Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_021_E09
(872 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P56287|EI2BE_SCHPO Probable translation initiation facto... 32 2.3
sp|P29098|RRPO_PMMVS RNA-directed RNA polymerase (183 kDa p... 30 6.7
sp|Q8NEV8|SLC2B_HUMAN Slp homolog lacking C2 domains-b (Exo... 30 6.7
sp|P89657|RRPO_PMMVJ RNA-directed RNA polymerase (183 kDa p... 30 8.8
>sp|P56287|EI2BE_SCHPO Probable translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor)
Length = 678
Score = 32.0 bits (71), Expect = 2.3
Identities = 21/48 (43%), Positives = 28/48 (58%), Gaps = 2/48 (4%)
Frame = +3
Query: 105 FNENESLQLISELAEIDCNFSICSNN--TLNEIHFQYWSIRNSKDIGY 242
FNE+E L++ ++L IDC ICSN+ L +F Y IR KD Y
Sbjct: 206 FNEHEELEVRNDL--IDCQIDICSNDVPALFTENFDYQDIR--KDFVY 249
>sp|P29098|RRPO_PMMVS RNA-directed RNA polymerase (183 kDa protein) [Contains:
Methyltransferase/RNA helicase (MT/HEL) (126 kDa
protein)]
Length = 1612
Score = 30.4 bits (67), Expect = 6.7
Identities = 61/287 (21%), Positives = 101/287 (35%), Gaps = 31/287 (10%)
Frame = +3
Query: 90 CQLVRFNENESLQLISELAEIDCNFSICSNNTLNEIHFQYWSIRNSKDIGYMSYFRSFTV 269
CQ R +E L L S+CS F Y +++ I Y++ +F
Sbjct: 899 CQYKRLFLDEGLMLHPGCVNFLVGMSLCSEA------FVYG---DTQQIPYINRVATFPY 949
Query: 270 PTQQKILR------NRNCLPCSKDLRLFSTSFQRFQ--CTSFIPSSCQSPIFQQP---NC 416
P L R L C D+ F Q CTS + S + Q N
Sbjct: 950 PKHLSQLEVDAVETRRTTLRCPADITFFLNQKYEGQVMCTSSVTRSVSHEVIQGAAVMNP 1009
Query: 417 FKEPVK----QIMNAENQILQSKYFAFPQQMIKQTGNLF-----VRSIENQFRFVQCREN 569
+P+K ++ +L S+ + + + G F VR + +
Sbjct: 1010 VSKPLKGKVITFTQSDKSLLLSRGYEDVHTVHEVQGETFEDVSLVRLTPTPVGIISKQSP 1069
Query: 570 DLLDSLYRYFLDILICSIVHKHFQIDIK----TNRYLLDQLQLDSSRRKHDGNNVFHKSV 737
LL SL R+ I ++V ++ + YLLD ++D S + +K V
Sbjct: 1070 HLLVSLSRHTRSIKYYTVVLDAVVSVLRDLECVSSYLLDMYKVDVSTQXQLQIESVYKGV 1129
Query: 738 NLF-------PQQKLQFYKDPRFDAFGEVVKKISLDYDKMRREQRDN 857
NLF +Q+Y D ++ +YD + + R+N
Sbjct: 1130 NLFVAAPKTGDVSDMQYYYDKCLPGNSTILN----EYDAVTMQIREN 1172
>sp|Q8NEV8|SLC2B_HUMAN Slp homolog lacking C2 domains-b (Exophilin-5)
Length = 1989
Score = 30.4 bits (67), Expect = 6.7
Identities = 20/50 (40%), Positives = 27/50 (54%), Gaps = 1/50 (2%)
Frame = +1
Query: 139 NSLRSTAISRSAPTTLSMKSISNIGRSETRKISVTCRISA-ALPFRHNRK 285
N +S S S P++L S NIG S+TR+ S C S A+PF + K
Sbjct: 1412 NCQQSINSSNSGPSSLPALSEVNIGNSQTRRSSWECTGSGRAIPFTGSGK 1461
>sp|P89657|RRPO_PMMVJ RNA-directed RNA polymerase (183 kDa protein) [Contains:
Methyltransferase/RNA helicase (MT/HEL) (126 kDa
protein)]
Length = 1612
Score = 30.0 bits (66), Expect = 8.8
Identities = 61/287 (21%), Positives = 101/287 (35%), Gaps = 31/287 (10%)
Frame = +3
Query: 90 CQLVRFNENESLQLISELAEIDCNFSICSNNTLNEIHFQYWSIRNSKDIGYMSYFRSFTV 269
CQ R +E L L S+CS F Y +++ I Y++ +F
Sbjct: 899 CQYKRLFLDEGLMLHPGCVNFLVGMSLCSEA------FVYG---DTQQIPYINRVATFPY 949
Query: 270 PTQQKILR------NRNCLPCSKDLRLFSTSFQRFQ--CTSFIPSSCQSPIFQQP---NC 416
P L R L C D+ F Q CTS + S + Q N
Sbjct: 950 PKHLSQLEVDAVETRRTTLRCPADITFFLNQKYEGQVMCTSSVTRSVSHEVIQGAAVMNP 1009
Query: 417 FKEPVK----QIMNAENQILQSKYFAFPQQMIKQTGNLF-----VRSIENQFRFVQCREN 569
+P+K ++ +L S+ + + + G F VR + +
Sbjct: 1010 VSKPLKGKVITFTQSDKSLLLSRGYEDVHTVHEVQGETFEDVSLVRLTPTPVGIISKQSP 1069
Query: 570 DLLDSLYRYFLDILICSIVHKHFQIDIK----TNRYLLDQLQLDSSRRKHDGNNVFHKSV 737
LL SL R+ I ++V ++ + YLLD ++D S + +K V
Sbjct: 1070 HLLVSLSRHTRSIKYYTVVLDAVVSVLRDLECVSSYLLDMYKVDVSTQYQLQIESVYKGV 1129
Query: 738 NLF-------PQQKLQFYKDPRFDAFGEVVKKISLDYDKMRREQRDN 857
NLF +Q+Y D ++ +YD + + R+N
Sbjct: 1130 NLFVAAPKTGDVSDMQYYYDKCLPGNSTILN----EYDAVTMQIREN 1172
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 96,128,083
Number of Sequences: 369166
Number of extensions: 1921727
Number of successful extensions: 6116
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 5886
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6113
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8646143400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)