Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_021_B08
(724 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9UKR3|KLK13_HUMAN Kallikrein 13 precursor (Kallikrein-l... 129 1e-29
sp|Q61955|NRPN_MOUSE Neuropsin precursor (NP) (Kallikrein 8) 113 4e-25
sp|O88780|NRPN_RAT Neuropsin precursor (NP) (Kallikrein-8) ... 113 4e-25
sp|P00765|TRYP_ASTFL Trypsin I 112 1e-24
sp|P26262|KLKB1_MOUSE Plasma kallikrein precursor (Plasma p... 110 3e-24
sp|Q91VE3|KLK7_MOUSE Kallikrein-7 precursor (Stratum corneu... 110 4e-24
sp|P47796|CTRA_GADMO Chymotrypsin A precursor 110 5e-24
sp|P15949|KLK9_MOUSE Glandular kallikrein K9 precursor (Tis... 108 1e-23
sp|Q9JHJ7|TEST_MOUSE Testisin precursor (Tryptase 4) 108 2e-23
sp|P36368|KLK13_MOUSE Glandular kallikrein K13 precursor (T... 108 2e-23
>sp|Q9UKR3|KLK13_HUMAN Kallikrein 13 precursor (Kallikrein-like protein 4) (KLK-L4)
Length = 277
Score = 129 bits (323), Expect = 1e-29
Identities = 79/219 (36%), Positives = 112/219 (51%), Gaps = 1/219 (0%)
Frame = +3
Query: 9 CGGTLLNGNTVLTAAHCVWDMRNWDVNTFKYYQMKVYMGEYDLYRMYGVEKIFQVTKTII 188
CGG L++ VLTAAHC+ + +KVY+G++ L R+ E++ +V +I
Sbjct: 61 CGGVLVHPKWVLTAAHCLKE------------GLKVYLGKHALGRVEAGEQVREVVHSIP 108
Query: 189 HPSYRHEYAYLE-GNDVALIKFDGYISKNQYDYIDYNELDVNAISKPGDRCEVVGWGSML 365
HP YR +L +D+ L++ + YI L N PG C V GWG+
Sbjct: 109 HPEYRRSPTHLNHDHDIMLLELQSPVQLT--GYIQTLPLSHNNRLTPGTTCRVSGWGTTT 166
Query: 366 PPGQDTSRFLQKAAINIISNSECGRIFSNVAYVRSNICAGSGRLSTSSCRGDSGGPLFCT 545
P + + LQ A I + S+ EC +++ + +CAG+ SC GDSGGPL C
Sbjct: 167 SPQVNYPKTLQCANIQLRSDEECRQVYPG-KITDNMLCAGTKEGGKDSCEGDSGGPLVC- 224
Query: 546 DGRKHHIQVGIVSYGTVPCGQPNVPSVYTRVSEVAGWIR 662
+ GIVS+G PCGQP+ P VYTRVS WIR
Sbjct: 225 ----NRTLYGIVSWGDFPCGQPDRPGVYTRVSRYVLWIR 259
>sp|Q61955|NRPN_MOUSE Neuropsin precursor (NP) (Kallikrein 8)
Length = 260
Score = 113 bits (283), Expect = 4e-25
Identities = 72/218 (33%), Positives = 105/218 (48%)
Frame = +3
Query: 9 CGGTLLNGNTVLTAAHCVWDMRNWDVNTFKYYQMKVYMGEYDLYRMYGVEKIFQVTKTII 188
CGG L+ VLTAAHC K + V +G++ L E+ QV ++I
Sbjct: 58 CGGVLVGDRWVLTAAHC------------KKQKYSVRLGDHSLQSRDQPEQEIQVAQSIQ 105
Query: 189 HPSYRHEYAYLEGNDVALIKFDGYISKNQYDYIDYNELDVNAISKPGDRCEVVGWGSMLP 368
