Planarian EST Database


Dr_sW_021_J24

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_021_J24
         (881 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P13230|GRP33_ARTSA  Glycine-rich protein GRP33                  88   4e-17
sp|Q60749|SAM68_MOUSE  KH domain containing, RNA binding, si...    79   2e-14
sp|Q07666|SAM68_HUMAN  KH domain containing, RNA binding, si...    79   2e-14
sp|Q91V33|SAM68_RAT  KH domain containing, RNA binding, sign...    79   2e-14
sp|Q8UUW7|SAM68_CHICK  KH domain containing, RNA binding, si...    78   3e-14
sp|O01367|HOW_DROME  Held out wings protein (KH-domain prote...    59   2e-08
sp|Q15637|SF01_HUMAN  Splicing factor 1 (Zinc finger protein...    56   2e-07
sp|Q64213|SF01_MOUSE  Splicing factor 1 (Zinc finger protein...    54   4e-07
sp|Q17339|GLD1_CAEEL  Female germline-specific tumor suppres...    53   1e-06
sp|Q96I24|FUBP3_HUMAN  Far upstream element-binding protein ...    34   0.62 
>sp|P13230|GRP33_ARTSA Glycine-rich protein GRP33
          Length = 308

 Score = 87.8 bits (216), Expect = 4e-17
 Identities = 47/125 (37%), Positives = 79/125 (63%), Gaps = 10/125 (8%)
 Frame = +2

Query: 188 YIDLILNKKVKVRAKVDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRG----I 355
           ++DL  + KVK+ ++  +P DQ+P  NF+GKLLGPG ST+K +Q++T TK++I G     
Sbjct: 63  FMDLYNDTKVKLVSRCCLPVDQFPKYNFLGKLLGPGGSTMKQLQDETMTKISILGRGSMR 122

Query: 356 DNQKKNVISNADQC------EKLHLQIDSLGTPAEAYYKLSHAVAEVQKCLTFNLPLSYP 517
           D  K+  + N+         E+LH++I S+ +PAEA+ ++++A+ E++K +T       P
Sbjct: 123 DRNKEEELRNSGDVKYAHLNEQLHIEIISIASPAEAHARMAYALTEIKKYIT-------P 175

Query: 518 TVDPN 532
             DPN
Sbjct: 176 EEDPN 180
>sp|Q60749|SAM68_MOUSE KH domain containing, RNA binding, signal transduction associated
           protein 1 (p21 Ras GTPase-activating protein-associated
           p62) (GAP-associated tyrosine phosphoprotein p62)
           (Src-associated in mitosis 68 kDa protein) (Sam68) (p68)
          Length = 443

 Score = 78.6 bits (192), Expect = 2e-14
 Identities = 45/128 (35%), Positives = 73/128 (57%), Gaps = 16/128 (12%)
 Frame = +2

Query: 155 EIDRLRGGIA------DYIDLILNKKVKVRAKVDIPADQYPTTNFVGKLLGPGASTLKSI 316
           EI++++ G +      +Y+DL  +K +K++ +V IP  QYP  NFVGK+LGP  +T+K +
Sbjct: 128 EIEKIQKGESKKDDEENYLDLFSHKNMKLKERVLIPVKQYPKFNFVGKILGPQGNTIKRL 187

Query: 317 QEQTNTKMTIRG----IDNQKKNVISNADQCEKLHLQID------SLGTPAEAYYKLSHA 466
           QE+T  K+++ G     D  K+  +      +  HL +D        G P EAY  ++HA
Sbjct: 188 QEETGAKISVLGKGSMRDKAKEEELRKGGDPKYAHLNMDLHVFIEVFGPPCEAYALMAHA 247

Query: 467 VAEVQKCL 490
           + EV+K L
Sbjct: 248 MEEVKKFL 255
>sp|Q07666|SAM68_HUMAN KH domain containing, RNA binding, signal transduction associated
           protein 1 (p21 Ras GTPase-activating protein-associated
           p62) (GAP-associated tyrosine phosphoprotein p62)
           (Src-associated in mitosis 68 kDa protein) (Sam68) (p68)
          Length = 443

