Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_021_M13-2
(507 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P20287|G3P_SCHMA Glyceraldehyde-3-phosphate dehydrogenas... 244 6e-65
sp|P04406|G3P2_HUMAN Glyceraldehyde-3-phosphate dehydrogena... 243 1e-64
sp|Q28259|G3P_CANFA Glyceraldehyde-3-phosphate dehydrogenas... 243 2e-64
sp|P00355|G3P_PIG Glyceraldehyde-3-phosphate dehydrogenase ... 242 3e-64
sp|Q9N2D5|G3P_FELCA Glyceraldehyde-3-phosphate dehydrogenas... 241 7e-64
sp|P46406|G3P_RABIT Glyceraldehyde-3-phosphate dehydrogenas... 241 9e-64
sp|O01360|G3P_ONCVO Glyceraldehyde 3-phosphate-dehydrogenas... 241 9e-64
sp|P48812|G3P_BRUMA Glyceraldehyde-3-phosphate dehydrogenas... 241 9e-64
sp|P00356|G3P_CHICK Glyceraldehyde-3-phosphate dehydrogenas... 240 1e-63
sp|P00354|G3P1_HUMAN Glyceraldehyde-3-phosphate dehydrogena... 240 2e-63
>sp|P20287|G3P_SCHMA Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Major larval
surface antigen) (P-37)
Length = 338
Score = 244 bits (624), Expect = 6e-65
Identities = 119/167 (71%), Positives = 140/167 (83%)
Frame = +3
Query: 6 MTKPKVGINGFGRIGRLVLRAALEKGTVDVVALNDPFIELDYMVYMFKYDSTHGKFHGHV 185
M++ KVGINGFGRIGRLVLRAA K TVDVV++NDPFI+L+YMVYM K DSTHG F G V
Sbjct: 1 MSRAKVGINGFGRIGRLVLRAAFLKNTVDVVSVNDPFIDLEYMVYMIKRDSTHGTFPGEV 60
Query: 186 EAKDGKLVVDGHAMSVYNKRNPDEIPWDHDHCYYVVESTGVFTTIEKASAHTKNNRAKKV 365
++GKL V+G +SV+ +R+P IPWD D YVVESTGVFTTI+KA AH KNNRAKKV
Sbjct: 61 STENGKLKVNGKLISVHCERDPANIPWDKDGAEYVVESTGVFTTIDKAQAHIKNNRAKKV 120
Query: 366 VISAPSADAPMFVMGVNHEKYDPSLSIVSNASCTTNCLAPLAKVVND 506
+ISAPSADAPMFV+GVN Y+ S+S+VSNASCTTNCLAPLAKV++D
Sbjct: 121 IISAPSADAPMFVVGVNENSYEKSMSVVSNASCTTNCLAPLAKVIHD 167
>sp|P04406|G3P2_HUMAN Glyceraldehyde-3-phosphate dehydrogenase, liver (GAPDH)
Length = 335
Score = 243 bits (621), Expect = 1e-64
Identities = 116/167 (69%), Positives = 142/167 (85%)
Frame = +3
Query: 6 MTKPKVGINGFGRIGRLVLRAALEKGTVDVVALNDPFIELDYMVYMFKYDSTHGKFHGHV 185
M K KVG+NGFGRIGRLV RAA G VD+VA+NDPFI+L+YMVYMF+YDSTHGKFHG V
Sbjct: 1 MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTV 60
Query: 186 EAKDGKLVVDGHAMSVYNKRNPDEIPWDHDHCYYVVESTGVFTTIEKASAHTKNNRAKKV 365
+A++GKLV++G+ ++++ +R+P +I W YVVESTGVFTT+EKA AH + AK+V
Sbjct: 61 KAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGG-AKRV 119
