Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_020_L08
(818 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q11067|PDIA6_CAEEL Probable protein disulfide-isomerase ... 270 3e-72
sp|Q922R8|PDIA6_MOUSE Protein disulfide-isomerase A6 precur... 264 3e-70
sp|Q63081|PDIA6_RAT Protein disulfide-isomerase A6 precurso... 261 1e-69
sp|Q15084|PDIA6_HUMAN Protein disulfide-isomerase A6 precur... 257 2e-68
sp|Q5R6T1|PDIA6_PONPY Protein disulfide-isomerase A6 precursor 254 2e-67
sp|P38660|PDIA6_MESAU Protein disulfide-isomerase A6 precur... 253 6e-67
sp|Q17770|PDI2_CAEEL Protein disulfide-isomerase 2 precurso... 67 6e-11
sp|P54399|PDI_DROME Protein disulfide-isomerase precursor (... 65 3e-10
sp|P21195|PDIA1_RABIT Protein disulfide-isomerase precursor... 59 2e-08
sp|P09103|PDIA1_MOUSE Protein disulfide-isomerase precursor... 58 4e-08
>sp|Q11067|PDIA6_CAEEL Probable protein disulfide-isomerase A6 precursor
Length = 440
Score = 270 bits (690), Expect = 3e-72
Identities = 120/230 (52%), Positives = 168/230 (73%)
Frame = +3
Query: 3 GKVKLGALDATVHNAVASRYGVQGYPTIKFFPAGVKTGEAQEYDGGRSSSDIVRWASDKV 182
GKV+LGALDATVH VA+++ ++G+PTIK+F G +AQ+YDGGR SSDIV WAS +
Sbjct: 213 GKVRLGALDATVHTVVANKFAIRGFPTIKYFAPGSDVSDAQDYDGGRQSSDIVAWASARA 272
Query: 183 VEQLPAPELKEITNQDVLKSTCEDHQLCVIAVLPNILDCQSACRNSYLDTIRVSSDKYKA 362
E +PAPE+ E NQ V++ C++ QLC+ A LP+ILDCQS CRN+YL ++ S+K+K
Sbjct: 273 QENMPAPEVFEGINQQVVEDACKEKQLCIFAFLPHILDCQSECRNNYLAMLKEQSEKFKK 332
Query: 363 NRWGWCWTEAMKLPAIEKAFGIGGFGYPALVVVNVRKMKYATLTGPFSQTGIQDFVRDVS 542
N WGW W E PA+E++F +GGFGYPA+ +N RK KYA L G F + GI +F+RD+S
Sbjct: 333 NLWGWIWVEGAAQPALEESFEVGGFGYPAMTALNFRKNKYAVLKGSFGKDGIHEFLRDLS 392
Query: 543 LGRGSTAAIVGEGLPAITPVDPWDGKDGEMPKEEEIDLSELGIEEPKSEL 692
G+G T+++ G+G P I + WDGKDG +P E++IDLS++ ++ K+EL
Sbjct: 393 YGKGRTSSLRGDGFPKIQKTEKWDGKDGALPAEDDIDLSDIDLD--KTEL 440
Score = 44.3 bits (103), Expect = 4e-04
Identities = 22/52 (42%), Positives = 32/52 (61%)
Frame = +3
Query: 3 GKVKLGALDATVHNAVASRYGVQGYPTIKFFPAGVKTGEAQEYDGGRSSSDI 158
G K+GA+D T H +V Y VQG+PT+K F A K + +Y+G R++ I
Sbjct: 73 GVAKVGAVDMTQHQSVGGPYNVQGFPTLKIFGADKK--KPTDYNGQRTAQAI 122
>sp|Q922R8|PDIA6_MOUSE Protein disulfide-isomerase A6 precursor (Thioredoxin
domain-containing protein 7)
Length = 440
Score = 264 bits (674), Expect = 3e-70
Identities = 128/231 (55%), Positives = 165/231 (71%), Gaps = 1/231 (0%)
