Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_021_H24
(620 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P23588|IF4B_HUMAN Eukaryotic translation initiation fact... 64 3e-10
sp|Q8BGD9|IF4B_MOUSE Eukaryotic translation initiation fact... 62 9e-10
sp|O14369|SCE3_SCHPO Probable RNA-binding protein sce3 47 3e-05
sp|Q9P7Y9|SIN1_SCHPO Stress-activated map kinase interactin... 36 0.069
sp|Q09511|RSP4_CAEEL Probable splicing factor, arginine/ser... 34 0.26
sp|Q9NTZ6|RBM12_HUMAN RNA-binding protein 12 (RNA-binding m... 33 0.58
sp|P37838|NOP4_YEAST Nucleolar protein NOP4 (Nucleolar prot... 33 0.58
sp|Q8SQ27|RBM12_MACMU RNA-binding protein 12 (RNA-binding m... 33 0.58
sp|Q8R4X3|RBM12_MOUSE RNA-binding protein 12 (RNA-binding m... 33 0.76
sp|Q04836|ROC3_ARATH 31 kDa ribonucleoprotein, chloroplast ... 32 1.00
>sp|P23588|IF4B_HUMAN Eukaryotic translation initiation factor 4B (eIF-4B)
Length = 611
Score = 63.9 bits (154), Expect = 3e-10
Identities = 50/168 (29%), Positives = 79/168 (47%), Gaps = 9/168 (5%)
Frame = +2
Query: 92 RSVLPTKTSATRQIS-DLENIPQNGPFLLNFSNIPYDADENAIRQFFSNYDVINV----- 253
RS+LPT A R+ + D +P++ P+ N+PYD E +I++FF ++ V
Sbjct: 70 RSILPTAPRAAREPNIDRSRLPKSPPYTAFLGNLPYDVTEESIKEFFRGLNISAVRLpre 129
Query: 254 -TKNEIKRGTGIIEFKDLNNLSGALSLAEKIFLNRPIRLSIS--LKDDLSRIKSGGXXXX 424
+ E +G G EF+DL++L ALSL E+ NR IR+ ++ +D +S G
Sbjct: 130 PSNPERLKGFGYAEFEDLDSLLSALSLNEESLGNRRIRVDVADQAQDKDRDDRSFGRDR- 188
Query: 425 XXXXXXXXXPPTISEDSDNWRTKKPQPVSQPSTDNYTPRNDRSNWRDK 568
+ DSD T + S D+Y PR ++ DK
Sbjct: 189 -------------NRDSDKTDTDWRARPATDSFDDYPPRRGDDSFGDK 223
>sp|Q8BGD9|IF4B_MOUSE Eukaryotic translation initiation factor 4B (eIF-4B)
Length = 611
Score = 62.4 bits (150), Expect = 9e-10
Identities = 49/168 (29%), Positives = 79/168 (47%), Gaps = 9/168 (5%)
Frame = +2
Query: 92 RSVLPTKTSATRQIS-DLENIPQNGPFLLNFSNIPYDADENAIRQFFSNYDVINV----- 253
RS+LPT A R+ + D +P++ P+ N+PYD E++I+ FF ++ V
Sbjct: 70 RSILPTAPRAAREPNIDRSRLPKSPPYTAFLGNLPYDVTEDSIKDFFRGLNISAVRLpre 129
Query: 254 -TKNEIKRGTGIIEFKDLNNLSGALSLAEKIFLNRPIRLSIS--LKDDLSRIKSGGXXXX 424
+ + +G G EF+DL++L ALSL E+ NR IR+ ++ +D +S G
Sbjct: 130 PSNPDRLKGFGYAEFEDLDSLLSALSLNEESLGNRRIRVDVADQAQDKDRDDRSFGRDR- 188
Query: 425 XXXXXXXXXPPTISEDSDNWRTKKPQPVSQPSTDNYTPRNDRSNWRDK 568
+ DSD T + S D+Y PR ++ DK
Sbjct: 189 -------------NRDSDKTDTDWRARPTTDSFDDYPPRRGDDSFGDK 223
>sp|O14369|SCE3_SCHPO Probable RNA-binding protein sce3
Length = 388
Score = 47.4 bits (111), Expect = 3e-05
Identities = 24/80 (30%), Positives = 44/80 (55%), Gaps = 4/80 (5%)
Frame = +2
Query: 149 IPQNGPFLLNFSNIPYDADENAIRQFF----SNYDVINVTKNEIKRGTGIIEFKDLNNLS 316
IP PF + N+ +D EN + FF ++ ++ E RG G +EF+ + LS
