Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_021_D14
(804 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P42356|PI4KA_HUMAN Phosphatidylinositol 4-kinase alpha (... 295 8e-80
sp|P37297|STT4_YEAST Phosphatidylinositol 4-kinase STT4 (PI... 157 3e-38
sp|P42337|PK3CA_MOUSE Phosphatidylinositol-4,5-bisphosphate... 50 9e-06
sp|P32871|PK3CA_BOVIN Phosphatidylinositol-4,5-bisphosphate... 49 1e-05
sp|P42336|PK3CA_HUMAN Phosphatidylinositol-4,5-bisphosphate... 49 1e-05
sp|O00329|PK3CD_HUMAN Phosphatidylinositol-4,5-bisphosphate... 37 0.048
sp|P50520|VPS34_SCHPO Phosphatidylinositol 3-kinase vps34 (... 37 0.082
sp|Q8BTI9|PK3CB_MOUSE Phosphatidylinositol-4,5-bisphosphate... 36 0.14
sp|Q9Z1L0|PK3CB_RAT Phosphatidylinositol-4,5-bisphosphate 3... 36 0.14
sp|P42338|PK3CB_HUMAN Phosphatidylinositol-4,5-bisphosphate... 35 0.31
>sp|P42356|PI4KA_HUMAN Phosphatidylinositol 4-kinase alpha (PI4-kinase) (PtdIns-4-kinase)
(PI4K-alpha)
Length = 2044
Score = 295 bits (756), Expect = 8e-80
Identities = 138/228 (60%), Positives = 175/228 (76%)
Frame = +2
Query: 20 DIPELCYVPTWXXXXXXXXLSFFSRMYPQHPLTHQYAVKVLSLYPPDTLIFYVPQLVQGI 199
D PEL +V W LS+FS MYP HPLT QY VKVL +PPD ++FY+PQ+VQ +
Sbjct: 1544 DAPELSHVLCWAPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQAL 1603
Query: 200 RYDKMGYLTEFILEIVHLSPILAHQLIWNMKTNMYKDEDGQVKDPDIGLQLESVIDEINK 379
RYDKMGY+ E+IL S +LAHQ IWNMKTN+Y DE+G KDPDIG L+ +++EI
Sbjct: 1604 RYDKMGYVREYILWAASKSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGDLLDQLVEEITG 1663
Query: 380 SFSGMSLSFSEREFDFFDKITNVSGVIRPYPKGQQRQQACLEALRKIELQPGCYLPSNPD 559
S SG + F +REFDFF+KITNVS +I+PYPKG +R++ACL AL ++++QPGCYLPSNP+
Sbjct: 1664 SLSGPAKDFYQREFDFFNKITNVSAIIKPYPKGDERKKACLSALSEVKVQPGCYLPSNPE 1723
Query: 560 AIVLEIDYLSGIPLQSAAKAPYLAEFRVKRCGVQKLE*EAIRAASFSD 703
AIVL+IDY SG P+QSAAKAPYLA+F+VKRCGV +LE E +R S S+
Sbjct: 1724 AIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLRCRSDSE 1771
>sp|P37297|STT4_YEAST Phosphatidylinositol 4-kinase STT4 (PI4-kinase) (PtdIns-4-kinase)
Length = 1900
Score = 157 bits (397), Expect = 3e-38
Identities = 78/202 (38%), Positives = 124/202 (61%), Gaps = 2/202 (0%)
Frame = +2
Query: 50 WXXXXXXXXLSFFSRMYPQHPLTHQYAVKVLSLYPPDTLIFYVPQLVQGIRYDKMGYLTE 229
W ++ F + + Q+++ L + FYVPQ+VQ +RYDK GY+
Sbjct: 1425 WAPVSPLKSINLFLPEWQGNSFILQFSIYSLESQDVNLAFFYVPQIVQCLRYDKTGYVER 1484
Query: 230 FILEIVHLSPILAHQLIWNMKTNMYKDEDGQVKDPDIGLQLESVIDEINKSFSGMSLSFS 409
IL+ +S + +HQ+IWNM N YKD++G +++ +I L+ + + + SFS F
Sbjct: 1485 LILDTAKISVLFSHQIIWNMLANCYKDDEG-IQEDEIKPTLDRIRERMVSSFSQSHRDFY 1543
Query: 410 EREFDFFDKITNVSGVIRPYPKGQ--QRQQACLEALRKIELQPGCYLPSNPDAIVLEIDY 583
EREF+FFD++T +SG ++PY K +++ E + KIE++P YLPSNPD +V++ID
