Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_020_J13
(437 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O62667|S28A1_PIG Sodium/nucleoside cotransporter 1 (Na(+... 145 4e-35
sp|Q62773|S28A2_RAT Sodium/nucleoside cotransporter 2 (Na(+... 144 6e-35
sp|Q62674|S28A1_RAT Sodium/nucleoside cotransporter 1 (Na(+... 144 8e-35
sp|O00337|S28A1_HUMAN Sodium/nucleoside cotransporter 1 (Na... 144 1e-34
sp|O88627|S28A2_MOUSE Sodium/nucleoside cotransporter 2 (Na... 144 1e-34
sp|O43868|S28A2_HUMAN Sodium/nucleoside cotransporter 2 (Na... 140 9e-34
sp|Q9MZT2|S28A1_RABIT Sodium/nucleoside cotransporter 1 (Na... 134 1e-31
sp|P44742|Y519_HAEIN Hypothetical protein HI0519 122 4e-28
sp|P33024|YEIM_ECOLI Inner membrane transport protein yeiM 94 1e-19
sp|P33021|YEIJ_ECOLI Inner membrane transport protein yeiJ 88 7e-18
>sp|O62667|S28A1_PIG Sodium/nucleoside cotransporter 1 (Na(+)/nucleoside cotransporter
1) (Sodium-coupled nucleoside transporter 1)
(Concentrative nucleoside transporter 1) (CNT 1)
Length = 647
Score = 145 bits (366), Expect = 4e-35
Identities = 78/145 (53%), Positives = 97/145 (66%)
Frame = +2
Query: 2 HTEAGSIMVFGKDFAQHYFAFRILPIIVFLSSIMSXXXXXXXXXXXXXXXXTAMQKFMGT 181
+TEAGS VFG+ + FAF++LPIIVF S MS MQ MGT
Sbjct: 243 YTEAGSSFVFGEALVKDVFAFQVLPIIVFFSCAMSVLYYVGLMQWVILKISWLMQATMGT 302
Query: 182 TCGESLNAAANIFLGMTESPLLIKPLLNRMTNSELHAIMTGGFATIAGSVLGAYIGYGAP 361
T E+L+ A NIF+ TE+PLLI+P L MT SE+H +MTGG+ATIAGS+LGAYI +G
Sbjct: 303 TATETLSVAGNIFVSQTEAPLLIRPYLADMTLSEIHVVMTGGYATIAGSLLGAYISFGID 362
Query: 362 ANHLISASVMSAPAALAISKLMCPE 436
A LI+ASVM+AP ALA+SKL+ PE
Sbjct: 363 AASLIAASVMAAPCALALSKLVYPE 387
>sp|Q62773|S28A2_RAT Sodium/nucleoside cotransporter 2 (Na(+)/nucleoside cotransporter
2) (Sodium-coupled nucleoside transporter 2)
(Concentrative nucleoside transporter 2) (CNT 2)
(Sodium/purine nucleoside cotransporter) (SPNT) (RCNT2)
Length = 659
Score = 144 bits (364), Expect = 6e-35
Identities = 78/145 (53%), Positives = 96/145 (66%)
Frame = +2
Query: 2 HTEAGSIMVFGKDFAQHYFAFRILPIIVFLSSIMSXXXXXXXXXXXXXXXXTAMQKFMGT 181
+T GS VFG Q FAF+ LPII+F +MS +Q MGT
Sbjct: 240 YTVEGSSFVFGDTLVQSVFAFQSLPIIIFFGCVMSILYYLGLVQWVIQKIAWFLQITMGT 299
Query: 182 TCGESLNAAANIFLGMTESPLLIKPLLNRMTNSELHAIMTGGFATIAGSVLGAYIGYGAP 361
T E+L A NIF+GMTE+PLLI+P L MT SE+HA+MTGGFATIAG+VLGA+I +G
