Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_021_E11
(847 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P32596|VGLN_BEFV Nonstructural glycoprotein GNS precursor 35 0.34
sp|Q5FIX8|Y1527_LACAC Hypothetical UPF0348 protein LBA1527 33 0.76
sp|P33893|GATB_YEAST Probable glutamyl-tRNA(Gln) amidotrans... 32 2.2
sp|P36431|SYP_CHLTR Prolyl-tRNA synthetase (Proline--tRNA l... 32 2.9
sp|Q9PK01|SYP_CHLMU Prolyl-tRNA synthetase (Proline--tRNA l... 32 2.9
sp|P26361|CFTR_MOUSE Cystic fibrosis transmembrane conducta... 30 6.4
sp|P22119|VP61_MRDV Probable nonstructural 41.0 kDa protein 30 8.4
sp|Q03153|YMY8_YEAST Hypothetical 70.4 kDa protein in SNZ1-... 30 8.4
sp|Q9Z614|SYK3_BUCAP Putative lysyl-tRNA synthetase (Lysine... 30 8.4
sp|P56600|GAL10_CANMA GAL10 bifunctional protein [Includes:... 30 8.4
>sp|P32596|VGLN_BEFV Nonstructural glycoprotein GNS precursor
Length = 586
Score = 34.7 bits (78), Expect = 0.34
Identities = 60/261 (22%), Positives = 107/261 (40%), Gaps = 7/261 (2%)
Frame = +3
Query: 3 NISESEFPLFLYNEMRYLNIEPTLGTFSLLLQCKFSRKYYFYLVKDIIEILEKRREE--N 176
N S E P + RY N+ P L T + F L K I + LE ++ N
Sbjct: 274 NRSRIEMPETNLAKKRYSNVGPVLLT---------TLNKRFELCKKIKKNLELKQPIPIN 324
Query: 177 IFEELTSEDDGKLFANLSEIIYSLMSLELNQRLHNLAIQGGNLPLLGSSSDWGKYIKNFS 356
L + GK A ++Y L+S +NQ L N + L + G+ I++
Sbjct: 325 NLHYLAPFEPGKHPA----LVYRLVSTTINQSLRNKVVPVSMLSMSMCEYITGQIIEDGI 380
Query: 357 KVLIKNEPNLGVVFSNLKIWVPYYFHLNNVSVELLIDNIASTSSTHFIPDLINSVHWFSQ 536
K + +E + ++ +N +I N E NI + H S +W++
Sbjct: 381 KRNLTDEDTVIILANNKEIKWKDLKGRENWYQEQANPNIIDKNPDHL------SYYWYNG 434
Query: 537 NITKSDLFHRLLQLILKYV-DFSTETELKSRIIDSFHRVFEMMT----IEYRFVMEPQIV 701
+ + D F + IL+ + T+TE +SRI R+ +T I++R + E
Sbjct: 435 VMRREDKFTYPSRYILQTLKKIYTDTERESRISFFKFRLERNITKTEVIKFRDI-EESSD 493
Query: 702 PPFVELLRRIDDGDLFWKYID 764
+ + + +GD +W +++
Sbjct: 494 QDHSQSVNKTLEGDDYWNWVE 514
>sp|Q5FIX8|Y1527_LACAC Hypothetical UPF0348 protein LBA1527
Length = 383
Score = 33.5 bits (75), Expect = 0.76
Identities = 33/116 (28%), Positives = 49/116 (42%), Gaps = 7/116 (6%)
Frame = +3
Query: 504 DLINSVHWFSQN----ITKSDLFHR--LLQLILKYVDFSTETELKSRIIDSFHRVFEMMT 665
D N +W +N + K ++ LL LKY ++S I+ R+++M
Sbjct: 208 DTSNLKYWMPKNEANILAKQKVYPNWNLLYPFLKY-------RIESSSIEDLRRIYQMSE 260
Query: 666 -IEYRFVMEPQIVPPFVELLRRIDDGDLFWKYIDYMCQRGLVLPTLLNELIERLHD 830
+EY+ E + F E LRRI K Y R L L TLLN + + D
Sbjct: 261 GLEYKMKQEIHLARDFTEFLRRIKS-----KRYTYARLRRLSLYTLLNVTYDDMLD 311
>sp|P33893|GATB_YEAST Probable glutamyl-tRNA(Gln) amidotransferase subunit B,
mitochondrial precursor (GLU-ADT subunit B) (Cytochrome
oxidase assembly factor PET112)
Length = 541
Score = 32.0 bits (71), Expect = 2.2
Identities = 24/113 (21%), Positives = 47/113 (41%)
Frame = +3
Query: 72 LGTFSLLLQCKFSRKYYFYLVKDIIEILEKRREENIFEELTSEDDGKLFANLSEIIYSLM 251
+G L ++ + +Y KD+I +++ R EL ++ D + I
Sbjct: 148 IGILQLQIEQDTGKSHYTETDKDVITLVDLNRSNVPLIELVTKPDFSDIKQVRAFIKKYQ 207
Query: 252 SLELNQRLHNLAIQGGNLPLLGSSSDWGKYIKNFSKVLIKNEPNLGVVFSNLK 410
+L + +L I G+L D I +++V +KN PN + + +K
Sbjct: 208 NL-----VRHLHISSGDLETGAMRVDVNLSINEYARVELKNLPNTSSIINAIK 255
>sp|P36431|SYP_CHLTR Prolyl-tRNA synthetase (Proline--tRNA ligase) (ProRS)
Length = 581
Score = 31.6 bits (70), Expect = 2.9
Identities = 15/43 (34%), Positives = 23/43 (53%)
Frame = -3
