Planarian EST Database


Dr_sW_020_J07

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_020_J07
         (525 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P26624|GST28_SCHJA  Glutathione S-transferase 28 kDa (GST...    92   6e-19
sp|P09792|GST28_SCHMA  Glutathione S-transferase 28 kDa (GST...    89   8e-18
sp|P46088|GST_OMMSL  Glutathione S-transferase (GST class-si...    87   2e-17
sp|P30114|GST28_SCHHA  Glutathione S-transferase 28 kDa (GST...    84   2e-16
sp|P30113|GST28_SCHBO  Glutathione S-transferase 28 kDa (GST...    80   2e-15
sp|O73888|PTGD2_CHICK  Glutathione-requiring prostaglandin D...    80   2e-15
sp|O18598|GST1_BLAGE  Glutathione S-transferase (GST class-s...    80   4e-15
sp|P91253|GST7_CAEEL  Probable glutathione S-transferase 7 (...    78   1e-14
sp|P46429|GST2_MANSE  Glutathione S-transferase 2 (GST class...    76   6e-14
sp|P91254|GST8_CAEEL  Probable glutathione S-transferase 8 (...    75   1e-13
>sp|P26624|GST28_SCHJA Glutathione S-transferase 28 kDa (GST 28) (SJ28 antigen) (GST
           class-mu)
          Length = 206

 Score = 92.4 bits (228), Expect = 6e-19
 Identities = 55/164 (33%), Positives = 93/164 (56%), Gaps = 9/164 (5%)
 Frame = +3

Query: 3   PTTPTGKLPVVEYEGKK-----LTQSMAIARAIARKYGFMGEDDKEYYLIERAIGQMVDV 167
           PT P G+LP+V+   K+     +++S+AIAR IARK+  MG+ D EYY+IE+ IGQ+ DV
Sbjct: 41  PTIPGGRLPIVKITDKRGDVKTMSESLAIARFIARKHNMMGDTDDEYYIIEKMIGQVEDV 100

Query: 168 LEGMYKIYFAPEEKKEDLRTEFVTTSGRDNLKSLEGFIKES--GFFVGEKITLAELFFVV 341
               +K    P E+KE +  E +       L+++   +KES     VG+K+TLA++  + 
Sbjct: 101 ESEYHKTLIKPPEEKEKISKEILNGKVPILLQAICETLKESTGNLTVGDKVTLADVVLIA 160

Query: 342 LTDYLEKLPK--LYDDFPKLKELRERVLKANTDVEDWVKTRPVT 467
             D++  L K  L   +P++ + R+ +L  +  +  ++  R  T
Sbjct: 161 SIDHITDLDKEFLTGKYPEIHKHRKHLLATSPKLAKYLSERHAT 204
>sp|P09792|GST28_SCHMA Glutathione S-transferase 28 kDa (GST 28) (SM28 antigen)
           (Protective 28 kDa antigen) (GST class-mu)
          Length = 211

 Score = 88.6 bits (218), Expect = 8e-18
 Identities = 55/164 (33%), Positives = 90/164 (54%), Gaps = 9/164 (5%)
 Frame = +3

Query: 3   PTTPTGKLPVVEYEG-----KKLTQSMAIARAIARKYGFMGEDDKEYYLIERAIGQMVDV 167
           PT P G+LP V+        K + +S+AIAR +A+K+  MGE D+EYY +E+ IGQ  DV
Sbjct: 46  PTIPGGRLPAVKVTDDHGHVKWMLESLAIARYMAKKHHMMGETDEEYYSVEKLIGQAEDV 105

Query: 168 LEGMYKIYFAPEEKKEDLRTEFVTTSGRDNLKSLEGFIKES--GFFVGEKITLAELFFVV 341
               +K    P+E+KE +  E +       L  +   +K S     VG+K+TLA+L  + 
Sbjct: 106 EHEYHKTLMKPQEEKEKITKEILNGKVPVLLNMICESLKGSTGKLAVGDKVTLADLVLIA 165

