Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_021_A08
(867 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q96T60|PNKP_HUMAN Bifunctional polynucleotide phosphatas... 193 6e-49
sp|Q9JLV6|PNKP_MOUSE Bifunctional polynucleotide phosphatas... 189 7e-48
sp|Q19683|YZR5_CAEEL Hypothetical protein F21D5.5 in chromo... 180 5e-45
sp|O13911|PNK1_SCHPO Bifunctional polynucleotide phosphatas... 100 4e-21
sp|P21286|Y033_NPVAC Hypothetical 20.8 kDa protein in FGF-V... 62 2e-09
sp|Q03796|TPP1_YEAST Polynucleotide 3'-phosphatase (2'(3')-... 47 7e-05
sp|Q9HET9|TPP1_SACMI Polynucleotide 3'-phosphatase (2'(3')-... 39 0.019
sp|Q9Y6K8|KAD5_HUMAN Adenylate kinase isoenzyme 5 (ATP-AMP ... 34 0.60
sp|P06855|KIPN_BPT4 Polynucleotide kinase (PNK) (Polynucleo... 33 1.0
sp|P41476|PNKL_NPVAC Putative bifunctional polynucleotide k... 33 1.3
>sp|Q96T60|PNKP_HUMAN Bifunctional polynucleotide phosphatase/kinase (Polynucleotide
kinase-3'-phosphatase) (DNA 5'-kinase/3'-phosphatase)
[Includes: Polynucleotide 3'-phosphatase
(2'(3')-polynucleotidase); Polynucleotide
5'-hydroxyl-kinase ]
Length = 521
Score = 193 bits (490), Expect = 6e-49
Identities = 113/273 (41%), Positives = 159/273 (58%), Gaps = 1/273 (0%)
Frame = +3
Query: 45 EQLAVKIQVLVATNYSNVYRKPNTGMFDLLDKEHNSGIKINCDESLYVGDAAGRVADWAP 224
E+L V QVLVAT ++ +YRKP TGM+D L ++ N G I+ +S++VGDAAGR A+WAP
Sbjct: 241 EKLGVPFQVLVAT-HAGLYRKPVTGMWDHLQEQANDGTPISIGDSIFVGDAAGRPANWAP 299
Query: 225 -KMKKDFSNSDRLFALNCHLKFYTPEAFFLQQEEGEFKMPEFHPAETVKNCPTLMIKYDK 401
+ KKDFS +DRLFALN L F TPE FFL+ F++P F P TV L + +
Sbjct: 300 GRKKKDFSCADRLFALNLGLPFATPEEFFLKWPAAGFELPAFDP-RTVSRSGPLCLPESR 358
Query: 402 FQKSASDIKDILPKDSSEVVLILMVGYPGSGKSTFCKKYLEPLGFNVINRDTLKTWQKCV 581
SAS +++ VG+PG+GKSTF KK+L G+ +NRDTL +WQ+CV
Sbjct: 359 ALLSASP------------EVVVAVGFPGAGKSTFLKKHLVSAGYVHVNRDTLGSWQRCV 406
Query: 582 AETQSLFXXXXXXXXXXTNPDLATRDRYISAAVECGVKHIICCIMDVSEDQSRHNEHFRQ 761
++ TNPD A+R RY+ A GV C + + +Q+RHN FR+
Sbjct: 407 TTCETALKQGKRVAIDNTNPDAASRARYVQCARAAGVP-CRCFLFTATLEQARHNNRFRE 465
Query: 762 LTADHHDHVSEMVFKIHKNKYVEPSDKEKFTEI 860
+T H VS+MV ++ ++ P+ E F+ I
Sbjct: 466 MTDSSHIPVSDMVMYGYRKQFEAPTLAEGFSAI 498
>sp|Q9JLV6|PNKP_MOUSE Bifunctional polynucleotide phosphatase/kinase (Polynucleotide
kinase-3'-phosphatase) (DNA 5'-kinase/3'-phosphatase)
