Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_020_I23
(954 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q13454|TUSC3_HUMAN Tumor suppressor candidate 3 (N33 pro... 363 e-100
sp|O35777|IAG2_RAT Implantation-associated protein 325 2e-88
sp|P34669|YO13_CAEEL Hypothetical protein ZK686.3 in chromo... 284 2e-76
sp|P48439|OSTG_YEAST Dolichyl-diphosphooligosaccharide--pro... 80 1e-14
sp|Q03723|OSTY_YEAST Dolichyl-diphosphooligosaccharide--pro... 50 7e-06
sp|P42377|YDNK_LACLC Hypothetical 70.0 kDa protein in dnaK ... 38 0.037
sp|P42107|YXAH_BACSU Hypothetical protein yxaH 33 1.2
sp|Q4UKU3|SYT_RICFE Threonyl-tRNA synthetase (Threonine--tR... 31 4.5
sp|Q82VV1|SYT_NITEU Threonyl-tRNA synthetase (Threonine--tR... 31 5.9
sp|Q92GE8|TRME_RICCN tRNA modification GTPase trmE 31 5.9
>sp|Q13454|TUSC3_HUMAN Tumor suppressor candidate 3 (N33 protein)
Length = 348
Score = 363 bits (933), Expect = e-100
Identities = 163/295 (55%), Positives = 225/295 (76%)
Frame = +3
Query: 3 MLGKKLTQLIDWSNRKAVIRLNTEKFKTYVKGSPKNYSMIIMLTALSPQRQCQVCKAASD 182
+L +K+ QL++WS+R+++ R+N +KF+ ++K P+NYSMI+M TAL PQRQC VC+ A++
Sbjct: 48 LLAEKVEQLMEWSSRRSIFRMNGDKFRKFIKAPPRNYSMIVMFTALQPQRQCSVCRQANE 107
Query: 183 EFSIVANSWRYTHLRNSKLFFAMVDFDDGFDVFRMLNQNTAPVFIHFPAKGNPKKADFMD 362
E+ I+ANSWRY+ +KLFF+MVD+D+G DVF+ LN N+AP F+HFP KG PK+AD D
Sbjct: 108 EYQILANSWRYSSAFCNKLFFSMVDYDEGTDVFQQLNMNSAPTFMHFPPKGRPKRADTFD 167
Query: 363 VSRSGFAAEVISKWIQDRSDIQIRVFRPPNYTGTMLLALFMSLGATVLYFKRNSLDFLKN 542
+ R GFAAE ++KWI DR+D+ IRVFRPPNY+GT+ LAL +SL +LY +RN+L+F+ N
Sbjct: 168 LQRIGFAAEQLAKWIADRTDVHIRVFRPPNYSGTIALALLVSLVGGLLYLRRNNLEFIYN 227
Query: 543 RTSWGTVSLTLIFSMMSGQMWNHIRGPPMMHANPQTGQLVFIYPSGDFQFVAETFIVLLL 722
+T W VSL ++F+M SGQMWNHIRGPP H NP GQ+ +I+ S QFVAE+ I+L+L
Sbjct: 228 KTGWAMVSLCIVFAMTSGQMWNHIRGPPYAHKNPHNGQVSYIHGSSQAQFVAESHIILVL 287
Query: 723 YIAISAGLLMMNEASFVTSDASKRKMFSLGGLALFVFFFSLAISIFRIKYQGYPY 887
AI+ G++++NEA+ D KR++ L GL L VFFFS +SIFR KY GYPY
Sbjct: 288 NAAITMGMVLLNEAATSKGDVGKRRIICLVGLGLVVFFFSFLLSIFRSKYHGYPY 342
>sp|O35777|IAG2_RAT Implantation-associated protein
Length = 335
Score = 325 bits (832), Expect = 2e-88
Identities = 149/298 (50%), Positives = 211/298 (70%)
Frame = +3
Query: 3 MLGKKLTQLIDWSNRKAVIRLNTEKFKTYVKGSPKNYSMIIMLTALSPQRQCQVCKAASD 182
+L +K+ QL++W+N++ VIR+N +KF+ VK P+NYS+I+M TAL RQC VCK A +
Sbjct: 36 VLVEKVIQLMEWTNQRPVIRMNGDKFRPLVKAPPRNYSVIVMFTALQLHRQCVVCKQADE 95
Query: 183 EFSIVANSWRYTHLRNSKLFFAMVDFDDGFDVFRMLNQNTAPVFIHFPAKGNPKKADFMD 362
EF I+AN WRY+ +++FFAMVDFD+G DVF+MLN N+AP FI+FP KG PK+AD +
