Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_021_G02
(232 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P19075|TSN8_HUMAN Tetraspanin-8 (Tspan-8) (Transmembrane... 45 5e-05
sp|P62080|TSN5_MOUSE Tetraspanin-5 (Tspan-5) (Transmembrane... 42 3e-04
sp|Q68VK5|TSN5_RAT Tetraspanin-5 (Tspan-5) (Transmembrane 4... 42 3e-04
sp|P19331|IM23_SCHMA 23 kDa integral membrane protein (SM23) 40 0.001
sp|P27591|IM23_SCHJA 23 kDa integral membrane protein (SJ23) 40 0.002
sp|Q26499|IM23_SCHHA 23 kDa integral membrane protein (Sh23) 40 0.002
sp|P28648|CD63_RAT CD63 antigen (AD1 antigen) 39 0.005
sp|O60636|TSN2_HUMAN Tetraspanin-2 (Tspan-2) 39 0.005
sp|P41731|CD63_MOUSE CD63 antigen 39 0.005
sp|Q922J6|TSN2_MOUSE Tetraspanin-2 (Tspan-2) 38 0.008
>sp|P19075|TSN8_HUMAN Tetraspanin-8 (Tspan-8) (Transmembrane 4 superfamily member 3)
(Tumor-associated antigen CO-029)
Length = 237
Score = 45.1 bits (105), Expect = 5e-05
Identities = 24/81 (29%), Positives = 48/81 (59%), Gaps = 4/81 (4%)
Frame = +2
Query: 2 FGFWVIGLSLTVISSFF-LRNDAWKF--SDETVTHKYVIIGILVT-GTIMFLIAFAGWCG 169
F FW+ G+ + ++ + + ND+ S++ + YV + IL+ G I+ ++ F G CG
Sbjct: 17 FLFWLCGILILALAIWVRVSNDSQAIFGSEDVGSSSYVAVDILIAVGAIIMILGFLGCCG 76
Query: 170 ALKESTFLLRIYVISITVIIV 232
A+KES +L ++ I + +I++
Sbjct: 77 AIKESRCMLLLFFIGLLLILL 97
>sp|P62080|TSN5_MOUSE Tetraspanin-5 (Tspan-5) (Transmembrane 4 superfamily member 9)
(Tetraspan NET-4)
sp|P62079|TSN5_HUMAN Tetraspanin-5 (Tspan-5) (Transmembrane 4 superfamily member 9)
(Tetraspan NET-4)
Length = 268
Score = 42.4 bits (98), Expect = 3e-04
Identities = 25/83 (30%), Positives = 42/83 (50%), Gaps = 10/83 (12%)
Frame = +2
Query: 8 FWVIGLSLTVISSFFLRNDAWKFSDETVTHKYVIIG----------ILVTGTIMFLIAFA 157
FW +G++ FL W ++++ V I LV G +MF++ FA
Sbjct: 26 FWFLGIT-------FLGIGLWAWNEKGVLSNISSITDLGGFDPVWLFLVVGGVMFILGFA 78
Query: 158 GWCGALKESTFLLRIYVISITVI 226
G GAL+E+TFLL+ + + + +I
Sbjct: 79 GCIGALRENTFLLKFFSVFLGII 101
>sp|Q68VK5|TSN5_RAT Tetraspanin-5 (Tspan-5) (Transmembrane 4 superfamily member 9)
Length = 268
Score = 42.4 bits (98), Expect = 3e-04
Identities = 25/83 (30%), Positives = 42/83 (50%), Gaps = 10/83 (12%)
Frame = +2
Query: 8 FWVIGLSLTVISSFFLRNDAWKFSDETVTHKYVIIG----------ILVTGTIMFLIAFA 157
FW +G++ FL W ++++ V I LV G +MF++ FA
Sbjct: 26 FWFLGIT-------FLGIGLWAWNEKGVLSNISSITDLGGFDPVWLFLVVGGVMFILGFA 78
Query: 158 GWCGALKESTFLLRIYVISITVI 226
G GAL+E+TFLL+ + + + +I
Sbjct: 79 GCIGALRENTFLLKFFSVFLGII 101
>sp|P19331|IM23_SCHMA 23 kDa integral membrane protein (SM23)
Length = 218
Score = 40.4 bits (93), Expect = 0.001
Identities = 15/41 (36%), Positives = 28/41 (68%)
Frame = +2
Query: 110 IGILVTGTIMFLIAFAGWCGALKESTFLLRIYVISITVIIV 232
I I+V G I+ +++F G CGA+KE+ +L +Y + V+++
