Planarian EST Database


Dr_sW_021_E08

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_021_E08
         (736 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P42932|TCPQ_MOUSE  T-complex protein 1, theta subunit (TC...   199   6e-51
sp|P50990|TCPQ_HUMAN  T-complex protein 1, theta subunit (TC...   196   7e-50
sp|P47828|TCPQ_CANAL  T-complex protein 1, theta subunit (TC...   149   8e-36
sp|P78921|TCPQ_SCHPO  Probable T-complex protein 1, theta su...   140   5e-33
sp|P47079|TCPQ_YEAST  T-complex protein 1, theta subunit (TC...   137   2e-32
sp|Q58405|THS_METJA  Thermosome subunit (Chaperonin subunit)      113   5e-25
sp|O26885|THSB_METTH  Thermosome beta subunit (Thermosome su...   109   7e-24
sp|Q9HN70|THSA_HALSA  Thermosome alpha subunit (Thermosome s...   107   3e-23
sp|Q9YDK6|THSA_AERPE  Thermosome alpha subunit (Thermosome s...   107   4e-23
sp|O93624|THS_METTL  Thermosome subunit (Chaperonin subunit)      105   1e-22
>sp|P42932|TCPQ_MOUSE T-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
          Length = 548

 Score =  199 bits (506), Expect = 6e-51
 Identities = 100/198 (50%), Positives = 139/198 (70%)
 Frame = +2

Query: 8   GATPIPKLSTPTQEELGYIASVRTEEIGKSTVVLFDQDVKEGAISSIVVRGSMYDMMDEV 187
           GAT +PKL+ P QEE+G+  SV   E+G + VV+F  + ++GAIS+IV+RGS  ++MD++
Sbjct: 329 GATALPKLTPPVQEEMGHCDSVYLSEVGDTQVVVFKHEKEDGAISTIVLRGSTDNLMDDI 388

Query: 188 ERAIDDGVNTFKCLTKEPRYLAGAGATEIELANRLSDFANTQTGLVQYAIKQFVLAFESI 367
           ERA+DDGVNTFK LT++ R + G GATEIELA +++ +  T  GL QYAIK+F  AFE+I
Sbjct: 389 ERAVDDGVNTFKVLTRDKRLVPGGGATEIELAKQITSYGETCPGLEQYAIKKFAEAFEAI 448

Query: 368 VKVLCDNAGENATERIAELKAHHREGKTTFGIISTSEGRKVDDAVEHQILDTYTAKYWGI 547
            + L +N+G  A E I++L + H+EG    G+   +E   V D +E  ILDTY  KYW I
Sbjct: 449 PRALAENSGVKANEVISKLYSVHQEGNKNVGLDIEAEVPAVKDMLEASILDTYLGKYWAI 508

Query: 548 RFAADTAVTILRVDNIIM 601
           + A + AVT+LRVD IIM
Sbjct: 509 KLATNAAVTVLRVDQIIM 526
>sp|P50990|TCPQ_HUMAN T-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
 sp|Q5RAP1|TCPQ_PONPY T-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
          Length = 548

 Score =  196 bits (497), Expect = 7e-50
 Identities = 99/198 (50%), Positives = 138/198 (69%)
 Frame = +2

Query: 8   GATPIPKLSTPTQEELGYIASVRTEEIGKSTVVLFDQDVKEGAISSIVVRGSMYDMMDEV 187
           GAT +P+L+ P  EE+G+  SV   E+G + VV+F  + ++GAIS+IV+RGS  ++MD++
Sbjct: 329 GATALPRLTPPVLEEMGHCDSVYLSEVGDTQVVVFKHEKEDGAISTIVLRGSTDNLMDDI 388

Query: 188 ERAIDDGVNTFKCLTKEPRYLAGAGATEIELANRLSDFANTQTGLVQYAIKQFVLAFESI 367
           ERA+DDGVNTFK LT++ R + G GATEIELA +++ +  T  GL QYAIK+F  AFE+I
Sbjct: 389 ERAVDDGVNTFKVLTRDKRLVPGGGATEIELAKQITSYGETCPGLEQYAIKKFAEAFEAI 448

Query: 368 VKVLCDNAGENATERIAELKAHHREGKTTFGIISTSEGRKVDDAVEHQILDTYTAKYWGI 547
            + L +N+G  A E I++L A H+EG    G+   +E   V D +E  ILDTY  KYW I
Sbjct: 449 PRALAENSGVKANEVISKLYAVHQEGNKNVGLDIEAEVPAVKDMLEAGILDTYLGKYWAI 508

Query: 548 RFAADTAVTILRVDNIIM 601
           + A + AVT+LRVD IIM
Sbjct: 509 KLATNAAVTVLRVDQIIM 526
>sp|P47828|TCPQ_CANAL T-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
          Length = 540