Query: 342 LTDYLEKLPK--LYDDFPKLKELRERVLKANTDVEDWVKTRPVT 467
           + D++  L K  L   +P++ + RE +L ++  +  ++  RP T
Sbjct: 166 VIDHVTDLDKGFLTGKYPEIHKHRENLLASSPRLAKYLSNRPAT 209
>sp|P46088|GST_OMMSL Glutathione S-transferase (GST class-sigma)
          Length = 203

 Score = 87.4 bits (215), Expect = 2e-17
 Identities = 56/160 (35%), Positives = 89/160 (55%), Gaps = 5/160 (3%)
 Frame = +3

Query: 6   TTPTGKLPVVEYEGKKLTQSMAIARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYK 185
           T  +  +PV++ +G K++QSM IAR +AR++G  G+   E Y ++     + D+   + K
Sbjct: 45  TMYSNAMPVLDIDGTKMSQSMCIARHLAREFGLDGKTSLEKYRVDEITETLQDIFNDVVK 104

Query: 186 IYFAPEEKKEDLRTEFVTTSGRDNLKSLEGFIKES----GFFVGEKITLAELF-FVVLTD 350
           I FAPE  KE ++  +  +  R     LEG +  +    GFFVG  +TLA+L  +V L  
Sbjct: 105 IKFAPEAAKEAVQQNYEKSCKR-LAPFLEGLLVSNGGGDGFFVGNSMTLADLHCYVALEV 163

Query: 351 YLEKLPKLYDDFPKLKELRERVLKANTDVEDWVKTRPVTD 470
            L+  P+L  D PK+  LR+RV +    +  ++K RPV D
Sbjct: 164 PLKHTPELLKDCPKIVALRKRVAEC-PKIAAYLKKRPVRD 202
>sp|P30114|GST28_SCHHA Glutathione S-transferase 28 kDa (GST 28) (GST class-mu)
          Length = 211

 Score = 84.0 bits (206), Expect = 2e-16
 Identities = 53/164 (32%), Positives = 90/164 (54%), Gaps = 9/164 (5%)
 Frame = +3

Query: 3   PTTPTGKLPVVEYEG-----KKLTQSMAIARAIARKYGFMGEDDKEYYLIERAIGQMVDV 167
           PT P G+LP V+        K + +S+AIAR +A+K+  MGE D+EYY +E+ IGQ+ D+
Sbjct: 46  PTIPGGRLPAVKITDNHGHVKWMLESLAIARYMAKKHHMMGETDEEYYNVEKLIGQVEDL 105

Query: 168 LEGMYKIYFAPEEKKEDLRTEFVTTSGRDNLKSLEGFIKES--GFFVGEKITLAELFFVV 341
               +K    PEE+K+ +  E +       L  +   +K S     VG+K+TLA+L  + 
Sbjct: 106 EHEYHKTLMKPEEEKQKITKEILNGKVPVLLDIICESLKASTGKLAVGDKVTLADLVLIA 165

Query: 342 LTDYLEKLPK--LYDDFPKLKELRERVLKANTDVEDWVKTRPVT 467
           + D++  L K  L   +P++ + RE +L ++  +  ++  R  T
Sbjct: 166 VIDHVTDLDKEFLTGKYPEIHKHRENLLASSPRLAKYLSDRAAT 209
>sp|P30113|GST28_SCHBO Glutathione S-transferase 28 kDa (GST 28) (GST class-mu)
          Length = 211

 Score = 80.5 bits (197), Expect = 2e-15
 Identities = 52/164 (31%), Positives = 88/164 (53%), Gaps = 9/164 (5%)
 Frame = +3

Query: 3   PTTPTGKLPVVEYEG-----KKLTQSMAIARAIARKYGFMGEDDKEYYLIERAIGQMVDV 167
           PT P G+LP V+        K + +S+AIAR +A+K+  MG  ++EYY +E+ IGQ  D+
Sbjct: 46  PTIPGGRLPAVKITDNHGHVKWMVESLAIARYMAKKHHMMGGTEEEYYNVEKLIGQAEDL 105