Sbjct: 1544 EREFEFFDEVTGISGKLKPYIKKSKAEKKHKIDEEMSKIEVKPDVYLPSNPDGVVIDIDR 1603
Query: 584 LSGIPLQSAAKAPYLAEFRVKR 649
SG PLQS AKAP++A F++K+
Sbjct: 1604 KSGKPLQSHAKAPFMATFKIKK 1625
>sp|P42337|PK3CA_MOUSE Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit,
alpha isoform (PI3-kinase p110 subunit alpha)
(PtdIns-3-kinase p110) (PI3K)
Length = 1068
Score = 49.7 bits (117), Expect = 9e-06
Identities = 51/205 (24%), Positives = 88/205 (42%), Gaps = 9/205 (4%)
Frame = +2
Query: 35 CYVPTWXXXXXXXXLSFFSRMYPQHPLTHQYAVKVLSLY-PPDTLIFYVPQLVQGIRYDK 211
C V W + YP P+ +AV+ L Y D L Y+ QLVQ ++Y++
Sbjct: 585 CLVKDWPPIKPEQAMELLDCNYPD-PMVRSFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQ 643
Query: 212 M--GYLTEFILEIVHLSPILAHQLIWNMKTNMYKDEDGQVKDPDIGLQLESVIDEINKSF 385
L F+L+ + + H W++K+ M+ Q GL LES
Sbjct: 644 YLDNLLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQ----RFGLLLESYC-----RA 694
Query: 386 SGMSLSFSEREFDFFDKITNVSGVIRPYPKGQQRQ---QACLEALRK---IELQPGCYLP 547
GM L R+ + +K+ N++ +++ K + ++ + +E +R+ ++ G P
Sbjct: 695 CGMYLKHLNRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRQPDFMDALQGFLSP 754
Query: 548 SNPDAIVLEIDYLSGIPLQSAAKAP 622
NP A L L + S+AK P
Sbjct: 755 LNP-AHQLGNLRLEECRIMSSAKRP 778
>sp|P32871|PK3CA_BOVIN Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit,
alpha isoform (PI3-kinase p110 subunit alpha)
(PtdIns-3-kinase p110) (PI3K)
Length = 1068
Score = 49.3 bits (116), Expect = 1e-05
Identities = 51/205 (24%), Positives = 88/205 (42%), Gaps = 9/205 (4%)
Frame = +2
Query: 35 CYVPTWXXXXXXXXLSFFSRMYPQHPLTHQYAVKVLSLY-PPDTLIFYVPQLVQGIRYDK 211
C V W + YP P+ +AV+ L Y D L Y+ QLVQ ++Y++
Sbjct: 585 CLVKDWPPIKPEQAMELLDCNYPD-PMVRGFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQ 643
Query: 212 M--GYLTEFILEIVHLSPILAHQLIWNMKTNMYKDEDGQVKDPDIGLQLESVIDEINKSF 385
L F+L+ + + H W++K+ M+ Q GL LES
Sbjct: 644 YLDNLLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQ----RFGLLLESYC-----RA 694
Query: 386 SGMSLSFSEREFDFFDKITNVSGVIRPYPKGQQRQ---QACLEALRK---IELQPGCYLP 547
GM L R+ + +K+ N++ +++ K + ++ + +E +R+ ++ G P
Sbjct: 695 CGMYLKHLNRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRRPDFMDALQGFLSP 754
Query: 548 SNPDAIVLEIDYLSGIPLQSAAKAP 622
NP A L L + S+AK P
Sbjct: 755 LNP-AHQLGNLRLEECRIMSSAKRP 778
>sp|P42336|PK3CA_HUMAN Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit,
alpha isoform (PI3-kinase p110 subunit alpha)
(PtdIns-3-kinase p110) (PI3K)
Length = 1068
Score = 49.3 bits (116), Expect = 1e-05
Identities = 51/205 (24%), Positives = 88/205 (42%), Gaps = 9/205 (4%)
Frame = +2
Query: 35 CYVPTWXXXXXXXXLSFFSRMYPQHPLTHQYAVKVLSLY-PPDTLIFYVPQLVQGIRYDK 211
