Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_020_N20
(465 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P08110|ENPL_CHICK Endoplasmin precursor (Heat shock 108 ... 94 1e-19
sp|P41148|ENPL_CANFA Endoplasmin precursor (94 kDa glucose-... 93 3e-19
sp|Q95M18|ENPL_BOVIN Endoplasmin precursor (94 kDa glucose-... 92 6e-19
sp|P08712|ENPL_MESAU Endoplasmin (94 kDa glucose-regulated ... 91 1e-18
sp|Q29092|ENPL_PIG Endoplasmin precursor (94 kDa glucose-re... 91 1e-18
sp|P14625|ENPL_HUMAN Endoplasmin precursor (94 kDa glucose-... 91 1e-18
sp|P08113|ENPL_MOUSE Endoplasmin precursor (Endoplasmic ret... 89 4e-18
sp|P27890|HSP83_LEIDO Heat shock protein 83 (HSP 83) (HSP 90) 76 3e-14
sp|O18750|ENPL_RABIT Endoplasmin (94 kDa glucose-regulated ... 76 4e-14
sp|P06660|HSP85_TRYCR Heat shock-like 85 kDa protein 75 6e-14
>sp|P08110|ENPL_CHICK Endoplasmin precursor (Heat shock 108 kDa protein) (HSP108) (HSP
108) (Transferrin-binding protein)
Length = 795
Score = 94.4 bits (233), Expect = 1e-19
Identities = 49/96 (51%), Positives = 67/96 (69%)
Frame = +2
Query: 59 SSQKKILEINPRHPLIKELKRQVEKNPSDEQARDIALTLYDTANLRSGYSLQASGGFADR 238
+SQKK EINPRHPLIK++ R+V++N D+ D+A+ L++TA LRSGY L + + DR
Sbjct: 678 ASQKKTFEINPRHPLIKDMLRRVKENEDDKTVSDLAVVLFETATLRSGYMLPDTKEYGDR 737
Query: 239 IEKILRTSLNIPHDEAVEDDEPIEEKPAEDEGEVPE 346
IE++LR SLNI D VE +EP E + A +E E E
Sbjct: 738 IERMLRLSLNIDLDAKVE-EEPEEPEDAAEEAEQDE 772
>sp|P41148|ENPL_CANFA Endoplasmin precursor (94 kDa glucose-regulated protein) (GRP94)
Length = 804
Score = 92.8 bits (229), Expect = 3e-19
Identities = 47/111 (42%), Positives = 72/111 (64%)
Frame = +2
Query: 59 SSQKKILEINPRHPLIKELKRQVEKNPSDEQARDIALTLYDTANLRSGYSLQASGGFADR 238
+SQKK EINPRHPLIK++ R+V+++ D+ D+A+ L++TA LRSGY L + + DR
Sbjct: 679 ASQKKTFEINPRHPLIKDMLRRVKEDEDDKTVSDLAVVLFETATLRSGYLLPDTKAYGDR 738
Query: 239 IEKILRTSLNIPHDEAVEDDEPIEEKPAEDEGEVPEPPKTEQPIENKEAVD 391
IE++LR SLNI + D +EE+P E+ E E + +++E +D
Sbjct: 739 IERMLRLSLNI------DPDAKVEEEPEEEPEETTEDTTEDTEQDDEEEMD 783
>sp|Q95M18|ENPL_BOVIN Endoplasmin precursor (94 kDa glucose-regulated protein) (GRP94)
Length = 804
Score = 92.0 bits (227), Expect = 6e-19
Identities = 47/112 (41%), Positives = 72/112 (64%)
Frame = +2
Query: 59 SSQKKILEINPRHPLIKELKRQVEKNPSDEQARDIALTLYDTANLRSGYSLQASGGFADR 238
+SQKK EINPRHPLI+++ R+V+++ D+ D+A+ L++TA LRSGY L + + DR
Sbjct: 679 ASQKKTFEINPRHPLIRDMLRRVKEDEDDKTVSDLAVVLFETATLRSGYLLPDTKAYGDR 738