Query: 168 LEGMYKIYFAPEEKKEDLRTEFVTTSGRDNLKSLEGFIKES--GFFVGEKITLAELFFVV 341
               YK    PEE+K+ +  E +       L  +   +K S     VG+K+TLA+L  + 
Sbjct: 106 EHEYYKTLMKPEEEKQKIIKEILNGKVPVLLDIICESLKASTGKLAVGDKVTLADLVLIA 165

Query: 342 LTDYLEKLPK--LYDDFPKLKELRERVLKANTDVEDWVKTRPVT 467
           + D++  L K  L   +P++ + RE +L ++  +  ++  R  T
Sbjct: 166 VIDHVTDLDKEFLTGKYPEIHKHRENLLASSPRLAKYLSDRAAT 209
>sp|O73888|PTGD2_CHICK Glutathione-requiring prostaglandin D synthase
           (Glutathione-dependent PGD synthetase) (Prostaglandin-H2
           D-isomerase) (Hematopoietic prostaglandin D synthase)
           (H-PGDS)
          Length = 199

 Score = 80.5 bits (197), Expect = 2e-15
 Identities = 51/160 (31%), Positives = 87/160 (54%), Gaps = 5/160 (3%)
 Frame = +3

Query: 3   PTTPTGKLPVVEYEGKKLTQSMAIARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMY 182
           PT P GK+P++E +G  + QS+AIAR +AR+ G  G+   E  L +  +  + D     +
Sbjct: 44  PTIPFGKVPILEVDGVIIHQSLAIARYLARESGLAGQTPVEQALADAIVDTIDD-----F 98

Query: 183 KIYFAPEEKKEDLR----TEFVTTSGRDNLKSLEGFIKESGFFVGEKITLAELFF-VVLT 347
            + F   EK +D++     + +T    + LK L+ F+ +  +FVG+ +T A+ ++ V  T
Sbjct: 99  MMLFPWAEKNQDVKEKAFNDILTNKAPELLKDLDTFLGDKKWFVGKSVTWADFYWDVCST 158

Query: 348 DYLEKLPKLYDDFPKLKELRERVLKANTDVEDWVKTRPVT 467
             L     L D +P+L  LR+RV +A   +  W++ RP T
Sbjct: 159 TLLSYKADLADKYPRLLALRDRV-EALPAIAAWIQKRPKT 197
>sp|O18598|GST1_BLAGE Glutathione S-transferase (GST class-sigma) (Major allergen Bla g
           5)
          Length = 204

 Score = 79.7 bits (195), Expect = 4e-15
 Identities = 53/166 (31%), Positives = 86/166 (51%), Gaps = 9/166 (5%)
 Frame = +3

Query: 3   PTTPTGKLPVVEYEGKKLTQSMAIARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMY 182
           P+ P GK PV+E +GK+  QS+AI+R + +++G  G+DD E   I+  +  + D    + 
Sbjct: 45  PSMPFGKTPVLEIDGKQTHQSVAISRYLGKQFGLSGKDDWENLEIDMIVDTISDFRAAIA 104

Query: 183 KIYFAPEE-----KKEDLRTEFVT--TSGRDNLKSLEGFIKESGFFVGEKITLAELFFVV 341
             ++  +E     K + L+ E +   T   D +    G     G+    K+T A+ +FV 
Sbjct: 105 NYHYDADENSKQKKWDPLKKETIPYYTKKFDEVVKANG-----GYLAAGKLTWADFYFVA 159

Query: 342 LTDYLEKLPK--LYDDFPKLKELRERVLKANTDVEDWVKTRPVTDM 473
           + DYL  + K  L  + P LK LRE+VL     ++ WV  RP TD+
Sbjct: 160 ILDYLNHMAKEDLVANQPNLKALREKVLGLPA-IKAWVAKRPPTDL 204
>sp|P91253|GST7_CAEEL Probable glutathione S-transferase 7 (GST class-sigma)
          Length = 206

 Score = 77.8 bits (190), Expect = 1e-14
 Identities = 55/163 (33%), Positives = 85/163 (52%), Gaps = 8/163 (4%)
 Frame = +3