C V W + YP P+ +AV+ L Y D L Y+ QLVQ ++Y++
Sbjct: 585 CLVKDWPPIKPEQAMELLDCNYPD-PMVRGFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQ 643
Query: 212 M--GYLTEFILEIVHLSPILAHQLIWNMKTNMYKDEDGQVKDPDIGLQLESVIDEINKSF 385
L F+L+ + + H W++K+ M+ Q GL LES
Sbjct: 644 YLDNLLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQ----RFGLLLESYC-----RA 694
Query: 386 SGMSLSFSEREFDFFDKITNVSGVIRPYPKGQQRQ---QACLEALRK---IELQPGCYLP 547
GM L R+ + +K+ N++ +++ K + ++ + +E +R+ ++ G P
Sbjct: 695 CGMYLKHLNRQVEAMEKLINLTDILKQERKDETQKVQMKFLVEQMRRPDFMDALQGLLSP 754
Query: 548 SNPDAIVLEIDYLSGIPLQSAAKAP 622
NP A L L + S+AK P
Sbjct: 755 LNP-AHQLGNLRLKECRIMSSAKRP 778
>sp|O00329|PK3CD_HUMAN Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit,
delta isoform (PI3-kinase p110 subunit delta)
(PtdIns-3-kinase p110) (PI3K) (p110delta)
Length = 1044
Score = 37.4 bits (85), Expect = 0.048
Identities = 32/158 (20%), Positives = 67/158 (42%), Gaps = 7/158 (4%)
Frame = +2
Query: 125 YAVKVLSLYPPDTLIFYVPQLVQGIRYDKM--GYLTEFILEIVHLSPILAHQLIWNMKTN 298
+A+K L D L Y+ QLVQ ++Y+ LT+F+L+ + + H L W++++
Sbjct: 595 FAIKSLRKLTDDELFQYLLQLVQVLKYESYLDCELTKFLLDRALANRKIGHFLFWHLRSE 654
Query: 299 MYKDEDGQVKDPDIGLQLESVIDEINK-SFSGMSLSFSERE----FDFFDKITNVSGVIR 463
M+ P + L+ +++ + S M + + E + +S
Sbjct: 655 MHV--------PSVALRFGLILEAYCRGSTHHMKVLMKQGEALSKLKALNDFVKLSSQKT 706
Query: 464 PYPKGQQRQQACLEALRKIELQPGCYLPSNPDAIVLEI 577
P P+ ++ C+ +E P +P ++ E+
Sbjct: 707 PKPQTKELMHLCMRQEAYLEALSHLQSPLDPSTLLAEV 744
>sp|P50520|VPS34_SCHPO Phosphatidylinositol 3-kinase vps34 (PI3-kinase) (PtdIns-3-kinase)
(PI3K) (Vacuolar sorting protein 34)
Length = 801
Score = 36.6 bits (83), Expect = 0.082
Identities = 31/135 (22%), Positives = 57/135 (42%), Gaps = 14/135 (10%)
Frame = +2
Query: 107 HPLTHQYAVKVLSLYPPDTLIFYVPQLVQGIRYD---------KMGYLTEFILEIVHLSP 259
HP YAV L + L+ Y+ QLVQ +RYD + L F++ SP
Sbjct: 347 HPKVRAYAVSRLETASNEELLLYLLQLVQALRYDNPISSDERFQPSPLALFLVNRAISSP 406
Query: 260 ILAHQLIWNMKTNMYKDEDGQVKDPDIGLQLESVI----DEINKSFSGMSLSFS-EREFD 424
+ + L W + +++D + SV+ E++KS G + + +
Sbjct: 407 SIGNDLYWYLVV--------EIEDEPVSKLFSSVMFLFQKELSKSVEGRLIRETLSAQAK 458
Query: 425 FFDKITNVSGVIRPY 469
F +K+ +S ++ +
Sbjct: 459 FVEKLLRISKSVQSF 473
>sp|Q8BTI9|PK3CB_MOUSE Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit,
beta isoform (PI3-kinase p110 subunit beta)
(PtdIns-3-kinase p110) (PI3K) (PI3Kbeta)
Length = 1064
Score = 35.8 bits (81), Expect = 0.14
Identities = 37/184 (20%), Positives = 73/184 (39%), Gaps = 8/184 (4%)
Frame = +2
Query: 50 WXXXXXXXXLSFFSRMYPQHPLTHQYAVKVLSLYPPDTLIFYVPQLVQGIRYDKM--GYL 223
W L YP +YAV L + L Y+ QLVQ ++Y+ L
Sbjct: 592 WPKLPpreALELLDFNYPDQ-YVREYAVGCLRQMSDEELSQYLLQLVQVLKYEPFLDCAL 650
