Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_020_M18
(834 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9UHV9|PFD2_HUMAN Prefoldin subunit 2 98 2e-20
sp|O70591|PFD2_MOUSE Prefoldin subunit 2 98 3e-20
sp|Q9VTE5|PFD2_DROME Probable prefoldin subunit 2 94 4e-19
sp|Q9LJ98|PFD2_ARATH Probable prefoldin subunit 2 76 1e-13
sp|Q9N5M2|PFD2_CAEEL Probable prefoldin subunit 2 63 9e-10
sp|Q9UTC9|PFD2_SCHPO Probable prefoldin subunit 2 54 4e-07
sp|P40005|PFD2_YEAST Prefoldin subunit 2 54 4e-07
sp|Q8TYC7|PFDB_METKA Prefoldin beta subunit (GimC beta subu... 48 3e-05
sp|Q9VW56|PFD6_DROME Probable prefoldin subunit 6 40 0.010
sp|Q00816|HEX2_YEAST Protein HEX2 (Protein SRN1) (Protein R... 35 0.20
>sp|Q9UHV9|PFD2_HUMAN Prefoldin subunit 2
Length = 154
Score = 98.2 bits (243), Expect = 2e-20
Identities = 46/98 (46%), Positives = 65/98 (66%)
Frame = +3
Query: 534 KNAKSQQEIFTELQNMLMEQKAMAGKLSELEMELREHGLVIKVLSEANSDRKCFRTLGGV 713
K A S +++ + EQ+ +A K +ELEMEL EH LVI L E + RKC+R +GGV
Sbjct: 18 KGAVSAEQVIAGFNRLRQEQRGLASKAAELEMELNEHSLVIDTLKEVDETRKCYRMVGGV 77
Query: 714 LVEQTVKEVLPCLQENEEQLKKMIEVINNNCNQKTKDI 827
LVE+TVKEVLP L+ N+EQ++K+IE + K K++
Sbjct: 78 LVERTVKEVLPALENNKEQIQKIIETLTQQLQAKGKEL 115
>sp|O70591|PFD2_MOUSE Prefoldin subunit 2
Length = 154
Score = 97.8 bits (242), Expect = 3e-20
Identities = 46/98 (46%), Positives = 66/98 (67%)
Frame = +3
Query: 534 KNAKSQQEIFTELQNMLMEQKAMAGKLSELEMELREHGLVIKVLSEANSDRKCFRTLGGV 713
K A S +++ + EQ+ +A K +ELEMEL EH LVI L E + RKC+R +GGV
Sbjct: 18 KGAVSAEQVIAGFNRLRQEQRGLASKAAELEMELNEHSLVIDTLKEVDETRKCYRMVGGV 77
Query: 714 LVEQTVKEVLPCLQENEEQLKKMIEVINNNCNQKTKDI 827
LVE+TVKEVLP L+ N+EQ++K+IE ++ K K++
Sbjct: 78 LVERTVKEVLPALEGNKEQIQKIIETLSQQLQAKGKEL 115
>sp|Q9VTE5|PFD2_DROME Probable prefoldin subunit 2
Length = 143
Score = 94.4 bits (233), Expect = 4e-19
Identities = 47/104 (45%), Positives = 71/104 (68%)
Frame = +3
Query: 516 MSKKSDKNAKSQQEIFTELQNMLMEQKAMAGKLSELEMELREHGLVIKVLSEANSDRKCF 695
MS +S K A SQ+ I + Q + EQ+ + L+ LEM+LREH VI+ L A+ +RKCF
Sbjct: 1 MSTESAKPALSQEAIVAQFQQLRNEQRNLVNSLNTLEMDLREHKTVIETLEAADPERKCF 60
Query: 696 RTLGGVLVEQTVKEVLPCLQENEEQLKKMIEVINNNCNQKTKDI 827
R +GGVL E+TVKEVLP L EN++ + K I+++ N+ ++K ++
Sbjct: 61 RQIGGVLCERTVKEVLPQLVENKDFIAKTIQMVTNDLSKKGSEL 104
>sp|Q9LJ98|PFD2_ARATH Probable prefoldin subunit 2
Length = 148
Score = 75.9 bits (185), Expect = 1e-13
Identities = 33/94 (35%), Positives = 58/94 (61%)
Frame = +3
Query: 546 SQQEIFTELQNMLMEQKAMAGKLSELEMELREHGLVIKVLSEANSDRKCFRTLGGVLVEQ 725
++Q + + E + +++LEM++ EH LVI + + RKCFR +GGVLVE+
Sbjct: 15 NEQAVLNMYEGKRSELSQIYSNITDLEMQVSEHSLVINAIQPLDQSRKCFRMIGGVLVER 74
Query: 726 TVKEVLPCLQENEEQLKKMIEVINNNCNQKTKDI 827
T+KEVLP +Q N++ L++++ + +K KD+
Sbjct: 75 TIKEVLPAVQRNKDGLEEVVRKLYETLEKKKKDL 108
>sp|Q9N5M2|PFD2_CAEEL Probable prefoldin subunit 2
Length = 141
Score = 63.2 bits (152), Expect = 9e-10
Identities = 30/95 (31%), Positives = 59/95 (62%)
Frame = +3
Query: 543 KSQQEIFTELQNMLMEQKAMAGKLSELEMELREHGLVIKVLSEANSDRKCFRTLGGVLVE 722
+ Q+++ + + + +Q+ +A +++ +E E RE G V++V+ + D+KCFR + LVE