Sbjct: 300 TAAETLAVAGNIFVGMTEAPLLIRPYLADMTLSEIHAVMTGGFATIAGTVLGAFISFGID 359
Query: 362 ANHLISASVMSAPAALAISKLMCPE 436
A+ LISASVM+AP ALA+SKL+ PE
Sbjct: 360 ASSLISASVMAAPCALALSKLVYPE 384
>sp|Q62674|S28A1_RAT Sodium/nucleoside cotransporter 1 (Na(+)/nucleoside cotransporter
1) (Sodium-coupled nucleoside transporter 1)
(Concentrative nucleoside transporter 1) (CNT 1)
Length = 648
Score = 144 bits (363), Expect = 8e-35
Identities = 76/145 (52%), Positives = 98/145 (67%)
Frame = +2
Query: 2 HTEAGSIMVFGKDFAQHYFAFRILPIIVFLSSIMSXXXXXXXXXXXXXXXXTAMQKFMGT 181
+TEAGS VFG+ + FAF++LPII+F S +MS MQ MGT
Sbjct: 244 YTEAGSSFVFGEALVKDVFAFQVLPIIIFFSCVMSVLYYLGLMQWVILKIAWLMQVTMGT 303
Query: 182 TCGESLNAAANIFLGMTESPLLIKPLLNRMTNSELHAIMTGGFATIAGSVLGAYIGYGAP 361
+ E+L+ A NIF+ TE+PLLI+P L MT SE+H +MTGG+ATIAGS+LGAYI +G
Sbjct: 304 SATETLSVAGNIFVSQTEAPLLIRPYLADMTLSEVHVVMTGGYATIAGSLLGAYISFGID 363
Query: 362 ANHLISASVMSAPAALAISKLMCPE 436
A LI+ASVM+AP ALA+SKL+ PE
Sbjct: 364 AASLIAASVMAAPCALALSKLVYPE 388
>sp|O00337|S28A1_HUMAN Sodium/nucleoside cotransporter 1 (Na(+)/nucleoside cotransporter
1) (Sodium-coupled nucleoside transporter 1)
(Concentrative nucleoside transporter 1) (CNT 1) (hCNT1)
Length = 649
Score = 144 bits (362), Expect = 1e-34
Identities = 76/145 (52%), Positives = 98/145 (67%)
Frame = +2
Query: 2 HTEAGSIMVFGKDFAQHYFAFRILPIIVFLSSIMSXXXXXXXXXXXXXXXXTAMQKFMGT 181
+T+AGS VFG+ + FAF++LPIIVF S ++S MQ MGT
Sbjct: 244 YTKAGSSFVFGEALVKDVFAFQVLPIIVFFSCVISVLYHVGLMQWVILKIAWLMQVTMGT 303
Query: 182 TCGESLNAAANIFLGMTESPLLIKPLLNRMTNSELHAIMTGGFATIAGSVLGAYIGYGAP 361
T E+L+ A NIF+ TE+PLLI+P L MT SE+H +MTGG+ATIAGS+LGAYI +G
Sbjct: 304 TATETLSVAGNIFVSQTEAPLLIRPYLADMTLSEVHVVMTGGYATIAGSLLGAYISFGID 363
Query: 362 ANHLISASVMSAPAALAISKLMCPE 436
A LI+ASVM+AP ALA+SKL+ PE
Sbjct: 364 ATSLIAASVMAAPCALALSKLVYPE 388
>sp|O88627|S28A2_MOUSE Sodium/nucleoside cotransporter 2 (Na(+)/nucleoside cotransporter
2) (Sodium-coupled nucleoside transporter 2)
(Concentrative nucleoside transporter 2) (CNT 2)
(Sodium/purine nucleoside cotransporter) (SPNT)
Length = 660
Score = 144 bits (362), Expect = 1e-34
Identities = 77/145 (53%), Positives = 96/145 (66%)
Frame = +2
Query: 2 HTEAGSIMVFGKDFAQHYFAFRILPIIVFLSSIMSXXXXXXXXXXXXXXXXTAMQKFMGT 181
+T GS VFG Q+ FAF+ LPII+F +MS +Q MGT
