Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_021_M03
(436 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9D1E6|TBCB_MOUSE Tubulin-specific chaperone B (Tubulin ... 122 4e-28
sp|Q99426|TBCB_HUMAN Tubulin-specific chaperone B (Tubulin ... 120 2e-27
sp|Q5E951|TBCB_BOVIN Tubulin-specific chaperone B (Tubulin ... 120 2e-27
sp|Q20728|YXHK_CAEEL Hypothetical protein F53F4.3 in chromo... 107 1e-23
sp|Q9VJE5|CL190_DROME Restin homolog (Cytoplasmic linker pr... 72 7e-13
sp|Q01397|DYNA_NEUCR Dynactin, 150 kDa isoform (150 kDa dyn... 70 3e-12
sp|P30622|REST_HUMAN Restin (Cytoplasmic linker protein 170... 69 6e-12
sp|Q9UDT6|CYLN2_HUMAN Cytoplasmic linker protein 2 (Cytopla... 68 1e-11
sp|O42184|REST_CHICK Restin (Cytoplasmic linker protein 170... 67 1e-11
sp|Q8CI96|RSNL2_MOUSE Restin-like protein 2 67 1e-11
>sp|Q9D1E6|TBCB_MOUSE Tubulin-specific chaperone B (Tubulin folding cofactor B)
(Cytoskeleton associated protein 1)
(Cytoskeleton-associated protein CKAPI)
Length = 244
Score = 122 bits (305), Expect = 4e-28
Identities = 52/87 (59%), Positives = 66/87 (75%), Gaps = 2/87 (2%)
Frame = +3
Query: 30 SLKISDRCEVRVPNQSAKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECP 209
++ + RCEVR P+ S +RG + Y+G T FKPGYWVGV YDEP GKN+G+V+GK YFEC
Sbjct: 158 AISVGSRCEVRAPDHSLRRGTVMYVGLTDFKPGYWVGVRYDEPLGKNDGSVNGKRYFECQ 217
Query: 210 EKYGAFIKPQHITAGDFPE--LGLDDL 284
KYGAF+KP +T GDFPE GLD++
Sbjct: 218 AKYGAFVKPSAVTVGDFPEEDYGLDEM 244
>sp|Q99426|TBCB_HUMAN Tubulin-specific chaperone B (Tubulin folding cofactor B)
(Cytoskeleton associated protein 1)
(Cytoskeleton-associated protein CKAPI)
Length = 244
Score = 120 bits (300), Expect = 2e-27
Identities = 52/87 (59%), Positives = 65/87 (74%), Gaps = 2/87 (2%)
Frame = +3
Query: 30 SLKISDRCEVRVPNQSAKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECP 209
S+ + RCEVR QS +RG + Y+G T FKPGYW+GV YDEP GKN+G+V+GK YFEC
Sbjct: 158 SIPVGSRCEVRAAGQSPRRGTVMYVGLTDFKPGYWIGVRYDEPLGKNDGSVNGKRYFECQ 217
Query: 210 EKYGAFIKPQHITAGDFPE--LGLDDL 284
KYGAF+KP +T GDFPE GLD++
Sbjct: 218 AKYGAFVKPAVVTVGDFPEEDYGLDEI 244
>sp|Q5E951|TBCB_BOVIN Tubulin-specific chaperone B (Tubulin folding cofactor B)
(Cytoskeleton associated protein 1)
(Cytoskeleton-associated protein CKAPI)
Length = 244
Score = 120 bits (300), Expect = 2e-27
Identities = 50/87 (57%), Positives = 65/87 (74%), Gaps = 2/87 (2%)
Frame = +3
Query: 30 SLKISDRCEVRVPNQSAKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECP 209
++ + RCEVR P Q +RG + Y+G T FKPGYW+G+ YDEP GKN+G+V+GK YFEC
Sbjct: 158 TIPVGSRCEVRTPGQPPRRGTVMYVGLTDFKPGYWIGIRYDEPLGKNDGSVNGKRYFECQ 217
Query: 210 EKYGAFIKPQHITAGDFPE--LGLDDL 284
KYGAF+KP +T GDFPE GLD++
Sbjct: 218 AKYGAFVKPSVVTVGDFPEEDYGLDEM 244
>sp|Q20728|YXHK_CAEEL Hypothetical protein F53F4.3 in chromosome V
Length = 229
Score = 107 bits (267), Expect = 1e-23
Identities = 44/86 (51%), Positives = 62/86 (72%)