Sbjct: 96 EFQILANFWRYSSAFTNRIFFAMVDFDEGSDVFQMLNMNSAPTFINFPPKGKPKRADTYE 155
Query: 363 VSRSGFAAEVISKWIQDRSDIQIRVFRPPNYTGTMLLALFMSLGATVLYFKRNSLDFLKN 542
+ GF+AE I++WI DR+D+ IRV RPPNY G ++L L +++ ++Y +R++++FL N
Sbjct: 156 LQVRGFSAEQIARWIADRTDVNIRVIRPPNYAGPLMLGLLLAVIGGLVYLRRSNMEFLFN 215
Query: 543 RTSWGTVSLTLIFSMMSGQMWNHIRGPPMMHANPQTGQLVFIYPSGDFQFVAETFIVLLL 722
+T W +L + +M SGQMWNHIRGPP H NP TG + +I+ S QFVAET IVLL
Sbjct: 216 KTGWAFAALCFVLAMTSGQMWNHIRGPPYAHKNPHTGHVNYIHGSSQAQFVAETHIVLLF 275
Query: 723 YIAISAGLLMMNEASFVTSDASKRKMFSLGGLALFVFFFSLAISIFRIKYQGYPYRLL 896
++ G++++ EA+ D KR+M + G+ L V FFS +SIFR KY GYPY L
Sbjct: 276 NGGVTLGMVLLCEAAASDMDIGKRRMMCIAGIGLVVLFFSWMLSIFRSKYHGYPYSFL 333
>sp|P34669|YO13_CAEEL Hypothetical protein ZK686.3 in chromosome III
Length = 331
Score = 284 bits (727), Expect = 2e-76
Identities = 143/316 (45%), Positives = 201/316 (63%), Gaps = 19/316 (6%)
Frame = +3
Query: 6 LGKKLTQLIDWSNRKAVIRLNTEKFKTYVKGSPKNYSMIIMLTALSPQRQCQVCKAASDE 185
L K+ L+D ++R+++++ N +K+KT V+ P+NYSMI+M TALSP QC +CK A DE
Sbjct: 14 LEDKVQNLVDLTSRQSIVKFNMDKWKTLVRMQPRNYSMIVMFTALSPGVQCPICKPAYDE 73
Query: 186 FSIVANSWRYTHLRNS--KLFFAMVDFDDGFDVFRMLNQNTAPVFIHF-PAKGNPKKADF 356
F IVANS RYT K+FF +VD++D +F+ +N NTAP+ HF P G K+ +
Sbjct: 74 FMIVANSHRYTSSEGDRRKVFFGIVDYEDAPQIFQQMNLNTAPILYHFGPKLGAKKRPEQ 133
Query: 357 MDVSRSGFAAEVISKWIQDRSDIQIRVFRPPNYTGTMLLALFMSLGATVLYFKRNSLDFL 536
MD R GF A+ I +++ D++++ +RV RPPNYT +++ALF++L +LY KRNSLDFL
Sbjct: 134 MDFQRQGFDADAIGRFVADQTEVHVRVIRPPNYTAPVVIALFVALLLGMLYMKRNSLDFL 193
Query: 537 KNRTSWGTVSLTLIFSMMSGQMWNHIRGPPMMHANPQTGQLVFIYPSGDFQFVAETFIVL 716
NRT WG V L + F MSGQMWNHIRGPP M NP T + FI+ S FQ +AET+IV
Sbjct: 194 FNRTVWGFVCLAITFIFMSGQMWNHIRGPPFMITNPNTKEPSFIHGSTQFQLIAETYIVG 253
Query: 717 LLYIAISAGLLMMNEASFVTSDASKR----------------KMFSLGGLALFVFFFSLA 848
LLY I+ G + +NEA+ ++ ++ ++ GL FFS
Sbjct: 254 LLYALIAIGFICVNEAADQSNSKDRKNAGKKLNPLSLLNIPTNTLAIAGLVCICVFFSFL 313
Query: 849 ISIFRIKYQGYPYRLL 896
+S+FR KY+GYPY L
Sbjct: 314 LSVFRSKYRGYPYSFL 329
>sp|P48439|OSTG_YEAST Dolichyl-diphosphooligosaccharide--protein glycosyltransferase gamma
subunit precursor (Oligosaccharyl transferase gamma
subunit) (Oligosaccharyl transferase 34 kDa subunit)
Length = 350
Score = 79.7 bits (195), Expect = 1e-14
Identities = 83/332 (25%), Positives = 148/332 (44%), Gaps = 30/332 (9%)
Frame = +3
Query: 3 MLGKKLTQLIDWSNRKAVIRLNTEKFKTYVKGSPKNYSMIIMLTALSPQRQCQVCKAASD 182
M +L +L + S +K +I L F+ + +N ++ + TA +P+ C +C
Sbjct: 23 MSSNRLLKLANKSPKK-IIPLKDSSFENILAPPHENAYIVALFTATAPEIGCSLCLELES 81
Query: 183 EFSIVANSWRYTHL------RNSKLFFAMVDFDDGFDV----FRMLNQNTAP-VFIHFPA 329