Sbjct: 241 YTVEGSSFVFGDTLVQNVFAFQSLPIIIFFGCVMSILYYLGLVQWVIQKVAWFLQITMGT 300
Query: 182 TCGESLNAAANIFLGMTESPLLIKPLLNRMTNSELHAIMTGGFATIAGSVLGAYIGYGAP 361
T E+L A NIF+GMTE+PLLI+P L MT SE+H +MTGGFATIAG+VLGA+I +G
Sbjct: 301 TAAETLAVAGNIFVGMTEAPLLIRPYLADMTISEIHTVMTGGFATIAGTVLGAFISFGID 360
Query: 362 ANHLISASVMSAPAALAISKLMCPE 436
A+ LISASVM+AP ALA+SKL+ PE
Sbjct: 361 ASSLISASVMAAPCALALSKLVYPE 385
>sp|O43868|S28A2_HUMAN Sodium/nucleoside cotransporter 2 (Na(+)/nucleoside cotransporter
2) (Sodium-coupled nucleoside transporter 2)
(Concentrative nucleoside transporter 2) (CNT 2) (hCNT2)
(Sodium/purine nucleoside co-transporter) (SPNT)
Length = 658
Score = 140 bits (354), Expect = 9e-34
Identities = 76/145 (52%), Positives = 95/145 (65%)
Frame = +2
Query: 2 HTEAGSIMVFGKDFAQHYFAFRILPIIVFLSSIMSXXXXXXXXXXXXXXXXTAMQKFMGT 181
+T AGS VFG + FAF+ LPII+F ++S +Q MGT
Sbjct: 239 YTVAGSSFVFGDTLVKDVFAFQALPIIIFFGCVVSILYYLGLVQWVVQKVAWFLQITMGT 298
Query: 182 TCGESLNAAANIFLGMTESPLLIKPLLNRMTNSELHAIMTGGFATIAGSVLGAYIGYGAP 361
T E+L A NIF+GMTE+PLLI+P L MT SE+HA+MTGGFATI+G+VLGA+I +G
Sbjct: 299 TATETLAVAGNIFVGMTEAPLLIRPYLGDMTLSEIHAVMTGGFATISGTVLGAFIAFGVD 358
Query: 362 ANHLISASVMSAPAALAISKLMCPE 436
A+ LISASVM+AP ALA SKL PE
Sbjct: 359 ASSLISASVMAAPCALASSKLAYPE 383
>sp|Q9MZT2|S28A1_RABIT Sodium/nucleoside cotransporter 1 (Na(+)/nucleoside cotransporter
1) (Sodium-coupled nucleoside transporter 1)
(Concentrative nucleoside transporter 1) (CNT 1)
Length = 658
Score = 134 bits (336), Expect = 1e-31
Identities = 73/145 (50%), Positives = 92/145 (63%)
Frame = +2
Query: 2 HTEAGSIMVFGKDFAQHYFAFRILPIIVFLSSIMSXXXXXXXXXXXXXXXXTAMQKFMGT 181
+T AGS V G FAF+ LPII+F +MS +Q M T
Sbjct: 239 YTVAGSSFVLGDTLVNDVFAFQSLPIIIFFGCVMSILYYLGLVQWVVQKIAWFLQVTMRT 298
Query: 182 TCGESLNAAANIFLGMTESPLLIKPLLNRMTNSELHAIMTGGFATIAGSVLGAYIGYGAP 361
T E+L A NIF+GMTE+PLLI+P L +T SE+HA+MT GFATI+G+VLGA+I +G
Sbjct: 299 TATETLAVAGNIFVGMTEAPLLIRPYLADLTLSEIHAVMTSGFATISGTVLGAFISFGID 358
Query: 362 ANHLISASVMSAPAALAISKLMCPE 436
A+ LISASVM AP ALA+SKL+ PE
Sbjct: 359 ASSLISASVMGAPCALALSKLVYPE 383
>sp|P44742|Y519_HAEIN Hypothetical protein HI0519
Length = 417
Score = 122 bits (305), Expect = 4e-28
Identities = 62/127 (48%), Positives = 84/127 (66%)
Frame = +2
Query: 56 FAFRILPIIVFLSSIMSXXXXXXXXXXXXXXXXTAMQKFMGTTCGESLNAAANIFLGMTE 235
