Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_020_P19
(579 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O76062|ERG24_HUMAN Delta(14)-sterol reductase (C-14 ster... 121 1e-27
sp|Q14739|LBR_HUMAN Lamin-B receptor (Integral nuclear enve... 119 5e-27
sp|Q09195|ERG24_SCHPO Delta(14)-sterol reductase (C-14 ster... 111 1e-24
sp|P23913|LBR_CHICK Lamin-B receptor 105 6e-23
sp|P78575|ERG24_ASCIM Delta(14)-sterol reductase (C-14 ster... 105 8e-23
sp|P32462|ERG24_YEAST Delta(14)-sterol reductase (C-14 ster... 97 2e-20
sp|Q9LDR4|ERG24_ARATH Delta(14)-sterol reductase (C-14 ster... 92 1e-18
sp|P38670|ERG24_NEUCR Delta(14)-sterol reductase (C-14 ster... 88 1e-17
sp|Q9UBM7|DHCR7_HUMAN 7-dehydrocholesterol reductase (7-DHC... 87 4e-17
sp|O88455|DHCR7_MOUSE 7-dehydrocholesterol reductase (7-DHC... 84 2e-16
>sp|O76062|ERG24_HUMAN Delta(14)-sterol reductase (C-14 sterol reductase) (Sterol
C14-reductase) (Delta14-SR) (Transmembrane 7 superfamily
member 2) (Another new gene 1 protein) (Putative sterol
reductase SR-1)
Length = 418
Score = 121 bits (304), Expect = 1e-27
Identities = 58/134 (43%), Positives = 87/134 (64%)
Frame = +3
Query: 33 LPIKCLIGIGAINFIGYIIYRTSNIQKNSFRKNPYNVHLCHLKTLTNRHGRQLLVGGWWG 212
LP+ +I + IN IGY I+R +N QKN+FRKNP + + L+T++ GR+LLV GWWG
Sbjct: 287 LPMASVICL--INAIGYYIFRGANSQKNTFRKNPSDPRVAGLETISTATGRKLLVSGWWG 344
Query: 213 WLRHPNYLGDIIMSLSWSLCTGLHSGVCWXXXXXXXXXXXXRCIQDDRKCAIEHETTWSE 392
+RHPNYLGD+IM+L+WSL G+ + + R +D+R+C ++ W E
Sbjct: 345 MVRHPNYLGDLIMALAWSLPCGVSHLLPYFYLLYFTALLVHREARDERQCLQKYGLAWQE 404
Query: 393 YRKAVPYRLIPFIF 434
Y + VPYR++P+I+
Sbjct: 405 YCRRVPYRIMPYIY 418
>sp|Q14739|LBR_HUMAN Lamin-B receptor (Integral nuclear envelope inner membrane protein)
(LMN2R)
Length = 615
Score = 119 bits (298), Expect = 5e-27
Identities = 56/138 (40%), Positives = 84/138 (60%)
Frame = +3
Query: 21 NKFELPIKCLIGIGAINFIGYIIYRTSNIQKNSFRKNPYNVHLCHLKTLTNRHGRQLLVG 200
N+ P+ LI + + GY+I+R +N QKN+FRKNP + L HLKT+ G+ LLV
Sbjct: 480 NEVSWPMASLIIV--LKLCGYVIFRGANSQKNAFRKNPSDPKLAHLKTIHTSTGKNLLVS 537
Query: 201 GWWGWLRHPNYLGDIIMSLSWSLCTGLHSGVCWXXXXXXXXXXXXRCIQDDRKCAIEHET 380
GWWG++RHPNYLGD+IM+L+WSL G + + + R +D+ C ++
Sbjct: 538 GWWGFVRHPNYLGDLIMALAWSLPCGFNHILPYFYIIYFTMLLVHREARDEYHCKKKYGV 597
Query: 381 TWSEYRKAVPYRLIPFIF 434
W +Y + VPYR+ P+I+
Sbjct: 598 AWEKYCQRVPYRIFPYIY 615
>sp|Q09195|ERG24_SCHPO Delta(14)-sterol reductase (C-14 sterol reductase) (Sterol
C14-reductase)
Length = 424
Score = 111 bits (278), Expect = 1e-24
Identities = 53/143 (37%), Positives = 79/143 (55%)
Frame = +3
Query: 6 RHEEINKFELPIKCLIGIGAINFIGYIIYRTSNIQKNSFRKNPYNVHLCHLKTLTNRHGR 185
R+ + +L + + I + F+GY I+R +N QKN FR NP + L HLK + + G
Sbjct: 282 RYLAFHPVDLGLVKTLAILCLQFLGYYIFRGANGQKNRFRSNPNDPKLKHLKFIQTKRGT 341
Query: 186 QLLVGGWWGWLRHPNYLGDIIMSLSWSLCTGLHSGVCWXXXXXXXXXXXXRCIQDDRKCA 365