HP Y + +D+ LI+ S N D + +L N K G +C + GWG++
Sbjct: 106 HPCYNNSNPEDHSHDIMLIRLQN--SANLGDKVKPVQL-ANLCPKVGQKCIISGWGTVTS 162
Query: 369 PGQDTSRFLQKAAINIISNSECGRIFSNVAYVRSNICAGSGRLSTSSCRGDSGGPLFCTD 548
P ++ L A + I S ++C R + +CAGS +C+GDSGGPL C D
Sbjct: 163 PQENFPNTLNCAEVKIYSQNKCERAYPG-KITEGMVCAGSSN-GADTCQGDSGGPLVC-D 219
Query: 549 GRKHHIQVGIVSYGTVPCGQPNVPSVYTRVSEVAGWIR 662
G GI S+G+ PCG+P P VYT++ WI+
Sbjct: 220 GMLQ----GITSWGSDPCGKPEKPGVYTKICRYTTWIK 253
>sp|O88780|NRPN_RAT Neuropsin precursor (NP) (Kallikrein-8) (Brain serine protease 1)
Length = 260
Score = 113 bits (283), Expect = 4e-25
Identities = 71/218 (32%), Positives = 105/218 (48%)
Frame = +3
Query: 9 CGGTLLNGNTVLTAAHCVWDMRNWDVNTFKYYQMKVYMGEYDLYRMYGVEKIFQVTKTII 188
CGG L+ VLTAAHC D KY V +G++ L + E+ QV ++I
Sbjct: 58 CGGVLVGDRWVLTAAHCKKD---------KY---SVRLGDHSLQKRDEPEQEIQVARSIQ 105
Query: 189 HPSYRHEYAYLEGNDVALIKFDGYISKNQYDYIDYNELDVNAISKPGDRCEVVGWGSMLP 368
HP + +D+ LI+ S N D + EL N K G +C + GWG++
Sbjct: 106 HPCFNSSNPEDHSHDIMLIRLQN--SANLGDKVKPIEL-ANLCPKVGQKCIISGWGTVTS 162
Query: 369 PGQDTSRFLQKAAINIISNSECGRIFSNVAYVRSNICAGSGRLSTSSCRGDSGGPLFCTD 548
P ++ L A + I S ++C R + +CAGS +C+GDSGGPL C
Sbjct: 163 PQENFPNTLNCAEVKIYSQNKCERAYPG-KITEGMVCAGSSN-GADTCQGDSGGPLVCNG 220
Query: 549 GRKHHIQVGIVSYGTVPCGQPNVPSVYTRVSEVAGWIR 662
+ GI ++G+ PCG+P P VYT++ WI+
Sbjct: 221 -----VLQGITTWGSDPCGKPEKPGVYTKICRYTNWIK 253
>sp|P00765|TRYP_ASTFL Trypsin I
Length = 237
Score = 112 bits (280), Expect = 1e-24
Identities = 77/229 (33%), Positives = 114/229 (49%), Gaps = 6/229 (2%)
Frame = +3
Query: 3 HFCGGTLLNGNTVLTAAHCVW--DMRNWDVNTFKYYQMKVYMGEYDLYRMYGVEKIFQVT 176
HFCG ++ N N +TA HCV+ D N +++ GE D+ G E+ V+
Sbjct: 28 HFCGASIYNENYAITAGHCVYGDDYENPS-------GLQIVAGELDMSVNEGSEQTITVS 80
Query: 177 KTIIHPSYRHEYAYLEGNDVALIKFDGYISKNQYDYIDYNELDVNAISKPGDRCE----V 344
K I+H ++ ++ L ND++L+K G ++ N N + A+ G V
Sbjct: 81 KIILHENFDYD---LLDNDISLLKLSGSLTFN-------NNVAPIALPAQGHTATGNVIV 130
Query: 345 VGWGSMLPPGQDTSRFLQKAAINIISNSECGRIFSNVAYVRSNICAGSGRLSTSSCRGDS 524
GWG+ G +T LQK + ++S++EC + S ICAG SC+GDS
Sbjct: 131 TGWGTT-SEGGNTPDVLQKVTVPLVSDAECRDDYGADEIFDSMICAGVPEGGKDSCQGDS 189
Query: 525 GGPLFCTDGRKHHIQVGIVSYGTVPCGQPNVPSVYTRVSEVAGWIRANA 671
