Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_021_N13
(610 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 111 1e-24
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 109 6e-24
sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinas... 105 9e-23
sp|Q13153|PAK1_HUMAN Serine/threonine-protein kinase PAK 1 ... 105 9e-23
sp|P35465|PAK1_RAT Serine/threonine-protein kinase PAK 1 (p... 103 3e-22
sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (S... 103 4e-22
sp|O88643|PAK1_MOUSE Serine/threonine-protein kinase PAK 1 ... 103 4e-22
sp|Q62829|PAK3_RAT Serine/threonine-protein kinase PAK 3 (p... 102 6e-22
sp|O75914|PAK3_HUMAN Serine/threonine-protein kinase PAK 3 ... 102 6e-22
sp|Q61036|PAK3_MOUSE Serine/threonine-protein kinase PAK 3 ... 102 6e-22
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 111 bits (278), Expect = 1e-24
Identities = 78/201 (38%), Positives = 111/201 (55%), Gaps = 2/201 (0%)
Frame = +2
Query: 14 SIIR-FSKQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFR 190
S++R +++Q+L L++LH+ I+HRD+KG IL+DN IKL F ++K + ++ T
Sbjct: 172 SVVRTYTRQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTG- 230
Query: 191 NHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLF-FLASFQAIYRL 367
A+S GT W APEVI G+ SADIWS G ++E++TGKAP AI+ +
Sbjct: 231 ---AKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFI 287
Query: 368 GTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFLNSAMEAD*FT*RVDI 547
GT K SDA K F+ CL P RPT +L+KH F+ + T D+
Sbjct: 288 GTTKSHPPIPDTLSSDA-KDFLLKCLQEVPNLRPTASELLKHPFVMGKHKESAST---DL 343
Query: 548 FSWIN*NNTYMLPK*INNCSS 610
S +N N + LP INN S
Sbjct: 344 GSVLN-NLSTPLPLQINNTKS 363
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 109 bits (272), Expect = 6e-24
Identities = 72/201 (35%), Positives = 110/201 (54%), Gaps = 2/201 (0%)
Frame = +2
Query: 14 SIIR-FSKQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFR 190
S++R ++ Q+L L++LH+ I+HRD+KG IL+DN IKL F ++K + ++ +
Sbjct: 171 SVVRTYTNQLLLGLEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATISG- 229
Query: 191 NHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLF-FLASFQAIYRL 367
A+S GT W APEVI G+ SADIWS G ++E++TGKAP AI+ +
Sbjct: 230 ---AKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHI 286
Query: 368 GTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFLNSAMEAD*FT*RVDI 547
GT K P S F+ CL EP RPT +L+KH F+ + + D+
Sbjct: 287 GTTKS-HPPIPDNISSDANDFLLKCLQQEPNLRPTASELLKHPFVTGKQKE---SASKDL 342
Query: 548 FSWIN*NNTYMLPK*INNCSS 610
S+++ N+ LP + N +S
Sbjct: 343 TSFMD-NSCSPLPSELTNITS 362
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
NPK1-related protein kinase 3)
Length = 651
Score = 105 bits (262), Expect = 9e-23
Identities = 63/166 (37%), Positives = 93/166 (56%), Gaps = 1/166 (0%)
Frame = +2
Query: 8 EPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETF 187
EP II ++KQ+L L++LH+ I+HRD+KG IL+DN I+L F ++K + ++
Sbjct: 170 EPVIIMYTKQLLLGLEYLHNNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATV-- 227
Query: 188 RNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLF-FLASFQAIYR 364
+ A+S GT W APEVI G+ SADIWS G ++E+ TGK P F A+
Sbjct: 228 --NGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLH 285
Query: 365 LGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFL 502
+G + K P S K F+ CL EP R + +L++H F+
Sbjct: 286 IG-RTKAHPPIPEDLSPEAKDFLMKCLHKEPSLRLSATELLQHPFV 330
>sp|Q13153|PAK1_HUMAN Serine/threonine-protein kinase PAK 1 (p21-activated kinase 1)
(PAK-1) (P65-PAK) (Alpha-PAK)
Length = 545
Score = 105 bits (262), Expect = 9e-23
Identities = 58/169 (34%), Positives = 90/169 (53%), Gaps = 1/169 (0%)
Frame = +2
Query: 8 EPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGF-MSAKMFPDASSET 184
E I ++ L+AL+FLHS ++HRD+K ILL + +KL F A++ P+ S +
Sbjct: 363 EGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRS 422
Query: 185 FRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYR 364
+ GT W APEV+ + YG DIWS G + +E+I G+ P +A+Y
Sbjct: 423 -------TMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYL 475
Query: 365 LGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFLNSA 511
+ T E P K S + F++ CLD + R + K+L++H+FL A
Sbjct: 476 IATNGTPELQNPEKLSAIFRDFLNRCLDMDVEKRGSAKELLQHQFLKIA 524
>sp|P35465|PAK1_RAT Serine/threonine-protein kinase PAK 1 (p21-activated kinase 1)
(PAK-1) (P68-PAK) (Alpha-PAK) (Protein kinase MUK2)
Length = 544
Score = 103 bits (258), Expect = 3e-22
Identities = 57/169 (33%), Positives = 90/169 (53%), Gaps = 1/169 (0%)
Frame = +2
Query: 8 EPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGF-MSAKMFPDASSET 184
E I ++ L+AL+FLHS ++HRD+K ILL + +KL F A++ P+ S +
Sbjct: 362 EGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRS 421
Query: 185 FRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYR 364
+ GT W APEV+ + YG DIWS G + +E+I G+ P +A+Y
Sbjct: 422 -------TMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYL 474
Query: 365 LGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFLNSA 511