Sbjct: 88 IPSEPPFTAHVGNLSFDLTENDLGDFFGEGVTSIRLVIDPLTERSRGFGYVEFETADTLS 147
Query: 317 GALSLAEKIFLNRPIRLSIS 376
AL+L+ + + RP+R++++
Sbjct: 148 AALALSGEDLMGRPVRITVA 167
>sp|Q9P7Y9|SIN1_SCHPO Stress-activated map kinase interacting protein 1 (SAPK interacting
protein 1)
Length = 665
Score = 36.2 bits (82), Expect = 0.069
Identities = 28/87 (32%), Positives = 41/87 (47%), Gaps = 17/87 (19%)
Frame = +2
Query: 11 DVPLTFSTKSNWSQDESDS--------------NTRDVSEIRSVLPTK--TSATRQISD- 139
++PLTF S+ + E+D+ V++ RSV K TSA R + +
Sbjct: 205 EIPLTFPMNSSLTDTEADAVVAVDALFPGKQRGTHNTVNKARSVSNAKAPTSALRALLEH 264
Query: 140 LENIPQNGPFLLNFSNIPYDADENAIR 220
EN QNGP NF+ A+ NA+R
Sbjct: 265 KENSSQNGPLAENFATFSGHAESNALR 291
>sp|Q09511|RSP4_CAEEL Probable splicing factor, arginine/serine-rich 4 (RNA-binding
protein srp-2) (CeSC35)
Length = 196
Score = 34.3 bits (77), Expect = 0.26
Identities = 37/165 (22%), Positives = 63/165 (38%), Gaps = 11/165 (6%)
Frame = +2
Query: 158 NGPFLLNFSNIPYDADENAIRQFFSNY-DV--INVTKNEIKR---GTGIIEFKDLNNLSG 319
NG L N+ Y N +R+ F Y D+ +++ +++ R G G + F + +
Sbjct: 16 NGLTSLKIDNLSYQTTPNDLRRTFERYGDIGDVHIPRDKYSRQSKGFGFVRFYERRDAEH 75
Query: 320 ALSLAE-KIFLNRPIRLSISLKDDLSRIKSGGXXXXXXXXXXXXXPPTISEDSDNWRTKK 496
AL + K+ R +R++++ D S + G R +
Sbjct: 76 ALDRTDGKLVDGRELRVTLAKYDRPSDERGG-------------------RGGGGGRRRS 116
Query: 497 PQPVSQPSTDNYT----PRNDRSNWRDKPSLIRNDDKALSDNSEN 619
P + + Y+ PR RS R PS R D DNS +
Sbjct: 117 RSPRRRSRSPRYSRSRSPRRSRSRTRSPPSRDRRDSPDRRDNSRS 161
>sp|Q9NTZ6|RBM12_HUMAN RNA-binding protein 12 (RNA-binding motif protein 12) (SH3/WW
domain anchor protein in the nucleus) (SWAN)
Length = 932
Score = 33.1 bits (74), Expect = 0.58
Identities = 25/107 (23%), Positives = 45/107 (42%), Gaps = 7/107 (6%)
Frame = +2
Query: 101 LPTKTSATRQISDLENIPQNGPFLLNFSNIPYDADENAIRQFFSNYDVINVT------KN 262
LP S + Q P F + +P++A+ + FF D++ + N
Sbjct: 408 LPRSKSPSGQKRSRSRSPHEAGFCVYLKGLPFEAENKHVIDFFKKLDIVEDSIYIAYGPN 467
Query: 263 EIKRGTGIIEFKDLNNLSGALSLAEKIFLNRPIRL-SISLKDDLSRI 400
G G +EF++ + AL ++ NR I++ I+ K L +I
Sbjct: 468 GKATGEGFVEFRNEADYKAALCRHKQYMGNRFIQVHPITKKGMLEKI 514
>sp|P37838|NOP4_YEAST Nucleolar protein NOP4 (Nucleolar protein NOP77)
Length = 685
Score = 33.1 bits (74), Expect = 0.58
Identities = 21/89 (23%), Positives = 36/89 (40%), Gaps = 6/89 (6%)
Frame = +2
Query: 53 DESDSNTRDVSEIRSVLPTKTSATRQISDLENIPQNGPFLLNFSNIPYDADENAIRQFFS 232
DE D N + ++ K S + + E+ F + N+PYDA E ++ FS
Sbjct: 258 DEEDENEEEDRQVDQASKNKESKRKAQNKRED------FSVFVRNVPYDATEESLAPHFS 311
Query: 233 NYDVINVT------KNEIKRGTGIIEFKD 301
+ + + +GT + FKD
Sbjct: 312 KFGSVKYALPVIDKSTGLAKGTAFVAFKD 340
Score = 31.6 bits (70), Expect = 1.7
Identities = 23/77 (29%), Positives = 37/77 (48%), Gaps = 6/77 (7%)