FA ++LPIIVF S ++S A+QK +GT+ ES++AAANIF+G TE
Sbjct: 92 FAVKVLPIIVFFSGLISVLYYLGIMQVVIKVLGGALQKALGTSKAESMSAAANIFVGQTE 151
Query: 236 SPLLIKPLLNRMTNSELHAIMTGGFATIAGSVLGAYIGYGAPANHLISASVMSAPAALAI 415
+PL+++P + MT SEL AIM GG A+IAGSV+ Y G G P +LI+AS M+APA L
Sbjct: 152 APLVVRPYIKNMTQSELFAIMVGGTASIAGSVMAGYAGMGVPLTYLIAASFMAAPAGLLF 211
Query: 416 SKLMCPE 436
+KLM P+
Sbjct: 212 AKLMFPQ 218
>sp|P33024|YEIM_ECOLI Inner membrane transport protein yeiM
Length = 416
Score = 94.0 bits (232), Expect = 1e-19
Identities = 49/154 (31%), Positives = 83/154 (53%), Gaps = 10/154 (6%)
Frame = +2
Query: 2 HTEAGSIMVFGK----------DFAQHYFAFRILPIIVFLSSIMSXXXXXXXXXXXXXXX 151
+++AGS +FG D A FAFR+LP I+F+++++S
Sbjct: 69 YSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVLPAIIFVTALISLLYYIGVMGLLIRIL 128
Query: 152 XTAMQKFMGTTCGESLNAAANIFLGMTESPLLIKPLLNRMTNSELHAIMTGGFATIAGSV 331
+ QK + + ES A IFLG E P ++KP ++RM +EL + G A+IAGS+
Sbjct: 129 GSIFQKALNISKIESFVAVTTIFLGQNEIPAIVKPFIDRMNRNELFTAICSGMASIAGSM 188
Query: 332 LGAYIGYGAPANHLISASVMSAPAALAISKLMCP 433
+ Y G G P ++L++AS+M+ P + ++++ P
Sbjct: 189 MIGYAGMGVPIDYLLAASLMAIPGGILFARILSP 222
>sp|P33021|YEIJ_ECOLI Inner membrane transport protein yeiJ
Length = 416
Score = 88.2 bits (217), Expect = 7e-18
Identities = 47/154 (30%), Positives = 78/154 (50%), Gaps = 10/154 (6%)
Frame = +2
Query: 2 HTEAGSIMVFGK----------DFAQHYFAFRILPIIVFLSSIMSXXXXXXXXXXXXXXX 151
+++AGS +FG D A F FR+LP I+F+++++S
Sbjct: 69 YSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVLPAIIFVTALVSILYYIGVMGILIRIL 128
Query: 152 XTAMQKFMGTTCGESLNAAANIFLGMTESPLLIKPLLNRMTNSELHAIMTGGFATIAGSV 331
QK + + ES A IFLG E P ++KP ++R+ +EL + G A+IAGS
Sbjct: 129 GGIFQKALNISKIESFVAVTTIFLGQNEIPAIVKPFIDRLNRNELFTAICSGMASIAGST 188
Query: 332 LGAYIGYGAPANHLISASVMSAPAALAISKLMCP 433
+ Y G P +L++AS+M+ P + ++L+ P
Sbjct: 189 MIGYAALGVPVEYLLAASLMAIPGGILFARLLSP 222
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 50,648,949
Number of Sequences: 369166
Number of extensions: 971228
Number of successful extensions: 2152
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2110
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2140
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2244666600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)