Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_021_F23
(583 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q13247|SFRS6_HUMAN Splicing factor, arginine/serine-rich... 139 4e-33
sp|Q08170|SFRS4_HUMAN Splicing factor, arginine/serine-rich... 139 7e-33
sp|Q8VE97|SFRS4_MOUSE Splicing factor, arginine/serine-rich 4 139 7e-33
sp|P26686|SRR55_DROME Serine-arginine protein 55 (SRP55) (E... 130 2e-30
sp|Q09167|SFRS5_RAT Splicing factor, arginine/serine-rich 5... 127 2e-29
sp|Q13243|SFRS5_HUMAN Splicing factor, arginine/serine-rich... 126 4e-29
sp|O35326|SFRS5_MOUSE Splicing factor, arginine/serine-rich... 105 6e-23
sp|Q23121|RSP1_CAEEL Probable splicing factor, arginine/ser... 102 5e-22
sp|P78814|SRP2_SCHPO Pre-mRNA splicing factor srp2 101 1e-21
sp|Q23120|RSP2_CAEEL Probable splicing factor, arginine/ser... 99 8e-21
>sp|Q13247|SFRS6_HUMAN Splicing factor, arginine/serine-rich 6 (Pre-mRNA splicing factor
SRP55)
Length = 344
Score = 139 bits (351), Expect = 4e-33
Identities = 71/147 (48%), Positives = 91/147 (61%), Gaps = 16/147 (10%)
Frame = +2
Query: 2 GFVEFDSERDADDAIHDLHHKELAGGRVVLEHXXXXXXXXXXXXXXXXXNA--------- 154
GFVEF+ RDADDA+++L+ KEL G RV++EH
Sbjct: 38 GFVEFEDSRDADDAVYELNGKELCGERVIVEHARGPRRDRDGYSYGSRSGGGGYSSRRTS 97
Query: 155 -------PIRTDYRLLVENLPSRVSWQDLKDLMRKTGEVMFADAHKRRQNEGIVEFKTRA 313
P+RT+YRL+VENL SR SWQDLKD MR+ GEV +ADAHK R NEG++EF++ +
Sbjct: 98 GRDKYGPPVRTEYRLIVENLSSRCSWQDLKDFMRQAGEVTYADAHKERTNEGVIEFRSYS 157
Query: 314 DMKAALKKFDGYEISGRKLRVYEDNVR 394
DMK AL K DG EI+GR +R+ ED R
Sbjct: 158 DMKRALDKLDGTEINGRNIRLIEDKPR 184
>sp|Q08170|SFRS4_HUMAN Splicing factor, arginine/serine-rich 4 (Pre-mRNA splicing factor
SRP75) (SRP001LB)
Length = 494
Score = 139 bits (349), Expect = 7e-33
Identities = 71/143 (49%), Positives = 92/143 (64%), Gaps = 10/143 (6%)
Frame = +2
Query: 2 GFVEFDSERDADDAIHDLHHKELAGGRVVLEHXXXXXXXXXXXXXXXXXN---------- 151
GFVEFD RDADDA+++L+ K+L G RV++EH
Sbjct: 38 GFVEFDDLRDADDAVYELNGKDLCGERVIVEHARGPRRDGSYGSGRSGYGYRRSGRDKYG 97
Query: 152 APIRTDYRLLVENLPSRVSWQDLKDLMRKTGEVMFADAHKRRQNEGIVEFKTRADMKAAL 331
P RT+YRL+VENL SR SWQDLKD MR+ GEV +ADAHK R+NEG++EF + +DMK AL
Sbjct: 98 PPTRTEYRLIVENLSSRCSWQDLKDYMRQAGEVTYADAHKGRKNEGVIEFVSYSDMKRAL 157
Query: 332 KKFDGYEISGRKLRVYEDNVRGR 400
+K DG E++GRK+R+ ED R
Sbjct: 158 EKLDGTEVNGRKIRLVEDKPGSR 180
>sp|Q8VE97|SFRS4_MOUSE Splicing factor, arginine/serine-rich 4
Length = 489
Score = 139 bits (349), Expect = 7e-33
Identities = 71/143 (49%), Positives = 92/143 (64%), Gaps = 10/143 (6%)
Frame = +2
Query: 2 GFVEFDSERDADDAIHDLHHKELAGGRVVLEHXXXXXXXXXXXXXXXXXN---------- 151
GFVEFD RDADDA+++L+ K+L G RV++EH
Sbjct: 38 GFVEFDDLRDADDAVYELNGKDLCGERVIVEHARGPRRDGSYGSGRSGYGYRRSGRDKYG 97
Query: 152 APIRTDYRLLVENLPSRVSWQDLKDLMRKTGEVMFADAHKRRQNEGIVEFKTRADMKAAL 331
P RT+YRL+VENL SR SWQDLKD MR+ GEV +ADAHK R+NEG++EF + +DMK AL
Sbjct: 98 PPTRTEYRLIVENLSSRCSWQDLKDYMRQAGEVTYADAHKGRKNEGVIEFVSYSDMKRAL 157