 Score = 78.6 bits (192), Expect = 2e-14
 Identities = 45/128 (35%), Positives = 73/128 (57%), Gaps = 16/128 (12%)
 Frame = +2

Query: 155 EIDRLRGGIA------DYIDLILNKKVKVRAKVDIPADQYPTTNFVGKLLGPGASTLKSI 316
           EI++++ G +      +Y+DL  +K +K++ +V IP  QYP  NFVGK+LGP  +T+K +
Sbjct: 128 EIEKIQKGDSKKDDEENYLDLFSHKNMKLKERVLIPVKQYPKFNFVGKILGPQGNTIKRL 187

Query: 317 QEQTNTKMTIRG----IDNQKKNVISNADQCEKLHLQID------SLGTPAEAYYKLSHA 466
           QE+T  K+++ G     D  K+  +      +  HL +D        G P EAY  ++HA
Sbjct: 188 QEETGAKISVLGKGSMRDKAKEEELRKGGDPKYAHLNMDLHVFIEVFGPPCEAYALMAHA 247

Query: 467 VAEVQKCL 490
           + EV+K L
Sbjct: 248 MEEVKKFL 255
>sp|Q91V33|SAM68_RAT KH domain containing, RNA binding, signal transduction associated
           protein 1 (p21 Ras GTPase-activating protein-associated
           p62) (GAP-associated tyrosine phosphoprotein p62)
           (Src-associated in mitosis 68 kDa protein) (Sam68) (p68)
          Length = 443

 Score = 78.6 bits (192), Expect = 2e-14
 Identities = 45/128 (35%), Positives = 73/128 (57%), Gaps = 16/128 (12%)
 Frame = +2

Query: 155 EIDRLRGGIA------DYIDLILNKKVKVRAKVDIPADQYPTTNFVGKLLGPGASTLKSI 316
           EI++++ G +      +Y+DL  +K +K++ +V IP  QYP  NFVGK+LGP  +T+K +
Sbjct: 128 EIEKIQKGESKKDDEENYLDLFSHKNMKLKERVLIPVKQYPKFNFVGKILGPQGNTIKRL 187

Query: 317 QEQTNTKMTIRG----IDNQKKNVISNADQCEKLHLQID------SLGTPAEAYYKLSHA 466
           QE+T  K+++ G     D  K+  +      +  HL +D        G P EAY  ++HA
Sbjct: 188 QEETGAKISVLGKGSMRDKAKEEELRKGGDPKYAHLNMDLHVFIEVFGPPCEAYALMAHA 247

Query: 467 VAEVQKCL 490
           + EV+K L
Sbjct: 248 MEEVKKFL 255
>sp|Q8UUW7|SAM68_CHICK KH domain containing, RNA binding, signal transduction associated
           protein 1 (Src-associated in mitosis 68 kDa protein)
           (Sam68)
          Length = 433

 Score = 78.2 bits (191), Expect = 3e-14
 Identities = 42/112 (37%), Positives = 65/112 (58%), Gaps = 10/112 (8%)
 Frame = +2

Query: 185 DYIDLILNKKVKVRAKVDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRG---- 352
           +Y+DL  +K +K++ +V IP  QYP  NFVGK+LGP  +T+K +QE+T  K+++ G    
Sbjct: 127 NYLDLFSHKNMKLKERVLIPVKQYPKFNFVGKILGPQGNTIKRLQEETGAKISVLGKGSM 186

Query: 353 IDNQKKNVISNADQCEKLHLQID------SLGTPAEAYYKLSHAVAEVQKCL 490
            D  K+  +      +  HL +D        G P EAY  ++HA+ EV+K L
Sbjct: 187 RDKAKEEELRKGGDPKYAHLNMDLHVFIEVFGPPCEAYALMAHAMEEVKKFL 238
>sp|O01367|HOW_DROME Held out wings protein (KH-domain protein KH93F) (Putative
           RNA-binding protein) (Muscle-specific protein) (Wings
           held out protein) (Struthio protein) (Quaking-related
           93F)
          Length = 405

 Score = 58.9 bits (141), Expect = 2e-08
 Identities = 34/100 (34%), Positives = 54/100 (54%), Gaps = 8/100 (8%)
 Frame = +2

