Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_020_L17
(925 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P41892|CDC7_SCHPO Cell division control protein 7 68 3e-11
sp|P27636|CDC15_YEAST Cell division control protein 15 64 6e-10
sp|Q13233|M3K1_HUMAN Mitogen-activated protein kinase kinas... 63 1e-09
sp|Q01389|BCK1_YEAST Serine/threonine-protein kinase BCK1/S... 62 2e-09
sp|P53349|M3K1_MOUSE Mitogen-activated protein kinase kinas... 62 2e-09
sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinas... 62 2e-09
sp|Q03306|PKH3_YEAST Serine/threonine-protein kinase PKH3 (... 61 5e-09
sp|Q62925|M3K1_RAT Mitogen-activated protein kinase kinase ... 61 5e-09
sp|P38991|IPL1_YEAST Spindle assembly checkpoint kinase (Au... 60 7e-09
sp|Q8ICR0|CDPK2_PLAF7 Calcium-dependent protein kinase 2 (P... 60 9e-09
>sp|P41892|CDC7_SCHPO Cell division control protein 7
Length = 1062
Score = 68.2 bits (165), Expect = 3e-11
Identities = 39/116 (33%), Positives = 63/116 (54%)
Frame = +3
Query: 576 LGHGPFGSVYRCSKFGSKDMVVLKKYKNPNDKDEELKKLMVEFSIYEKLGDHPRILKCNG 755
LG G FG+VYR + + V +KK K +L + +E + + L DHP I+K G
Sbjct: 15 LGKGAFGAVYRGLNIKNGETVAVKKVKLSKMLKSDLSVIKMEIDLLKNL-DHPNIVKYRG 73
Query: 756 FFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILH 923
+++ + I L+Y NGSLRS G E + ++ Q+L+ L +LH+Q ++H
Sbjct: 74 SYQTNDSLCIILEYCENGSLRSICKNFGKIPENLVALYTFQVLQGLLYLHNQGVIH 129
>sp|P27636|CDC15_YEAST Cell division control protein 15
Length = 974
Score = 63.9 bits (154), Expect = 6e-10
Identities = 40/123 (32%), Positives = 63/123 (51%), Gaps = 1/123 (0%)
Frame = +3
Query: 558 YIKHEELGHGPFGSVYRCSKFGSKDMVVLKKYKNPNDKDEELKKLMVEFSIYEKLGDHPR 737
Y + +G G +G VY+ + +V +K+ ND EEL +M E S+ + L +H
Sbjct: 25 YHLKQVIGRGSYGVVYKAINKHTDQVVAIKEVVYEND--EELNDIMAEISLLKNL-NHNN 81
Query: 738 ILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNG-AQKEPSIIRFSKQILEALDFLHSQN 914
I+K +GF R YI L+Y ANGSLR + ++ E + Q L L +LH +
Sbjct: 82 IVKYHGFIRKSYELYILLEYCANGSLRRLISRSSTGLSENESKTYVTQTLLGLKYLHGEG 141
Query: 915 ILH 923
++H
Sbjct: 142 VIH 144
>sp|Q13233|M3K1_HUMAN Mitogen-activated protein kinase kinase kinase 1 (MAPK/ERK kinase
kinase 1) (MEK kinase 1) (MEKK 1)
Length = 1495
Score = 63.2 bits (152), Expect = 1e-09
Identities = 41/163 (25%), Positives = 80/163 (49%), Gaps = 4/163 (2%)
Frame = +3
Query: 447 QDENKEKQVKWDEDYEQLISGGKSISLFYLPTPDFDDYIKHEELGHGPFGSVYRCSKFGS 626
Q EN E + +D + + G P + +++K +++G G F S Y+ G+
Sbjct: 1192 QVENGEDIIIIQQDTPETLPGHTKAKQ---PYREDTEWLKGQQIGLGAFSSCYQAQDVGT 1248
Query: 627 KDMVVLKKY----KNPNDKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRSVKASYIKLD 794
++ +K+ ++++E ++ L E + L +HP I++ G + ++
Sbjct: 1249 GTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHL-NHPNIIRMLGATCEKSNYNLFIE 1307
Query: 795 YMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILH 923