GGPL +D ++ GIVS+G C +P P VYT VS WI+ANA
Sbjct: 190 GGPLAASDTGSTYL-AGIVSWG-YGCARPGYPGVYTEVSYHVDWIKANA 236
>sp|P26262|KLKB1_MOUSE Plasma kallikrein precursor (Plasma prekallikrein) (Kininogenin)
(Fletcher factor) [Contains: Plasma kallikrein heavy
chain; Plasma kallikrein light chain]
Length = 638
Score = 110 bits (276), Expect = 3e-24
Identities = 77/222 (34%), Positives = 109/222 (49%), Gaps = 3/222 (1%)
Frame = +3
Query: 3 HFCGGTLLNGNTVLTAAHCVWDMRNWDVNTFKYYQMKVYMGEYDLYRMYGVEKIFQVTKT 182
H CGG+++ VLTAAHC + DV ++Y G L + ++ +
Sbjct: 417 HLCGGSIIGRQWVLTAAHCFDGIPYPDV-------WRIYGGILSLSEITKETPSSRIKEL 469
Query: 183 IIHPSYRHEYAYLEGN-DVALIKFDGYISKNQYD--YIDYNELDVNAISKPGDRCEVVGW 353
IIH EY EGN D+ALIK ++ ++ ++ D N I C V GW
Sbjct: 470 IIH----QEYKVSEGNYDIALIKLQTPLNYTEFQKPICLPSKADTNTIYT---NCWVTGW 522
Query: 354 GSMLPPGQDTSRFLQKAAINIISNSECGRIFSNVAYVRSNICAGSGRLSTSSCRGDSGGP 533
G G+ T LQKA I ++ N EC + + + + ICAG T +C+GDSGGP
Sbjct: 523 GYTKEQGE-TQNILQKATIPLVPNEECQKKYRDYVINKQMICAGYKEGGTDACKGDSGGP 581
Query: 534 LFCTDGRKHHIQVGIVSYGTVPCGQPNVPSVYTRVSEVAGWI 659
L C + + VGI S+G CG+ + P VYT+VSE WI
Sbjct: 582 LVCKHSGRWQL-VGITSWGE-GCGRKDQPGVYTKVSEYMDWI 621
>sp|Q91VE3|KLK7_MOUSE Kallikrein-7 precursor (Stratum corneum chymotryptic enzyme)
(Thymopsin)
Length = 249
Score = 110 bits (275), Expect = 4e-24
Identities = 72/217 (33%), Positives = 110/217 (50%)
Frame = +3
Query: 9 CGGTLLNGNTVLTAAHCVWDMRNWDVNTFKYYQMKVYMGEYDLYRMYGVEKIFQVTKTII 188
CGG L++ VLTAAHC K Q +V +G D +KI + TK+
Sbjct: 51 CGGVLVDKYWVLTAAHC------------KMGQYQVQLGS-DKIGDQSAQKI-KATKSFR 96
Query: 189 HPSYRHEYAYLEGNDVALIKFDGYISKNQYDYIDYNELDVNAISKPGDRCEVVGWGSMLP 368
HP Y + ND+ L++ D + + ++ +L + PG C V GWG+
Sbjct: 97 HPGYSTK---THVNDIMLVRLDEPVKMSSK--VEAVQLPEHC-EPPGTSCTVSGWGTTTS 150
Query: 369 PGQDTSRFLQKAAINIISNSECGRIFSNVAYVRSNICAGSGRLSTSSCRGDSGGPLFCTD 548
P L + + +IS+ EC +++ ++ ++ +CAG T++C GDSGGPL C D
Sbjct: 151 PDVTFPSDLMCSDVKLISSRECKKVYKDLLG-KTMLCAGIPDSKTNTCNGDSGGPLVCND 209
Query: 549 GRKHHIQVGIVSYGTVPCGQPNVPSVYTRVSEVAGWI 659
+ G+VS+GT PCGQPN P VYT+V + W+
Sbjct: 210 TLQ-----GLVSWGTYPCGQPNDPGVYTQVCKYKRWV 241
>sp|P47796|CTRA_GADMO Chymotrypsin A precursor
Length = 263
