Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_021_E06
(894 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9V0D5|MDH_PYRAB Malate dehydrogenase 213 8e-55
sp|O59028|MDH_PYRHO Malate dehydrogenase 208 2e-53
sp|P16142|MDH_METFE Malate/L-sulfolactate dehydrogenase ((R... 181 3e-45
sp|Q58820|COMC_METJA L-sulfolactate dehydrogenase ((R)-sulf... 174 3e-43
sp|O27273|COMC_METTH L-sulfolactate dehydrogenase ((R)-sulf... 163 5e-40
sp|O34736|YJMC_BACSU Hypothetical oxidoreductase yjmC 160 4e-39
sp|P58408|ALLD_ECO57 Ureidoglycolate dehydrogenase 140 6e-33
sp|P77555|ALLD_ECOLI Ureidoglycolate dehydrogenase 138 2e-32
sp|P58409|YBIC_ECO57 Hypothetical oxidoreductase ybiC 119 1e-26
sp|P30178|YBIC_ECOLI Hypothetical oxidoreductase ybiC 117 3e-26
>sp|Q9V0D5|MDH_PYRAB Malate dehydrogenase
Length = 362
Score = 213 bits (541), Expect = 8e-55
Identities = 119/270 (44%), Positives = 171/270 (63%), Gaps = 3/270 (1%)
Frame = +2
Query: 80 FKKCMSTESVIIAPT-EVREFCIKSMNSVQTKHEHSKALADVLLAADYSGHYSHGLNRLE 256
F+K E+ + P E+ F ++ + + T E +K +AD L+ AD G SHG+ RL+
Sbjct: 2 FEKGYVDENYVRIPKDELFSFVVRVLTKLGTPEEDAKIVADNLIMADLRGIESHGVQRLK 61
Query: 257 MYVDDIKANITSKSNEPCIISEKGATAFVDGMNCLGPVVGNFSMDIAINKALQVGVGWVA 436
YVD I + + II E + A +DG G VVG +M +AI KA + G+G VA
Sbjct: 62 RYVDGILSGGINLHPRIKIIREGPSYALLDGDEGFGQVVGYKAMKLAIEKARKTGIGIVA 121
Query: 437 AKGSNHYGIAGWYSQRACDKGLLGMSFTNTSPLAFPTRSKQVAVGTNPLALAAPGEKESD 616
+ SNHYGIAG+Y+ A ++G++G+S TN+ PL PT + +GTNP+ALAAP +
Sbjct: 122 VRNSNHYGIAGYYALMAAEEGMIGISMTNSRPLVAPTGGVERILGTNPIALAAP--TKGK 179
Query: 617 NFLLDMATTAVALGKIEMARRKKEPIPEGWAADANGKVTKIADEGAKGG-LLPLGGI-EI 790
FLLDMAT+ V +GK+E+ RRK E IPEGWA ++ G++T+ +E GG LLPLGG E+
Sbjct: 180 PFLLDMATSVVPIGKLEVYRRKGEEIPEGWAINSKGEITRSVEEVFNGGSLLPLGGFGEL 239
Query: 791 TGGYKGYGLSMLVEIFCGILSGSLYGPQIR 880
GG+KGYGLS++V+I GILSG + ++
Sbjct: 240 LGGHKGYGLSLMVDILSGILSGGTWSKHVK 269
>sp|O59028|MDH_PYRHO Malate dehydrogenase
Length = 360
Score = 208 bits (529), Expect = 2e-53
Identities = 118/270 (43%), Positives = 169/270 (62%), Gaps = 3/270 (1%)
Frame = +2
Query: 80 FKKCMSTESVIIAPTE-VREFCIKSMNSVQTKHEHSKALADVLLAADYSGHYSHGLNRLE 256
F+K E+ I P + + F ++ + + E +K +AD L+ AD G SHG+ RL+
Sbjct: 2 FEKGYVDENYIRVPKDRLFSFIVRVLTKLGVPEEDAKIVADNLVMADLRGVESHGVQRLK 61
Query: 257 MYVDDIKANITSKSNEPCIISEKGATAFVDGMNCLGPVVGNFSMDIAINKALQVGVGWVA 436