+MA GS+ L K GA KE +I +++Q+L L +LH I+H
Sbjct: 1308 WMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIH 1350
>sp|Q01389|BCK1_YEAST Serine/threonine-protein kinase BCK1/SLK1/SSP31
Length = 1478
Score = 62.4 bits (150), Expect = 2e-09
Identities = 40/128 (31%), Positives = 66/128 (51%), Gaps = 6/128 (4%)
Frame = +3
Query: 558 YIKHEELGHGPFGSVYRCSKFGSKDMVVLKKYKNPNDKDEE------LKKLMVEFSIYEK 719
++K E +G G FG+VY C + +M+ +K+ + P + ++ L E S +
Sbjct: 1175 WMKGEMIGKGSFGAVYLCLNVTTGEMMAVKQVEVPKYSSQNEAILSTVEALRSEVSTLKD 1234
Query: 720 LGDHPRILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDF 899
L DH I++ GF + L+Y+A GS+ S + G EP I + Q+L+ L +
Sbjct: 1235 L-DHLNIVQYLGFENKNNIYSLFLEYVAGGSVGSLIRMYGRFDEPLIKHLTTQVLKGLAY 1293
Query: 900 LHSQNILH 923
LHS+ ILH
Sbjct: 1294 LHSKGILH 1301
>sp|P53349|M3K1_MOUSE Mitogen-activated protein kinase kinase kinase 1 (MAPK/ERK kinase
kinase 1) (MEK kinase 1) (MEKK 1)
Length = 1493
Score = 62.0 bits (149), Expect = 2e-09
Identities = 41/163 (25%), Positives = 80/163 (49%), Gaps = 4/163 (2%)
Frame = +3
Query: 447 QDENKEKQVKWDEDYEQLISGGKSISLFYLPTPDFDDYIKHEELGHGPFGSVYRCSKFGS 626
Q EN E + +D + + G P + +++K +++G G F S Y+ G+
Sbjct: 1190 QVENGEDIIIIQQDTPETLPGHTKAKQ---PYREDAEWLKGQQIGLGAFSSCYQAQDVGT 1246
Query: 627 KDMVVLKKY----KNPNDKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRSVKASYIKLD 794
++ +K+ ++++E ++ L E + L +HP I++ G + ++
Sbjct: 1247 GTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMGHL-NHPNIIRMLGATCEKSNYNLFIE 1305
Query: 795 YMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILH 923
+MA GS+ L K GA KE +I +++Q+L L +LH I+H
Sbjct: 1306 WMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIH 1348
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
NPK1-related protein kinase 3)
Length = 651
Score = 62.0 bits (149), Expect = 2e-09
Identities = 39/126 (30%), Positives = 68/126 (53%), Gaps = 6/126 (4%)
Frame = +3
Query: 564 KHEELGHGPFGSVYRCSKFGSKDMVVLKKYK-NPNDKDEE-----LKKLMVEFSIYEKLG 725
K E +G G FG VY S +++ +K+ P+ +E +++L E + + L
Sbjct: 70 KGELIGCGAFGRVYMGMNLDSGELLAIKQVLIAPSSASKEKTQGHIRELEEEVQLLKNLS 129
Query: 726 DHPRILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLH 905
HP I++ G R + I ++++ GS+ S L K G+ EP II ++KQ+L L++LH
Sbjct: 130 -HPNIVRYLGTVRESDSLNILMEFVPGGSISSLLEKFGSFPEPVIIMYTKQLLLGLEYLH 188
Query: 906 SQNILH 923
+ I+H
Sbjct: 189 NNGIMH 194
>sp|Q03306|PKH3_YEAST Serine/threonine-protein kinase PKH3 (Pkb-activating kinase homolog
3)
Length = 898
Score = 60.8 bits (146), Expect = 5e-09
Identities = 39/127 (30%), Positives = 63/127 (49%), Gaps = 4/127 (3%)
Frame = +3
Query: 555 DYIKHEELGHGPFGSVYRCSKFGSKDMVVLKKY--KNPNDKDEELKKLMVEFSIYEKLGD 728
D+I EELGHG + +V++ S + + K K K+ ++K + +E + L
Sbjct: 10 DFIFKEELGHGSYSTVFKALDKKSPNKIYAIKVCSKKHIIKEAKVKYVTIEKNTMNLLAQ 69