 Score =  149 bits (376), Expect = 8e-36
 Identities = 87/200 (43%), Positives = 118/200 (59%)
 Frame = +2

Query: 2   VTGATPIPKLSTPTQEELGYIASVRTEEIGKSTVVLFDQDVKEGAISSIVVRGSMYDMMD 181
           V GATP+P+L  PT +E+G I  + T+EIG   V +F QD      ++IVVRG+  + +D
Sbjct: 324 VCGATPLPRLGAPTPDEMGEIDIIETKEIGGDRVTIFRQDESSSRTATIVVRGATQNNLD 383

Query: 182 EVERAIDDGVNTFKCLTKEPRYLAGAGATEIELANRLSDFANTQTGLVQYAIKQFVLAFE 361
           ++ERAIDDGVN+ K L K+ R L GAGA EIEL  R++ + +T  GL+Q AIK F  AFE
Sbjct: 384 DIERAIDDGVNSIKGLIKDNRLLPGAGAVEIELMKRITAYQST-PGLLQLAIKSFAKAFE 442

Query: 362 SIVKVLCDNAGENATERIAELKAHHREGKTTFGIISTSEGRKVDDAVEHQILDTYTAKYW 541
            I +VL + +G +++E +++L A H E       I    G   D AV    LD    K  
Sbjct: 443 VIPRVLAETSGHDSSEILSKLHAAHAEDTGLRAGIDIDSGEVADTAV----LDILATKKS 498

Query: 542 GIRFAADTAVTILRVDNIIM 601
            I  A D   TIL +D IIM
Sbjct: 499 AIDLAVDATNTILSIDQIIM 518
>sp|P78921|TCPQ_SCHPO Probable T-complex protein 1, theta subunit (TCP-1-theta)
           (CCT-theta)
          Length = 546

 Score =  140 bits (352), Expect = 5e-33
 Identities = 79/201 (39%), Positives = 117/201 (58%), Gaps = 1/201 (0%)
 Frame = +2

Query: 2   VTGATPIPKLSTPTQEELGYIASVRTEEIGKSTVVLFDQDVKEGAISSIVVRGSMYDMMD 181
           V GATP+ ++  P  EE+G +  V T EIG   V +F Q       ++IV+RG+    +D
Sbjct: 327 VVGATPLARMGVPMPEEMGSVDVVETIEIGGDRVTVFRQVEDITRTATIVLRGATKTYLD 386

Query: 182 EVERAIDDGVNTFKCLTKEPRYLAGAGATEIELANRLSDFANTQTGLVQYAIKQFVLAFE 361
           ++ERAIDDGVN  K L K+ R + GAGA++++L  RL        G+ Q+AIKQ+  AFE
Sbjct: 387 DLERAIDDGVNIVKALVKDNRLIFGAGASDMQLCIRLISVGEKTPGIYQHAIKQYGEAFE 446

Query: 362 SIVKVLCDNAGENATERIAEL-KAHHREGKTTFGIISTSEGRKVDDAVEHQILDTYTAKY 538
            + + + +NAG + T+ I++L  AHH+E   + G+    E     DA E  I D   AK 
Sbjct: 447 VVPRTISENAGLDPTDVISKLYAAHHKENGESIGVDVECENDGTLDAKEAGIFDVLLAKK 506

Query: 539 WGIRFAADTAVTILRVDNIIM 601
             IR A +T +T+L VD ++M
Sbjct: 507 SAIRLATETVLTVLNVDQVVM 527
>sp|P47079|TCPQ_YEAST T-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
          Length = 568

 Score =  137 bits (346), Expect = 2e-32
 Identities = 86/214 (40%), Positives = 118/214 (55%), Gaps = 14/214 (6%)
 Frame = +2

Query: 2   VTGATPIPKLSTPTQEELGYIASVRTEEIGKSTVVLFDQDVKE-GAISSIVVRGSMYDMM 178
           V GATP+P+L  PT EELG + +V+T EIG   V +F Q+  E    S+I++RG+  + +
Sbjct: 335 VCGATPLPRLGAPTPEELGLVETVKTMEIGGDRVTVFKQEQGEISRTSTIILRGATQNNL 394

Query: 179 DEVERAIDDGVNTFKCLTKEP--RYLAGAGATEIELANRLSDFANTQTGLVQYAIKQFVL 352
           D++ERAIDDGV   K L K    + L GAGATEIEL +R++ +     GL+Q AIKQF +
Sbjct: 395 DDIERAIDDGVAAVKGLMKPSGGKLLPGAGATEIELISRITKYGERTPGLLQLAIKQFAV 454