Query: 758 IFPEQITIIDSPQQLHERRNNLRFHDESIFDRHHFKYPMERID 630
+ + T DSP+Q+ E+ LR + IFDR + KY + D
Sbjct: 158 LMEDSYTFSDSPEQMEEQYAKLRLAYQRIFDRLNLKYVIVAAD 200
>sp|Q9PK01|SYP_CHLMU Prolyl-tRNA synthetase (Proline--tRNA ligase) (ProRS)
Length = 579
Score = 31.6 bits (70), Expect = 2.9
Identities = 15/43 (34%), Positives = 23/43 (53%)
Frame = -3
Query: 758 IFPEQITIIDSPQQLHERRNNLRFHDESIFDRHHFKYPMERID 630
+ + T DSP+Q+ E+ LR + IFDR + KY + D
Sbjct: 158 LMEDSYTFSDSPEQMEEQYAKLRLAYQRIFDRLNLKYVIVTAD 200
>sp|P26361|CFTR_MOUSE Cystic fibrosis transmembrane conductance regulator (CFTR)
(cAMP-dependent chloride channel)
Length = 1476
Score = 30.4 bits (67), Expect = 6.4
Identities = 20/89 (22%), Positives = 44/89 (49%), Gaps = 2/89 (2%)
Frame = +3
Query: 504 DLINSVHWFSQNITKSDLFHRLLQLILKYVDFSTETELKSRIIDSFHR--VFEMMTIEYR 677
++++++ W + +D R + + K++D TE + ++II R +++ I+
Sbjct: 1133 NIMSTLQWAVNSSIDTDSLMRSVSRVFKFIDIQTEESMYTQIIKELpreGSSDVLVIKNE 1192
Query: 678 FVMEPQIVPPFVELLRRIDDGDLFWKYID 764
V + I P E++ + DL KY+D
Sbjct: 1193 HVKKSDIWPSGGEMVVK----DLTVKYMD 1217
>sp|P22119|VP61_MRDV Probable nonstructural 41.0 kDa protein
Length = 362
Score = 30.0 bits (66), Expect = 8.4
Identities = 24/90 (26%), Positives = 42/90 (46%), Gaps = 1/90 (1%)
Frame = +3
Query: 324 SDWGKYIKNFSKVLIKNEPNLGVVFSNLKIWVPYYFHLNNVSVELLIDNIASTSSTH-FI 500
SD +++ +K++IKN+ + V + + + YYF + + L D+ +S S H +
Sbjct: 147 SDALRFLARIAKIVIKNDVDKSDVVAAQQTLIYYYFGNSYQGIHLNWDSKSSQQSIHGYS 206
Query: 501 PDLINSVHWFSQNITKSDLFHRLLQLILKY 590
+ H+ K DLFH L L Y
Sbjct: 207 TSEVCLDHYIRM---KVDLFHGLRSKNLVY 233
>sp|Q03153|YMY8_YEAST Hypothetical 70.4 kDa protein in SNZ1-YPK2 intergenic region
Length = 612
Score = 30.0 bits (66), Expect = 8.4
Identities = 20/63 (31%), Positives = 33/63 (52%)
Frame = +3
Query: 63 EPTLGTFSLLLQCKFSRKYYFYLVKDIIEILEKRREENIFEELTSEDDGKLFANLSEIIY 242
E + ++L+ + S YF LVK I+ R N+F+ L +EDDG + E++
Sbjct: 416 EDLIALLEMVLKTQHSSHSYFNLVKQNRVII---RALNLFKGLQTEDDGSVV--YDEVVI 470
Query: 243 SLM 251
SL+
Sbjct: 471 SLL 473
>sp|Q9Z614|SYK3_BUCAP Putative lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS) (GX)
Length = 324
Score = 30.0 bits (66), Expect = 8.4
Identities = 15/40 (37%), Positives = 24/40 (60%), Gaps = 2/40 (5%)
Frame = +3
Query: 45 MRYLNIEPTLGTFSLLLQC--KFSRKYYFYLVKDIIEILE 158
M++L I+P + S L QC KF+ K YL D+ +++E
Sbjct: 153 MKFLKIDPLSTSLSELFQCYKKFNLKNLIYLENDLNQLIE 192
>sp|P56600|GAL10_CANMA GAL10 bifunctional protein [Includes: UDP-glucose 4-epimerase
(Galactowaldenase); Aldose 1-epimerase (Mutarotase)]
Length = 153
Score = 30.0 bits (66), Expect = 8.4
Identities = 20/71 (28%), Positives = 34/71 (47%), Gaps = 16/71 (22%)
Frame = +3
Query: 336 KYIKNFSKVLIKNEPNLGVVFSNLKI----------------WVPYYFHLNNVSVELLID 467
+++ +F KV I+NE L VFSN KI +P ++ NNVS + +
Sbjct: 53 QHVSHFIKVDIRNEKELNQVFSNHKISGVIHFAALKAVGESTKIPLEYYDNNVSGTIALL 112
Query: 468 NIASTSSTHFI 500
N+ +++ I
Sbjct: 113 NVCKSNNVKTI 123
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 100,412,400
Number of Sequences: 369166
Number of extensions: 2156211
Number of successful extensions: 6555
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6266
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6548
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8293644780
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)