Frame = +2
Query: 185 NIPYDADENAIRQFFSNY-----DVINVTKNEIKRGTGIIEFKDLNNLSGALSLAEKIFL 349
+IP D + + FFSN+ V+ N+ RG G + F ++ AL+ A K
Sbjct: 32 SIPQDVTDEQLADFFSNFAPIKHAVVVKDTNKRSRGFGFVSFAVEDDTKEALAKARKTKF 91
Query: 350 NRPI-RLSISLKDDLSR 397
N I R+ I+ + D S+
Sbjct: 92 NGHILRVDIAKRRDRSK 108
>sp|Q8SQ27|RBM12_MACMU RNA-binding protein 12 (RNA-binding motif protein 12) (SH3/WW
domain anchor protein in the nucleus) (SWAN)
Length = 932
Score = 33.1 bits (74), Expect = 0.58
Identities = 25/107 (23%), Positives = 45/107 (42%), Gaps = 7/107 (6%)
Frame = +2
Query: 101 LPTKTSATRQISDLENIPQNGPFLLNFSNIPYDADENAIRQFFSNYDVINVT------KN 262
LP S + Q P F + +P++A+ + FF D++ + N
Sbjct: 408 LPRSKSPSGQKRSRSRSPHEAGFCVYLKGLPFEAENKHVIDFFKKLDIVEDSIYIAYGPN 467
Query: 263 EIKRGTGIIEFKDLNNLSGALSLAEKIFLNRPIRL-SISLKDDLSRI 400
G G +EF++ + AL ++ NR I++ I+ K L +I
Sbjct: 468 GKATGEGFVEFRNEADYKAALCRHKQYMGNRFIQVHPITKKGMLEKI 514
>sp|Q8R4X3|RBM12_MOUSE RNA-binding protein 12 (RNA-binding motif protein 12) (SH3/WW
domain anchor protein in the nucleus) (SWAN)
Length = 1002
Score = 32.7 bits (73), Expect = 0.76
Identities = 25/107 (23%), Positives = 45/107 (42%), Gaps = 7/107 (6%)
Frame = +2
Query: 101 LPTKTSATRQISDLENIPQNGPFLLNFSNIPYDADENAIRQFFSNYDVINVT------KN 262
LP S + Q P F + +P++A+ + FF D++ + N
Sbjct: 408 LPRSKSPSGQKRSRSRSPHEAGFCVYLKGLPFEAENKHVIDFFKKLDIVEDSIYIAYGPN 467
Query: 263 EIKRGTGIIEFKDLNNLSGALSLAEKIFLNRPIRL-SISLKDDLSRI 400
G G +EF++ + AL ++ NR I++ I+ K L +I
Sbjct: 468 GKATGEGFVEFRNDADYKAALCRHKQYMGNRFIQVHPITKKGMLEKI 514
>sp|Q04836|ROC3_ARATH 31 kDa ribonucleoprotein, chloroplast precursor (RNA-binding
protein RNP-T) (RNA-binding protein 1/2/3) (AtRBP33)
(RNA-binding protein cp31)
Length = 329
Score = 32.3 bits (72), Expect = 1.00
Identities = 30/126 (23%), Positives = 55/126 (43%), Gaps = 9/126 (7%)
Frame = +2
Query: 26 FSTKSNWSQDESDSNTRDVSEIRSVLPTKTSATRQISDLENIPQ--NGPFLLNFSNIPYD 199
F T S+ + E+ + ++ L T A + S E P+ F + N+P+D
Sbjct: 195 FVTMSSVDEAETAVEKFNRYDLNGRLLTVNKAAPRGSRPERAPRVYEPAFRVYVGNLPWD 254
Query: 200 ADENAIRQFFSNYD------VINVTKNEIKRGTGIIEFKDLNNLSGALSLAEKIFL-NRP 358
D + Q FS + V+ + RG G + D++ L+ A+S + L R
Sbjct: 255 VDNGRLEQLFSEHGKVVEARVVYDRETGRSRGFGFVTMSDVDELNEAISALDGQNLEGRA 314
Query: 359 IRLSIS 376
IR++++
Sbjct: 315 IRVNVA 320
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 64,313,339
Number of Sequences: 369166
Number of extensions: 1198732
Number of successful extensions: 3713
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3601
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3706
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 4877307000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)