E+ + SW H ++ +FF V+ +D F+ N P +FI P
Sbjct: 82 EYDTIVASWFDDHPDAKSSNSDTSIFFTKVNLEDPSKTIPKAFQFFQLNNVPRLFIFKP- 140
Query: 330 KGNPKKADFMDVSRS-GFAAEVISKWIQDRSDI-QIRVFR---PPNYTGTMLLALFMSLG 494
+P D +S S +E + + IQ Q+ F P ++T + + +
Sbjct: 141 -NSPSILDHSVISISTDTGSERMKQIIQAIKQFSQVNDFSLHLPMDWTPIITSTIITFI- 198
Query: 495 ATVLYFKRNS---LDFLKNRTSWGTVSLTLIFSMMSGQMWNHIRGPPMMHANPQTGQLVF 665
TVL FK+ S + +R W T+S I M+S M+N IR + P+ + F
Sbjct: 199 -TVLLFKKQSKLMFSIISSRIIWATLSTFFIICMISAYMFNQIRNTQLAGVGPKGEVMYF 257
Query: 666 IYPSGDFQFVAETFIVLLLYIAISA-GLLMMNEASFVTSD--ASKRKMFSLGGL-----A 821
+ QF ET +++L+Y ++A ++++ F+ S +K + + + A
Sbjct: 258 LPNEFQHQFAIETQVMVLIYGTLAALVVVLVKGIQFLRSHLYPETKKAYFIDAILASFCA 317
Query: 822 LFVF-FFSLAISIFRIKYQGYPYRL--LSDPF 908
LF++ FF+ ++F IK YP+ L LS PF
Sbjct: 318 LFIYVFFAALTTVFTIKSPAYPFPLLRLSAPF 349
>sp|Q03723|OSTY_YEAST Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 37
kDa subunit (Oligosaccharyl transferase 37 kDa subunit)
(OTase 37 kDa subunit)
Length = 332
Score = 50.4 bits (119), Expect = 7e-06
Identities = 60/302 (19%), Positives = 127/302 (42%), Gaps = 13/302 (4%)
Frame = +3
Query: 18 LTQLIDWSNRKAVIRLNTEKFKTYVKGSPKNYSM--IIMLTALSPQRQCQVCKAASDEFS 191
+ ++ + VI + + + +G P +++ I M S CQ+C +
Sbjct: 30 IDDILQLKDDTGVITVTADNYPLLSRGVPGYFNILYITMRGTNSNGMSCQLCHDFEKTYH 89
Query: 192 IVANSWRYTHLRNSKLFFAMVDFDDGFDVFRMLNQNTAPVFIHFPAKGNPKKADFMDVSR 371
VA+ R ++ LFF VD ++ + + L P + +P + K++ F +
Sbjct: 90 AVADVIRSQAPQSLNLFFT-VDVNEVPQLVKDLKLQNVPHLVVYPPAESNKQSQFEWKTS 148
Query: 372 SGFAAEVISKWIQDRSD--------IQIRVFRPPNYTGTMLLALFMSLGATVLYFKRNSL 527
+ ++ + ++ + I + P + + F++ ++ K+ L
Sbjct: 149 PFYQYSLVPENAENTLQFGDFLAKILNISITVPQAFNVQEFVYYFVACMVVFIFIKKVIL 208
Query: 528 DFLKNRTSWGTV--SLTLIFSMMSGQMWNHIRGPPMMHANPQTGQLVFIYPSGDFQFVAE 701
+ N+ ++ SL ++ ++G + + P + + + + F SG +QF E
Sbjct: 209 PKVTNKWKLFSMILSLGILLPSITGYKFVEMNAIPFIARDAKNRIMYFSGGSG-WQFGIE 267
Query: 702 TFIVLLLYIAISAGLLMMNEASFVTSDASKRKMFSLGGLALFVF-FFSLAISIFRIKYQG 878
F V L+YI +SA +++ ++ + K + LA +F FFS IS + IK G
Sbjct: 268 IFSVSLMYIVMSALSVLLIYVPKISCVSEKMRGLLSSFLACVLFYFFSYFISCYLIKNPG 327
Query: 879 YP 884
YP
Sbjct: 328 YP 329
>sp|P42377|YDNK_LACLC Hypothetical 70.0 kDa protein in dnaK 3'region (ORF4)
Length = 614
Score = 38.1 bits (87), Expect = 0.037
Identities = 30/101 (29%), Positives = 44/101 (43%)
Frame = +3
Query: 594 GQMWNHIRGPPMMHANPQTGQLVFIYPSGDFQFVAETFIVLLLYIAISAGLLMMNEASFV 773
G WN GP + + FI S F F L L+I+ G+ + N +SF+
Sbjct: 79 GYAWNEFYGPLPTYF---ISVIKFIVKSWSLSF--SLFYSLCLFIS---GIFIFNFSSFL 130
