Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_021_F21
(645 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O95197|RTN3_HUMAN Reticulon-3 (Neuroendocrine-specific p... 76 8e-14
sp|Q9ES97|RTN3_MOUSE Reticulon-3 76 8e-14
sp|Q16799|RTN1_HUMAN Reticulon-1 (Neuroendocrine-specific p... 75 1e-13
sp|Q5IS59|RTN1_PANTR Reticulon-1 75 1e-13
sp|Q64548|RTN1_RAT Reticulon-1 (Neuroendocrine-specific pro... 74 3e-13
sp|Q8K0T0|RTN1_MOUSE Reticulon-1 (Neuroendocrine-specific p... 74 3e-13
sp|Q99P72|RTN4_MOUSE Reticulon-4 (Neurite outgrowth inhibit... 71 2e-12
sp|Q9NQC3|RTN4_HUMAN Reticulon-4 (Neurite outgrowth inhibit... 70 4e-12
sp|Q9JK11|RTN4_RAT Reticulon-4 (Neurite outgrowth inhibitor... 70 4e-12
sp|O75298|RTN2_HUMAN Reticulon-2 (Neuroendocrine-specific p... 61 3e-09
>sp|O95197|RTN3_HUMAN Reticulon-3 (Neuroendocrine-specific protein-like 2) (NSP-like
protein II) (NSPLII)
Length = 236
Score = 75.9 bits (185), Expect = 8e-14
Identities = 55/172 (31%), Positives = 84/172 (48%), Gaps = 4/172 (2%)
Frame = +3
Query: 60 TGSILVTWVIFAVMVKTMSFISVIAYIGLILIMSSMLFRFYSNFIN----NEASNPFQEC 227
TG + T +I + + S ISV++Y+ L L+ ++ FR Y + I +E +PF+
Sbjct: 60 TGFVFGTTLIMLLSLAAFSVISVVSYLILALLSVTISFRIYKSVIQAVQKSEEGHPFKAY 119
Query: 228 LDKDVNLYLSKISDFVHFQTSEESSQFKSIIKYLNSIFLLESYADVGKFLVGNVAMVYVG 407
LD D+ L ++++ + K II+ +FL+E D K V M YVG
Sbjct: 120 LDVDITLSSEAFHNYMNAAMVHINRALKLIIR----LFLVEDLVDSLKLAVFMWLMTYVG 175
Query: 408 MYFNLIDLVTLAVLSLLTGPKVYNLYRRQIDDAVMKAREHVGKAVAIAKEKL 563
FN I L+ LA L + + P VY Y+ QID V AR+ V + KL
Sbjct: 176 AVFNGITLLILAELLIFSVPIVYEKYKTQIDHYVGIARDQTKSIVEKIQAKL 227
>sp|Q9ES97|RTN3_MOUSE Reticulon-3
Length = 237
Score = 75.9 bits (185), Expect = 8e-14
Identities = 55/172 (31%), Positives = 84/172 (48%), Gaps = 4/172 (2%)
Frame = +3
Query: 60 TGSILVTWVIFAVMVKTMSFISVIAYIGLILIMSSMLFRFYSNFIN----NEASNPFQEC 227
TG + T +I + + S ISV++Y+ L L+ ++ FR Y + I +E +PF+
Sbjct: 61 TGFVFGTTLIMLLSLAAFSVISVVSYLILALLSVTISFRVYKSVIQAVQKSEEGHPFKAY 120
Query: 228 LDKDVNLYLSKISDFVHFQTSEESSQFKSIIKYLNSIFLLESYADVGKFLVGNVAMVYVG 407
LD D+ L ++++ + K II+ +FL+E D K V M YVG
Sbjct: 121 LDVDITLSSEAFHNYMNAAMVHVNKALKLIIR----LFLVEDLVDSLKLAVFMWLMTYVG 176
Query: 408 MYFNLIDLVTLAVLSLLTGPKVYNLYRRQIDDAVMKAREHVGKAVAIAKEKL 563
FN I L+ LA L + + P VY Y+ QID V AR+ V + KL