YVD I + + + +I E + A +DG LG VVG SM +AI KA G+G V
Sbjct: 62 RYVDGIISGGVNLHPKIRVIREGPSYALIDGDEGLGQVVGYRSMKLAIKKAKDTGIGIVI 121
Query: 437 AKGSNHYGIAGWYSQRACDKGLLGMSFTNTSPLAFPTRSKQVAVGTNPLALAAPGEKESD 616
A+ SNHYGIAG+Y+ A ++G++G+S TN+ PL PT + +GTNP+ALAAP + +
Sbjct: 122 ARNSNHYGIAGYYALMAAEEGMIGISMTNSRPLVAPTGGIERILGTNPIALAAPTKDKP- 180
Query: 617 NFLLDMATTAVALGKIEMARRKKEPIPEGWAADANGKVTKIADEGAKGG-LLPLGGI-EI 790
FLLDMAT+ V +GK+E+ RRK + IPEGWA + G +T +E GG LLPLGG E+
Sbjct: 181 -FLLDMATSVVPIGKLEVYRRKGKDIPEGWAINREGNITTKVEEVFNGGALLPLGGFGEL 239
Query: 791 TGGYKGYGLSMLVEIFCGILSGSLYGPQIR 880
GG+KGYGLS++V+I GILSG + ++
Sbjct: 240 LGGHKGYGLSLMVDILSGILSGGTWSKYVK 269
>sp|P16142|MDH_METFE Malate/L-sulfolactate dehydrogenase ((R)-2-hydroxyacid
dehydrogenase)
Length = 339
Score = 181 bits (458), Expect = 3e-45
Identities = 102/258 (39%), Positives = 141/258 (54%)
Frame = +2
Query: 107 VIIAPTEVREFCIKSMNSVQTKHEHSKALADVLLAADYSGHYSHGLNRLEMYVDDIKANI 286
+II+P E R IK +N++ EH+K ADV++ AD G SHG+ R YV+ IK
Sbjct: 1 MIISPEEERSLIIKILNALGVSEEHAKITADVIVDADLKGFTSHGIGRFPQYVEGIKLGT 60
Query: 287 TSKSNEPCIISEKGATAFVDGMNCLGPVVGNFSMDIAINKALQVGVGWVAAKGSNHYGIA 466
S I E + A ++G + LG VV M +AI KA GVG V SNH+GIA
Sbjct: 61 IKTSGNIEIEKETDSVALINGNHLLGQVVAYKGMKLAIEKAKNTGVGIVGIHDSNHFGIA 120
Query: 467 GWYSQRACDKGLLGMSFTNTSPLAFPTRSKQVAVGTNPLALAAPGEKESDNFLLDMATTA 646
G+YS A ++G++ TNT P P K +GTNP+A++ P + +DM+T A
Sbjct: 121 GYYSDMAMKNDMIGITMTNTEPAVAPLGGKIPVLGTNPIAISIPSNEY--YVAVDMSTAA 178
Query: 647 VALGKIEMARRKKEPIPEGWAADANGKVTKIADEGAKGGLLPLGGIEITGGYKGYGLSML 826
VA GK+ A RK E IPEG A D NG T +E G +LP GG+KGY L +
Sbjct: 179 VARGKLLEAARKNEKIPEGIAVDKNGNPTTDPNEALNGSILPF------GGHKGYALCFM 232
Query: 827 VEIFCGILSGSLYGPQIR 880
+EI G L + +G +++
Sbjct: 233 IEILAGPLVKAEFGSKVK 250
>sp|Q58820|COMC_METJA L-sulfolactate dehydrogenase ((R)-sulfolactate dehydrogenase)
((R)-2-hydroxyacid dehydrogenase)
Length = 344
Score = 174 bits (441), Expect = 3e-43
Identities = 101/258 (39%), Positives = 147/258 (56%)
Frame = +2
Query: 107 VIIAPTEVREFCIKSMNSVQTKHEHSKALADVLLAADYSGHYSHGLNRLEMYVDDIKANI 286
+I+ P ++ I + E +K ADV + AD G SHG+ R Y+ +K
Sbjct: 1 MILKPENEKKLIIDVLKKFGVPEEDAKITADVFVDADLKGFTSHGIGRFPQYITALKLGN 60
Query: 287 TSKSNEPCIISEKGATAFVDGMNCLGPVVGNFSMDIAINKALQVGVGWVAAKGSNHYGIA 466
+ + I+ E ATA +DG LG VVG +M++AI KA VGVG VA + +NH+GIA
Sbjct: 61 INPKPDIKIVKESPATAVIDGDLGLGQVVGKKAMELAIKKAKNVGVGVVATRNANHFGIA 120