Frame = +3
Query: 27 ESLKISDRCEVRVPNQSAKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFEC 206
+++ + +RCEV V Q A+RG++ Y+G T FK G WVGV YDEP GKN+G+V G YF+C
Sbjct: 144 KNIMVGNRCEVTVGAQMARRGEVAYVGATKFKEGVWVGVKYDEPVGKNDGSVAGVRYFDC 203
Query: 207 PEKYGAFIKPQHITAGDFPELGLDDL 284
KYG F++P + GDFPEL +D++
Sbjct: 204 DPKYGGFVRPVDVKVGDFPELSIDEI 229
>sp|Q9VJE5|CL190_DROME Restin homolog (Cytoplasmic linker protein 190) (Microtubule
binding protein 190) (d-CLIP-190)
Length = 1690
Score = 71.6 bits (174), Expect = 7e-13
Identities = 33/70 (47%), Positives = 43/70 (61%)
Frame = +3
Query: 21 GRESLKISDRCEVRVPNQSAKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYF 200
G+ + + DR V ++ G ++Y+G T F PG W GV DEPSGKN+GTVD YF
Sbjct: 233 GKNGMAVGDRVIVS-SGFGSRPGILRYLGETQFAPGNWCGVELDEPSGKNDGTVDDIRYF 291
Query: 201 ECPEKYGAFI 230
EC KYG F+
Sbjct: 292 ECKPKYGVFV 301
Score = 65.1 bits (157), Expect = 6e-11
Identities = 29/54 (53%), Positives = 35/54 (64%)
Frame = +3
Query: 87 GQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIKPQHIT 248
GQI +IG THF G W GV DEP+GKN+G V GK YF+C K G F + +T
Sbjct: 137 GQIAFIGDTHFAAGEWAGVVLDEPNGKNDGCVSGKRYFQCEPKRGIFSRLTRLT 190
>sp|Q01397|DYNA_NEUCR Dynactin, 150 kDa isoform (150 kDa dynein-associated polypeptide)
(DP-150) (DAP-150) (p150-glued)
Length = 1300
Score = 69.7 bits (169), Expect = 3e-12
Identities = 27/61 (44%), Positives = 41/61 (67%)
Frame = +3
Query: 54 EVRVPNQSAKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIK 233
++ + + S + ++Y+G T F PG WVG+ DEPSGKN+G+V G+ YF C YG F++
Sbjct: 11 KIELADGSGRTAFVRYVGETAFAPGTWVGIELDEPSGKNDGSVQGERYFNCEMGYGMFVR 70
Query: 234 P 236
P
Sbjct: 71 P 71
>sp|P30622|REST_HUMAN Restin (Cytoplasmic linker protein 170 alpha-2) (CLIP-170)
(Reed-Sternberg intermediate filament associated
protein) (Cytoplasmic linker protein 1)
Length = 1427
Score = 68.6 bits (166), Expect = 6e-12
Identities = 35/81 (43%), Positives = 42/81 (51%)
Frame = +3
Query: 21 GRESLKISDRCEVRVPNQSAKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYF 200
G LKI DR V K G ++++G T F G W GV DEP GKN+G V G YF
Sbjct: 208 GERELKIGDRVLVG----GTKAGVVRFLGETDFAKGEWCGVELDEPLGKNDGAVAGTRYF 263
Query: 201 ECPEKYGAFIKPQHITAGDFP 263
+C KYG F +T FP
Sbjct: 264 QCQPKYGLFAPVHKVTKIGFP 284
Score = 60.8 bits (146), Expect = 1e-09
Identities = 26/56 (46%), Positives = 35/56 (62%)
Frame = +3
Query: 81 KRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIKPQHIT 248
K G I+++G T F PG W G+ DEP GKN+G+V G YF+C G F +P +T
Sbjct: 70 KPGFIQFLGETQFAPGQWAGIVLDEPIGKNDGSVAGVRYFQCEPLKGIFTRPSKLT 125
>sp|Q9UDT6|CYLN2_HUMAN Cytoplasmic linker protein 2 (Cytoplasmic linker protein 115)
(CLIP-115) (Williams-Beuren syndrome chromosome region 4
protein)
Length = 1046
Score = 67.8 bits (164), Expect = 1e-11
Identities = 33/81 (40%), Positives = 43/81 (53%)
Frame = +3