Sbjct: 56 IAIIVVGVIILIVSFLGCCGAIKENVCMLYMYAFFLVVLLI 96
>sp|P27591|IM23_SCHJA 23 kDa integral membrane protein (SJ23)
Length = 218
Score = 39.7 bits (91), Expect = 0.002
Identities = 14/41 (34%), Positives = 28/41 (68%)
Frame = +2
Query: 110 IGILVTGTIMFLIAFAGWCGALKESTFLLRIYVISITVIIV 232
I I+V G ++ +++F G CGA+KE+ +L +Y + V+++
Sbjct: 56 IAIIVVGVVILIVSFLGCCGAIKENVCMLYMYAFFLIVLLI 96
>sp|Q26499|IM23_SCHHA 23 kDa integral membrane protein (Sh23)
Length = 218
Score = 39.7 bits (91), Expect = 0.002
Identities = 14/41 (34%), Positives = 28/41 (68%)
Frame = +2
Query: 110 IGILVTGTIMFLIAFAGWCGALKESTFLLRIYVISITVIIV 232
I I+V G I+ +++F G CGA+KE+ +L +Y + ++++
Sbjct: 56 IAIIVVGVIILIVSFLGCCGAIKENVCMLYMYAFFLIILLI 96
>sp|P28648|CD63_RAT CD63 antigen (AD1 antigen)
Length = 238
Score = 38.5 bits (88), Expect = 0.005
Identities = 17/50 (34%), Positives = 30/50 (60%)
Frame = +2
Query: 83 ETVTHKYVIIGILVTGTIMFLIAFAGWCGALKESTFLLRIYVISITVIIV 232
ET + + I+ G +FL+AF G CGA KE+ L+ + I +++I++
Sbjct: 46 ETTAGSLLPVVIIAVGAFLFLVAFVGCCGACKENYCLMITFAIFLSLIML 95
>sp|O60636|TSN2_HUMAN Tetraspanin-2 (Tspan-2)
Length = 221
Score = 38.5 bits (88), Expect = 0.005
Identities = 23/77 (29%), Positives = 38/77 (49%), Gaps = 4/77 (5%)
Frame = +2
Query: 8 FWVIGLSLTVISSFFLRNDAWK-FSDETVTHKYVIIGILV---TGTIMFLIAFAGWCGAL 175
FW+ G ++ +F A K S E + +Y +G+ V G +M + F G CGA+
Sbjct: 22 FWLAGSAVIAFGLWFRFGGAIKELSSEDKSPEYFYVGLYVLVGAGALMMAVGFFGCCGAM 81
Query: 176 KESTFLLRIYVISITVI 226
+ES +L + + VI
Sbjct: 82 RESQCVLGSFFTCLLVI 98
>sp|P41731|CD63_MOUSE CD63 antigen
Length = 238
Score = 38.5 bits (88), Expect = 0.005
Identities = 17/50 (34%), Positives = 30/50 (60%)
Frame = +2
Query: 83 ETVTHKYVIIGILVTGTIMFLIAFAGWCGALKESTFLLRIYVISITVIIV 232
ET + + I+ G +FL+AF G CGA KE+ L+ + I +++I++
Sbjct: 46 ETTAGSLLPVVIIAVGAFLFLVAFVGCCGACKENYCLMITFAIFLSLIML 95
>sp|Q922J6|TSN2_MOUSE Tetraspanin-2 (Tspan-2)
Length = 221
Score = 37.7 bits (86), Expect = 0.008
Identities = 22/77 (28%), Positives = 37/77 (48%), Gaps = 4/77 (5%)
Frame = +2
Query: 8 FWVIGLSLTVISSFFLRNDAWK-FSDETVTHKYVIIGILV---TGTIMFLIAFAGWCGAL 175
FW+ G ++ +F K S E + +Y +G+ V G +M + F G CGA+
Sbjct: 22 FWLAGSAVIAFGLWFRFGGTMKDLSSEDKSPEYFYVGLYVLVGAGALMMTVGFFGCCGAM 81
Query: 176 KESTFLLRIYVISITVI 226
+ES +L + + VI
Sbjct: 82 RESQCVLGSFFTCLLVI 98
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,491,669
Number of Sequences: 369166
Number of extensions: 507492
Number of successful extensions: 1599
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1578
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1599
length of database: 68,354,980
effective HSP length: 47
effective length of database: 59,672,435
effective search space used: 1730500615
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)