Query: 366 VISAPSADAPMFVMGVNHEKYDPSLSIVSNASCTTNCLAPLAKVVND 506
+ISAPSADAPMFVMGVNHEKYD SL I+SNASCTTNCLAPLAKV++D
Sbjct: 120 IISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHD 166
>sp|Q28259|G3P_CANFA Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Length = 333
Score = 243 bits (620), Expect = 2e-64
Identities = 117/163 (71%), Positives = 139/163 (85%)
Frame = +3
Query: 18 KVGINGFGRIGRLVLRAALEKGTVDVVALNDPFIELDYMVYMFKYDSTHGKFHGHVEAKD 197
KVG+NGFGRIGRLV RAA G VD+VA+NDPFI+L+YMVYMF+YDSTHGKFHG V+A++
Sbjct: 3 KVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAEN 62
Query: 198 GKLVVDGHAMSVYNKRNPDEIPWDHDHCYYVVESTGVFTTIEKASAHTKNNRAKKVVISA 377
GKLV++G ++S++ +R+P I W YVVESTGVFTT+EKA AH K AK+V+ISA
Sbjct: 63 GKLVINGKSISIFQERDPANIKWGDAGAEYVVESTGVFTTMEKAGAHLKGG-AKRVIISA 121
Query: 378 PSADAPMFVMGVNHEKYDPSLSIVSNASCTTNCLAPLAKVVND 506
PSADAPMFVMGVNHEKYD SL IVSNASCTTNCLAPLAKV++D
Sbjct: 122 PSADAPMFVMGVNHEKYDNSLKIVSNASCTTNCLAPLAKVIHD 164
>sp|P00355|G3P_PIG Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Length = 333
Score = 242 bits (618), Expect = 3e-64
Identities = 117/163 (71%), Positives = 138/163 (84%)
Frame = +3
Query: 18 KVGINGFGRIGRLVLRAALEKGTVDVVALNDPFIELDYMVYMFKYDSTHGKFHGHVEAKD 197
KVG+NGFGRIGRLV RAA G VD+VA+NDPFI+L YMVYMF+YDSTHGKFHG V+A++
Sbjct: 3 KVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLHYMVYMFQYDSTHGKFHGTVKAEN 62
Query: 198 GKLVVDGHAMSVYNKRNPDEIPWDHDHCYYVVESTGVFTTIEKASAHTKNNRAKKVVISA 377
GKLV++G A++++ +R+P I W YVVESTGVFTT+EKA AH K AK+V+ISA
Sbjct: 63 GKLVINGKAITIFQERDPANIKWGDAGATYVVESTGVFTTMEKAGAHLKGG-AKRVIISA 121
Query: 378 PSADAPMFVMGVNHEKYDPSLSIVSNASCTTNCLAPLAKVVND 506
PSADAPMFVMGVNHEKYD SL IVSNASCTTNCLAPLAKV++D
Sbjct: 122 PSADAPMFVMGVNHEKYDNSLKIVSNASCTTNCLAPLAKVIHD 164
>sp|Q9N2D5|G3P_FELCA Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Length = 333
Score = 241 bits (615), Expect = 7e-64
Identities = 116/163 (71%), Positives = 138/163 (84%)
Frame = +3
Query: 18 KVGINGFGRIGRLVLRAALEKGTVDVVALNDPFIELDYMVYMFKYDSTHGKFHGHVEAKD 197
KVG+NGFGRIGRLV RAA G VD+VA+NDPFI+L+YMVYMF+YDSTHGKFHG V+A++
Sbjct: 3 KVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAEN 62
Query: 198 GKLVVDGHAMSVYNKRNPDEIPWDHDHCYYVVESTGVFTTIEKASAHTKNNRAKKVVISA 377
GKLV++G ++++ +R+P I W YVVESTGVFTT+EKA AH K AK+V+ISA