+LL GWWG RH NY GD IM+ +W L G S + + R +DD KC
Sbjct: 342 KLLTSGWWGMARHINYFGDWIMAWAWCLPAGFGSPIPYFYVAYFGVLLVHRNARDDHKCR 401
Query: 366 IEHETTWSEYRKAVPYRLIPFIF 434
+++ W +Y KAV YR+IP+++
Sbjct: 402 VKYGEDWEKYCKAVKYRIIPYVY 424
>sp|P23913|LBR_CHICK Lamin-B receptor
Length = 637
Score = 105 bits (263), Expect = 6e-23
Identities = 50/119 (42%), Positives = 72/119 (60%)
Frame = +3
Query: 57 IGAINFIGYIIYRTSNIQKNSFRKNPYNVHLCHLKTLTNRHGRQLLVGGWWGWLRHPNYL 236
I +N IGY I+R++N QKN+FR+NP + L +LK + G+ LLV GWWG++RHPNYL
Sbjct: 483 ITILNCIGYYIFRSANSQKNNFRRNPADPKLSYLKVIPTATGKGLLVTGWWGFVRHPNYL 542
Query: 237 GDIIMSLSWSLCTGLHSGVCWXXXXXXXXXXXXRCIQDDRKCAIEHETTWSEYRKAVPY 413
GDIIM+L+WSL G + + + R +D+ C ++ W Y + VPY
Sbjct: 543 GDIIMALAWSLPCGFNHILPYFYVIYFICLLVHREARDEHHCKKKYGLAWERYCQRVPY 601
>sp|P78575|ERG24_ASCIM Delta(14)-sterol reductase (C-14 sterol reductase) (Sterol
C14-reductase)
Length = 430
Score = 105 bits (262), Expect = 8e-23
Identities = 50/129 (38%), Positives = 75/129 (58%)
Frame = +3
Query: 48 LIGIGAINFIGYIIYRTSNIQKNSFRKNPYNVHLCHLKTLTNRHGRQLLVGGWWGWLRHP 227
+ G+ A+ F GY I+R +N QKN+FR NP + + HLK +T + G +LL+ GWWG RH
Sbjct: 302 IAGVLAVQFTGYAIFRGANNQKNAFRTNPADPAVSHLKFMTTKSGSKLLISGWWGVARHV 361
Query: 228 NYLGDIIMSLSWSLCTGLHSGVCWXXXXXXXXXXXXRCIQDDRKCAIEHETTWSEYRKAV 407
NY GD IM+ S+ L TG ++ + + R +D+ KC ++ W Y K V
Sbjct: 362 NYFGDWIMAWSYCLTTGFNTPLTYFYVIYFGILLLHRDRRDEAKCREKYGKDWDRYCKVV 421
Query: 408 PYRLIPFIF 434
+R+IP I+
Sbjct: 422 KWRIIPGIY 430
>sp|P32462|ERG24_YEAST Delta(14)-sterol reductase (C-14 sterol reductase) (Sterol
C14-reductase)
Length = 438
Score = 97.4 bits (241), Expect = 2e-20
Identities = 47/143 (32%), Positives = 81/143 (56%)
Frame = +3
Query: 6 RHEEINKFELPIKCLIGIGAINFIGYIIYRTSNIQKNSFRKNPYNVHLCHLKTLTNRHGR 185
R+ ++ EL ++GI AI F+G+ I+ ++N QK+ FR+ L +LK++ + G
Sbjct: 300 RYLSVSPVELGWVKVVGILAIMFLGFHIFHSANKQKSEFRQGK----LENLKSIQTKRGT 355
Query: 186 QLLVGGWWGWLRHPNYLGDIIMSLSWSLCTGLHSGVCWXXXXXXXXXXXXRCIQDDRKCA 365
+LL GWW +H NY GD ++SLSW L T + + + R +D+ KC
Sbjct: 356 KLLCDGWWAKSQHINYFGDWLISLSWCLATWFQTPLTYYYSLYFATLLLHRQQRDEHKCR 415
Query: 366 IEHETTWSEYRKAVPYRLIPFIF 434
+++ W EY + VPY++IP+++
Sbjct: 416 LKYGENWEEYERKVPYKIIPYVY 438
>sp|Q9LDR4|ERG24_ARATH Delta(14)-sterol reductase (C-14 sterol reductase) (Sterol
C14-reductase) (FACKEL protein)
Length = 369
Score = 91.7 bits (226), Expect = 1e-18
Identities = 47/138 (34%), Positives = 81/138 (58%)
Frame = +3
Query: 21 NKFELPIKCLIGIGAINFIGYIIYRTSNIQKNSFRKNPYNVHLCHLKTLTNRHGRQLLVG 200
NK EL + ++ + IGY+++R +N QK+ F+KNP + G +LLV
Sbjct: 235 NKVELTVPAIVVNCLVFLIGYMVFRGANKQKHIFKKNPKTPIWGKPPVVV---GGKLLVS 291
Query: 201 GWWGWLRHPNYLGDIIMSLSWSLCTGLHSGVCWXXXXXXXXXXXXRCIQDDRKCAIEHET 380
G+WG RH NYLGD++++LS+SL G+ S V + R +D+ +CA +++
