Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_020_P11
(612 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P11960|ODBA_RAT 2-oxoisovalerate dehydrogenase alpha sub... 121 2e-27
sp|P50136|ODBA_MOUSE 2-oxoisovalerate dehydrogenase alpha s... 121 2e-27
sp|Q8HXY4|ODBA_MACFA 2-oxoisovalerate dehydrogenase alpha s... 117 3e-26
sp|P12694|ODBA_HUMAN 2-oxoisovalerate dehydrogenase alpha s... 114 2e-25
sp|P11178|ODBA_BOVIN 2-oxoisovalerate dehydrogenase alpha s... 112 6e-25
sp|P35485|ODPA_ACHLA Pyruvate dehydrogenase E1 component, a... 53 5e-07
sp|P09060|ODBA_PSEPU 2-oxoisovalerate dehydrogenase alpha s... 53 7e-07
sp|P52901|ODPA_ARATH Pyruvate dehydrogenase E1 component al... 52 1e-06
sp|P52902|ODPA_PEA Pyruvate dehydrogenase E1 component alph... 52 2e-06
sp|P52903|ODPA_SOLTU Pyruvate dehydrogenase E1 component al... 48 2e-05
>sp|P11960|ODBA_RAT 2-oxoisovalerate dehydrogenase alpha subunit, mitochondrial
precursor (Branched-chain alpha-keto acid dehydrogenase
E1 component alpha chain) (BCKDH E1-alpha)
Length = 441
Score = 121 bits (303), Expect = 2e-27
Identities = 55/102 (53%), Positives = 73/102 (71%)
Frame = +1
Query: 1 TSDDSLAYRSVDEISWWDKHDNPISRLKQYLLNKEWWTEKEEVSWRHEARKLVVKAIEKT 180
TSDDS AYRSVDE+++WDK D+PISRL+QYLLN+ WW E++E +WR ++RK V++A E+
Sbjct: 334 TSDDSSAYRSVDEVNYWDKQDHPISRLRQYLLNQGWWDEEQEKAWRKQSRKKVMEAFEQA 393
Query: 181 EKILLPNPIQLFEDVYDTMPPRLYKQMTKHYSFITKNKEHYP 306
E+ L PNP LF DVY MP +L +Q + EHYP
Sbjct: 394 ERKLKPNPSLLFSDVYQEMPAQLRRQQESLARHLQTYGEHYP 435
>sp|P50136|ODBA_MOUSE 2-oxoisovalerate dehydrogenase alpha subunit, mitochondrial
precursor (Branched-chain alpha-keto acid dehydrogenase
E1 component alpha chain) (BCKDH E1-alpha)
Length = 442
Score = 121 bits (303), Expect = 2e-27
Identities = 55/102 (53%), Positives = 73/102 (71%)
Frame = +1
Query: 1 TSDDSLAYRSVDEISWWDKHDNPISRLKQYLLNKEWWTEKEEVSWRHEARKLVVKAIEKT 180
TSDDS AYRSVDE+++WDK D+PISRL+QYLLN+ WW E++E +WR ++RK V++A E+
Sbjct: 335 TSDDSSAYRSVDEVNYWDKQDHPISRLRQYLLNQGWWDEEQEKAWRKQSRKKVMEAFEQA 394
Query: 181 EKILLPNPIQLFEDVYDTMPPRLYKQMTKHYSFITKNKEHYP 306
E+ L PNP LF DVY MP +L +Q + EHYP
Sbjct: 395 ERKLKPNPSLLFSDVYQEMPAQLRRQQESLARHLQTYGEHYP 436
>sp|Q8HXY4|ODBA_MACFA 2-oxoisovalerate dehydrogenase alpha subunit, mitochondrial
precursor (Branched-chain alpha-keto acid dehydrogenase
E1 component alpha chain) (BCKDH E1-alpha)
Length = 445
Score = 117 bits (292), Expect = 3e-26