Sbjct: 63 GKLVINGKPITIFQERDPANIKWGDAGAEYVVESTGVFTTMEKAGAHLKGG-AKRVIISA 121
Query: 378 PSADAPMFVMGVNHEKYDPSLSIVSNASCTTNCLAPLAKVVND 506
PSADAPMFVMGVNHEKYD SL IVSNASCTTNCLAPLAKV++D
Sbjct: 122 PSADAPMFVMGVNHEKYDNSLKIVSNASCTTNCLAPLAKVIHD 164
>sp|P46406|G3P_RABIT Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Length = 333
Score = 241 bits (614), Expect = 9e-64
Identities = 117/163 (71%), Positives = 137/163 (84%)
Frame = +3
Query: 18 KVGINGFGRIGRLVLRAALEKGTVDVVALNDPFIELDYMVYMFKYDSTHGKFHGHVEAKD 197
KVG+NGFGRIGRLV RAA G VDVVA+NDPFI+L YMVYMF+YDSTHGKFHG V+A++
Sbjct: 3 KVGVNGFGRIGRLVTRAAFNSGKVDVVAINDPFIDLHYMVYMFQYDSTHGKFHGTVKAEN 62
Query: 198 GKLVVDGHAMSVYNKRNPDEIPWDHDHCYYVVESTGVFTTIEKASAHTKNNRAKKVVISA 377
GKLV++G A++++ +R+P I W YVVESTGVFTT+EKA AH K AK+V+ISA
Sbjct: 63 GKLVINGKAITIFQERDPANIKWGDAGAEYVVESTGVFTTMEKAGAHLKGG-AKRVIISA 121
Query: 378 PSADAPMFVMGVNHEKYDPSLSIVSNASCTTNCLAPLAKVVND 506
PS DAPMFVMGVNHEKYD SL IVSNASCTTNCLAPLAKV++D
Sbjct: 122 PSXDAPMFVMGVNHEKYDNSLKIVSNASCTTNCLAPLAKVIHD 164
>sp|O01360|G3P_ONCVO Glyceraldehyde 3-phosphate-dehydrogenase (GAPDH) (Larval antigen
OvB95)
Length = 339
Score = 241 bits (614), Expect = 9e-64
Identities = 121/172 (70%), Positives = 144/172 (83%), Gaps = 5/172 (2%)
Frame = +3
Query: 6 MTKPKVGINGFGRIGRLVLRAALEKGTVDVVALNDPFIELDYMVYMFKYDSTHGKFHGHV 185
M+KPK+GINGFGRIGRLVLRAA+EK TV+VVA+NDPFI +DYMVYMFKYDSTHG+F GHV
Sbjct: 1 MSKPKIGINGFGRIGRLVLRAAVEKDTVEVVAVNDPFINIDYMVYMFKYDSTHGRFKGHV 60
Query: 186 EAKDGKLVVDG----HAMSVYNKRNPDEIPWDHDHCYYVVESTGVFTTIEKASAHTKNNR 353
A+ GKL+V H ++V+N ++P EIPW + YVVESTGVFT EKASAH K
Sbjct: 61 SAEGGKLIVTNGKTTHQIAVHNSKDPAEIPWGVEGAEYVVESTGVFTHTEKASAHLKGG- 119
Query: 354 AKKVVISAPSADAPMFVMGVNHEKYDPSLS-IVSNASCTTNCLAPLAKVVND 506
AKKV+ISAPSADAPMFVMGVN++KYD + + I+SNASCTTNCLAPLAKV++D
Sbjct: 120 AKKVIISAPSADAPMFVMGVNNDKYDKANNHIISNASCTTNCLAPLAKVIHD 171
>sp|P48812|G3P_BRUMA Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Length = 339
Score = 241 bits (614), Expect = 9e-64
Identities = 123/172 (71%), Positives = 143/172 (83%), Gaps = 5/172 (2%)
Frame = +3
Query: 6 MTKPKVGINGFGRIGRLVLRAALEKGTVDVVALNDPFIELDYMVYMFKYDSTHGKFHGHV 185
M+KPKVGINGFGRIGRLVLRAA+EK TVDVVA+NDPFI +DYMVYMFKYDSTHG+F G V
Sbjct: 1 MSKPKVGINGFGRIGRLVLRAAVEKDTVDVVAVNDPFINIDYMVYMFKYDSTHGRFKGSV 60
Query: 186 EAKDGKLVVDG----HAMSVYNKRNPDEIPWDHDHCYYVVESTGVFTTIEKASAHTKNNR 353