Query: 239 IEKILRTSLNIPHDEAVEDDEPIEEKPAEDEGEVPEPPKTEQPIENKEAVDK 394
IE++LR SLNI D VE++ EE+P E + E + ++ E D+
Sbjct: 739 IERMLRLSLNIDPDAKVEEEP--EEEPEETTEDTAEDTEQDEEEEMDAGTDE 788
>sp|P08712|ENPL_MESAU Endoplasmin (94 kDa glucose-regulated protein) (GRP94)
Length = 400
Score = 91.3 bits (225), Expect = 1e-18
Identities = 45/108 (41%), Positives = 71/108 (65%)
Frame = +2
Query: 59 SSQKKILEINPRHPLIKELKRQVEKNPSDEQARDIALTLYDTANLRSGYSLQASGGFADR 238
+SQKK EINPRHPLI+++ R+V+++ D+ D+A+ L++TA LRSGY L + +ADR
Sbjct: 276 ASQKKTFEINPRHPLIRDMLRRVKEDEDDKTVLDLAVVLFETATLRSGYLLPDTKAYADR 335
Query: 239 IEKILRTSLNIPHDEAVEDDEPIEEKPAEDEGEVPEPPKTEQPIENKE 382
IE++LR SLNI + VE++ E + ++ E E + + E +E
Sbjct: 336 IERMLRLSLNIDPEAQVEEEPEEEPEDTTEDTEQDEEEEVDAGTEEEE 383
>sp|Q29092|ENPL_PIG Endoplasmin precursor (94 kDa glucose-regulated protein) (GRP94)
(GP96 homolog) (98 kDa protein kinase) (PPK 98) (ppk98)
Length = 804
Score = 91.3 bits (225), Expect = 1e-18
Identities = 47/112 (41%), Positives = 71/112 (63%)
Frame = +2
Query: 59 SSQKKILEINPRHPLIKELKRQVEKNPSDEQARDIALTLYDTANLRSGYSLQASGGFADR 238
+SQKK EINPRHPLI+++ R+V+++ D+ D+A+ L++TA LRSGY L + + DR
Sbjct: 679 ASQKKTFEINPRHPLIRDMLRRVKEDEDDKTVSDLAVVLFETATLRSGYLLPDTKAYGDR 738
Query: 239 IEKILRTSLNIPHDEAVEDDEPIEEKPAEDEGEVPEPPKTEQPIENKEAVDK 394
IE++LR SLNI D VE++ EE+P E + E + + E D+
Sbjct: 739 IERMLRLSLNIDPDAKVEEEP--EEEPEETTEDTTEDTEQDDDEEMDAGADE 788
>sp|P14625|ENPL_HUMAN Endoplasmin precursor (94 kDa glucose-regulated protein) (GRP94)
(gp96 homolog) (Tumor rejection antigen 1)
Length = 803
Score = 91.3 bits (225), Expect = 1e-18
Identities = 47/103 (45%), Positives = 70/103 (67%), Gaps = 1/103 (0%)
Frame = +2
Query: 59 SSQKKILEINPRHPLIKELKRQVEKNPSDEQARDIALTLYDTANLRSGYSLQASGGFADR 238
+SQKK EINPRHPLI+++ R+++++ D+ D+A+ L++TA LRSGY L + + DR
Sbjct: 679 ASQKKTFEINPRHPLIRDMLRRIKEDEDDKTVLDLAVVLFETATLRSGYLLPDTKAYGDR 738
Query: 239 IEKILRTSLNIPHDEAVEDD-EPIEEKPAEDEGEVPEPPKTEQ 364
IE++LR SLNI D VE++ E E+ AED E E + E+
Sbjct: 739 IERMLRLSLNIDPDAKVEEEPEEEPEETAEDTTEDTEQDEDEE 781
>sp|P08113|ENPL_MOUSE Endoplasmin precursor (Endoplasmic reticulum protein 99) (94 kDa
glucose-regulated protein) (GRP94) (ERP99) (Polymorphic
tumor rejection antigen 1) (Tumor rejection antigen gp96)
Length = 802
Score = 89.4 bits (220), Expect = 4e-18
Identities = 49/121 (40%), Positives = 75/121 (61%), Gaps = 9/121 (7%)
Frame = +2
Query: 59 SSQKKILEINPRHPLIKELKRQVEKNPSDEQARDIALTLYDTANLRSGYSLQASGGFADR 238