Frame = +3
Query: 3 GKVKLGALDATVHNAVASRYGVQGYPTIKFFPAGVKTGEAQEYDGGRSSSDIVRWASDKV 182
GKVKL A+DATV+ +ASRYG++G+PTIK F G +YDGGR+ SDIV A D
Sbjct: 213 GKVKLAAVDATVNQVLASRYGIKGFPTIKIFQKGESP---VDYDGGRTRSDIVSRALDLF 269
Query: 183 VEQLPAPELKEITNQDVLKSTCEDHQLCVIAVLPNILDCQSACRNSYLDTIRVSSDKYKA 362
+ P PEL EI N+D+ K TCE+HQLCV+AVLP+ILD +A RNSYL+ + +DKYK
Sbjct: 270 SDNAPPPELLEIINEDIAKKTCEEHQLCVVAVLPHILDTGAAGRNSYLEVLLKLADKYKK 329
Query: 363 NRWGWCWTEAMKLPAIEKAFGIGGFGYPALVVVNVRKMKYATLTGPFSQTGIQDFVRDVS 542
WGW WTEA +E A GIGGFGYPA+ +N RKMK+A L G FS+ GI +F+R++S
Sbjct: 330 KMWGWLWTEAGAQYELENALGIGGFGYPAMAAINARKMKFALLKGSFSEQGINEFLRELS 389
Query: 543 LGRGSTAAIVGEGLPAITPVDPWDGKDGEMPKEEEIDLSELGIEE-PKSEL 692
GRGSTA + G P ITP +PWDGKDGE+P E++IDLS++ +++ K EL
Sbjct: 390 FGRGSTAPVGGGSFPTITprePWDGKDGELPVEDDIDLSDVELDDLEKDEL 440
Score = 50.4 bits (119), Expect = 6e-06
Identities = 23/51 (45%), Positives = 34/51 (66%)
Frame = +3
Query: 9 VKLGALDATVHNAVASRYGVQGYPTIKFFPAGVKTGEAQEYDGGRSSSDIV 161
VK+GA++A H ++ +YGVQG+PTIK F G + ++Y GGR+ IV
Sbjct: 76 VKVGAVNADKHQSLGGQYGVQGFPTIKIF--GANKNKPEDYQGGRTGEAIV 124
>sp|Q63081|PDIA6_RAT Protein disulfide-isomerase A6 precursor (Protein disulfide
isomerase P5) (Calcium-binding protein 1) (CaBP1)
(Thioredoxin domain-containing protein 7)
Length = 440
Score = 261 bits (668), Expect = 1e-69
Identities = 127/231 (54%), Positives = 164/231 (70%), Gaps = 1/231 (0%)
Frame = +3
Query: 3 GKVKLGALDATVHNAVASRYGVQGYPTIKFFPAGVKTGEAQEYDGGRSSSDIVRWASDKV 182
GKVKL A+DATV+ +ASRYG++G+PTIK F G +YDGGR+ SDIV A D
Sbjct: 213 GKVKLAAVDATVNQVLASRYGIKGFPTIKIFQKGESP---VDYDGGRTRSDIVSRALDLF 269
Query: 183 VEQLPAPELKEITNQDVLKSTCEDHQLCVIAVLPNILDCQSACRNSYLDTIRVSSDKYKA 362
+ P PEL EI N+D+ K TCE+HQLCV+AVLP+ILD + RNSYL+ + +DKYK
Sbjct: 270 SDNAPPPELLEIINEDIAKKTCEEHQLCVVAVLPHILDTGATGRNSYLEVLLKLADKYKK 329
Query: 363 NRWGWCWTEAMKLPAIEKAFGIGGFGYPALVVVNVRKMKYATLTGPFSQTGIQDFVRDVS 542
WGW WTEA +E A GIGGFGYPA+ +N RKMK+A L G FS+ GI +F+R++S
Sbjct: 330 KMWGWLWTEAGAQYELENALGIGGFGYPAMAAINARKMKFALLKGSFSEQGINEFLRELS 389
Query: 543 LGRGSTAAIVGEGLPAITPVDPWDGKDGEMPKEEEIDLSELGIEE-PKSEL 692
GRGSTA + G P ITP +PWDGKDGE+P E++IDLS++ +++ K EL
Sbjct: 390 FGRGSTAPVGGGSFPNITprePWDGKDGELPVEDDIDLSDVELDDLEKDEL 440
Score = 50.4 bits (119), Expect = 6e-06
Identities = 23/51 (45%), Positives = 34/51 (66%)
Frame = +3
