Planarian EST Database


Dr_sW_020_J08

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_020_J08
         (904 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9UHB6|EPLIN_HUMAN  Epithelial protein lost in neoplasm         86   1e-16
sp|Q9ERG0|EPLIN_MOUSE  Epithelial protein lost in neoplasm (...    86   1e-16
sp|P29675|SF3_HELAN  Pollen-specific protein SF3                   69   2e-11
sp|Q14847|LASP1_HUMAN  LIM and SH3 domain protein 1 (LASP-1)...    63   1e-09
sp|Q99MZ8|LASP1_RAT  LIM and SH3 domain protein 1 (LASP-1)         63   1e-09
sp|Q61792|LASP1_MOUSE  LIM and SH3 domain protein 1 (LASP-1)...    63   1e-09
sp|O94851|MICA2_HUMAN  Protein MICAL-2                             62   2e-09
sp|P36201|CRIP2_RAT  Cysteine-rich protein 2 (CRP2) (Protein...    62   2e-09
sp|Q9DCT8|CRIP2_MOUSE  Cysteine-rich protein 2 (CRP2) (Heart...    62   2e-09
sp|P34416|LASP1_CAEEL  LIM and SH3 domain protein F42H10.3         62   3e-09
>sp|Q9UHB6|EPLIN_HUMAN Epithelial protein lost in neoplasm
          Length = 759

 Score = 86.3 bits (212), Expect = 1e-16
 Identities = 34/65 (52%), Positives = 45/65 (69%)
 Frame = +3

Query: 186 CCICNKKVYPMEKLSTNDKIFHKTCFRCTKCKNALSTGNFSFNHGKPYCTPHYVELFKKR 365
           C  C K VYPME+L  N ++FH +CFRC+ C N LS G ++  HG+ YC PH+ +LFK +
Sbjct: 390 CVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSK 449

Query: 366 GRYDE 380
           G YDE
Sbjct: 450 GNYDE 454
>sp|Q9ERG0|EPLIN_MOUSE Epithelial protein lost in neoplasm (mEPLIN)
          Length = 753

 Score = 86.3 bits (212), Expect = 1e-16
 Identities = 34/65 (52%), Positives = 45/65 (69%)
 Frame = +3

Query: 186 CCICNKKVYPMEKLSTNDKIFHKTCFRCTKCKNALSTGNFSFNHGKPYCTPHYVELFKKR 365
           C  C K VYPME+L  N ++FH +CFRC+ C N LS G ++  HG+ YC PH+ +LFK +
Sbjct: 388 CVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSK 447

Query: 366 GRYDE 380
           G YDE
Sbjct: 448 GNYDE 452
>sp|P29675|SF3_HELAN Pollen-specific protein SF3
          Length = 219

 Score = 68.6 bits (166), Expect = 2e-11
 Identities = 28/72 (38%), Positives = 41/72 (56%)
 Frame = +3

Query: 174 TNGDCCICNKKVYPMEKLSTNDKIFHKTCFRCTKCKNALSTGNFSFNHGKPYCTPHYVEL 353
           T   C +C K VY ++KL  N +++HK CFRC  C + L   NF+   G  YC  H+ +L
Sbjct: 7   TTQKCTVCEKTVYLVDKLVANQRVYHKACFRCHHCNSTLKLSNFNSFDGVVYCRHHFDQL 66

Query: 354 FKKRGRYDEIDD 389
           FK+ G  ++  D
Sbjct: 67  FKRTGSLEKSFD 78

 Score = 65.5 bits (158), Expect = 2e-10
 Identities = 32/115 (27%), Positives = 55/115 (47%)
 Frame = +3

Query: 174 TNGDCCICNKKVYPMEKLSTNDKIFHKTCFRCTKCKNALSTGNFSFNHGKPYCTPHYVEL 353
           T   C  C K VYP+E++  +   +H+ CF+C      +S  N+  + G+ YC  H+++L
Sbjct: 106 TRDKCNACAKIVYPIERVKVDGTAYHRACFKCCHGGCTISPSNYIAHEGRLYCKHHHIQL 165