Query: 224 TEFILEIVHLSPILAHQLIWNMKTNMYKDEDGQVKDPDIGLQLESVIDEINKSFSGMSLS 403
+ F+LE + + L W++++ +V P + +Q +++ + G +
Sbjct: 651 SRFLLERALDNRRIGQFLFWHLRS--------EVHTPAVSVQFGVILEAYCRGSVG-HMK 701
Query: 404 FSEREFDFFDKITNVSGVIR------PYPKGQQRQQACLEALRKIELQPGCYLPSNPDAI 565
++ + +K+ ++ +I+ KG++ CL+ E P NP I
Sbjct: 702 VLSKQVEALNKLKTLNSLIKLNAVKLSRAKGKEAMHTCLKQSAYREALSDLQSPLNPCVI 761
Query: 566 VLEI 577
+ E+
Sbjct: 762 LSEL 765
>sp|Q9Z1L0|PK3CB_RAT Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit,
beta isoform (PI3-kinase p110 subunit beta)
(PtdIns-3-kinase p110) (PI3K) (PI3Kbeta)
Length = 1070
Score = 35.8 bits (81), Expect = 0.14
Identities = 37/184 (20%), Positives = 73/184 (39%), Gaps = 8/184 (4%)
Frame = +2
Query: 50 WXXXXXXXXLSFFSRMYPQHPLTHQYAVKVLSLYPPDTLIFYVPQLVQGIRYDKM--GYL 223
W L YP +YAV L + L Y+ QLVQ ++Y+ L
Sbjct: 598 WPKLPpreALELLDFNYPDQ-YVREYAVGCLRQMSDEELSQYLLQLVQVLKYEPFLDCAL 656
Query: 224 TEFILEIVHLSPILAHQLIWNMKTNMYKDEDGQVKDPDIGLQLESVIDEINKSFSGMSLS 403
+ F+LE + + L W++++ +V P + +Q +++ + G +
Sbjct: 657 SRFLLERALDNRRIGQFLFWHLRS--------EVHTPAVSIQFGVILEAYCRGSVG-HMK 707
Query: 404 FSEREFDFFDKITNVSGVIR------PYPKGQQRQQACLEALRKIELQPGCYLPSNPDAI 565
++ + +K+ ++ +I+ KG++ CL+ E P NP I
Sbjct: 708 VLSKQVEALNKLKTLNSLIKLNAMKLNRAKGKEAMHTCLKQSAYREALSDLQSPLNPCVI 767
Query: 566 VLEI 577
+ E+
Sbjct: 768 LSEL 771
>sp|P42338|PK3CB_HUMAN Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit,
beta isoform (PI3-kinase p110 subunit beta)
(PtdIns-3-kinase p110) (PI3K) (PI3Kbeta)
Length = 1070
Score = 34.7 bits (78), Expect = 0.31
Identities = 37/184 (20%), Positives = 73/184 (39%), Gaps = 8/184 (4%)
Frame = +2
Query: 50 WXXXXXXXXLSFFSRMYPQHPLTHQYAVKVLSLYPPDTLIFYVPQLVQGIRYDKM--GYL 223
W L YP +YAV L + L Y+ QLVQ ++Y+ L
Sbjct: 598 WPKLPpreALELLDFNYPDQ-YVREYAVGCLRQMSDEELSQYLLQLVQVLKYEPFLDCAL 656
Query: 224 TEFILEIVHLSPILAHQLIWNMKTNMYKDEDGQVKDPDIGLQLESVIDEINKSFSGMSLS 403
+ F+LE + + L W++++ +V P + +Q +++ + G +
Sbjct: 657 SRFLLERALGNRRIGQFLFWHLRS--------EVHIPAVSVQFGVILEAYCRGSVG-HMK 707
Query: 404 FSEREFDFFDKITNVSGVIR------PYPKGQQRQQACLEALRKIELQPGCYLPSNPDAI 565
++ + +K+ ++ +I+ KG++ CL+ E P NP I
Sbjct: 708 VLSKQVEALNKLKTLNSLIKLNAVKLNRAKGKEAMHTCLKQSAYREALSDLQSPLNPCVI 767
Query: 566 VLEI 577
+ E+
Sbjct: 768 LSEL 771
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 89,987,720
Number of Sequences: 369166
Number of extensions: 1724150
Number of successful extensions: 3870
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3771
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3863
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7618580670
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)