Identities = 54/102 (52%), Positives = 71/102 (69%)
Frame = +1
Query: 1 TSDDSLAYRSVDEISWWDKHDNPISRLKQYLLNKEWWTEKEEVSWRHEARKLVVKAIEKT 180
TSDDS AYRSVDE+++WDK D+PISRL+ YLL++ WW E++E +WR ++RK V+KA E+
Sbjct: 338 TSDDSSAYRSVDEVNYWDKQDHPISRLRHYLLSQGWWDEEQEKAWRKQSRKKVMKAFEQA 397
Query: 181 EKILLPNPIQLFEDVYDTMPPRLYKQMTKHYSFITKNKEHYP 306
E+ PNP LF DVY MP +L KQ + EHYP
Sbjct: 398 ERKPKPNPNLLFSDVYQEMPAQLRKQQESLARHLQTYGEHYP 439
>sp|P12694|ODBA_HUMAN 2-oxoisovalerate dehydrogenase alpha subunit, mitochondrial
precursor (Branched-chain alpha-keto acid dehydrogenase
E1 component alpha chain) (BCKDH E1-alpha) (BCKDE1A)
Length = 445
Score = 114 bits (285), Expect = 2e-25
Identities = 52/102 (50%), Positives = 71/102 (69%)
Frame = +1
Query: 1 TSDDSLAYRSVDEISWWDKHDNPISRLKQYLLNKEWWTEKEEVSWRHEARKLVVKAIEKT 180
TSDDS AYRSVDE+++WDK D+PISRL+ YLL++ WW E++E +WR ++R+ V++A E+
Sbjct: 338 TSDDSSAYRSVDEVNYWDKQDHPISRLRHYLLSQGWWDEEQEKAWRKQSRRKVMEAFEQA 397
Query: 181 EKILLPNPIQLFEDVYDTMPPRLYKQMTKHYSFITKNKEHYP 306
E+ PNP LF DVY MP +L KQ + EHYP
Sbjct: 398 ERKPKPNPNLLFSDVYQEMPAQLRKQQESLARHLQTYGEHYP 439
>sp|P11178|ODBA_BOVIN 2-oxoisovalerate dehydrogenase alpha subunit, mitochondrial
precursor (Branched-chain alpha-keto acid dehydrogenase
E1 component alpha chain) (BCKDH E1-alpha)
Length = 455
Score = 112 bits (281), Expect = 6e-25
Identities = 50/102 (49%), Positives = 71/102 (69%)
Frame = +1
Query: 1 TSDDSLAYRSVDEISWWDKHDNPISRLKQYLLNKEWWTEKEEVSWRHEARKLVVKAIEKT 180
TSDDS AYRSVDE+++WDK D+PISRL+ +L ++ WW +++E +WR ++RK V++A E+
Sbjct: 348 TSDDSSAYRSVDEVNYWDKQDHPISRLRHHLQSRGWWDDEQEKAWRKQSRKKVMEAFEQA 407
Query: 181 EKILLPNPIQLFEDVYDTMPPRLYKQMTKHYSFITKNKEHYP 306
E+ L PNP +F DVY MP +L KQ + EHYP
Sbjct: 408 ERKLKPNPSLIFSDVYQEMPAQLRKQQESLARHLQTYGEHYP 449
>sp|P35485|ODPA_ACHLA Pyruvate dehydrogenase E1 component, alpha subunit
Length = 345
Score = 53.1 bits (126), Expect = 5e-07
Identities = 32/96 (33%), Positives = 51/96 (53%), Gaps = 2/96 (2%)
Frame = +1
Query: 1 TSDDSLA-YRSVDEISWWDKHDNPISRLKQYLLNKEWWTEKEEVSWRHEARKLVVKAIEK 177
TSDD + YR+ +E + W K D I+R K YL+NK +W+E+E+ E + +K
Sbjct: 247 TSDDPCSIYRTKEEENEWAKKDQ-IARFKTYLINKGYWSEEEDKKLEEEVLAEINDTFKK 305
Query: 178 TEKI-LLPNPIQLFEDVYDTMPPRLYKQMTKHYSFI 282
E I++FE Y M P+L +Q +H ++
Sbjct: 306 VESYGANVELIEIFEHTYAEMTPQLKEQYEEHKKYL 341
>sp|P09060|ODBA_PSEPU 2-oxoisovalerate dehydrogenase alpha subunit (Branched-chain