Query: 332 KKFDGYEISGRKLRVYEDNVRGR 400
+K DG E++GRK+R+ ED R
Sbjct: 158 EKLDGTEVNGRKIRLVEDKPGSR 180
>sp|P26686|SRR55_DROME Serine-arginine protein 55 (SRP55) (Enhancer of deformed) (52-kDa
bracketing protein) (B52 protein)
Length = 376
Score = 130 bits (328), Expect = 2e-30
Identities = 70/158 (44%), Positives = 94/158 (59%), Gaps = 24/158 (15%)
Frame = +2
Query: 2 GFVEFDSERDADDAIHDLHHKELAGGRVVLEHXXXXXXXXXXXXXXXXXNA--------- 154
GFVEF+ RDADDA+++L+ KEL G RVV+E
Sbjct: 40 GFVEFEDYRDADDAVYELNGKELLGERVVVEPARGTARGSNRDRYDDRYGGRRGGGGGRY 99
Query: 155 ---------------PIRTDYRLLVENLPSRVSWQDLKDLMRKTGEVMFADAHKRRQNEG 289
P+RT+YRL+VENL SRVSWQDLKD MR+ GEV +ADAHK+R+NEG
Sbjct: 100 NEKNKNSRSSSRYGPPLRTEYRLIVENLSSRVSWQDLKDYMRQAGEVTYADAHKQRRNEG 159
Query: 290 IVEFKTRADMKAALKKFDGYEISGRKLRVYEDNVRGRT 403
+VEF + +DMK A++K D E++GR++ + ED GR+
Sbjct: 160 VVEFASLSDMKTAIEKLDDTELNGRRIHLVEDRRGGRS 197
>sp|Q09167|SFRS5_RAT Splicing factor, arginine/serine-rich 5 (Pre-mRNA splicing factor
SRP40) (Insulin-induced growth response protein CL-4)
(Delayed-early protein HRS)
Length = 269
Score = 127 bits (320), Expect = 2e-29
Identities = 69/146 (47%), Positives = 91/146 (62%), Gaps = 12/146 (8%)
Frame = +2
Query: 2 GFVEFDSERDADDAIHDLHHKELAGGRVVLEHXXXXXXXXXXXXXXXXX----------- 148
GFVEF+ RDADDA+++L KEL RV +EH
Sbjct: 40 GFVEFEDPRDADDAVYELDGKELCSERVTIEHARARSRGGRGRGRYSDRFSSRRPRNDRR 99
Query: 149 NAP-IRTDYRLLVENLPSRVSWQDLKDLMRKTGEVMFADAHKRRQNEGIVEFKTRADMKA 325
NAP +RT+ RL+VENL SRVSWQDLKD MR+ GEV FADAH+ + NEG+VEF + D+K
Sbjct: 100 NAPPVRTENRLIVENLSSRVSWQDLKDFMRQAGEVTFADAHRPKLNEGVVEFASYGDLKN 159
Query: 326 ALKKFDGYEISGRKLRVYEDNVRGRT 403
A++K G EI+GRK+++ E + R R+
Sbjct: 160 AIEKLSGKEINGRKIKLIEGSKRHRS 185
>sp|Q13243|SFRS5_HUMAN Splicing factor, arginine/serine-rich 5 (Pre-mRNA splicing factor
SRP40) (Delayed-early protein HRS)
Length = 272
Score = 126 bits (316), Expect = 4e-29
Identities = 68/143 (47%), Positives = 89/143 (62%), Gaps = 12/143 (8%)
Frame = +2
Query: 2 GFVEFDSERDADDAIHDLHHKELAGGRVVLEHXXXXXXXXXXXXXXXXX----------- 148
GFVEF+ RDADDA+++L KEL RV +EH
Sbjct: 40 GFVEFEDPRDADDAVYELDGKELCSERVTIEHARARSRGGRGRGRYSDRFSSRRPRNDRR 99
Query: 149 NAP-IRTDYRLLVENLPSRVSWQDLKDLMRKTGEVMFADAHKRRQNEGIVEFKTRADMKA 325
NAP +RT+ RL+VENL SRVSWQDLKD MR+ GEV FADAH+ + NEG+VEF + D+K
Sbjct: 100 NAPPVRTENRLIVENLSSRVSWQDLKDFMRQAGEVTFADAHRPKLNEGVVEFASYGDLKN 159
Query: 326 ALKKFDGYEISGRKLRVYEDNVR 394
A++K G EI+GRK+++ E + R
Sbjct: 160 AIEKLSGKEINGRKIKLIEGSKR 182
>sp|O35326|SFRS5_MOUSE Splicing factor, arginine/serine-rich 5 (Pre-mRNA splicing factor
SRP40) (Delayed-early protein HRS)
Length = 270
Score = 105 bits (263), Expect = 6e-23
Identities = 61/147 (41%), Positives = 83/147 (56%), Gaps = 13/147 (8%)
Frame = +2
Query: 2 GFVEFDSERDADDAIHDLHHKELAGGRVVLEHXXXXXXXXXXXXXXXXXNA--------- 154
GFVEF+ RDADDA+++L KEL RV +EH A
Sbjct: 40 GFVEFEDPRDADDAVYELDGKELCSERVTIEHARARSRGGRGRGRYSDRLAVADLGNDRR 99
