Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_020_O18
(599 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9CWG1|GLIP1_MOUSE Glioma pathogenesis-related protein 1... 103 4e-22
sp|P16562|CRIS2_HUMAN Cysteine-rich secretory protein 2 pre... 103 4e-22
sp|Q16937|ASP_ANCCA Ancylostoma secreted protein precursor 102 6e-22
sp|Q60477|CRIS2_CAVPO Cysteine-rich secretory protein 2 pre... 101 1e-21
sp|O19010|CRIS3_HORSE Cysteine-rich secretory protein 3 pre... 100 3e-21
sp|P60623|CRVP_TRIST Cysteine-rich secretory protein precur... 97 4e-20
sp|P48060|GLIP1_HUMAN Glioma pathogenesis-related protein 1... 96 7e-20
sp|Q8JI40|CRVP_AGKHA Ablomin precursor 95 1e-19
sp|P35778|VA3_SOLIN Venom allergen 3 precursor (Venom aller... 94 2e-19
sp|P16563|CRIS2_MOUSE Cysteine-rich secretory protein 2 pre... 94 3e-19
>sp|Q9CWG1|GLIP1_MOUSE Glioma pathogenesis-related protein 1 precursor (GliPR 1)
Length = 255
Score = 103 bits (256), Expect = 4e-22
Identities = 57/162 (35%), Positives = 79/162 (48%), Gaps = 5/162 (3%)
Frame = +2
Query: 68 HNDYRRTIALGNGGQTTASNMRELSWDADLEAKAQRHAEKCTINHDTYA-DRQTSSFKMV 244
HN R ++ A NM +SWD L A+ + C H+ R +F +
Sbjct: 41 HNQLRSKVS------PPARNMLYMSWDPKLAQIAKAWTKSCEFKHNPQLHSRIHPNFTAL 94
Query: 245 GQNICLSP----DENVCQGLWYAEKDNYKFPTQQCSKVCGHYTQMVWASTYKMGCGKALC 412
G+NI L + WY E +Y F T++C VCGHYTQ+VWA +YK+GC LC
Sbjct: 95 GENIWLGSLSIFSVSSAISAWYEEIKHYDFSTRKCRHVCGHYTQVVWADSYKLGCAVQLC 154
Query: 413 NGLISLVCNYGPAGNEQGADPYTEGFPAAECPPGTTKNNGLC 538
+ +C+YGPAGN PY +G ++CP N LC
Sbjct: 155 PNGANFICDYGPAGN-YPTWPYKQGATCSDCPKDDKCLNSLC 195
>sp|P16562|CRIS2_HUMAN Cysteine-rich secretory protein 2 precursor (CRISP-2)
(Testis-specific protein TPX-1)
Length = 243
Score = 103 bits (256), Expect = 4e-22
Identities = 63/177 (35%), Positives = 91/177 (51%), Gaps = 11/177 (6%)
Frame = +2
Query: 44 LKNSLLKGHNDYRRTIALGNGGQTTASNMRELSWDADLEAKAQRHAEKCTINHDTYADRQ 223
++ ++ HN+ R+ ++ ASNM ++ W ++ AQR A KCT+ H DR+
Sbjct: 36 VQREIVNKHNELRKAVS------PPASNMLKMEWSREVTTNAQRWANKCTLQHSDPEDRK 89
Query: 224 TSSFKMVGQNICLSPDENVCQGL---WYAEKDNYKFPT--QQCSKVCGHYTQMVWASTYK 388
TS+ G+N+ +S D WY E ++ + + + V GHYTQ+VW STY+
Sbjct: 90 TST--RCGENLYMSSDPTSWSSAIQSWYDEILDFVYGVGPKSPNAVVGHYTQLVWYSTYQ 147
Query: 389 MGCGKALCNGLISL----VCNYGPAGNEQGA--DPYTEGFPAAECPPGTTKNNGLCS 541
+GCG A C SL VC Y PAGN PY +G P A CP K GLC+
Sbjct: 148 VGCGIAYCPNQDSLKYYYVCQYCPAGNNMNRKNTPYQQGTPCAGCPDDCDK--GLCT 202
>sp|Q16937|ASP_ANCCA Ancylostoma secreted protein precursor
Length = 424
Score = 102 bits (255), Expect = 6e-22