A+ GKL+V H +SV+N ++P EIPW D YVVESTGVFTT +KASAH K
Sbjct: 61 SAEGGKLIVTNGKTTHHISVHNSKDPAEIPWGVDGAEYVVESTGVFTTTDKASAHLKGG- 119
Query: 354 AKKVVISAPSADAPMFVMGVNHEKYDPSLS-IVSNASCTTNCLAPLAKVVND 506
AKKV+ISAPSADAPMFVMGVN++ YD + + I+SNASCTTNCLAPLAKV++D
Sbjct: 120 AKKVIISAPSADAPMFVMGVNNDMYDKANNHIISNASCTTNCLAPLAKVIHD 171
>sp|P00356|G3P_CHICK Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Length = 333
Score = 240 bits (613), Expect = 1e-63
Identities = 117/163 (71%), Positives = 138/163 (84%)
Frame = +3
Query: 18 KVGINGFGRIGRLVLRAALEKGTVDVVALNDPFIELDYMVYMFKYDSTHGKFHGHVEAKD 197
KVG+NGFGRIGRLV RAA+ G V VVA+NDPFI+L+YMVYMFKYDSTHG F G V+A++
Sbjct: 3 KVGVNGFGRIGRLVTRAAVLSGKVQVVAINDPFIDLNYMVYMFKYDSTHGHFKGTVKAEN 62
Query: 198 GKLVVDGHAMSVYNKRNPDEIPWDHDHCYYVVESTGVFTTIEKASAHTKNNRAKKVVISA 377
GKLV++GHA++++ +R+P I W YVVESTGVFTT+EKA AH K AK+V+ISA
Sbjct: 63 GKLVINGHAITIFQERDPSNIKWADAGAEYVVESTGVFTTMEKAGAHLKGG-AKRVIISA 121
Query: 378 PSADAPMFVMGVNHEKYDPSLSIVSNASCTTNCLAPLAKVVND 506
PSADAPMFVMGVNHEKYD SL IVSNASCTTNCLAPLAKV++D
Sbjct: 122 PSADAPMFVMGVNHEKYDKSLKIVSNASCTTNCLAPLAKVIHD 164
>sp|P00354|G3P1_HUMAN Glyceraldehyde-3-phosphate dehydrogenase, muscle (GAPDH)
Length = 334
Score = 240 bits (612), Expect = 2e-63
Identities = 116/165 (70%), Positives = 138/165 (83%)
Frame = +3
Query: 12 KPKVGINGFGRIGRLVLRAALEKGTVDVVALNDPFIELDYMVYMFKYDSTHGKFHGHVEA 191
K KVG++GFGRIGRLV RAA G VD+VA+NDPFI+L YMVYMF+YDSTHGKFHG V+A
Sbjct: 2 KVKVGVDGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLHYMVYMFQYDSTHGKFHGTVKA 61
Query: 192 KDGKLVVDGHAMSVYNKRNPDEIPWDHDHCYYVVESTGVFTTIEKASAHTKNNRAKKVVI 371
+DGKLV+DG A++++ +R+P+ I W YVVESTGVFTT+EKA AH K AK++VI
Sbjct: 62 EDGKLVIDGKAITIFQERDPENIKWGDAGTAYVVESTGVFTTMEKAGAHLKGG-AKRIVI 120
Query: 372 SAPSADAPMFVMGVNHEKYDPSLSIVSNASCTTNCLAPLAKVVND 506
SAPSADAPMFVMGVNH KY SL I+SNASCTTNCLAPLAKV++D
Sbjct: 121 SAPSADAPMFVMGVNHFKYANSLKIISNASCTTNCLAPLAKVIHD 165
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 61,813,684
Number of Sequences: 369166
Number of extensions: 1249944
Number of successful extensions: 3640
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3174
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3253
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3206272875
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)