Query: 21 GRESLKISDRCEVRVPNQSAKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYF 200
G + L++ DR V K G ++Y+G T F G W GV DEP GKN+G V G YF
Sbjct: 215 GEKDLRLGDRVLVG----GTKTGVVRYVGETDFAKGEWCGVELDEPLGKNDGAVAGTRYF 270
Query: 201 ECPEKYGAFIKPQHITAGDFP 263
+CP K+G F + FP
Sbjct: 271 QCPPKFGLFAPIHKVIRIGFP 291
Score = 67.0 bits (162), Expect = 2e-11
Identities = 30/63 (47%), Positives = 37/63 (58%)
Frame = +3
Query: 60 RVPNQSAKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIKPQ 239
RV K G ++Y+G T F PG W GV D+P GKN+G V G YFECP G F +P
Sbjct: 84 RVWVNGVKPGVVQYLGETQFAPGQWAGVVLDDPVGKNDGAVGGVRYFECPALQGIFTRPS 143
Query: 240 HIT 248
+T
Sbjct: 144 KLT 146
>sp|O42184|REST_CHICK Restin (Cytoplasmic linker protein 170) (CLIP-170)
Length = 1433
Score = 67.4 bits (163), Expect = 1e-11
Identities = 34/81 (41%), Positives = 42/81 (51%)
Frame = +3
Query: 21 GRESLKISDRCEVRVPNQSAKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYF 200
G LKI DR V K G ++++G T F G W GV DEP GKN+G V G YF
Sbjct: 211 GERELKIGDRVLVG----GTKAGVVRFLGETDFAKGEWCGVELDEPLGKNDGAVAGTRYF 266
Query: 201 ECPEKYGAFIKPQHITAGDFP 263
+C +YG F +T FP
Sbjct: 267 QCQPRYGLFAPVHKVTKIGFP 287
Score = 59.3 bits (142), Expect = 3e-09
Identities = 25/56 (44%), Positives = 35/56 (62%)
Frame = +3
Query: 81 KRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIKPQHIT 248
K G I+++G T F PG W G+ DEP GKN+G+V G YF+C G F +P ++
Sbjct: 71 KPGFIQFLGETQFAPGQWAGIVLDEPIGKNDGSVAGVRYFQCEPLRGIFTRPSKLS 126
>sp|Q8CI96|RSNL2_MOUSE Restin-like protein 2
Length = 704
Score = 67.4 bits (163), Expect = 1e-11
Identities = 26/53 (49%), Positives = 35/53 (66%)
Frame = +3
Query: 87 GQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIKPQHI 245
G IK+ GTT+F PGYW G+ ++P GKN+G+V G YF C +YG F P +
Sbjct: 498 GTIKFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRV 550
Score = 62.4 bits (150), Expect = 4e-10
Identities = 29/65 (44%), Positives = 37/65 (56%)
Frame = +3
Query: 33 LKISDRCEVRVPNQSAKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPE 212
LK+ DR + K G +++ GTT F G W G+ DEP GKNNG+V YF+C
Sbjct: 283 LKLGDRVVIA----GQKVGTLRFCGTTEFASGQWAGIELDEPEGKNNGSVGRVQYFKCAP 338
Query: 213 KYGAF 227
KYG F
Sbjct: 339 KYGIF 343
Score = 54.3 bits (129), Expect = 1e-07
Identities = 21/58 (36%), Positives = 31/58 (53%)
Frame = +3
Query: 75 SAKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIKPQHIT 248
S + ++Y+G T F G W+G+ GKN+G V K YF C YG ++P +T
Sbjct: 633 SNEMATVRYVGPTDFASGIWLGLELRSAKGKNDGAVGDKRYFTCKPNYGVLVRPSRVT 690
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 53,274,447
Number of Sequences: 369166
Number of extensions: 1107289
Number of successful extensions: 2425
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2383
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2424
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2194785120
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)