Score = 110 bits (274), Expect = 5e-24
Identities = 73/220 (33%), Positives = 110/220 (50%), Gaps = 1/220 (0%)
Frame = +3
Query: 3 HFCGGTLLNGNTVLTAAHCVWDMRNWDVNTFKYYQMKVYMGEYDLYRMYGVEKIFQVTKT 182
HFCGG+L+N N V+TAAHC N Y+ +V +GE+D ++ V +
Sbjct: 58 HFCGGSLINENWVVTAAHC---------NVKNYH--RVVLGEHDRSSNSEGVQVMTVGQV 106
Query: 183 IIHPSYRHEYAYLEGNDVALIKFDGYISKN-QYDYIDYNELDVNAISKPGDRCEVVGWGS 359
HP Y + ND+ L+K + N + + E D + + G +C GWG
Sbjct: 107 FKHPRYN---GFTINNDILLVKLATPATLNMRVSPVCLAETD--DVFEGGMKCVTSGWGL 161
Query: 360 MLPPGQDTSRFLQKAAINIISNSECGRIFSNVAYVRSNICAGSGRLSTSSCRGDSGGPLF 539
DT LQ+AA+ +++N +C + + N S++ +G SSC GDSGGPL
Sbjct: 162 TRYNAADTPALLQQAALPLLTNEQCKKFWGNKI---SDLMICAGAAGASSCMGDSGGPLV 218
Query: 540 CTDGRKHHIQVGIVSYGTVPCGQPNVPSVYTRVSEVAGWI 659
C + VGIVS+G+ C P +P VY RV+E+ W+
Sbjct: 219 CQKAGSWTL-VGIVSWGSGTC-TPTMPGVYARVTELRAWV 256
>sp|P15949|KLK9_MOUSE Glandular kallikrein K9 precursor (Tissue kallikrein 9) (mGK-9)
(Epidermal growth factor-binding protein type C) (EGF-BP
C)
Length = 261
Score = 108 bits (270), Expect = 1e-23
Identities = 78/240 (32%), Positives = 111/240 (46%), Gaps = 20/240 (8%)
Frame = +3
Query: 3 HFCGGTLLNGNTVLTAAHCVWDMRNWDVNTFKYYQMKVYMGEYDLYRMYGVEKIFQVTKT 182
+ CGG LL+ N VLTAAHC Y + KV +G+ +LY + V+K+
Sbjct: 48 YICGGVLLDANWVLTAAHCY------------YEENKVSLGKNNLYEEEPSAQHRLVSKS 95
Query: 183 IIHPSYRHEYAYLEGNDVALIKFDGYISKNQYDYIDYNELDVNAISKPGDRCEVV----- 347
+HP Y L N +I +YDY N+L + +SKP D +VV
Sbjct: 96 FLHPGYNRS---LHRN---------HIRHPEYDYS--NDLMLLRLSKPADITDVVKPIAL 141
Query: 348 --------------GWGSMLPPGQDTSRFLQKAAINIISNSECGRI-FSNVAYVRSNICA 482
GWGS P ++ LQ + ++ N +CG+ V V +CA
Sbjct: 142 PTEEPKLGSTCLASGWGSTTPFKFQNAKDLQCVNLKLLPNEDCGKAHIEKVTDVM--LCA 199
Query: 483 GSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQPNVPSVYTRVSEVAGWIR 662
G +C+GDSGGPL C DG + GI S+G PCG+P P VYT++ + WI+
Sbjct: 200 GETDGGKDTCKGDSGGPLIC-DG----VLQGITSWGFTPCGEPKKPGVYTKLIKFTSWIK 254
>sp|Q9JHJ7|TEST_MOUSE Testisin precursor (Tryptase 4)
Length = 324
Score = 108 bits (269), Expect = 2e-23
Identities = 82/235 (34%), Positives = 117/235 (49%), Gaps = 14/235 (5%)
Frame = +3
Query: 3 HFCGGTLLNGNTVLTAAHCVW---DMRNWDVNTFKYYQMKVYMGEYDLYRMYGVEKIFQV 173