Query: 467 GWYSQRACDKGLLGMSFTNTSPLAFPTRSKQVAVGTNPLALAAPGEKESDNFLLDMATTA 646
G+YS+ A ++ ++G++ TNT P P K+ +GTNP+A+A G K F LDMAT +
Sbjct: 121 GYYSELAMNQDMIGITITNTEPAMAPFGGKEKILGTNPIAIAFKGNKY--KFSLDMATAS 178
Query: 647 VALGKIEMARRKKEPIPEGWAADANGKVTKIADEGAKGGLLPLGGIEITGGYKGYGLSML 826
+A GKI A RKK IPEG A D +GK T + +G +LP GG KGYGL++
Sbjct: 179 IARGKILEALRKKIKIPEGCAVDKDGKPTTDPAKALEGCILPF------GGPKGYGLALA 232
Query: 827 VEIFCGILSGSLYGPQIR 880
+E+ I G+ G +++
Sbjct: 233 IEMLSAI-GGAEVGTKVK 249
>sp|O27273|COMC_METTH L-sulfolactate dehydrogenase ((R)-sulfolactate dehydrogenase)
Length = 341
Score = 163 bits (413), Expect = 5e-40
Identities = 94/255 (36%), Positives = 136/255 (53%)
Frame = +2
Query: 113 IAPTEVREFCIKSMNSVQTKHEHSKALADVLLAADYSGHYSHGLNRLEMYVDDIKANITS 292
I+P E + + + ++ E S +ADV L AD G SHG+ R YVD ++
Sbjct: 3 ISPEEEVKIIKEILTAMNVPEESSDIVADVTLDADLKGFSSHGIGRFPQYVDGLRHGTIR 62
Query: 293 KSNEPCIISEKGATAFVDGMNCLGPVVGNFSMDIAINKALQVGVGWVAAKGSNHYGIAGW 472
+ I E +TA ++G + G VV +M++AI KA GVG V SNH+G+AG+
Sbjct: 63 ADGDITIERETESTALINGNHIFGHVVAYRAMELAIEKARNTGVGLVGVHDSNHFGVAGY 122
Query: 473 YSQRACDKGLLGMSFTNTSPLAFPTRSKQVAVGTNPLALAAPGEKESDNFLLDMATTAVA 652
YS A ++G+ NT P P ++ +GTNP+A+ P + + +DMAT+A A
Sbjct: 123 YSDMAVMNDMIGVVIANTEPAVAPIGGRKPILGTNPVAIGIPSNRYYVS--VDMATSASA 180
Query: 653 LGKIEMARRKKEPIPEGWAADANGKVTKIADEGAKGGLLPLGGIEITGGYKGYGLSMLVE 832
GK+ A RK E IPE A DA GK T + KG +LP GG+KGY LS ++E
Sbjct: 181 RGKLLEAARKGESIPENVALDAEGKPTTDPEMALKGSILPF------GGHKGYALSFMIE 234
Query: 833 IFCGILSGSLYGPQI 877
I G L G+ +G +
Sbjct: 235 ILAGPLVGAAFGTAV 249
>sp|O34736|YJMC_BACSU Hypothetical oxidoreductase yjmC
Length = 337
Score = 160 bits (405), Expect = 4e-39
Identities = 91/261 (34%), Positives = 142/261 (54%)
Frame = +2
Query: 101 ESVIIAPTEVREFCIKSMNSVQTKHEHSKALADVLLAADYSGHYSHGLNRLEMYVDDIKA 280
+++ IA E +E + ++ ++ +ADVL+ AD +SHG+ E YV+ + A
Sbjct: 2 KTITIAAEEAKELVWQKLDGAGLNERDAEKVADVLVHADLRNVHSHGVLHTEHYVNRLLA 61
Query: 281 NITSKSNEPCIISEKGATAFVDGMNCLGPVVGNFSMDIAINKALQVGVGWVAAKGSNHYG 460
+ +P T +DG + G V + +MD AI+ A + GVG V A S+H G
Sbjct: 62 GGINPGAQPVFKETGPVTGVLDGDDGFGHVNCDMAMDHAIDMAKKKGVGMVTAVNSSHCG 121
Query: 461 IAGWYSQRACDKGLLGMSFTNTSPLAFPTRSKQVAVGTNPLALAAPGEKESDNFLLDMAT 640
++ Q+A D+ L+GM+ T+T + P + +GTNP+A P K F+LDMAT
Sbjct: 122 ALSYFVQKAADEKLIGMAMTHTDSIVVPFGGRTPILGTNPIAYGVPA-KHKKPFILDMAT 180