Query: 155 ---PIRTDYRLLVENLPSRVSWQDLKDLMRKTGEVMFADAHKRRQNEGIVEFKTRADMKA 325
+ RL+VENL SRVSWQDLKD MR+ GEV FADAH+ + NEG+VEF + D+K
Sbjct: 100 KCSTCKNRNRLIVENLSSRVSWQDLKDFMRQAGEVTFADAHRPKLNEGVVEFASYGDLKN 159
Query: 326 ALKKFD-GYEISGRKLRVYEDNVRGRT 403
A++K +++G K ++ E + R R+
Sbjct: 160 AIEKLSWKRKLTGEKSKLIEGSKRHRS 186
>sp|Q23121|RSP1_CAEEL Probable splicing factor, arginine/serine-rich 1 (RNA-binding
protein srp-5) (CeSRp75)
Length = 312
Score = 102 bits (255), Expect = 5e-22
Identities = 60/169 (35%), Positives = 90/169 (53%), Gaps = 35/169 (20%)
Frame = +2
Query: 2 GFVEFDSERDADDAIHDLHHKELAGGRVVLEHXXXXXXXXXXXXXXXXXNA--------- 154
GFVEFD +RDA+DA+HDL+ KEL G RV+L++
Sbjct: 39 GFVEFDDKRDAEDAVHDLNGKELGGERVILDYSKPRGGGGDRGGFGGGGRGGARVSSYSG 98
Query: 155 -------------------------PIRTDYRLLVENLPSRVSWQDLKDLMRKTG-EVMF 256
P T +R++VENL SR+SWQDLKD +R+ G E +
Sbjct: 99 GGGGGRDRFDRYDRGPPRRESRYGRPYSTRHRVVVENLSSRISWQDLKDQVRRQGVEPTY 158
Query: 257 ADAHKRRQNEGIVEFKTRADMKAALKKFDGYEISGRKLRVYEDNVRGRT 403
A+AHK R NE ++ F T +D+K ++K DG +++GRK+++ +D+ GR+
Sbjct: 159 AEAHK-RPNEALLCFATPSDLKRCIEKCDGMDLNGRKIKMIDDSQAGRS 206
>sp|P78814|SRP2_SCHPO Pre-mRNA splicing factor srp2
Length = 365
Score = 101 bits (252), Expect = 1e-21
Identities = 55/130 (42%), Positives = 70/130 (53%), Gaps = 2/130 (1%)
Frame = +2
Query: 2 GFVEFDSERDADDAIHDLHHKELAGGRVVLEHXXXXXXXXXXXXXXXXXN--APIRTDYR 175
GFVE + RDA D ++D KE G R+V+E P RT +R
Sbjct: 40 GFVEVEDARDARDIVNDFQGKEFMGSRIVVEPARGERRRRENFRESAASKYPRPRRTGFR 99
Query: 176 LLVENLPSRVSWQDLKDLMRKTGEVMFADAHKRRQNEGIVEFKTRADMKAALKKFDGYEI 355
L+VENL VSWQDLKD+MRK GE F DAH+ G+VEF T DM+ AL +G I
Sbjct: 100 LIVENLSEDVSWQDLKDVMRKAGEPTFTDAHRENPGAGVVEFSTEEDMRNALTSLNGEVI 159
Query: 356 SGRKLRVYED 385
G+ + + ED
Sbjct: 160 KGQAVTLRED 169
>sp|Q23120|RSP2_CAEEL Probable splicing factor, arginine/serine-rich 2 (RNA-binding
protein srp-4) (CeSRp40)
Length = 281
Score = 99.0 bits (245), Expect = 8e-21
Identities = 57/153 (37%), Positives = 83/153 (54%), Gaps = 19/153 (12%)
Frame = +2
Query: 2 GFVEFDSERDADDAIHDLHHKELAGGRVVLEHXXXXXXXXXXXXXXXXX----------- 148
GFV+F +RDADDA+HDL+ KEL G RV+LE
Sbjct: 38 GFVDFQDQRDADDAVHDLNGKELCGERVILEFPRRKVGYNEERSGSGFRGREPTFRKGGE 97
Query: 149 -------NAPIRTDYRLLVENLPSRVSWQDLKDLMRKTG-EVMFADAHKRRQNEGIVEFK 304
+ P T +RL+++NL +R SWQD+KD +RK G E +++AHKR N+ IV F
Sbjct: 98 RQFSNRYSRPCSTRFRLVIDNLSTRYSWQDIKDHIRKLGIEPTYSEAHKRNVNQAIVCFT 157
Query: 305 TRADMKAALKKFDGYEISGRKLRVYEDNVRGRT 403
+ D++ A+ K G +++GRKL+ D R R+
Sbjct: 158 SHDDLRDAMNKLQGEDLNGRKLKC-TDETRDRS 189
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 55,942,194
Number of Sequences: 369166
Number of extensions: 1011850
Number of successful extensions: 2892
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2697
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2858
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4308286840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)