Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_021_J07
(844 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P13230|GRP33_ARTSA Glycine-rich protein GRP33 88 3e-17
sp|Q60749|SAM68_MOUSE KH domain containing, RNA binding, si... 79 2e-14
sp|Q07666|SAM68_HUMAN KH domain containing, RNA binding, si... 79 2e-14
sp|Q91V33|SAM68_RAT KH domain containing, RNA binding, sign... 79 2e-14
sp|Q8UUW7|SAM68_CHICK KH domain containing, RNA binding, si... 78 3e-14
sp|O01367|HOW_DROME Held out wings protein (KH-domain prote... 59 2e-08
sp|Q15637|SF01_HUMAN Splicing factor 1 (Zinc finger protein... 56 1e-07
sp|Q64213|SF01_MOUSE Splicing factor 1 (Zinc finger protein... 54 4e-07
sp|Q17339|GLD1_CAEEL Female germline-specific tumor suppres... 53 1e-06
sp|Q96I24|FUBP3_HUMAN Far upstream element-binding protein ... 34 0.58
>sp|P13230|GRP33_ARTSA Glycine-rich protein GRP33
Length = 308
Score = 87.8 bits (216), Expect = 3e-17
Identities = 47/125 (37%), Positives = 79/125 (63%), Gaps = 10/125 (8%)
Frame = +2
Query: 152 YIDLILNKKVKVRAKVDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRG----I 319
++DL + KVK+ ++ +P DQ+P NF+GKLLGPG ST+K +Q++T TK++I G
Sbjct: 63 FMDLYNDTKVKLVSRCCLPVDQFPKYNFLGKLLGPGGSTMKQLQDETMTKISILGRGSMR 122
Query: 320 DNQKKNVISNADQC------EKLHLQIDSLGTPAEAYYKLSHAVAEVQKCLTFNLPLSYP 481
D K+ + N+ E+LH++I S+ +PAEA+ ++++A+ E++K +T P
Sbjct: 123 DRNKEEELRNSGDVKYAHLNEQLHIEIISIASPAEAHARMAYALTEIKKYIT-------P 175
Query: 482 TVDPN 496
DPN
Sbjct: 176 EEDPN 180
>sp|Q60749|SAM68_MOUSE KH domain containing, RNA binding, signal transduction associated
protein 1 (p21 Ras GTPase-activating protein-associated
p62) (GAP-associated tyrosine phosphoprotein p62)
(Src-associated in mitosis 68 kDa protein) (Sam68) (p68)
Length = 443
Score = 78.6 bits (192), Expect = 2e-14
Identities = 45/128 (35%), Positives = 73/128 (57%), Gaps = 16/128 (12%)
Frame = +2
Query: 119 EIDRLRGGIA------DYIDLILNKKVKVRAKVDIPADQYPTTNFVGKLLGPGASTLKSI 280
EI++++ G + +Y+DL +K +K++ +V IP QYP NFVGK+LGP +T+K +
Sbjct: 128 EIEKIQKGESKKDDEENYLDLFSHKNMKLKERVLIPVKQYPKFNFVGKILGPQGNTIKRL 187
Query: 281 QEQTNTKMTIRG----IDNQKKNVISNADQCEKLHLQID------SLGTPAEAYYKLSHA 430
QE+T K+++ G D K+ + + HL +D G P EAY ++HA
Sbjct: 188 QEETGAKISVLGKGSMRDKAKEEELRKGGDPKYAHLNMDLHVFIEVFGPPCEAYALMAHA 247
Query: 431 VAEVQKCL 454
+ EV+K L
Sbjct: 248 MEEVKKFL 255
>sp|Q07666|SAM68_HUMAN KH domain containing, RNA binding, signal transduction associated
protein 1 (p21 Ras GTPase-activating protein-associated
p62) (GAP-associated tyrosine phosphoprotein p62)
(Src-associated in mitosis 68 kDa protein) (Sam68) (p68)
Length = 443
Score = 78.6 bits (192), Expect = 2e-14