Identities = 64/195 (32%), Positives = 89/195 (45%), Gaps = 25/195 (12%)
Frame = +2
Query: 32 LDDTLKNSLLKGHNDYRRTIALG------NGGQTTASNMRELSWDADLEAKAQRHAEKCT 193
+ D+++++ L HN++R ++A G G A+ M ++ +D ++EA A RH KC
Sbjct: 233 MTDSVRDTFLSVHNEFRSSVARGLEPDALGGNAPKAAKMLKMVYDCEVEASAIRHGNKCV 292
Query: 194 INHDTYADRQTSSFKMVGQNI----CLSPDENVC----QGLWYAEKDNYKFPTQQCSKVC 349
H DR +G+NI L D+N LW+ E +
Sbjct: 293 YQHSHGEDRPG-----LGENIYKTSVLKFDKNKAAKQASQLWWNELKEFGVGPSNVLTTA 347
Query: 350 ---------GHYTQMVWASTYKMGCGKALCNGLISLVCNYGPAGNEQGADPYTEGFPAAE 502
GHYTQM W +TYK+GC CN VC YGP GN G YT G P ++
Sbjct: 348 LWNRPGMQIGHYTQMAWDTTYKLGCAVVFCNDFTFGVCQYGPGGNYMGHVIYTMGQPCSQ 407
Query: 503 CPPGTT--KNNGLCS 541
C PG T GLCS
Sbjct: 408 CSPGATCSVTEGLCS 422
Score = 47.4 bits (111), Expect = 3e-05
Identities = 47/187 (25%), Positives = 74/187 (39%), Gaps = 20/187 (10%)
Frame = +2
Query: 32 LDDTLKNSLLKGHNDYRRTIALG-----NGGQTTASNMRELSWDADLEAKAQRHAEKCTI 196
+ D + + L HN+ RR +A G +G A NM +LSWD +E + Q + C
Sbjct: 32 ITDKDRQAFLDFHNNARRRVAKGVEDSNSGKLNPAKNMYKLSWDCAMEQQLQDAIQSCP- 90
Query: 197 NHDTYADRQTSSFKMVGQNICL------SPD-----ENVCQGLWYAEKDNYKFPTQQCSK 343
+ + V QN+ PD E G W K N P + +
Sbjct: 91 -------SAFAGIQGVAQNVMSWSSSGGFPDPSVKIEQTLSGWWSGAKKNGVGPDNKYNG 143
Query: 344 V-CGHYTQMVWASTYKMGCGKALCNGLISLVCNYGPAGNEQGADPYTEG---FPAAECPP 511
++ MV++ T K+GC +C +++ C Y G + G A+C
Sbjct: 144 GGLFAFSNMVYSETTKLGCAYKVCGTKLAVSCIYNGVGYITNQPMWETGQACKTGADC-- 201
Query: 512 GTTKNNG 532
T KN+G
Sbjct: 202 STYKNSG 208
>sp|Q60477|CRIS2_CAVPO Cysteine-rich secretory protein 2 precursor (CRISP-2)
(Testis-specific protein TPX-1) (Autoantigen 1) (25 kDa
acrosomal autoantigen) (AA1)
Length = 244
Score = 101 bits (252), Expect = 1e-21
Identities = 62/187 (33%), Positives = 92/187 (49%), Gaps = 11/187 (5%)
Frame = +2
Query: 14 FLQVFCLDDTLKNSLLKGHNDYRRTIALGNGGQTTASNMRELSWDADLEAKAQRHAEKCT 193
F + ++N ++ HN R+++ ASNM ++ W + AQ+ A +CT
Sbjct: 27 FTALITTQSQVQNEIINKHNQLRKSVT------PPASNMLKMEWSREAAVNAQKWANRCT 80
Query: 194 INHDTYADRQTSSFKMVGQNICLSPDENVCQGL---WYAEKDNYKFPT--QQCSKVCGHY 358
+ H DR+TS+ G+N+ +S D + W+ E ++ F + + V GHY
Sbjct: 81 LVHSNPDDRKTST--KCGENLYMSSDPSSWSDAIQSWFDESQDFTFGVGPKSHNAVVGHY 138
Query: 359 TQMVWASTYKMGCGKALCNGLISL----VCNYGPAGNE--QGADPYTEGFPAAECPPGTT 520
TQ+VW S+Y +GCG A C SL VC Y PAGN PY +G P A CP
Sbjct: 139 TQLVWYSSYLVGCGIAYCPNQDSLKYYYVCQYCPAGNNVYTKNTPYKQGIPCASCPGHC- 197
Query: 521 KNNGLCS 541
NGLC+
Sbjct: 198 -ENGLCT 203
>sp|O19010|CRIS3_HORSE Cysteine-rich secretory protein 3 precursor (CRISP-3)
Length = 245
Score = 100 bits (249), Expect = 3e-21