Query: 729 --HPRILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFL 902
H I+K F + Y LD+ G L S LHK G + F+ Q+++AL+F+
Sbjct: 70 KHHAGIIKLYYTFHDEENLYFVLDFAPGGELLSLLHKMGTFNDIWTRHFTAQLIDALEFI 129
Query: 903 HSQNILH 923
HS I+H
Sbjct: 130 HSHGIIH 136
>sp|Q62925|M3K1_RAT Mitogen-activated protein kinase kinase kinase 1 (MAPK/ERK kinase
kinase 1) (MEK kinase 1) (MEKK 1)
Length = 1493
Score = 60.8 bits (146), Expect = 5e-09
Identities = 40/163 (24%), Positives = 80/163 (49%), Gaps = 4/163 (2%)
Frame = +3
Query: 447 QDENKEKQVKWDEDYEQLISGGKSISLFYLPTPDFDDYIKHEELGHGPFGSVYRCSKFGS 626
Q EN E + +D + + G + P + +++K +++G G F S Y+ G+
Sbjct: 1190 QVENGEDIIIIQQDTPETLPGHTKANE---PYREDTEWLKGQQIGLGAFSSCYQAQDVGT 1246
Query: 627 KDMVVLKKY----KNPNDKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRSVKASYIKLD 794
++ +K+ ++++E ++ L E + L +HP I++ G + ++
Sbjct: 1247 GTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHL-NHPNIIRMLGATCEKSNYNLFIE 1305
Query: 795 YMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILH 923
+MA S+ L K GA KE +I +++Q+L L +LH I+H
Sbjct: 1306 WMAGASVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIH 1348
>sp|P38991|IPL1_YEAST Spindle assembly checkpoint kinase (Aurora kinase)
(Increase-in-ploidy protein 1)
Length = 367
Score = 60.5 bits (145), Expect = 7e-09
Identities = 38/126 (30%), Positives = 60/126 (47%), Gaps = 2/126 (1%)
Frame = +3
Query: 552 DDYIKHEELGHGPFGSVYRCSKFGSKDMVVLKKYKNPND--KDEELKKLMVEFSIYEKLG 725
DD+ ++LG G FG VY C + S + K + K K+ E I L
Sbjct: 102 DDFELGKKLGKGKFGKVY-CVRHRSTGYICALKVMEKEEIIKYNLQKQFRREVEIQTSL- 159
Query: 726 DHPRILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLH 905
+HP + K G+F K Y+ ++Y+ NG + L +G + + QI ALD++H
Sbjct: 160 NHPNLTKSYGYFHDEKRVYLLMEYLVNGEMYKLLRLHGPFNDILASDYIYQIANALDYMH 219
Query: 906 SQNILH 923
+NI+H
Sbjct: 220 KKNIIH 225
>sp|Q8ICR0|CDPK2_PLAF7 Calcium-dependent protein kinase 2 (PfCDPK2)
Length = 509
Score = 60.1 bits (144), Expect = 9e-09
Identities = 37/124 (29%), Positives = 63/124 (50%)
Frame = +3
Query: 552 DDYIKHEELGHGPFGSVYRCSKFGSKDMVVLKKYKNPNDKDEELKKLMVEFSIYEKLGDH 731
D YI E+LG G +G VY+ + + +K+ K D+ + + + E I +KL DH
Sbjct: 70 DKYIIDEKLGQGTYGCVYKGIDKVTNQLYAIKEEKK--DRLKNINRFFQEIEIMKKL-DH 126
Query: 732 PRILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQ 911
P I+K + + Y+ ++ + L + +NG+ E + KQI A+ +LHS
Sbjct: 127 PNIVKLYETYENDNYIYLIMELCSGRELFDSIIENGSFTEKNAATIMKQIFSAIFYLHSL 186
Query: 912 NILH 923
NI+H
Sbjct: 187 NIVH 190
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 95,290,681
Number of Sequences: 369166
Number of extensions: 1922906
Number of successful extensions: 6642
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6063
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6318
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9462723610
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)