Query: 353 AFESIVKVLCDNAGENATERIAELKAHHREGKTTFGIISTSEGRK-----------VDDA 499
           AFE + + L + AG +  E +  L A H    T  G + T    K           V D 
Sbjct: 455 AFEVVPRTLAETAGLDVNEVLPNLYAAH--NVTEPGAVKTDHLYKGVDIDGESDEGVKDI 512

Query: 500 VEHQILDTYTAKYWGIRFAADTAVTILRVDNIIM 601
            E  I D    K + I  A + A T+L +D IIM
Sbjct: 513 REENIYDMLATKKFAINVATEAATTVLSIDQIIM 546
>sp|Q58405|THS_METJA Thermosome subunit (Chaperonin subunit)
          Length = 542

 Score =  113 bits (283), Expect = 5e-25
 Identities = 69/199 (34%), Positives = 111/199 (55%), Gaps = 1/199 (0%)
 Frame = +2

Query: 5   TGATPIPKLSTPTQEELGYIASVRTEEIGKSTVVLFDQDVKEGAISSIVVRGSMYDMMDE 184
           TGA  + K+   T E+LG    V   ++    ++  +Q     A++ I+ RGS   +++E
Sbjct: 325 TGARIVTKIDDLTPEDLGEAGLVEERKVAGDAMIFVEQCKHPKAVT-ILARGSTEHVVEE 383

Query: 185 VERAIDDGVNTFKCLTKEPRYLAGAGATEIELANRLSDFANTQTGLVQYAIKQFVLAFES 364
           V RAIDD +   KC  +E + +AG GATEIELA RL  FA +  G  Q A+K F  A E 
Sbjct: 384 VARAIDDAIGVVKCALEEGKIVAGGGATEIELAKRLRKFAESVAGREQLAVKAFADALEV 443

Query: 365 IVKVLCDNAGENATERIAELK-AHHREGKTTFGIISTSEGRKVDDAVEHQILDTYTAKYW 541
           I + L +N+G +  + + +L+ AH +EG   +G +   EG +V D +E  +++    K  
Sbjct: 444 IPRTLAENSGLDPIDMLVKLRAAHEKEGGEVYG-LDVFEG-EVVDMLEKGVVEPLKVKTQ 501

Query: 542 GIRFAADTAVTILRVDNII 598
            I  A + +V +LR+D++I
Sbjct: 502 AIDSATEASVMLLRIDDVI 520
>sp|O26885|THSB_METTH Thermosome beta subunit (Thermosome subunit 2) (Chaperonin beta
           subunit)
          Length = 538

 Score =  109 bits (273), Expect = 7e-24
 Identities = 66/198 (33%), Positives = 113/198 (57%)
 Frame = +2

Query: 5   TGATPIPKLSTPTQEELGYIASVRTEEIGKSTVVLFDQDVKEGAISSIVVRGSMYDMMDE 184
           TGA  +  +   + E+LG    V  ++I    ++ F ++ KE    +I+VRGS   ++ E
Sbjct: 321 TGANIVTNIEDLSPEDLGEAGVVSEKKISGEEMI-FVEECKEPKAVTILVRGSTEHVVSE 379

Query: 185 VERAIDDGVNTFKCLTKEPRYLAGAGATEIELANRLSDFANTQTGLVQYAIKQFVLAFES 364
           VERAI+D +       ++ + +AG GA EIE+A RL D+A++ +G  Q A+  F  A E 
Sbjct: 380 VERAIEDAIGVVAATVEDGKVVAGGGAPEIEIAKRLKDYADSISGREQLAVSAFAEALEI 439

Query: 365 IVKVLCDNAGENATERIAELKAHHREGKTTFGIISTSEGRKVDDAVEHQILDTYTAKYWG 544
           + K L +NAG ++ + + +L+A H E  +T+  I   +G K+ D  E  +++ +  K   
Sbjct: 440 VPKTLAENAGLDSIDVLVDLRAAHEE--STYMGIDVFDG-KIVDMKEAGVIEPHRVKKQA 496

Query: 545 IRFAADTAVTILRVDNII 598
           I+ AA+ A  ILR+D++I
Sbjct: 497 IQSAAEAAEMILRIDDVI 514
>sp|Q9HN70|THSA_HALSA Thermosome alpha subunit (Thermosome subunit 1) (Chaperonin alpha
           subunit)
          Length = 562

 Score =  107 bits (267), Expect = 3e-23
 Identities = 64/198 (32%), Positives = 107/198 (54%)
 Frame = +2

Query: 5   TGATPIPKLSTPTQEELGYIASVRTEEIGKSTVVLFDQDVKEGAISSIVVRGSMYDMMDE 184
           TGATP+  ++     +LG   SV  ++IG    + F +DV+E    ++++RG    ++DE
Sbjct: 325 TGATPVSNVNDIEAADLGAAGSVAQKDIGGDERI-FVEDVEEAKSVTLILRGGTEHVVDE 383