Identities = 45/128 (35%), Positives = 73/128 (57%), Gaps = 16/128 (12%)
Frame = +2
Query: 119 EIDRLRGGIA------DYIDLILNKKVKVRAKVDIPADQYPTTNFVGKLLGPGASTLKSI 280
EI++++ G + +Y+DL +K +K++ +V IP QYP NFVGK+LGP +T+K +
Sbjct: 128 EIEKIQKGDSKKDDEENYLDLFSHKNMKLKERVLIPVKQYPKFNFVGKILGPQGNTIKRL 187
Query: 281 QEQTNTKMTIRG----IDNQKKNVISNADQCEKLHLQID------SLGTPAEAYYKLSHA 430
QE+T K+++ G D K+ + + HL +D G P EAY ++HA
Sbjct: 188 QEETGAKISVLGKGSMRDKAKEEELRKGGDPKYAHLNMDLHVFIEVFGPPCEAYALMAHA 247
Query: 431 VAEVQKCL 454
+ EV+K L
Sbjct: 248 MEEVKKFL 255
>sp|Q91V33|SAM68_RAT KH domain containing, RNA binding, signal transduction associated
protein 1 (p21 Ras GTPase-activating protein-associated
p62) (GAP-associated tyrosine phosphoprotein p62)
(Src-associated in mitosis 68 kDa protein) (Sam68) (p68)
Length = 443
Score = 78.6 bits (192), Expect = 2e-14
Identities = 45/128 (35%), Positives = 73/128 (57%), Gaps = 16/128 (12%)
Frame = +2
Query: 119 EIDRLRGGIA------DYIDLILNKKVKVRAKVDIPADQYPTTNFVGKLLGPGASTLKSI 280
EI++++ G + +Y+DL +K +K++ +V IP QYP NFVGK+LGP +T+K +
Sbjct: 128 EIEKIQKGESKKDDEENYLDLFSHKNMKLKERVLIPVKQYPKFNFVGKILGPQGNTIKRL 187
Query: 281 QEQTNTKMTIRG----IDNQKKNVISNADQCEKLHLQID------SLGTPAEAYYKLSHA 430
QE+T K+++ G D K+ + + HL +D G P EAY ++HA
Sbjct: 188 QEETGAKISVLGKGSMRDKAKEEELRKGGDPKYAHLNMDLHVFIEVFGPPCEAYALMAHA 247
Query: 431 VAEVQKCL 454
+ EV+K L
Sbjct: 248 MEEVKKFL 255
>sp|Q8UUW7|SAM68_CHICK KH domain containing, RNA binding, signal transduction associated
protein 1 (Src-associated in mitosis 68 kDa protein)
(Sam68)
Length = 433
Score = 78.2 bits (191), Expect = 3e-14
Identities = 42/112 (37%), Positives = 65/112 (58%), Gaps = 10/112 (8%)
Frame = +2
Query: 149 DYIDLILNKKVKVRAKVDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRG---- 316
+Y+DL +K +K++ +V IP QYP NFVGK+LGP +T+K +QE+T K+++ G
Sbjct: 127 NYLDLFSHKNMKLKERVLIPVKQYPKFNFVGKILGPQGNTIKRLQEETGAKISVLGKGSM 186
Query: 317 IDNQKKNVISNADQCEKLHLQID------SLGTPAEAYYKLSHAVAEVQKCL 454
D K+ + + HL +D G P EAY ++HA+ EV+K L
Sbjct: 187 RDKAKEEELRKGGDPKYAHLNMDLHVFIEVFGPPCEAYALMAHAMEEVKKFL 238
>sp|O01367|HOW_DROME Held out wings protein (KH-domain protein KH93F) (Putative
RNA-binding protein) (Muscle-specific protein) (Wings
held out protein) (Struthio protein) (Quaking-related
93F)
Length = 405
Score = 58.9 bits (141), Expect = 2e-08
Identities = 34/100 (34%), Positives = 54/100 (54%), Gaps = 8/100 (8%)
Frame = +2
Query: 179 VKVRAKVDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIDNQKKNVISNADQ 358
V + KV +P ++P NFVG++LGP T K ++++T K+ +RG + + +A++
Sbjct: 135 VTMNEKVYVPVREHPDFNFVGRILGPRGMTAKQLEQETGCKIMVRGKGSMRDKKKEDANR 194