Query: 354 FKKRGRYDEIDDNIENEILATNDISDVLTDELPAPDTTKNILAKFRDLQTGGNSN 518
           FKK+G Y +++  +E  + A            PA   T+N   +  + Q     N
Sbjct: 166 FKKKGNYSQLE--VEETVAA------------PAESETQNTETQNAETQNADTQN 206
>sp|Q14847|LASP1_HUMAN LIM and SH3 domain protein 1 (LASP-1) (MLN 50)
          Length = 261

 Score = 63.2 bits (152), Expect = 1e-09
 Identities = 26/56 (46%), Positives = 32/56 (57%)
 Frame = +3

Query: 177 NGDCCICNKKVYPMEKLSTNDKIFHKTCFRCTKCKNALSTGNFSFNHGKPYCTPHY 344
           N +C  C K VYP EK++  DK +HK CF C  CK  L+  N+     KPYC  HY
Sbjct: 2   NPNCARCGKIVYPTEKVNCLDKFWHKACFHCETCKMTLNMKNYKGYEKKPYCNAHY 57
>sp|Q99MZ8|LASP1_RAT LIM and SH3 domain protein 1 (LASP-1)
          Length = 263

 Score = 63.2 bits (152), Expect = 1e-09
 Identities = 26/56 (46%), Positives = 32/56 (57%)
 Frame = +3

Query: 177 NGDCCICNKKVYPMEKLSTNDKIFHKTCFRCTKCKNALSTGNFSFNHGKPYCTPHY 344
           N +C  C K VYP EK++  DK +HK CF C  CK  L+  N+     KPYC  HY
Sbjct: 2   NPNCARCGKIVYPTEKVNCLDKFWHKACFHCETCKMTLNMKNYKGYEKKPYCNAHY 57
>sp|Q61792|LASP1_MOUSE LIM and SH3 domain protein 1 (LASP-1) (MLN 50)
          Length = 263

 Score = 62.8 bits (151), Expect = 1e-09
 Identities = 26/56 (46%), Positives = 32/56 (57%)
 Frame = +3

Query: 177 NGDCCICNKKVYPMEKLSTNDKIFHKTCFRCTKCKNALSTGNFSFNHGKPYCTPHY 344
           N +C  C K VYP EK++  DK +HK CF C  CK  L+  N+     KPYC  HY
Sbjct: 2   NPNCARCGKIVYPTEKVNCLDKYWHKACFHCETCKMTLNMKNYKGYEKKPYCNAHY 57
>sp|O94851|MICA2_HUMAN Protein MICAL-2
          Length = 1124

 Score = 62.4 bits (150), Expect = 2e-09
 Identities = 33/85 (38%), Positives = 44/85 (51%), Gaps = 6/85 (7%)
 Frame = +3

Query: 168  NLTNGDCC-ICNKKVYPMEKLSTNDKIFHKTCFRCTKCKNALSTGNFSF--NHGKPYCTP 338
            NL   D C  C K+VY ME+LS     FH+ CFRC+ C   L    ++F  + GK YC P
Sbjct: 995  NLGGSDTCYFCKKRVYVMERLSAEGHFFHRECFRCSICATTLRLAAYTFDCDEGKFYCKP 1054

Query: 339  HYVEL---FKKRGRYDEIDDNIENE 404
            H++      K+R R  E+    E E
Sbjct: 1055 HFIHCKTNSKQRKRRAELKQQREEE 1079
>sp|P36201|CRIP2_RAT Cysteine-rich protein 2 (CRP2) (Protein ESP1)
          Length = 208

 Score = 62.0 bits (149), Expect = 2e-09
 Identities = 33/93 (35%), Positives = 51/93 (54%), Gaps = 1/93 (1%)
 Frame = +3