Query: 9 VKLGALDATVHNAVASRYGVQGYPTIKFFPAGVKTGEAQEYDGGRSSSDIV 161
VK+GA++A H ++ +YGVQG+PTIK F G + ++Y GGR+ IV
Sbjct: 76 VKVGAVNADKHQSLGGQYGVQGFPTIKIF--GANKNKPEDYQGGRTGEAIV 124
>sp|Q15084|PDIA6_HUMAN Protein disulfide-isomerase A6 precursor (Protein disulfide
isomerase P5) (Thioredoxin domain-containing protein 7)
Length = 440
Score = 257 bits (657), Expect = 2e-68
Identities = 122/225 (54%), Positives = 160/225 (71%)
Frame = +3
Query: 3 GKVKLGALDATVHNAVASRYGVQGYPTIKFFPAGVKTGEAQEYDGGRSSSDIVRWASDKV 182
GKVKL A+DATV+ +ASRYG++G+PTIK F G +YDGGR+ SDIV A D
Sbjct: 213 GKVKLAAVDATVNQVLASRYGIRGFPTIKIFQKGESP---VDYDGGRTRSDIVSRALDLF 269
Query: 183 VEQLPAPELKEITNQDVLKSTCEDHQLCVIAVLPNILDCQSACRNSYLDTIRVSSDKYKA 362
+ P PEL EI N+D+ K TCE+HQLCV+AVLP+ILD +A RNSYL+ + +DKYK
Sbjct: 270 SDNAPPPELLEIINEDIAKRTCEEHQLCVVAVLPHILDTGAAGRNSYLEVLLKLADKYKK 329
Query: 363 NRWGWCWTEAMKLPAIEKAFGIGGFGYPALVVVNVRKMKYATLTGPFSQTGIQDFVRDVS 542
WGW WTEA +E A GIGGFGYPA+ +N RKMK+A L G FS+ GI +F+R++S
Sbjct: 330 KMWGWLWTEAGAQSELETALGIGGFGYPAMAAINARKMKFALLKGSFSEQGINEFLRELS 389
Query: 543 LGRGSTAAIVGEGLPAITPVDPWDGKDGEMPKEEEIDLSELGIEE 677
GRGSTA + G P I +PWDG+DGE+P E++IDLS++ +++
Sbjct: 390 FGRGSTAPVGGGAFPTIVEREPWDGRDGELPVEDDIDLSDVELDD 434
Score = 51.6 bits (122), Expect = 3e-06
Identities = 24/51 (47%), Positives = 34/51 (66%)
Frame = +3
Query: 9 VKLGALDATVHNAVASRYGVQGYPTIKFFPAGVKTGEAQEYDGGRSSSDIV 161
VK+GA+DA H+++ +YGVQG+PTIK F G ++Y GGR+ IV
Sbjct: 76 VKVGAVDADKHHSLGGQYGVQGFPTIKIF--GSNKNRPEDYQGGRTGEAIV 124
>sp|Q5R6T1|PDIA6_PONPY Protein disulfide-isomerase A6 precursor
Length = 440
Score = 254 bits (649), Expect = 2e-67
Identities = 120/225 (53%), Positives = 160/225 (71%)
Frame = +3
Query: 3 GKVKLGALDATVHNAVASRYGVQGYPTIKFFPAGVKTGEAQEYDGGRSSSDIVRWASDKV 182
GKVKL A+DATV+ +ASRYG++G+PTIK F G +YDGGR+ SDIV A D
Sbjct: 213 GKVKLAAVDATVNQVLASRYGIRGFPTIKIFQKGESP---VDYDGGRTRSDIVSRALDLF 269
Query: 183 VEQLPAPELKEITNQDVLKSTCEDHQLCVIAVLPNILDCQSACRNSYLDTIRVSSDKYKA 362
+ P PEL EI ++D+ K TCE+HQLCV++VLP+ILD +A RNSYL+ + +DKYK
Sbjct: 270 SDNAPPPELLEIISEDIAKRTCEEHQLCVVSVLPHILDTGAAGRNSYLEVLLKLADKYKK 329
Query: 363 NRWGWCWTEAMKLPAIEKAFGIGGFGYPALVVVNVRKMKYATLTGPFSQTGIQDFVRDVS 542
WGW WTEA +E A GIGGFGYPA+ +N RKMK+A L G FS+ GI +F+R++S
Sbjct: 330 KMWGWLWTEAGAQSELETALGIGGFGYPAMAAINARKMKFALLKGSFSEQGINEFLRELS 389
Query: 543 LGRGSTAAIVGEGLPAITPVDPWDGKDGEMPKEEEIDLSELGIEE 677