[Includes: Polynucleotide 3'-phosphatase
(2'(3')-polynucleotidase); Polynucleotide
5'-hydroxyl-kinase ]
Length = 522
Score = 189 bits (481), Expect = 7e-48
Identities = 114/273 (41%), Positives = 166/273 (60%), Gaps = 1/273 (0%)
Frame = +3
Query: 45 EQLAVKIQVLVATNYSNVYRKPNTGMFDLLDKEHNSGIKINCDESLYVGDAAGRVADWAP 224
E+L V QVLVAT ++ + RKP +GM+D L ++ N GI I+ ++S++VGDAAGR+A+WAP
Sbjct: 240 EKLGVPFQVLVAT-HAGLNRKPVSGMWDHLQEQANEGIPISVEDSVFVGDAAGRLANWAP 298
Query: 225 -KMKKDFSNSDRLFALNCHLKFYTPEAFFLQQEEGEFKMPEFHPAETVKNCPTLMIKYDK 401
+ KKDFS +DRLFALN L F TPE FFL+ F++P F P T+ + L +
Sbjct: 299 GRKKKDFSCADRLFALNVGLPFATPEEFFLKWPAARFELPAFDP-RTISSAGPLYLP--- 354
Query: 402 FQKSASDIKDILPKDSSEVVLILMVGYPGSGKSTFCKKYLEPLGFNVINRDTLKTWQKCV 581
+S+S L + EVV+ VG+PG+GKSTF +++L G+ +NRDTL +WQ+CV
Sbjct: 355 --ESSS-----LLSPNPEVVVA--VGFPGAGKSTFIQEHLVSAGYVHVNRDTLGSWQRCV 405
Query: 582 AETQSLFXXXXXXXXXXTNPDLATRDRYISAAVECGVKHIICCIMDVSEDQSRHNEHFRQ 761
+ Q+ TNPD+ +R RYI A + GV C + +Q+RHN FR+
Sbjct: 406 SSCQAALRQGKRVVIDNTNPDVPSRARYIQCAKDAGVP-CRCFNFCATIEQARHNNRFRE 464
Query: 762 LTADHHDHVSEMVFKIHKNKYVEPSDKEKFTEI 860
+T H VS+MV ++ ++ P+ E F EI
Sbjct: 465 MTDPSHAPVSDMVMFSYRKQFEPPTLAEGFLEI 497
>sp|Q19683|YZR5_CAEEL Hypothetical protein F21D5.5 in chromosome IV
Length = 407
Score = 180 bits (456), Expect = 5e-45
Identities = 115/277 (41%), Positives = 157/277 (56%), Gaps = 4/277 (1%)
Frame = +3
Query: 48 QLAVKIQVLVATNYSNVYRKPNTGMFDLLDKEHNSGIKINCDESLYVGDAAGRVADWAPK 227
+L + +Q V+ + YRKP GM++ L K N ++IN ES++VGDAAGR+ + +
Sbjct: 128 KLGIPVQAFVSVAGGH-YRKPCVGMWNEL-KLRNDEVEINEKESIFVGDAAGRIKTTS-R 184
Query: 228 MKKDFSNSDRLFALNCHLKFYTPEAFFLQQEEGEFKMPEFHPAETVKNCPTLMIKYDKFQ 407
KKD S +DR FA N +KF TPE FF G+ K+ E P +D
Sbjct: 185 PKKDHSYADRFFAANVGVKFQTPEEFF-----GKSKVDE----------PWGPPNFDPKN 229
Query: 408 KSASDIKDILPKD----SSEVVLILMVGYPGSGKSTFCKKYLEPLGFNVINRDTLKTWQK 575
+ +I ++ P D SSE +ILMVG+PGSGKSTF K + ++NRDT+ TWQK
Sbjct: 230 LFSEEITELEPHDAQLKSSEKEIILMVGFPGSGKSTFAKMLGHQHDYKIVNRDTIGTWQK 289
Query: 576 CVAETQSLFXXXXXXXXXXTNPDLATRDRYISAAVECGVKHIICCIMDVSEDQSRHNEHF 755
CVA T+S T+PDL +R RYI A E GV I C M+ S + ++HN F
Sbjct: 290 CVAATRSYLADGKSVVIDNTSPDLESRKRYIDVAKELGVP-IRCFEMNCSMEHAQHNIRF 348
Query: 756 RQLTADHHDHVSEMVFKIHKNKYVEPSDKEKFTEICK 866
R LT D+ +S MV +IHK KYVEP+ E F++I K