Query: 93  KQKVTYSPTKGKDVKSLSSTYGHEGNLTNGDCCICNKKVYPMEKLSTNDKIFHKTCFRCT 272
           +++ T  P KG    S  +T+  E N+    C  CNK+VY  EK+++  K +H+ C RC 
Sbjct: 99  EERKTSGPPKGPSKASSVTTFTGEPNM----CPRCNKRVYFAEKVTSLGKDWHRPCLRCE 154

Query: 273 KCKNALSTGNFSFNHGKPYC-TPHYVELFKKRG 368
           +C   L+ G  + + G+PYC  P Y  LF  +G
Sbjct: 155 RCSKTLTPGGHAEHDGQPYCHKPCYGILFGPKG 187

 Score = 53.9 bits (128), Expect = 6e-07
 Identities = 25/62 (40%), Positives = 36/62 (58%), Gaps = 1/62 (1%)
 Frame = +3

Query: 186 CCICNKKVYPMEKLSTNDKIFHKTCFRCTKCKNALSTGNFSFNHGKPYC-TPHYVELFKK 362
           C  C+K VY  EK+S+  K +HK C +C +C   L+ G  + + GKP+C  P Y  LF  
Sbjct: 5   CPKCDKTVYFAEKVSSLGKDWHKFCLKCERCNKTLTPGGHAEHDGKPFCHKPCYATLFGP 64

Query: 363 RG 368
           +G
Sbjct: 65  KG 66
>sp|Q9DCT8|CRIP2_MOUSE Cysteine-rich protein 2 (CRP2) (Heart LIM protein)
          Length = 208

 Score = 62.0 bits (149), Expect = 2e-09
 Identities = 33/93 (35%), Positives = 51/93 (54%), Gaps = 1/93 (1%)
 Frame = +3

Query: 93  KQKVTYSPTKGKDVKSLSSTYGHEGNLTNGDCCICNKKVYPMEKLSTNDKIFHKTCFRCT 272
           +++ T  P KG    S  +T+  E N+    C  CNK+VY  EK+++  K +H+ C RC 
Sbjct: 99  EERKTSGPPKGPSKASSVTTFTGEPNM----CPRCNKRVYFAEKVTSLGKDWHRPCLRCE 154

Query: 273 KCKNALSTGNFSFNHGKPYC-TPHYVELFKKRG 368
           +C   L+ G  + + G+PYC  P Y  LF  +G
Sbjct: 155 RCSKTLTPGGHAEHDGQPYCHKPCYGILFGPKG 187

 Score = 53.9 bits (128), Expect = 6e-07
 Identities = 25/62 (40%), Positives = 36/62 (58%), Gaps = 1/62 (1%)
 Frame = +3

Query: 186 CCICNKKVYPMEKLSTNDKIFHKTCFRCTKCKNALSTGNFSFNHGKPYC-TPHYVELFKK 362
           C  C+K VY  EK+S+  K +HK C +C +C   L+ G  + + GKP+C  P Y  LF  
Sbjct: 5   CPKCDKTVYFAEKVSSLGKDWHKFCLKCERCNKTLTPGGHAEHDGKPFCHKPCYATLFGP 64

Query: 363 RG 368
           +G
Sbjct: 65  KG 66
>sp|P34416|LASP1_CAEEL LIM and SH3 domain protein F42H10.3
          Length = 335

 Score = 61.6 bits (148), Expect = 3e-09
 Identities = 24/50 (48%), Positives = 32/50 (64%)
 Frame = +3

Query: 195 CNKKVYPMEKLSTNDKIFHKTCFRCTKCKNALSTGNFSFNHGKPYCTPHY 344
           C K VYP+E+L   DK++HK CF+CT C   L+  N+     +PYC PHY
Sbjct: 10  CGKTVYPVEELKCLDKVWHKQCFKCTVCGMTLNMKNYKGYDKRPYCDPHY 59
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 99,449,789
Number of Sequences: 369166
Number of extensions: 2135414
Number of successful extensions: 6256
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5837
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6248
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9126484700
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)