Identities = 62/177 (35%), Positives = 91/177 (51%), Gaps = 11/177 (6%)
Frame = +2
Query: 44 LKNSLLKGHNDYRRTIALGNGGQTTASNMRELSWDADLEAKAQRHAEKCTINHDTYADRQ 223
++ ++ HND RRT++ ASNM ++ WD+ AQ A KC + H DR
Sbjct: 37 VQKEIVNKHNDLRRTVS------PLASNMLKMQWDSKTATNAQNWANKCLLQHSKAEDRA 90
Query: 224 TSSFKMVGQNICLSPDENVCQGL---WYAEKDNYKFPT--QQCSKVCGHYTQMVWASTYK 388
+ K G+N+ +S N W+ E ++K+ + + V GHYTQ+VW S+Y+
Sbjct: 91 VGTMKC-GENLFMSSIPNSWSDAIQNWHDEVHDFKYGVGPKTPNAVVGHYTQVVWYSSYR 149
Query: 389 MGCGKALC--NGLIS--LVCNYGPAGN--EQGADPYTEGFPAAECPPGTTKNNGLCS 541
+GCG A C G + VC Y PAGN + PY +G P A CP +NGLC+
Sbjct: 150 VGCGIAYCPKQGTLKYYYVCQYCPAGNYVNKINTPYEQGTPCARCPGNC--DNGLCT 204
>sp|P60623|CRVP_TRIST Cysteine-rich secretory protein precursor (Stecrisp)
Length = 233
Score = 96.7 bits (239), Expect = 4e-20
Identities = 61/176 (34%), Positives = 86/176 (48%), Gaps = 10/176 (5%)
Frame = +2
Query: 44 LKNSLLKGHNDYRRTIALGNGGQTTASNMRELSWDADLEAKAQRHAEKCTINHDTYADRQ 223
++N ++ HN RR++ TASNM + W + A+R A +C +H +Y R
Sbjct: 26 IQNEIVDLHNSLRRSV------NPTASNMLRMEWYPEAADNAERWAYRCIESHSSYESRV 79
Query: 224 TSSFKMVGQNICLSPDENVCQGLWYAEKDNYK-----FPTQQCSKVCGHYTQMVWASTYK 388
K G+NI +SP + +A D YK + V GHYTQ+VW +Y+
Sbjct: 80 IEGIKC-GENIYMSPYPMKWTDIIHAWHDEYKDFKYGVGADPPNAVTGHYTQIVWYKSYR 138
Query: 389 MGCGKALCNG---LISLVCNYGPAGNEQG--ADPYTEGFPAAECPPGTTKNNGLCS 541
+GC A C VC Y PAGN G A PYT G P +CP + +NGLC+
Sbjct: 139 IGCAAAYCPSSPYSYFFVCQYCPAGNFIGKTATPYTSGTPCGDCP--SDCDNGLCT 192
>sp|P48060|GLIP1_HUMAN Glioma pathogenesis-related protein 1 precursor (GliPR 1) (RTVP-1
protein)
Length = 266
Score = 95.9 bits (237), Expect = 7e-20
Identities = 60/173 (34%), Positives = 85/173 (49%), Gaps = 16/173 (9%)
Frame = +2
Query: 68 HNDYRRTIALGNGGQTTASNMRELSWDADLEAKAQRHAEKCTINHDTYAD---RQTSSFK 238
HN +R + + TAS+M ++WD L A+ A C +H+T + +F
Sbjct: 41 HNKFRSEV------KPTASDMLYMTWDPALAQIAKAWASNCQFSHNTRLKPPHKLHPNFT 94
Query: 239 MVGQNICLS--PDENVCQGL--WYAEKDNYKFPTQQCSKVCGHYTQMVWASTYKMGCGKA 406
+G+NI P +V + WY E +Y F T+ C KVCGHYTQ+VWA +YK+GC
Sbjct: 95 SLGENIWTGSVPIFSVSSAITNWYDEIQDYDFKTRICKKVCGHYTQVVWADSYKVGCAVQ 154
Query: 407 LC---NGLISL------VCNYGPAGNEQGADPYTEGFPAAECPPGTTKNNGLC 538
C +G +L +CNYGP GN PY G + CP + LC
Sbjct: 155 FCPKVSGFDALSNGAHFICNYGPGGN-YPTWPYKRGATCSACPNNDKCLDNLC 206
>sp|Q8JI40|CRVP_AGKHA Ablomin precursor
Length = 240
Score = 95.1 bits (235), Expect = 1e-19
Identities = 60/176 (34%), Positives = 89/176 (50%), Gaps = 10/176 (5%)
Frame = +2
Query: 44 LKNSLLKGHNDYRRTIALGNGGQTTASNMRELSWDADLEAKAQRHAEKCTINHDTYADRQ 223