H CG TLLN VLTAAHC D +W V Q L+ + +Q+
Sbjct: 78 HLCGATLLNRRWVLTAAHCFQKDNDPFDWTV------QFGELTSRPSLWNLQAYSNRYQI 131
Query: 174 TKTIIHPSYRHEYAYLEGNDVALIKFDGYISKNQYDYIDYNELDVNAISKPGDR--CEVV 347
+ P Y +Y ND+AL+K ++ N ++I L +N+ K +R C V
Sbjct: 132 EDIFLSPKYSEQYP----NDIALLKLSSPVTYN--NFIQPICL-LNSTYKFENRTDCWVT 184
Query: 348 GWGSMLPPGQDTS----RFLQKAAINIISNSECGRIFSNVAYVRSNI-----CAGSGRLS 500
GWG++ G+D S LQ+ + II+NS C ++ + R+NI CAG+
Sbjct: 185 GWGAI---GEDESLPSPNTLQEVQVAIINNSMCNHMYKKPDF-RTNIWGDMVCAGTPEGG 240
Query: 501 TSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQPNVPSVYTRVSEVAGWIRA 665
+C GDSGGPL C D QVG+VS+G + CG+PN P VYT +S WI++
Sbjct: 241 KDACFGDSGGPLAC-DQDTVWYQVGVVSWG-IGCGRPNRPGVYTNISHHYNWIQS 293
>sp|P36368|KLK13_MOUSE Glandular kallikrein K13 precursor (Tissue kallikrein 13) (mGK-13)
(Epidermal growth factor-binding protein type B) (EGF-BP
B) (Prorenin-converting enzyme) (preCE)
Length = 261
Score = 108 bits (269), Expect = 2e-23
Identities = 75/229 (32%), Positives = 107/229 (46%), Gaps = 9/229 (3%)
Frame = +3
Query: 3 HFCGGTLLNGNTVLTAAHCVWDMRNWDVNTFKYYQMKVYMGEYDLYRMYGVEKIFQVTKT 182
H CGG LL+ N VLTAAHC D Q +V++G+ L++ + V+K+
Sbjct: 48 HICGGVLLDRNWVLTAAHCYVD------------QYEVWLGKNKLFQEEPSAQHRLVSKS 95
Query: 183 IIHPSYRHEYAYLE--------GNDVALIKFDGYISKNQYDYIDYNELDVNAISKPGDRC 338
HP + L+ ND+ L++ + D + L KPG +C
Sbjct: 96 FPHPGFNMSLLMLQTIPPGADFSNDLMLLRLSK--PADITDVVKPIALPTKE-PKPGSKC 152
Query: 339 EVVGWGSMLPPGQDTSRFLQKAAINIISNSECGRIF-SNVAYVRSNICAGSGRLSTSSCR 515
GWGS+ P LQ I ++ N C +++ V V +CAG +CR
Sbjct: 153 LASGWGSITPTRWQKPDDLQCVFITLLPNENCAKVYLQKVTDVM--LCAGEMGGGKDTCR 210
Query: 516 GDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQPNVPSVYTRVSEVAGWIR 662
DSGGPL C DG I G SYG VPCG+P VP++YT + + WI+
Sbjct: 211 DDSGGPLIC-DG----ILQGTTSYGPVPCGKPGVPAIYTNLIKFNSWIK 254
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 88,039,899
Number of Sequences: 369166
Number of extensions: 1894824
Number of successful extensions: 6547
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5240
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5448
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6462248555
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)