Sbjct: 292 GYWGIARHCNYLGDLMLALSFSLPCGISSPVPYFYPIYLLILLIWRERRDEVRCAEKYKE 351
Query: 381 TWSEYRKAVPYRLIPFIF 434
W+EY + VP+R++P+++
Sbjct: 352 IWAEYLRLVPWRILPYVY 369
>sp|P38670|ERG24_NEUCR Delta(14)-sterol reductase (C-14 sterol reductase) (Sterol
C14-reductase)
Length = 490
Score = 88.2 bits (217), Expect = 1e-17
Identities = 54/175 (30%), Positives = 82/175 (46%), Gaps = 32/175 (18%)
Frame = +3
Query: 6 RHEEINKFELPIKCLIGIGAINFIGYIIYRTSNIQKNSFRKNPYNVHLCHLKTLTNRHGR 185
R+ ++ +L LI +GA+ GY I+R SN QKN+FR NP + + HL L + G
Sbjct: 316 RYLSVHPQQLGAFGLIAVGAVLAAGYSIFRLSNSQKNNFRTNPEDPSVKHLTYLQTKTGS 375
Query: 186 QLLVGGWWGWLRHPNYLGDIIMSLSWSLCTG------LHSG------------------- 290
+L+ GWWG RH NYLGD + S +SL TG L +G
Sbjct: 376 RLITSGWWGIARHINYLGDWLQSWPYSLPTGIAGYQILSAGSNAPGAITMLDGREVVQGE 435
Query: 291 -------VCWXXXXXXXXXXXXRCIQDDRKCAIEHETTWSEYRKAVPYRLIPFIF 434
+ R ++DD KC+ ++ W +Y+K V +R++P I+
Sbjct: 436 ARGWGIVFTYFYILYFAILLIHRDLRDDEKCSKKYGDDWEKYKKLVKWRIVPGIY 490
>sp|Q9UBM7|DHCR7_HUMAN 7-dehydrocholesterol reductase (7-DHC reductase) (Sterol
delta-7-reductase) (Putative sterol reductase SR-2)
Length = 475
Score = 86.7 bits (213), Expect = 4e-17
Identities = 45/138 (32%), Positives = 71/138 (51%), Gaps = 10/138 (7%)
Frame = +3
Query: 51 IGIGAINFIGYIIYRTSNIQKNSFRKN----------PYNVHLCHLKTLTNRHGRQLLVG 200
+G+ + +GY I+R +N QK+ FR+ P + + RH +LLV
Sbjct: 338 VGVLLLGLVGYYIFRVANHQKDLFRRTDGRCLIWGRKPKVIECSYTSADGQRHHSKLLVS 397
Query: 201 GWWGWLRHPNYLGDIIMSLSWSLCTGLHSGVCWXXXXXXXXXXXXRCIQDDRKCAIEHET 380
G+WG RH NY+GD++ SL++ L G + + RC++D+ +CA ++
Sbjct: 398 GFWGVARHFNYVGDLMGSLAYCLACGGGHLLPYFYIIYMAILLTHRCLRDEHRCASKYGR 457
Query: 381 TWSEYRKAVPYRLIPFIF 434
W Y AVPYRL+P IF
Sbjct: 458 DWERYTAAVPYRLLPGIF 475
>sp|O88455|DHCR7_MOUSE 7-dehydrocholesterol reductase (7-DHC reductase) (Sterol
delta-7-reductase)
Length = 471
Score = 84.3 bits (207), Expect = 2e-16
Identities = 46/138 (33%), Positives = 70/138 (50%), Gaps = 10/138 (7%)
Frame = +3
Query: 51 IGIGAINFIGYIIYRTSNIQKNSFR----------KNPYNVHLCHLKTLTNRHGRQLLVG 200
+GI + +GY I+R +N QK+ FR K P + + +H +LLV
Sbjct: 334 LGILLLGLVGYYIFRMTNHQKDLFRRTDGRCLIWGKKPKAIECSYTSADGLKHHSKLLVS 393
Query: 201 GWWGWLRHPNYLGDIIMSLSWSLCTGLHSGVCWXXXXXXXXXXXXRCIQDDRKCAIEHET 380
G+WG RH NY GD++ SL++ L G + + RC++D+ +CA ++
Sbjct: 394 GFWGVARHFNYTGDLMGSLAYCLACGGGHLLPYFYIIYMTILLTHRCLRDEHRCANKYGR 453
Query: 381 TWSEYRKAVPYRLIPFIF 434
W Y AVPYRL+P IF
Sbjct: 454 DWERYTAAVPYRLLPGIF 471
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 66,115,939
Number of Sequences: 369166
Number of extensions: 1350277
Number of successful extensions: 3179
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3051
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3166
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4259329035
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)