++N ++ HN RR++ TASNM ++ W + A A+R A +C +H + R
Sbjct: 33 IQNEIVDLHNSLRRSV------NPTASNMLKMEWYPEAAANAERWAYRCIEDHSSPDSRV 86
Query: 224 TSSFKMVGQNICLSPDENVCQGL---WYAEKDNYKFP--TQQCSKVCGHYTQMVWASTYK 388
K G+NI +SP + W+ E N+K+ + V GH+TQ+VW +Y+
Sbjct: 87 LEGIKC-GENIYMSPIPMKWTDIIHIWHDEYKNFKYGIGADPPNAVSGHFTQIVWYKSYR 145
Query: 389 MGCGKALCNG---LISLVCNYGPAGNEQG--ADPYTEGFPAAECPPGTTKNNGLCS 541
GC A C VC Y PAGN +G A PYT G P +CP + +NGLC+
Sbjct: 146 AGCAAAYCPSSEYSYFYVCQYCPAGNMRGKTATPYTSGPPCGDCP--SACDNGLCT 199
>sp|P35778|VA3_SOLIN Venom allergen 3 precursor (Venom allergen III) (Allergen Sol i 3)
(Sol i III)
Length = 234
Score = 94.4 bits (233), Expect = 2e-19
Identities = 68/175 (38%), Positives = 89/175 (50%), Gaps = 28/175 (16%)
Frame = +2
Query: 38 DTLKNSLLKGHNDYRRTIALG------NGGQTTASNMRELSWDADLEAKAQRHAEKCTIN 199
D K++++ HN+ R+ +A G NG Q A M L+WD +L AQR A +CT
Sbjct: 62 DAEKDAIVNKHNELRQRVASGKEMRGTNGPQPPAVKMPNLTWDPELATIAQRWANQCTFE 121
Query: 200 HDTYADRQTSSFKMVGQNICL---------SPDENVCQGLWYAEKDNY------KFPTQQ 334
HD A R F VGQNI +P+E + LWY E ++ FP+
Sbjct: 122 HD--ACRNVERF-AVGQNIAATSSSGKNKSTPNEMIL--LWYNEVKDFDNRWISSFPSDD 176
Query: 335 --CSKVCGHYTQMVWASTYKMGCGKALCNGLIS-----LVCNYGPAGNEQGADPY 478
KV GHYTQ+VWA T K+GCG+ + + LVCNYGPAGN GA Y
Sbjct: 177 NILMKV-GHYTQIVWAKTTKIGCGRIMFKEPDNWTKHYLVCNYGPAGNVLGAPIY 230
>sp|P16563|CRIS2_MOUSE Cysteine-rich secretory protein 2 precursor (CRISP-2)
(Testis-specific protein TPX-1)
Length = 243
Score = 94.0 bits (232), Expect = 3e-19
Identities = 54/176 (30%), Positives = 91/176 (51%), Gaps = 10/176 (5%)
Frame = +2
Query: 44 LKNSLLKGHNDYRRTIALGNGGQTTASNMRELSWDADLEAKAQRHAEKCTINHDTYADRQ 223
++ ++ HN+ RR++ T S++ ++ W AQ+ A KC + H + DR+
Sbjct: 37 VQREIVNKHNELRRSV------NPTGSDILKMEWSIQATTNAQKWANKCILEHSSKDDRK 90
Query: 224 TSSFKMVGQNICLSPDENVCQGL---WYAEKDNYKFPT-QQCSKVCGHYTQMVWASTYKM 391
+ G+N+ +S D + + WY E +++ + + + GHYTQ+VW S++K+
Sbjct: 91 INI--RCGENLYMSTDPTLWSTVIQSWYNENEDFVYGVGAKPNSAVGHYTQLVWYSSFKI 148
Query: 392 GCGKALCNGLISL----VCNYGPAGNE--QGADPYTEGFPAAECPPGTTKNNGLCS 541
GCG A C +L VC+Y P GN + + PY +G P A CP NGLC+
Sbjct: 149 GCGIAYCPNQDNLKYFYVCHYCPMGNNVMKKSTPYQQGTPCASCP--NNCENGLCT 202
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 76,354,196
Number of Sequences: 369166
Number of extensions: 1737394
Number of successful extensions: 4925
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4548
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4792
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4602033670
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)