Sbjct: 349 RVLTDDNAAEISSMVLRIHKGKYVEPTLSEGFSQIVK 385
>sp|O13911|PNK1_SCHPO Bifunctional polynucleotide phosphatase/kinase (Polynucleotide
kinase-3'-phosphatase) (DNA 5'-kinase/3'-phosphatase)
[Includes: Polynucleotide 3'-phosphatase
(2'(3')-polynucleotidase); Polynucleotide
5'-hydroxy-kinase ]
Length = 421
Score = 100 bits (250), Expect = 4e-21
Identities = 83/280 (29%), Positives = 128/280 (45%), Gaps = 16/280 (5%)
Frame = +3
Query: 69 VLVATNYSNVYRKPNTGMFDLLDKEHNSGIKINCDESLYVGDAAGRVADWAPKMKKDFSN 248
VL A + +RKP TGM++ K+ N I ++ + YVGDAAGR D ++
Sbjct: 151 VLYAAILKDKFRKPLTGMWNSFLKDVNRSIDLSFIK--YVGDAAGRPGD--------HNS 200
Query: 249 SDRLFALNCHLKFYTPEAFFLQQEEGEFKMPEFHPAE-TVKNCPTLMIKYDKFQKSASDI 425
+D FA N +KF TPE FFL FHP V+N + + K
Sbjct: 201 TDLKFAENIGIKFETPEQFFLGHSFVPPNFESFHPKNYLVRNSSSHPYHFKK-------- 252
Query: 426 KDILPKDSSEVVLILMVGYPGSGKSTFCKKYLEPLGFNVINRDTLKTWQKCV-AETQSL- 599
S ++++VG+P SGKST + + G+ +N+D LKT KC+ A ++L
Sbjct: 253 -------SEHQEIVVLVGFPSSGKSTLAESQIVTQGYERVNQDILKTKSKCIKAAIEALK 305
Query: 600 -----------FXXXXXXXXXXTNPDLATRDRYISAAVECGVKHIICCIMDVSEDQSRHN 746
TNP + +R +I A E + I C + SE+ +RHN
Sbjct: 306 KEKSVVIGMYSIISTTYAISDNTNPTIESRKMWIDIAQEFEIP-IRCIHLQSSEELARHN 364
Query: 747 EHFRQLTADHHD--HVSEMVFKIHKNKYVEPSDKEKFTEI 860
FR + HH+ + E+ F K+++ P+ +E FT +
Sbjct: 365 NVFRYI---HHNQKQLPEIAFNSFKSRFQMPTVEEGFTNV 401
>sp|P21286|Y033_NPVAC Hypothetical 20.8 kDa protein in FGF-VUBI intergenic region (ORF 1)
Length = 182
Score = 62.4 bits (150), Expect = 2e-09
Identities = 38/83 (45%), Positives = 53/83 (63%)
Frame = +3
Query: 57 VKIQVLVATNYSNVYRKPNTGMFDLLDKEHNSGIKINCDESLYVGDAAGRVADWAPKMKK 236
V I V+ N + +RKP+TGM+ + K+ I+ ++S YVGDAAGR+ +K
Sbjct: 99 VPISFYVSPN-KDEHRKPDTGMWREMAKQFTH---IDKEQSFYVGDAAGRIN--LTTGQK 152
Query: 237 DFSNSDRLFALNCHLKFYTPEAF 305
DFS+SDR+FA N L+FYTPE F
Sbjct: 153 DFSDSDRVFAKNLSLQFYTPEQF 175
>sp|Q03796|TPP1_YEAST Polynucleotide 3'-phosphatase (2'(3')-polynucleotidase) (DNA 3'
phosphatase) (Three prime phosphatase)
Length = 238
Score = 47.0 bits (110), Expect = 7e-05
Identities = 30/76 (39%), Positives = 39/76 (51%), Gaps = 7/76 (9%)
Frame = +3
Query: 102 RKPNTGMFDLLDKEHNSGIKINCDES-------LYVGDAAGRVADWAPKMKKDFSNSDRL 260
RKP TGM + ++ S +++ S Y GDAAGR KKDFS+SD
Sbjct: 169 RKPMTGMVEFFKRDLESAYRVSEQISPIKLNWIYYCGDAAGR--------KKDFSDSDIK 220