Query: 215 VKVRAKVDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIDNQKKNVISNADQ 394
           V +  KV +P  ++P  NFVG++LGP   T K ++++T  K+ +RG  + +     +A++
Sbjct: 135 VTMNEKVYVPVREHPDFNFVGRILGPRGMTAKQLEQETGCKIMVRGKGSMRDKKKEDANR 194

Query: 395 --------CEKLHLQIDSLGTPAEAYYKLSHAVAEVQKCL 490
                    + LH+ I    T   A  KL+ AVAEVQK L
Sbjct: 195 GKPNWEHLSDDLHVLITVEDTENRATVKLAQAVAEVQKLL 234
>sp|Q15637|SF01_HUMAN Splicing factor 1 (Zinc finger protein 162) (Transcription factor
           ZFM1) (Zinc finger gene in MEN1 locus) (Mammalian branch
           point binding protein mBBP) (BBP)
          Length = 639

 Score = 55.8 bits (133), Expect = 2e-07
 Identities = 26/58 (44%), Positives = 38/58 (65%)
 Frame = +2

Query: 218 KVRAKVDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIDNQKKNVISNAD 391
           +V  KV IP D+YP  NFVG L+GP  +TLK+I+++ N K+ IRG  + K+  +   D
Sbjct: 135 RVSDKVMIPQDEYPEINFVGLLIGPRGNTLKNIEKECNAKIMIRGKGSVKEGKVGRKD 192
>sp|Q64213|SF01_MOUSE Splicing factor 1 (Zinc finger protein 162) (Transcription factor
           ZFM1) (mZFM) (Zinc finger gene in MEN1 locus) (Mammalian
           branch point binding protein mBBP) (BBP) (CW17)
          Length = 653

 Score = 54.3 bits (129), Expect = 4e-07
 Identities = 25/58 (43%), Positives = 38/58 (65%)
 Frame = +2

Query: 218 KVRAKVDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIDNQKKNVISNAD 391
           +V  KV IP D+YP  NFVG L+GP  +TLK+I+++ N K+ IRG  + ++  +   D
Sbjct: 135 RVSDKVMIPQDEYPEINFVGLLIGPRGNTLKNIEKECNAKIMIRGKGSVEEGKVGRKD 192
>sp|Q17339|GLD1_CAEEL Female germline-specific tumor suppressor gld-1 (Defective in germ
           line development protein 1)
          Length = 463

 Score = 52.8 bits (125), Expect = 1e-06
 Identities = 27/100 (27%), Positives = 51/100 (51%), Gaps = 8/100 (8%)
 Frame = +2

Query: 215 VKVRAKVDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIDNQKKNVISNADQ 394
           + +  K+ +P ++YP  NFVG++LGP   T K +++ T  K+ +RG  + +     +A +
Sbjct: 203 ISITEKIYVPKNEYPDYNFVGRILGPRGMTAKQLEQDTGCKIMVRGKGSMRDKSKESAHR 262

Query: 395 C--------EKLHLQIDSLGTPAEAYYKLSHAVAEVQKCL 490
                    + LH+ +    T    + KL  A+ +V+K L
Sbjct: 263 GKANWEHLEDDLHVLVQCEDTENRVHIKLQAALEQVKKLL 302
>sp|Q96I24|FUBP3_HUMAN Far upstream element-binding protein 3 (FUSE-binding protein 3)
          Length = 572

 Score = 33.9 bits (76), Expect = 0.62
 Identities = 20/63 (31%), Positives = 34/63 (53%)
 Frame = +2

Query: 158 IDRLRGGIADYIDLILNKKVKVRAKVDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTK 337
           +DR R G   + D+  N  ++   ++ IPA +      VG ++G G  T+K +QE+T  K
Sbjct: 145 VDRCRNGPGFHNDIDSNSTIQ---EILIPASK------VGLVIGRGGETIKQLQERTGVK 195

Query: 338 MTI 346
           M +
Sbjct: 196 MVM 198
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 96,304,697
Number of Sequences: 369166
Number of extensions: 1830985
Number of successful extensions: 3714
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3620
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3708
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8790245790
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)