Query: 359 --------CEKLHLQIDSLGTPAEAYYKLSHAVAEVQKCL 454
+ LH+ I T A KL+ AVAEVQK L
Sbjct: 195 GKPNWEHLSDDLHVLITVEDTENRATVKLAQAVAEVQKLL 234
>sp|Q15637|SF01_HUMAN Splicing factor 1 (Zinc finger protein 162) (Transcription factor
ZFM1) (Zinc finger gene in MEN1 locus) (Mammalian branch
point binding protein mBBP) (BBP)
Length = 639
Score = 55.8 bits (133), Expect = 1e-07
Identities = 26/58 (44%), Positives = 38/58 (65%)
Frame = +2
Query: 182 KVRAKVDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIDNQKKNVISNAD 355
+V KV IP D+YP NFVG L+GP +TLK+I+++ N K+ IRG + K+ + D
Sbjct: 135 RVSDKVMIPQDEYPEINFVGLLIGPRGNTLKNIEKECNAKIMIRGKGSVKEGKVGRKD 192
>sp|Q64213|SF01_MOUSE Splicing factor 1 (Zinc finger protein 162) (Transcription factor
ZFM1) (mZFM) (Zinc finger gene in MEN1 locus) (Mammalian
branch point binding protein mBBP) (BBP) (CW17)
Length = 653
Score = 54.3 bits (129), Expect = 4e-07
Identities = 25/58 (43%), Positives = 38/58 (65%)
Frame = +2
Query: 182 KVRAKVDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIDNQKKNVISNAD 355
+V KV IP D+YP NFVG L+GP +TLK+I+++ N K+ IRG + ++ + D
Sbjct: 135 RVSDKVMIPQDEYPEINFVGLLIGPRGNTLKNIEKECNAKIMIRGKGSVEEGKVGRKD 192
>sp|Q17339|GLD1_CAEEL Female germline-specific tumor suppressor gld-1 (Defective in germ
line development protein 1)
Length = 463
Score = 52.8 bits (125), Expect = 1e-06
Identities = 27/100 (27%), Positives = 51/100 (51%), Gaps = 8/100 (8%)
Frame = +2
Query: 179 VKVRAKVDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIDNQKKNVISNADQ 358
+ + K+ +P ++YP NFVG++LGP T K +++ T K+ +RG + + +A +
Sbjct: 203 ISITEKIYVPKNEYPDYNFVGRILGPRGMTAKQLEQDTGCKIMVRGKGSMRDKSKESAHR 262
Query: 359 C--------EKLHLQIDSLGTPAEAYYKLSHAVAEVQKCL 454
+ LH+ + T + KL A+ +V+K L
Sbjct: 263 GKANWEHLEDDLHVLVQCEDTENRVHIKLQAALEQVKKLL 302
>sp|Q96I24|FUBP3_HUMAN Far upstream element-binding protein 3 (FUSE-binding protein 3)
Length = 572
Score = 33.9 bits (76), Expect = 0.58
Identities = 20/63 (31%), Positives = 34/63 (53%)
Frame = +2
Query: 122 IDRLRGGIADYIDLILNKKVKVRAKVDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTK 301
+DR R G + D+ N ++ ++ IPA + VG ++G G T+K +QE+T K
Sbjct: 145 VDRCRNGPGFHNDIDSNSTIQ---EILIPASK------VGLVIGRGGETIKQLQERTGVK 195
Query: 302 MTI 310
M +
Sbjct: 196 MVM 198
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 95,300,550
Number of Sequences: 369166
Number of extensions: 1903018
Number of successful extensions: 5049
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4842
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5041
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8245425915
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)