Query: 261 FALNCHLKFYTPEAFF 308
FA N H++F PE F
Sbjct: 221 FAENLHVEFKYPEEIF 236
>sp|Q9HET9|TPP1_SACMI Polynucleotide 3'-phosphatase (2'(3')-polynucleotidase) (DNA 3'
phosphatase) (Three prime phosphatase)
Length = 245
Score = 38.9 bits (89), Expect = 0.019
Identities = 28/76 (36%), Positives = 34/76 (44%), Gaps = 7/76 (9%)
Frame = +3
Query: 102 RKPNTGMFDLLDKEHNSGIK-------INCDESLYVGDAAGRVADWAPKMKKDFSNSDRL 260
RKP TGM D + + I + Y GDAAGR K DFS+SD
Sbjct: 176 RKPMTGMADFFRIDITDACRVLKTVPPIKLNWVYYCGDAAGR--------KNDFSDSDIK 227
Query: 261 FALNCHLKFYTPEAFF 308
FA H++F PE F
Sbjct: 228 FAEKLHVEFKYPEEVF 243
>sp|Q9Y6K8|KAD5_HUMAN Adenylate kinase isoenzyme 5 (ATP-AMP transphosphorylase)
Length = 198
Score = 33.9 bits (76), Expect = 0.60
Identities = 15/40 (37%), Positives = 24/40 (60%)
Frame = +3
Query: 441 KDSSEVVLILMVGYPGSGKSTFCKKYLEPLGFNVINRDTL 560
+D + +I ++G PGSGK T C+K +E GF ++ L
Sbjct: 6 EDLRKCKIIFIIGGPGSGKGTQCEKLVEKYGFTHLSTGEL 45
>sp|P06855|KIPN_BPT4 Polynucleotide kinase (PNK) (Polynucleotide 5'-hydroxy-kinase)
Length = 301
Score = 33.1 bits (74), Expect = 1.0
Identities = 16/32 (50%), Positives = 24/32 (75%), Gaps = 1/32 (3%)
Frame = +3
Query: 462 LILMVGYPGSGKSTFCKKYL-EPLGFNVINRD 554
+IL +G PGSGKST+ ++++ + GF INRD
Sbjct: 4 IILTIGCPGSGKSTWAREFIAKNPGFYNINRD 35
>sp|P41476|PNKL_NPVAC Putative bifunctional polynucleotide kinase/RNA ligase [Includes:
Polynucleotide kinase (PNK) (Polynucleotide
5'-hydroxy-kinase); RNA ligase ]
Length = 694
Score = 32.7 bits (73), Expect = 1.3
Identities = 25/82 (30%), Positives = 43/82 (52%), Gaps = 1/82 (1%)
Frame = +3
Query: 321 EGEF-KMPEFHPAETVKNCPTLMIKYDKFQKSASDIKDILPKDSSEVVLILMVGYPGSGK 497
E EF K+ E + +KN +IK+ +++ ++ K S L++++G GSGK
Sbjct: 352 ESEFLKLTEPEKIKMIKN----IIKFVSTKQALNNKLAPTIKLPSSKQLLVLIGISGSGK 407
Query: 498 STFCKKYLEPLGFNVINRDTLK 563
ST+ K G+ INRD ++
Sbjct: 408 STYAKSL---KGYTEINRDDVR 426
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 100,195,135
Number of Sequences: 369166
Number of extensions: 2098411
Number of successful extensions: 6464
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6215
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6448
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8550075140
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)