Query: 774 TSDASKRKMFSLGGLALFVFFFSLAISIFRIKYQGYPYRLL 896
D + K+F L +ALF F S I+++ P LL
Sbjct: 131 LKDHTNSKLFGLLAVALFTFSNSTYINLYYYANPSQPLALL 171
>sp|P42107|YXAH_BACSU Hypothetical protein yxaH
Length = 402
Score = 33.1 bits (74), Expect = 1.2
Identities = 34/127 (26%), Positives = 52/127 (40%), Gaps = 9/127 (7%)
Frame = +3
Query: 468 LLALFMSLGATVLYFKRNSLDFLKNRTSWGTVSLTLIFSM---------MSGQMWNHIRG 620
+L + LG +L F++ K W VSL L+FS+ +GQ W
Sbjct: 127 ILTEYALLGFVLLLFRKAKP---KTLLIWA-VSLYLLFSIPFMLTSFDQSNGQEW----- 177
Query: 621 PPMMHANPQTGQLVFIYPSGDFQFVAETFIVLLLYIAISAGLLMMNEASFVTSDASKRKM 800
+ Q Q + +Y SG + +AE I L S G+L +N ++ + M
Sbjct: 178 --VQAVTQQAKQAIHVYGSGSLKDIAEQRIHDRLVYMSSNGMLRINPLNYFFASIPYFSM 235
Query: 801 FSLGGLA 821
F LG A
Sbjct: 236 FLLGAAA 242
>sp|Q4UKU3|SYT_RICFE Threonyl-tRNA synthetase (Threonine--tRNA ligase) (ThrRS)
Length = 635
Score = 31.2 bits (69), Expect = 4.5
Identities = 15/46 (32%), Positives = 26/46 (56%)
Frame = +2
Query: 455 HRNDAAGVIHVAGRHRALLQAEQFGFLEEQDVLGHSFADAHLLDDV 592
HRN+A+G +H R R+L+Q + F E+ + + + LL +V
Sbjct: 361 HRNEASGALHGLMRVRSLVQDDAHIFCAEEQITDETVSFCKLLTEV 406
>sp|Q82VV1|SYT_NITEU Threonyl-tRNA synthetase (Threonine--tRNA ligase) (ThrRS)
Length = 635
Score = 30.8 bits (68), Expect = 5.9
Identities = 17/49 (34%), Positives = 24/49 (48%)
Frame = +2
Query: 455 HRNDAAGVIHVAGRHRALLQAEQFGFLEEQDVLGHSFADAHLLDDVGAN 601
HRN+A+G +H R R+ Q + F E +LG LL+ V N
Sbjct: 361 HRNEASGALHGLMRVRSFTQDDAHIFCTEDQILGEVTKFIDLLNQVYIN 409
>sp|Q92GE8|TRME_RICCN tRNA modification GTPase trmE
Length = 445
Score = 30.8 bits (68), Expect = 5.9
Identities = 28/122 (22%), Positives = 56/122 (45%), Gaps = 2/122 (1%)
Frame = +3
Query: 126 MLTALSPQRQCQVCKAASDEFSIVANSWRYTHLRNSKLFFAMVDFDDGFDVFRMLNQ--N 299
++ A + + Q + AS + + N+WR L+ L A +DF D +LN+ N
Sbjct: 131 LINAETIMQHKQAIRQASGKLEALYNNWRSQLLKMLSLLEAYIDFPDEDIPDTVLNEVTN 190
Query: 300 TAPVFIHFPAKGNPKKADFMDVSRSGFAAEVISKWIQDRSDIQIRVFRPPNYTGTMLLAL 479
T + ++ +++++ +R G E++ RS +++ + PPN + LL
Sbjct: 191 THTILVN-------TISEYLNDNRKG---ELL------RSGLKLAIIGPPNVGKSSLLNF 234
Query: 480 FM 485
M
Sbjct: 235 LM 236
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 119,622,843
Number of Sequences: 369166
Number of extensions: 2661481
Number of successful extensions: 6838
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6516
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6828
length of database: 68,354,980
effective HSP length: 111
effective length of database: 47,849,395
effective search space used: 9856975370
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)