GRGSTA + G P I +PWDG+DGE+P E++IDLS++ +++
Sbjct: 390 FGRGSTAPVGGGAFPTIVEREPWDGRDGELPVEDDIDLSDVELDD 434
Score = 51.6 bits (122), Expect = 3e-06
Identities = 24/51 (47%), Positives = 34/51 (66%)
Frame = +3
Query: 9 VKLGALDATVHNAVASRYGVQGYPTIKFFPAGVKTGEAQEYDGGRSSSDIV 161
VK+GA+DA H+++ +YGVQG+PTIK F G ++Y GGR+ IV
Sbjct: 76 VKVGAVDADKHHSLGGQYGVQGFPTIKIF--GSNKNRPEDYQGGRTGEAIV 124
>sp|P38660|PDIA6_MESAU Protein disulfide-isomerase A6 precursor (Protein disulfide
isomerase P5)
Length = 439
Score = 253 bits (645), Expect = 6e-67
Identities = 127/232 (54%), Positives = 165/232 (71%), Gaps = 2/232 (0%)
Frame = +3
Query: 3 GKVKLGALDATVHNAVASRYGVQGYPTIKFFPAGVKTGEAQ-EYDGGRSSSDIVRWASDK 179
GKVKL A+DATV+ +A+RYG++G+PTIK F + GEA +YDGGR+ SDIV A D
Sbjct: 213 GKVKLAAVDATVNQVLANRYGIRGFPTIKIF----QKGEAPVDYDGGRTRSDIVSRALDL 268
Query: 180 VVEQLPAPELKEITNQDVLKSTCEDHQLCVIAVLPNILDCQSACRNSYLDTIRVSSDKYK 359
+ P PEL EI N+DV K CE+HQLCV+AVLP+ILD A RNSYL+ + +DKYK
Sbjct: 269 FSDNAPPPELLEIINEDVAKKMCEEHQLCVVAVLPHILDT-GAARNSYLEILLKLADKYK 327
Query: 360 ANRWGWCWTEAMKLPAIEKAFGIGGFGYPALVVVNVRKMKYATLTGPFSQTGIQDFVRDV 539
WGW WTEA +E A GIGGFGYPA+ +N RKMK+A L G FS+ GI +F+R++
Sbjct: 328 KKMWGWLWTEAGAQSELENALGIGGFGYPAMARINARKMKFALLKGSFSEQGINEFLREL 387
Query: 540 SLGRGSTAAIVGEGLPAITPVDPWDGKDGEMPKEEEIDLSELGIEE-PKSEL 692
S GR STA + G PAIT +PWDG+DGE+P E++IDLS++ +++ K EL
Sbjct: 388 SFGRASTAPVGGGSFPAITAREPWDGRDGELPVEDDIDLSDVELDDLEKDEL 439
Score = 52.4 bits (124), Expect = 1e-06
Identities = 24/51 (47%), Positives = 34/51 (66%)
Frame = +3
Query: 9 VKLGALDATVHNAVASRYGVQGYPTIKFFPAGVKTGEAQEYDGGRSSSDIV 161
VK+GA+DA H ++ +YGVQG+PTIK F G + ++Y GGR+ IV
Sbjct: 76 VKVGAVDADKHQSLGGQYGVQGFPTIKIF--GANKNKPEDYQGGRTGEAIV 124
>sp|Q17770|PDI2_CAEEL Protein disulfide-isomerase 2 precursor (PDI 1) (Prolyl
4-hydroxylase beta subunit)
Length = 493
Score = 67.0 bits (162), Expect = 6e-11
Identities = 35/88 (39%), Positives = 49/88 (55%)
Frame = +3
Query: 9 VKLGALDATVHNAVASRYGVQGYPTIKFFPAGVKTGEAQEYDGGRSSSDIVRWASDKVVE 188
+KLG LDATVH V+S++ V+GYPT+K F + G+ QEY+GGR I+ W K
Sbjct: 76 IKLGKLDATVHGEVSSKFEVRGYPTLKLF----RNGKPQEYNGGRDHDSIIAWLKKKT-- 129
Query: 189 QLPAPELKEITNQDVLKSTCEDHQLCVI 272
P K + + D +K E + VI
Sbjct: 130 ---GPVAKPLADADAVKELQESADVVVI 154
Score = 31.2 bits (69), Expect = 3.6
Identities = 18/66 (27%), Positives = 33/66 (50%)
Frame = +3