Sbjct: 15 EEQRKVVEKFKALRDQQQDIAAEVTRIEEERREFGRVLEVIKDLEPDQKCFRLISDTLVE 74
Query: 723 QTVKEVLPCLQENEEQLKKMIEVINNNCNQKTKDI 827
TVK+V+P LQ N L + + +N+ +K K++
Sbjct: 75 YTVKDVIPDLQNNIANLTIVSKQLNDQLVEKGKEL 109
>sp|Q9UTC9|PFD2_SCHPO Probable prefoldin subunit 2
Length = 114
Score = 54.3 bits (129), Expect = 4e-07
Identities = 27/76 (35%), Positives = 48/76 (63%)
Frame = +3
Query: 549 QQEIFTELQNMLMEQKAMAGKLSELEMELREHGLVIKVLSEANSDRKCFRTLGGVLVEQT 728
QQ + T+ + + +A K+ +LE + EH LV+ L+ +++R+CFR + GVLVE+T
Sbjct: 8 QQILQTQYNSYKSRLQQIAQKIVDLETDADEHKLVMDTLNSMDNNRRCFRMIHGVLVERT 67
Query: 729 VKEVLPCLQENEEQLK 776
V V+P L+ +E ++
Sbjct: 68 VGTVVPILKTTQEGIQ 83
>sp|P40005|PFD2_YEAST Prefoldin subunit 2
Length = 123
Score = 54.3 bits (129), Expect = 4e-07
Identities = 31/91 (34%), Positives = 48/91 (52%)
Frame = +3
Query: 552 QEIFTELQNMLMEQKAMAGKLSELEMELREHGLVIKVLSEANSDRKCFRTLGGVLVEQTV 731
Q + E + +L E + K+ EL + EH +VIK L +A RKC+R +GG LVE V
Sbjct: 21 QAKYNEYKQILEELQT---KIIELGHDKDEHTIVIKTLKDAEPTRKCYRMIGGALVESDV 77
Query: 732 KEVLPCLQENEEQLKKMIEVINNNCNQKTKD 824
+ LP L+ +E ++ I + Q K+
Sbjct: 78 QTSLPILETKKENIEGTISKMKETLIQTAKE 108
>sp|Q8TYC7|PFDB_METKA Prefoldin beta subunit (GimC beta subunit)
Length = 120
Score = 48.1 bits (113), Expect = 3e-05
Identities = 23/85 (27%), Positives = 49/85 (57%)
Frame = +3
Query: 540 AKSQQEIFTELQNMLMEQKAMAGKLSELEMELREHGLVIKVLSEANSDRKCFRTLGGVLV 719
A++ ++ +LQ + + ++ + +LE++LRE +K L E D K ++T+GG+L+
Sbjct: 2 AQNVEQQVAQLQQLQQQLSSIVAQKQQLELQLREIERALKELDEIEEDTKVYKTVGGLLI 61
Query: 720 EQTVKEVLPCLQENEEQLKKMIEVI 794
E EV L++ +E L+ ++ +
Sbjct: 62 EADRDEVKEELEDRKETLELRVKTL 86
>sp|Q9VW56|PFD6_DROME Probable prefoldin subunit 6
Length = 125
Score = 39.7 bits (91), Expect = 0.010
Identities = 29/101 (28%), Positives = 44/101 (43%)
Frame = +3
Query: 516 MSKKSDKNAKSQQEIFTELQNMLMEQKAMAGKLSELEMELREHGLVIKVLSEANSDRKCF 695
M KKS K Q QN+ M + + LE +L E+ V+ L+ D K +
Sbjct: 1 MDKKSAALYKKMQAEIESYQNLQKSCLKMVKQRAVLESQLNENKCVLDELNLLGPDNKVY 60
Query: 696 RTLGGVLVEQTVKEVLPCLQENEEQLKKMIEVINNNCNQKT 818
+ G VLV+Q L+E+ + + K IE I+ T
Sbjct: 61 KLFGPVLVKQE-------LEESRQNVGKRIEYISKELKSST 94
>sp|Q00816|HEX2_YEAST Protein HEX2 (Protein SRN1) (Protein REG1)
Length = 1014
Score = 35.4 bits (80), Expect = 0.20
Identities = 25/78 (32%), Positives = 44/78 (56%), Gaps = 1/78 (1%)
Frame = -1
Query: 330 KSTYQEIRDQISIENIKWIGQNSIGLLSNIKESLSAKYKVEVGQNPSVEEVNQND-EDST 154
KS + + +D +I N +G+NS G +E L K + V N S + N++ E+ST
Sbjct: 351 KSQHSDKKDNSTIPNP--VGENSNGGGDKGEEDLHLKSALHVQNNRSTAQSNKSILENST 408
Query: 153 HDEEANLRDYLSTNDDHQ 100
+D +ANL L++ D+++
Sbjct: 409 NDRKANLDQNLNSPDNNR 426
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 88,466,127
Number of Sequences: 369166
Number of extensions: 1728601
Number of successful extensions: 5850
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5563
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5838
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8100769320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)