 (52%), Gaps = 8/53 (15%)
Frame = +2
Query: 143 IHDTDAIYEAIELPRNEEQISVLVKYTRPDVIL--------DLIIKCYNTCQQ 277
+H T A+ + I+L + +QIS +V RP+V+L +I KC N Q
Sbjct: 427 MHGTRALQKIIDLVSSPDQISCIVNALRPNVVLLTKDLNGNHVIQKCLNKFSQ 479
>sp|Q9CFZ0|SBCC_LACLA Nuclease sbcCD subunit C
Length = 1046
Score = 30.4 bits (67), Expect = 5.5
Identities = 30/127 (23%), Positives = 63/127 (49%), Gaps = 4/127 (3%)
Frame = +2
Query: 284 KKNYPKLNFAIEQCIEPNRRYVVLSETSIYFKVCASPARDCKSMSLRKRPIKQEMVERPQ 463
+ +Y +L +++ E ++ VL E + + + + S+ L+K I+ + E+
Sbjct: 540 ESSYKELGNLLKEIDESQKKQTVLLEKNKQLQQLKTELKT--SLDLKK--IEADEFEKEL 595
Query: 464 SINSNEFV----EIKGKRFFPILVDEDMLDIVKSVNKYANKDDPEIKARIEILQHNFIQS 631
SI +EF+ +I F + +DE +L++ KS+ K+D E K ++ L+ ++
Sbjct: 596 SILYSEFIADYSQIFPDSFDEVSIDESLLNLTKSLELEEVKND-ETKVKLADLESKKLE- 653
Query: 632 IQNLVSD 652
+Q V D
Sbjct: 654 LQEKVKD 660
>sp|O12946|P53_PLAFE Cellular tumor antigen p53 (Tumor suppressor p53)
Length = 366
Score = 30.0 bits (66), Expect = 7.2
Identities = 47/187 (25%), Positives = 72/187 (38%), Gaps = 22/187 (11%)
Frame = +2
Query: 68 CPNHHQTKTKATIDQLEYASKSILEIHDTDAIYEAIELPRNEEQISVLVKYTRPDV---- 235
CP HHQT+ D E+ S I A+Y E P + Q SV V Y P +
Sbjct: 154 CP-HHQTE-----DTAEHRSHLIRLEGSQRALY--FEDPHTKRQ-SVTVPYEPPQLGSET 204
Query: 236 -ILDLIIKCYNTCQQAFKKNYPKLNFAIEQCIEPNRRYVVLSETSIYFKVCASPARDCKS 412
+ L C ++C + +E P+ +VL +VCA P RD K+
Sbjct: 205 TAILLSFMCNSSCMGGMNRRQILTILTLET---PDG--LVLGRRCFEVRVCACPGRDRKT 259
Query: 413 --MSLRKRPIKQEMVERPQSINSN--------------EFVEIKGKRFFPILV-DEDMLD 541
S K P + ++ + SN E + K F +LV + +
Sbjct: 260 DEESSTKTPNGPKQTKKRKQAPSNSAPHTTTVMKSKSSSSAEEEDKEVFTVLVKGRERYE 319
Query: 542 IVKSVNK 562
I+K +N+
Sbjct: 320 IIKKINE 326
>sp|Q92SW4|IF2_RHIME Translation initiation factor IF-2
Length = 889
Score = 29.6 bits (65), Expect = 9.4
Identities = 26/118 (22%), Positives = 48/118 (40%), Gaps = 1/118 (0%)
Frame = +2
Query: 326 IEPNRRYVVLSETSIYFKVCASPARDCKSMSLRKRPIKQEMVERPQSINSNEFVEIKGKR 505
+E N + + +T + A AR ++ + + + PQ+I S + G
Sbjct: 431 VEQNGQKITFIDTPGHAAFTAMRARGAQATDIAVLVVAADDSVMPQTIESINHAKAAG-- 488
Query: 506 FFPILVDEDMLDIVKSVNKYANKDDPEIKARIEILQHN-FIQSIQNLVSDpreNIENQ 676
+ I+ ++NK K R E+LQH F++S+ V D + +NQ
Sbjct: 489 ----------VPIIVAINKIDKPSANPQKVRTELLQHEVFVESMGGEVLDVEVSAKNQ 536
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 81,806,685
Number of Sequences: 369166
Number of extensions: 1615058
Number of successful extensions: 4228
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4124
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4228
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7115329200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)