alpha-keto acid dehydrogenase E1 component alpha chain)
(BCKDH E1-alpha)
Length = 410
Score = 52.8 bits (125), Expect = 7e-07
Identities = 33/92 (35%), Positives = 49/92 (53%), Gaps = 6/92 (6%)
Frame = +1
Query: 1 TSDDSLAYRSVDEISWWDKHDNPISRLKQYLLNKEWWTEKEEVSWRHEARKLVVKAIEKT 180
TSDD YR D+ S + D PI+RLKQ+L+ W+E+E + E V+ A ++
Sbjct: 314 TSDDPSKYRPADDWSHFPLGD-PIARLKQHLIKIGHWSEEEHQATTAEFEAAVIAAQKEA 372
Query: 181 EKI------LLPNPIQLFEDVYDTMPPRLYKQ 258
E+ +P+ +FEDVY MP L +Q
Sbjct: 373 EQYGTLANGHIPSAASMFEDVYKEMPDHLRRQ 404
>sp|P52901|ODPA_ARATH Pyruvate dehydrogenase E1 component alpha subunit, mitochondrial
precursor (PDHE1-A)
Length = 389
Score = 52.0 bits (123), Expect = 1e-06
Identities = 30/75 (40%), Positives = 42/75 (56%)
Frame = +1
Query: 4 SDDSLAYRSVDEISWWDKHDNPISRLKQYLLNKEWWTEKEEVSWRHEARKLVVKAIEKTE 183
SD YR+ DEIS + +PI R+K+ +L+ + TEKE E RK V AI K +
Sbjct: 294 SDPGSTYRTRDEISGVRQERDPIERIKKLVLSHDLATEKELKDMEKEIRKEVDDAIAKAK 353
Query: 184 KILLPNPIQLFEDVY 228
+P P +LF +VY
Sbjct: 354 DCPMPEPSELFTNVY 368
>sp|P52902|ODPA_PEA Pyruvate dehydrogenase E1 component alpha subunit, mitochondrial
precursor (PDHE1-A)
Length = 397
Score = 51.6 bits (122), Expect = 2e-06
Identities = 30/75 (40%), Positives = 43/75 (57%)
Frame = +1
Query: 4 SDDSLAYRSVDEISWWDKHDNPISRLKQYLLNKEWWTEKEEVSWRHEARKLVVKAIEKTE 183
SD YR+ DEIS + +PI R+++ LL+ + TEKE E RK V +AI K +
Sbjct: 302 SDPGSTYRTRDEISGVRQERDPIERVRKLLLSHDIATEKELKDTEKEVRKEVDEAIAKAK 361
Query: 184 KILLPNPIQLFEDVY 228
+P+P LF +VY
Sbjct: 362 DSPMPDPSDLFSNVY 376
>sp|P52903|ODPA_SOLTU Pyruvate dehydrogenase E1 component alpha subunit, mitochondrial
precursor (PDHE1-A)
Length = 391
Score = 47.8 bits (112), Expect = 2e-05
Identities = 27/75 (36%), Positives = 42/75 (56%)
Frame = +1
Query: 4 SDDSLAYRSVDEISWWDKHDNPISRLKQYLLNKEWWTEKEEVSWRHEARKLVVKAIEKTE 183
SD YR+ DEIS + +P+ R++ +L TE E E RK+V +AI K +
Sbjct: 296 SDPGSTYRTRDEISGVRQERDPVERIRSLILAHNIATEAELKDIEKENRKVVDEAIAKAK 355
Query: 184 KILLPNPIQLFEDVY 228
+ +P+P +LF +VY
Sbjct: 356 ESPMPDPSELFTNVY 370
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 65,041,936
Number of Sequences: 369166
Number of extensions: 1248306
Number of successful extensions: 3548
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3454
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3544
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4797864890
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)