Query: 3   PTTPTGKLPVVEYEGKKLTQSMAIARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEG-- 176
           P+TP G+LP++E +GK L QS AIAR +AR++G  G+   E   +     Q  D L    
Sbjct: 44  PSTPFGQLPLLEVDGKVLAQSHAIARYLARQFGINGKCAWEEAQVNSVADQFKDYLNEVR 103

Query: 177 ---MYKIYFAPEEKKEDLRTEFVTTSGRDNLKSLEGFIKE--SGFFVGEKITLAELFFVV 341
              M K+ FA E   + L  +      + +      F+K   SG+ VG+ +T  +L    
Sbjct: 104 PYFMVKMGFA-EGDLDALAKDVFLPGFKKHYGFFANFLKSAGSGYLVGDSLTFVDLLVAQ 162

Query: 342 LT-DYLEKLPKLYDDFPKLKELRERVLKANTDVEDWVKTRPVT 467
            T D L     L D+FP+ K  +E+V  +N +++ W++TRPVT
Sbjct: 163 HTADLLAANAALLDEFPQFKAHQEKV-HSNANIKKWLETRPVT 204
>sp|P46429|GST2_MANSE Glutathione S-transferase 2 (GST class-sigma)
          Length = 203

 Score = 75.9 bits (185), Expect = 6e-14
 Identities = 48/165 (29%), Positives = 88/165 (53%), Gaps = 12/165 (7%)
 Frame = +3

Query: 3   PTTPTGKLPVVEYEGKKLTQSMAIARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMY 182
           P TP G++PV+E +GKK  QS+AI+R + RKYG  G D +E + I++ +  + D+     
Sbjct: 43  PNTPFGQMPVLEIDGKKYAQSLAISRYLGRKYGLAGNDIEEDFEIDQIVDFVNDIRASAA 102

Query: 183 KIYFAPEEKKEDLRTEFVTTSGRDNLKSLEGF---------IKESGFFVGEKITLAELFF 335
            + +  +   ++++ E       +N+K+   F          K +GF    ++T A+  F
Sbjct: 103 SVEYEQDAANKEVKHE-------ENMKNKYPFQLNKLSEIITKNNGFLALGRLTWADFVF 155

Query: 336 VVLTDYLEK---LPKLYDDFPKLKELRERVLKANTDVEDWVKTRP 461
           V + DYL+K   +P L + +P  K+  E VL +N  ++ ++ + P
Sbjct: 156 VGMFDYLKKMLRMPDLEEQYPIFKKPIETVL-SNPKLKAYLDSAP 199
>sp|P91254|GST8_CAEEL Probable glutathione S-transferase 8 (GST class-sigma)
          Length = 206

 Score = 74.7 bits (182), Expect = 1e-13
 Identities = 53/163 (32%), Positives = 85/163 (52%), Gaps = 8/163 (4%)
 Frame = +3

Query: 3   PTTPTGKLPVVEYEGKKLTQSMAIARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEG-- 176
           PTTP G+LP++E +GK L QS +IAR +AR++G  G+   E   +     Q  D      
Sbjct: 44  PTTPFGQLPLLEVDGKVLPQSHSIARFLARQFGINGKCAWEEAQVNSIADQFKDYRNEVR 103

Query: 177 ---MYKIYFAPEEKKEDLRTEFVTTSGRDNLKSLEGFIKE--SGFFVGEKITLAELFFVV 341
              M K+ FA E   + L  +      + +   +  F+K   SG+ VG+ +T  +L    
Sbjct: 104 PYVMVKMGFA-EGDLDALSKDVFLPGFKKHYGFIYNFLKTAGSGYLVGDSLTFVDLLIAQ 162

Query: 342 LT-DYLEKLPKLYDDFPKLKELRERVLKANTDVEDWVKTRPVT 467
            T D L   P L ++FP+ K  +E+V  +N +++ W++TRP T
Sbjct: 163 HTADILSTDPALLEEFPQFKAHQEKV-HSNANIKKWLETRPET 204
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 61,708,678
Number of Sequences: 369166
Number of extensions: 1243501
Number of successful extensions: 4195
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4054
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4162
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3502236525
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)