Sbjct: 177 AVFNGITLLILAELLVFSVPIVYEKYKTQIDHYVGIARDQTKSIVEKIQAKL 228
>sp|Q16799|RTN1_HUMAN Reticulon-1 (Neuroendocrine-specific protein)
Length = 776
Score = 75.5 bits (184), Expect = 1e-13
Identities = 48/175 (27%), Positives = 89/175 (50%), Gaps = 7/175 (4%)
Frame = +3
Query: 60 TGSILVTWVIFAVMVKTMSFISVIAYIGLILIMSSMLFRFYSNFIN----NEASNPFQEC 227
TG + ++++ + S +SV+AY+ L + +++ FR Y + + + +PF+
Sbjct: 601 TGIVFGSFLLLLFSLTQFSVVSVVAYLALAALSATISFRIYKSVLQAVQKTDEGHPFKAY 660
Query: 228 LDKDVNL---YLSKISDFVHFQTSEESSQFKSIIKYLNSIFLLESYADVGKFLVGNVAMV 398
L+ ++ L + K +D + F + S +K L +FL++ D KF V +
Sbjct: 661 LELEITLSQEQIQKYTDCLQFYVN-------STLKELRRLFLVQDLVDSLKFAVLMWLLT 713
Query: 399 YVGMYFNLIDLVTLAVLSLLTGPKVYNLYRRQIDDAVMKAREHVGKAVAIAKEKL 563
YVG FN + L+ +AV+S+ T P VY ++ QID + R H+ VA + K+
Sbjct: 714 YVGALFNGLTLLLMAVVSMFTLPVVYVKHQAQIDQYLGLVRTHINAVVAKIQAKI 768
>sp|Q5IS59|RTN1_PANTR Reticulon-1
Length = 776
Score = 75.5 bits (184), Expect = 1e-13
Identities = 48/175 (27%), Positives = 89/175 (50%), Gaps = 7/175 (4%)
Frame = +3
Query: 60 TGSILVTWVIFAVMVKTMSFISVIAYIGLILIMSSMLFRFYSNFIN----NEASNPFQEC 227
TG + ++++ + S +SV+AY+ L + +++ FR Y + + + +PF+
Sbjct: 601 TGIVFGSFLLLLFSLTQFSVVSVVAYLALAALSATISFRIYKSVLQAVQKTDEGHPFKAY 660
Query: 228 LDKDVNL---YLSKISDFVHFQTSEESSQFKSIIKYLNSIFLLESYADVGKFLVGNVAMV 398
L+ ++ L + K +D + F + S +K L +FL++ D KF V +
Sbjct: 661 LELEITLSQEQIQKYTDCLQFYVN-------STLKELRRLFLVQDLVDSLKFAVLMWLLT 713
Query: 399 YVGMYFNLIDLVTLAVLSLLTGPKVYNLYRRQIDDAVMKAREHVGKAVAIAKEKL 563
YVG FN + L+ +AV+S+ T P VY ++ QID + R H+ VA + K+
Sbjct: 714 YVGALFNGLTLLLMAVVSMFTLPVVYVKHQAQIDQYLGLVRTHINAVVAKIQAKI 768
>sp|Q64548|RTN1_RAT Reticulon-1 (Neuroendocrine-specific protein) (S-rex)
Length = 777
Score = 73.9 bits (180), Expect = 3e-13
Identities = 46/172 (26%), Positives = 86/172 (50%), Gaps = 4/172 (2%)
Frame = +3
Query: 60 TGSILVTWVIFAVMVKTMSFISVIAYIGLILIMSSMLFRFYSNFIN----NEASNPFQEC 227
TG + ++++ + S +SV+AY+ L + +++ FR Y + + + +PF+
Sbjct: 602 TGIVFGSFLLLLFSLTQFSVVSVVAYLALAALSATISFRIYKSVLQAVQKTDEGHPFKAY 661
Query: 228 LDKDVNLYLSKISDFVHFQTSEESSQFKSIIKYLNSIFLLESYADVGKFLVGNVAMVYVG 407
L+ ++ L +I + T S +K L +FL++ D KF V + YVG
Sbjct: 662 LELEITLSQEQIQKY----TDCLQLYVNSTLKELRRLFLVQDLVDSLKFAVLMWLLTYVG 717
Query: 408 MYFNLIDLVTLAVLSLLTGPKVYNLYRRQIDDAVMKAREHVGKAVAIAKEKL 563