Query: 185 VERAIDDGVNTFKCLTKEPRYLAGAGATEIELANRLSDFANTQTGLVQYAIKQFVLAFES 364
           VERAI+D +   +   ++ + + G GA E ELA +L DFA++  G  Q A++ F  A E 
Sbjct: 384 VERAIEDSLGVVRVTLEDGQVMPGGGAPETELAMQLRDFADSVGGREQLAVEAFADALEV 443

Query: 365 IVKVLCDNAGENATERIAELKAHHREGKTTFGIISTSEGRKVDDAVEHQILDTYTAKYWG 544
           I + L +NAG +  + + +L++ H  G T  G+   +    V D     I++    K   
Sbjct: 444 IPRTLAENAGHDPIDSLVDLRSQHDGGDTEAGL--DAYNGDVIDMESEGIVEPLRVKTQA 501

Query: 545 IRFAADTAVTILRVDNII 598
           I  A + A  ILR+D++I
Sbjct: 502 IESATEAATMILRIDDVI 519
>sp|Q9YDK6|THSA_AERPE Thermosome alpha subunit (Thermosome subunit 1) (Chaperonin alpha
           subunit)
          Length = 554

 Score =  107 bits (266), Expect = 4e-23
 Identities = 63/198 (31%), Positives = 112/198 (56%)
 Frame = +2

Query: 5   TGATPIPKLSTPTQEELGYIASVRTEEIGKSTVVLFDQDVKEGAISSIVVRGSMYDMMDE 184
           TGA  +  L     E LGY   V   ++G+  +V F +  K     +I++RG+   ++DE
Sbjct: 330 TGAKIVTSLRDLKPEYLGYAELVEERKVGEDKMV-FIEGAKNPKSVTILLRGANDMLLDE 388

Query: 185 VERAIDDGVNTFKCLTKEPRYLAGAGATEIELANRLSDFANTQTGLVQYAIKQFVLAFES 364
            ER I D ++  + + +EP+ + G GA E+ELA +L +FA T  G  Q AI+ +  A E+
Sbjct: 389 AERNIKDALHGLRNILREPKIVGGGGAVEVELALKLKEFARTVGGKQQLAIEAYAEALET 448

Query: 365 IVKVLCDNAGENATERIAELKAHHREGKTTFGIISTSEGRKVDDAVEHQILDTYTAKYWG 544
           I  VL ++AG +A E + +L++ H +G   F  ++  EG+  +D  +  + +    K   
Sbjct: 449 IPTVLAESAGMDALEALLKLRSLHSQG-YKFAGVNVLEGKIEEDMTKINVYEPVLVKKQV 507

Query: 545 IRFAADTAVTILRVDNII 598
           I+ A++ A++IL++D++I
Sbjct: 508 IKSASEAAISILKIDDVI 525
>sp|O93624|THS_METTL Thermosome subunit (Chaperonin subunit)
          Length = 544

 Score =  105 bits (262), Expect = 1e-22
 Identities = 58/198 (29%), Positives = 107/198 (54%)
 Frame = +2

Query: 5   TGATPIPKLSTPTQEELGYIASVRTEEIGKSTVVLFDQDVKEGAISSIVVRGSMYDMMDE 184
           TGA  +  +     E+LG    V   +I    ++ F ++ K     ++++RG+   +++E
Sbjct: 323 TGANVVTNIKDLKAEDLGEAGIVEERKIAGDAMI-FVEECKHPKAVTMLIRGTTEHVIEE 381

Query: 185 VERAIDDGVNTFKCLTKEPRYLAGAGATEIELANRLSDFANTQTGLVQYAIKQFVLAFES 364
           V RA+DD +    C  ++ + +AG GA EIELA +L D+A   +G  Q A++ F  A E 
Sbjct: 382 VARAVDDAIGVVACTIEDGKIVAGGGAAEIELAMKLRDYAEGVSGREQLAVRAFADALEV 441

Query: 365 IVKVLCDNAGENATERIAELKAHHREGKTTFGIISTSEGRKVDDAVEHQILDTYTAKYWG 544
           + + L +NAG +A E + +L+A H EG   +  ++   G  V++  E+ +++    K   
Sbjct: 442 VPRTLAENAGLDAIEMLVKLRAKHAEGNNAYYGLNVFTG-DVENMTENGVVEPLRVKTQA 500

Query: 545 IRFAADTAVTILRVDNII 598
           I+ A +    +LR+D++I
Sbjct: 501 IQSATEATEMLLRIDDVI 518
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 85,428,010
Number of Sequences: 369166
Number of extensions: 1696247
Number of successful extensions: 5072
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4732
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5030
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6582889600
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)