Query: 641 TAVALGKIEMARRKKEPIPEGWAADANGKVTKIADEGAKGGLLPLGGIEITGGYKGYGLS 820
+ VA GKI AR + + IPEGW D NG+ D+ + + GG KGYGLS
Sbjct: 181 SKVAFGKILQAREEGKEIPEGWGVDENGEAVTDPDK--------VVSLSTFGGPKGYGLS 232
Query: 821 MLVEIFCGILSGSLYGPQIRK 883
++V++F G+L+G+ +GP I K
Sbjct: 233 IVVDVFSGLLAGAAFGPHIAK 253
>sp|P58408|ALLD_ECO57 Ureidoglycolate dehydrogenase
Length = 349
Score = 140 bits (352), Expect = 6e-33
Identities = 86/236 (36%), Positives = 120/236 (50%)
Frame = +2
Query: 170 KHEHSKALADVLLAADYSGHYSHGLNRLEMYVDDIKANITSKSNEPCIISEKGATAFVDG 349
K EH+ +A+VL+ AD G +SHG R+E Y + I T++ E + +A +
Sbjct: 22 KREHAATVAEVLVYADARGIHSHGAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHA 81
Query: 350 MNCLGPVVGNFSMDIAINKALQVGVGWVAAKGSNHYGIAGWYSQRACDKGLLGMSFTNTS 529
N G V M+ AI A Q GV V H G ++ Q+A GL+G+S +
Sbjct: 82 DNAAGQVAAKMGMEHAIKTAQQNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSD 141
Query: 530 PLAFPTRSKQVAVGTNPLALAAPGEKESDNFLLDMATTAVALGKIEMARRKKEPIPEGWA 709
P+ P ++ GTNPLA AAPGE + + DMATT A GK+ AR + IP+ WA
Sbjct: 142 PMVVPFGGAEIYYGTNPLAFAAPGEGD-EILTFDMATTVQAWGKVLDARSRNMSIPDTWA 200
Query: 710 ADANGKVTKIADEGAKGGLLPLGGIEITGGYKGYGLSMLVEIFCGILSGSLYGPQI 877
D NG T D A LLP G KGYGL M++++ G+L G +G Q+
Sbjct: 201 VDKNGAPT--TDPFAVHALLP------AAGPKGYGLMMMIDVLSGVLLGLPFGRQV 248
>sp|P77555|ALLD_ECOLI Ureidoglycolate dehydrogenase
Length = 349
Score = 138 bits (347), Expect = 2e-32
Identities = 85/236 (36%), Positives = 119/236 (50%)
Frame = +2
Query: 170 KHEHSKALADVLLAADYSGHYSHGLNRLEMYVDDIKANITSKSNEPCIISEKGATAFVDG 349
K EH+ +A+VL+ AD G +SHG R+E Y + I T++ E + +A +
Sbjct: 22 KREHAATVAEVLVYADARGIHSHGAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHA 81
Query: 350 MNCLGPVVGNFSMDIAINKALQVGVGWVAAKGSNHYGIAGWYSQRACDKGLLGMSFTNTS 529
N G V M+ AI A Q GV V H G ++ Q+A G +G+S +
Sbjct: 82 DNAAGQVAAKMGMEHAIKTAQQNGVAVVGISRMGHSGAISYFVQQAARAGFIGISMCQSD 141
Query: 530 PLAFPTRSKQVAVGTNPLALAAPGEKESDNFLLDMATTAVALGKIEMARRKKEPIPEGWA 709
P+ P ++ GTNPLA AAPGE + + DMATT A GK+ AR + IP+ WA
Sbjct: 142 PMVVPFGGAEIYYGTNPLAFAAPGEGD-EILTFDMATTVQAWGKVLDARSRNMSIPDTWA 200
Query: 710 ADANGKVTKIADEGAKGGLLPLGGIEITGGYKGYGLSMLVEIFCGILSGSLYGPQI 877
D NG T D A LLP G KGYGL M++++ G+L G +G Q+
Sbjct: 201 VDKNGVPT--TDPFAVHALLP------AAGPKGYGLMMMIDVLSGVLLGLPFGRQV 248
>sp|P58409|YBIC_ECO57 Hypothetical oxidoreductase ybiC
Length = 361