FN + L+ +AV+S+ T P VY ++ Q+D + R H+ VA + K+
Sbjct: 718 ALFNGLTLLLMAVVSMFTLPVVYVKHQAQVDQYLGLVRTHINTVVAKIQAKI 769
>sp|Q8K0T0|RTN1_MOUSE Reticulon-1 (Neuroendocrine-specific protein)
Length = 780
Score = 73.9 bits (180), Expect = 3e-13
Identities = 46/172 (26%), Positives = 86/172 (50%), Gaps = 4/172 (2%)
Frame = +3
Query: 60 TGSILVTWVIFAVMVKTMSFISVIAYIGLILIMSSMLFRFYSNFIN----NEASNPFQEC 227
TG + ++++ + S +SV+AY+ L + +++ FR Y + + + +PF+
Sbjct: 605 TGIVFGSFLLLLFSLTQFSVVSVVAYLALAALSATISFRIYKSVLQAVQKTDEGHPFKAY 664
Query: 228 LDKDVNLYLSKISDFVHFQTSEESSQFKSIIKYLNSIFLLESYADVGKFLVGNVAMVYVG 407
L+ ++ L +I + T S +K L +FL++ D KF V + YVG
Sbjct: 665 LELEITLSQEQIQKY----TDCLQLYVNSTLKELRRLFLVQDLVDSLKFAVLMWLLTYVG 720
Query: 408 MYFNLIDLVTLAVLSLLTGPKVYNLYRRQIDDAVMKAREHVGKAVAIAKEKL 563
FN + L+ +AV+S+ T P VY ++ Q+D + R H+ VA + K+
Sbjct: 721 ALFNGLTLLLMAVVSMFTLPVVYVKHQAQVDQYLGLVRTHINTVVAKIQAKI 772
>sp|Q99P72|RTN4_MOUSE Reticulon-4 (Neurite outgrowth inhibitor) (Nogo protein)
Length = 199
Score = 71.2 bits (173), Expect = 2e-12
Identities = 50/175 (28%), Positives = 84/175 (48%), Gaps = 4/175 (2%)
Frame = +3
Query: 60 TGSILVTWVIFAVMVKTMSFISVIAYIGLILIMSSMLFRFYSNFIN----NEASNPFQEC 227
TG + + + + S +SV AYI L L+ ++ FR Y I ++ +PF+
Sbjct: 24 TGVVFGASLFLLLSLTVFSIVSVTAYIALALLSVTISFRIYKGVIQAIQKSDEGHPFRAY 83
Query: 228 LDKDVNLYLSKISDFVHFQTSEESSQFKSIIKYLNSIFLLESYADVGKFLVGNVAMVYVG 407
L+ +V + + V ++ S IK L +FL++ D KF V YVG
Sbjct: 84 LESEVAIS----EELVQKYSNSALGHVNSTIKELRRLFLVDDLVDSLKFAVLMWVFTYVG 139
Query: 408 MYFNLIDLVTLAVLSLLTGPKVYNLYRRQIDDAVMKAREHVGKAVAIAKEKLATL 572
FN + L+ LA++SL + P +Y ++ QID + A + V A+A + K+ L
Sbjct: 140 ALFNGLTLLILALISLFSIPVIYERHQAQIDHYLGLANKSVKDAMAKIQAKIPGL 194
>sp|Q9NQC3|RTN4_HUMAN Reticulon-4 (Neurite outgrowth inhibitor) (Nogo protein) (Foocen)
(Neuroendocrine-specific protein) (NSP)
(Neuroendocrine-specific protein C homolog) (RTN-x)
(Reticulon-5)
Length = 1192
Score = 70.5 bits (171), Expect = 4e-12
Identities = 49/175 (28%), Positives = 84/175 (48%), Gaps = 4/175 (2%)
Frame = +3
Query: 60 TGSILVTWVIFAVMVKTMSFISVIAYIGLILIMSSMLFRFYSNFIN----NEASNPFQEC 227
TG + + + + S +SV AYI L L+ ++ FR Y I ++ +PF+
Sbjct: 1017 TGVVFGASLFLLLSLTVFSIVSVTAYIALALLSVTISFRIYKGVIQAIQKSDEGHPFRAY 1076
Query: 228 LDKDVNLYLSKISDFVHFQTSEESSQFKSIIKYLNSIFLLESYADVGKFLVGNVAMVYVG 407
L+ +V + + V ++ IK L +FL++ D KF V YVG