+SQKK EINPRHPLI+++ R+++++ D+ D+A+ L++TA LRSGY L + + DR
Sbjct: 679 ASQKKTFEINPRHPLIRDMLRRIKEDEDDKTVMDLAVVLFETATLRSGYLLPDTKAYGDR 738
Query: 239 IEKILRTSLNIPHDEAVEDDEPIE--------EKPAEDEGEVPEPPKTEQPIE-NKEAVD 391
IE++LR SLNI + VE++ E E +DEGE + E+ E KE+ +
Sbjct: 739 IERMLRLSLNIDPEAQVEEEPEEEPEDTSEDAEDSEQDEGEEMDAGTEEEEEETEKESTE 798
Query: 392 K 394
K
Sbjct: 799 K 799
>sp|P27890|HSP83_LEIDO Heat shock protein 83 (HSP 83) (HSP 90)
Length = 452
Score = 76.3 bits (186), Expect = 3e-14
Identities = 33/86 (38%), Positives = 57/86 (66%)
Frame = +2
Query: 68 KKILEINPRHPLIKELKRQVEKNPSDEQARDIALTLYDTANLRSGYSLQASGGFADRIEK 247
KK +E+NPRHP+IK L+R+VE + +D+ +D+ L+DT+ L SG+ L+ G+A RI +
Sbjct: 355 KKTMELNPRHPIIKVLRRRVEADENDKAVKDLVFLLFDTSLLTSGFQLEDPTGYAKRINR 414
Query: 248 ILRTSLNIPHDEAVEDDEPIEEKPAE 325
+++ L++ +E + +E PAE
Sbjct: 415 MIKLGLSLDEEEEAAEATVVETAPAE 440
>sp|O18750|ENPL_RABIT Endoplasmin (94 kDa glucose-regulated protein) (GRP94)
Length = 716
Score = 75.9 bits (185), Expect = 4e-14
Identities = 45/101 (44%), Positives = 64/101 (63%)
Frame = +2
Query: 62 SQKKILEINPRHPLIKELKRQVEKNPSDEQARDIALTLYDTANLRSGYSLQASGGFADRI 241
+ +K EINPRHPLI+++ R E D+ D+A+ L++TA LRSGY L + + DRI
Sbjct: 597 ASQKTFEINPRHPLIRDMLRIKE---DDKTVMDLAVVLFETAILRSGYLLPDTKAYGDRI 653
Query: 242 EKILRTSLNIPHDEAVEDDEPIEEKPAEDEGEVPEPPKTEQ 364
E+I+R SLNI D VE++ EE+P ED E E + E+
Sbjct: 654 ERIVRLSLNIDPDAKVEEEP--EEEP-EDTTEDTEQDEEEE 691
>sp|P06660|HSP85_TRYCR Heat shock-like 85 kDa protein
Length = 704
Score = 75.5 bits (184), Expect = 6e-14
Identities = 36/87 (41%), Positives = 57/87 (65%), Gaps = 1/87 (1%)
Frame = +2
Query: 68 KKILEINPRHPLIKELKRQVEKNPSDEQARDIALTLYDTANLRSGYSLQASGGFADRIEK 247
KK +EINP HP++KELKR+VE + +D+ +D+ L+DTA L SG++L +A+RI +
Sbjct: 606 KKTMEINPAHPIVKELKRRVEADENDKAVKDLVYLLFDTALLTSGFTLDDPTSYAERIHR 665
Query: 248 ILRTSLNI-PHDEAVEDDEPIEEKPAE 325
+++ L++ D E+ EP PAE
Sbjct: 666 MIKLGLSLDDEDNGNEEAEPAAAVPAE 692
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 46,934,813
Number of Sequences: 369166
Number of extensions: 817790
Number of successful extensions: 4549
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3857
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4439
length of database: 68,354,980
effective HSP length: 101
effective length of database: 49,696,745
effective search space used: 2633927485
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)