+ T E P K S + F++ CL+ + R + K+L++H+FL A
Sbjct: 475 IATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQHQFLKIA 523
>sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (STE20-like kinase MST3) (MST-3)
(Mammalian STE20-like protein kinase 3)
Length = 443
Score = 103 bits (256), Expect = 4e-22
Identities = 62/167 (37%), Positives = 87/167 (52%), Gaps = 2/167 (1%)
Frame = +2
Query: 8 EPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETF 187
E I ++IL+ LD+LHS+ +HRD+K +LL + ++KL F A D +
Sbjct: 130 ETQIATILREILKGLDYLHSEKKIHRDIKAANVLLSEHGEVKLADFGVAGQLTDTQIK-- 187
Query: 188 RNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRL 367
RN GT W APEVI Y S ADIWS G +EL G+ P L + ++ +
Sbjct: 188 RNTFV----GTPFWMAPEVIKQSAYDSKADIWSLGITAIELARGEPPHSELHPMKVLFLI 243
Query: 368 --GTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFL 502
+E Y S LK FV+ CL+ EP RPT K+L+KH+F+
Sbjct: 244 PKNNPPTLEGNY----SKPLKEFVEACLNKEPSFRPTAKELLKHKFI 286
>sp|O88643|PAK1_MOUSE Serine/threonine-protein kinase PAK 1 (p21-activated kinase 1)
(PAK-1) (P65-PAK) (Alpha-PAK) (CDC42/RAC effector kinase
PAK-A)
Length = 545
Score = 103 bits (256), Expect = 4e-22
Identities = 57/169 (33%), Positives = 89/169 (52%), Gaps = 1/169 (0%)
Frame = +2
Query: 8 EPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGF-MSAKMFPDASSET 184
E I ++ L+AL+FLHS ++HRD+K ILL + +KL F A++ P+ S +
Sbjct: 363 EGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRS 422
Query: 185 FRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYR 364
+ GT W APEV+ + YG DIWS G + +E+I G+ P +A+Y
Sbjct: 423 -------TMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYL 475
Query: 365 LGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFLNSA 511
+ T E P K S + F+ CL+ + R + K+L++H+FL A
Sbjct: 476 IATNGTPELQNPEKLSAIFRDFLQCCLEMDVEKRGSAKELLQHQFLKIA 524
>sp|Q62829|PAK3_RAT Serine/threonine-protein kinase PAK 3 (p21-activated kinase 3)
(PAK-3) (Beta-PAK) (p65-PAK)
Length = 544
Score = 102 bits (255), Expect = 6e-22
Identities = 56/169 (33%), Positives = 89/169 (52%), Gaps = 1/169 (0%)
Frame = +2
Query: 8 EPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGF-MSAKMFPDASSET 184
E I ++ L+ALDFLHS ++HRD+K ILL + +KL F A++ P+ S +
Sbjct: 361 EGQIAAVCRECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRS 420
Query: 185 FRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYR 364
+ GT W APEV+ + YG DIWS G + +E++ G+ P +A+Y
Sbjct: 421 -------TMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYL 473
Query: 365 LGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFLNSA 511
+ T E P + S + F++ CL+ + R + K+L++H FL A
Sbjct: 474 IATNGTPELQNPERLSAVFRDFLNRCLEMDVDRRGSAKELLQHPFLKLA 522
>sp|O75914|PAK3_HUMAN Serine/threonine-protein kinase PAK 3 (p21-activated kinase 3)
(PAK-3) (Beta-PAK) (Oligophrenin-3)
sp|Q7YQL3|PAK3_PONPY Serine/threonine-protein kinase PAK 3 (p21-activated kinase 3)
(PAK-3)
sp|Q7YQL4|PAK3_PANTR Serine/threonine-protein kinase PAK 3 (p21-activated kinase 3)
(PAK-3)
Length = 559
Score = 102 bits (255), Expect = 6e-22
Identities = 56/169 (33%), Positives = 89/169 (52%), Gaps = 1/169 (0%)
Frame = +2
Query: 8 EPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGF-MSAKMFPDASSET 184
E I ++ L+ALDFLHS ++HRD+K ILL + +KL F A++ P+ S +
Sbjct: 376 EGQIAAVCRECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRS 435
Query: 185 FRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYR 364
+ GT W APEV+ + YG DIWS G + +E++ G+ P +A+Y
Sbjct: 436 -------TMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYL 488
Query: 365 LGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFLNSA 511
+ T E P + S + F++ CL+ + R + K+L++H FL A
Sbjct: 489 IATNGTPELQNPERLSAVFRDFLNRCLEMDVDRRGSAKELLQHPFLKLA 537
>sp|Q61036|PAK3_MOUSE Serine/threonine-protein kinase PAK 3 (p21-activated kinase 3)
(PAK-3) (Beta-PAK) (CDC42/RAC effector kinase PAK-B)
Length = 559
Score = 102 bits (255), Expect = 6e-22
Identities = 56/169 (33%), Positives = 89/169 (52%), Gaps = 1/169 (0%)
Frame = +2
Query: 8 EPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGF-MSAKMFPDASSET 184
E I ++ L+ALDFLHS ++HRD+K ILL + +KL F A++ P+ S +
Sbjct: 376 EGQIAAVCRECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRS 435
Query: 185 FRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYR 364
+ GT W APEV+ + YG DIWS G + +E++ G+ P +A+Y
Sbjct: 436 -------TMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYL 488
Query: 365 LGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFLNSA 511
+ T E P + S + F++ CL+ + R + K+L++H FL A
Sbjct: 489 IATNGTPELQNPERLSAVFRDFLNRCLEMDVDRRGSAKELLQHPFLKLA 537
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 73,465,315
Number of Sequences: 369166
Number of extensions: 1495869
Number of successful extensions: 6982
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5286
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5560
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4748907085
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)