Sbjct: 1077 LESEVAIS----EELVQKYSNSALGHVNCTIKELRRLFLVDDLVDSLKFAVLMWVFTYVG 1132
Query: 408 MYFNLIDLVTLAVLSLLTGPKVYNLYRRQIDDAVMKAREHVGKAVAIAKEKLATL 572
FN + L+ LA++SL + P +Y ++ QID + A ++V A+A + K+ L
Sbjct: 1133 ALFNGLTLLILALISLFSVPVIYERHQAQIDHYLGLANKNVKDAMAKIQAKIPGL 1187
>sp|Q9JK11|RTN4_RAT Reticulon-4 (Neurite outgrowth inhibitor) (Nogo protein) (Foocen)
(Glut4 vesicle 20 kDa protein)
Length = 1163
Score = 70.5 bits (171), Expect = 4e-12
Identities = 50/175 (28%), Positives = 84/175 (48%), Gaps = 4/175 (2%)
Frame = +3
Query: 60 TGSILVTWVIFAVMVKTMSFISVIAYIGLILIMSSMLFRFYSNFIN----NEASNPFQEC 227
TG + + + + S +SV AYI L L+ ++ FR Y I ++ +PF+
Sbjct: 988 TGVVFGASLFLLLSLTVFSIVSVTAYIALALLSVTISFRIYKGVIQAIQKSDEGHPFRAY 1047
Query: 228 LDKDVNLYLSKISDFVHFQTSEESSQFKSIIKYLNSIFLLESYADVGKFLVGNVAMVYVG 407
L+ +V + + V ++ S IK L +FL++ D KF V YVG
Sbjct: 1048 LESEVAIS----EELVQKYSNSALGHVNSTIKELRRLFLVDDLVDSLKFAVLMWVFTYVG 1103
Query: 408 MYFNLIDLVTLAVLSLLTGPKVYNLYRRQIDDAVMKAREHVGKAVAIAKEKLATL 572
FN + L+ LA++SL + P +Y ++ QID + A + V A+A + K+ L
Sbjct: 1104 ALFNGLTLLILALISLFSIPVIYERHQVQIDHYLGLANKSVKDAMAKIQAKIPGL 1158
>sp|O75298|RTN2_HUMAN Reticulon-2 (Neuroendocrine-specific protein-like 1) (NSP-like
protein 1) (NSPLI)
Length = 545
Score = 60.8 bits (146), Expect = 3e-09
Identities = 41/154 (26%), Positives = 72/154 (46%), Gaps = 4/154 (2%)
Frame = +3
Query: 60 TGSILVTWVIFAVMVKTMSFISVIAYIGLILIMSSMLFRFYSNFIN----NEASNPFQEC 227
+G + ++ + + S +SV A++ L+L+ ++ R Y + + +NPFQ
Sbjct: 357 SGVVFTGLMVSLLCLLHFSIVSVAAHLALLLLCGTISLRVYRKVLQAVHRGDGANPFQAY 416
Query: 228 LDKDVNLYLSKISDFVHFQTSEESSQFKSIIKYLNSIFLLESYADVGKFLVGNVAMVYVG 407
LD D+ L + H + +S+ S L FL+E D K + + +VG
Sbjct: 417 LDVDLTLTREQTERLSH----QITSRVVSAATQLRHFFLVEDLVDSLKLALLFYILTFVG 472
Query: 408 MYFNLIDLVTLAVLSLLTGPKVYNLYRRQIDDAV 509
FN + L+ L V+ L T P +Y ++ QID V
Sbjct: 473 AIFNGLTLLILGVIGLFTIPLLYRQHQAQIDQYV 506
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 74,762,921
Number of Sequences: 369166
Number of extensions: 1482667
Number of successful extensions: 4673
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4513
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4663
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 5267491560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)