Score = 119 bits (298), Expect = 1e-26
Identities = 75/232 (32%), Positives = 114/232 (49%), Gaps = 2/232 (0%)
Frame = +2
Query: 167 TKHEHSKALADVLLAADYSGHYSHGLNRLEMYVDDIKANITSKSNEPCIISEKGATAFVD 346
++ + +K +AD L+AA+ +GH SHG+ + YV ++ I+ E GA +D
Sbjct: 25 SEEQEAKLVADHLIAANLAGHDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLD 84
Query: 347 GMNCLGPVVGNFSMDIAINKALQVGVGWVAAKGSNHYGIAGWYSQRACDKGLLGMSFTNT 526
G G V + +M + I KA Q G+ VA S+H G G+++++ G + + F +
Sbjct: 85 GDRAFGQVAAHEAMALGIEKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSV 144
Query: 527 --SPLAFPTRSKQVAVGTNPLALAAPGEKESDNFLLDMATTAVALGKIEMARRKKEPIPE 700
P+ P + GTNP + P K+ LLD AT+A+A GK +A K P+P
Sbjct: 145 VGIPMVAPFHGRDSRFGTNPFCVVFP-RKDDFPLLLDYATSAIAFGKTRVAWHKGVPVPP 203
Query: 701 GWAADANGKVTKIADEGAKGGLLPLGGIEITGGYKGYGLSMLVEIFCGILSG 856
G D NG T A PLG + +KGY L+ + EI G LSG
Sbjct: 204 GCLIDVNGVPT---TNPAVMQESPLGSLLTFAEHKGYALAAMCEILGGALSG 252
>sp|P30178|YBIC_ECOLI Hypothetical oxidoreductase ybiC
Length = 361
Score = 117 bits (294), Expect = 3e-26
Identities = 74/232 (31%), Positives = 114/232 (49%), Gaps = 2/232 (0%)
Frame = +2
Query: 167 TKHEHSKALADVLLAADYSGHYSHGLNRLEMYVDDIKANITSKSNEPCIISEKGATAFVD 346
++ + +K +AD L+AA+ +GH SHG+ + YV ++ + E GA +D
Sbjct: 25 SEEQEAKLVADHLIAANLAGHDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLD 84
Query: 347 GMNCLGPVVGNFSMDIAINKALQVGVGWVAAKGSNHYGIAGWYSQRACDKGLLGMSFTNT 526
G G V + +M + I KA Q G+ VA S+H G G+++++ G + + F +
Sbjct: 85 GDRAFGQVAAHEAMALGIEKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSV 144
Query: 527 --SPLAFPTRSKQVAVGTNPLALAAPGEKESDNFLLDMATTAVALGKIEMARRKKEPIPE 700
P+ P + GTNP + P K++ LLD AT+A+A GK +A K P+P
Sbjct: 145 VGIPMVAPFHGRDSRFGTNPFCVVFP-RKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPP 203
Query: 701 GWAADANGKVTKIADEGAKGGLLPLGGIEITGGYKGYGLSMLVEIFCGILSG 856
G D NG T A PLG + +KGY L+ + EI G LSG
Sbjct: 204 GCLIDVNGVPT---TNPAVMQESPLGSLLTFAEHKGYALAAMCEILGGALSG 252
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 106,226,855
Number of Sequences: 369166
Number of extensions: 2244924
Number of successful extensions: 6430
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6039
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6390
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8982382310
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)