Query: 9 VKLGALDATVHNAVASRYGVQGYPTIKFFPAGVKTGEAQEYDGGRSSSDIVRWASDKVVE 188
+ + +D+T++ + +Q +PTIKFFPAG + + +Y G R+ ++ E
Sbjct: 416 IVIAKMDSTLNEVEDVK--IQSFPTIKFFPAG--SNKVVDYTGDRTIEGFTKFLETNGKE 471
Query: 189 QLPAPE 206
A E
Sbjct: 472 GAGASE 477
>sp|P54399|PDI_DROME Protein disulfide-isomerase precursor (PDI)
Length = 496
Score = 64.7 bits (156), Expect = 3e-10
Identities = 31/94 (32%), Positives = 54/94 (57%)
Frame = +3
Query: 9 VKLGALDATVHNAVASRYGVQGYPTIKFFPAGVKTGEAQEYDGGRSSSDIVRWASDKVVE 188
+KL +DATV +A +Y V+GYPT+KFF ++G EY GGR ++DI+ W + K
Sbjct: 80 IKLAKVDATVEGELAEQYAVRGYPTLKFF----RSGSPVEYSGGRQAADIIAWVTKKT-- 133
Query: 189 QLPAPELKEITNQDVLKSTCEDHQLCVIAVLPNI 290
P K++T+ + +D+++ +I ++
Sbjct: 134 ---GPPAKDLTSVADAEQFLKDNEIAIIGFFKDL 164
>sp|P21195|PDIA1_RABIT Protein disulfide-isomerase precursor (PDI) (Prolyl 4-hydroxylase
beta subunit) (Cellular thyroid hormone binding protein)
(p55)
Length = 509
Score = 58.9 bits (141), Expect = 2e-08
Identities = 31/94 (32%), Positives = 50/94 (53%)
Frame = +3
Query: 9 VKLGALDATVHNAVASRYGVQGYPTIKFFPAGVKTGEAQEYDGGRSSSDIVRWASDKVVE 188
++L +DAT + +A +YGV+GYPTIKFF G T +EY GR + DIV W ++
Sbjct: 78 IRLAKVDATEESDLAQQYGVRGYPTIKFFKNG-DTASPKEYTAGREADDIVNW-----LK 131
Query: 189 QLPAPELKEITNQDVLKSTCEDHQLCVIAVLPNI 290
+ P + + +S E ++ VI ++
Sbjct: 132 KRTGPAATTLADSAAAESLVESSEVAVIGFFKDV 165
>sp|P09103|PDIA1_MOUSE Protein disulfide-isomerase precursor (PDI) (Prolyl 4-hydroxylase
beta subunit) (Cellular thyroid hormone binding protein)
(p55) (Erp59)
Length = 509
Score = 57.8 bits (138), Expect = 4e-08
Identities = 30/95 (31%), Positives = 52/95 (54%)
Frame = +3
Query: 6 KVKLGALDATVHNAVASRYGVQGYPTIKFFPAGVKTGEAQEYDGGRSSSDIVRWASDKVV 185
+++L +DAT + +A +YGV+GYPTIKFF G T +EY GR + DIV W +
Sbjct: 78 EIRLAKVDATEESDLAQQYGVRGYPTIKFFKNG-DTASPKEYTAGREADDIVNW-----L 131
Query: 186 EQLPAPELKEITNQDVLKSTCEDHQLCVIAVLPNI 290
++ P +++ +S + ++ VI ++
Sbjct: 132 KKRTGPAATTLSDTAAAESLVDSSEVTVIGFFKDV 166
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 100,639,855
Number of Sequences: 369166
Number of extensions: 2092604
Number of successful extensions: 6592
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6184
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6549
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7859674995
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)