Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Dr_sW_020_I21 (135 letters) Database: Non-redundant SwissProt sequences 184,735 sequences; 68,354,980 total letters Score E Sequences producing significant alignments: (bits) Value sp|P46467|VPS4B_MOUSE Vacuolar sorting protein 4b (SKD1 pro... 34 0.091 sp|O75351|VPS4B_HUMAN Vacuolar sorting protein 4b (SKD1 pro... 33 0.15 sp|P52917|VPS4_YEAST Vacuolar protein sorting-associated pr... 32 0.59 sp|Q09803|VPS4_SCHPO Suppressor protein of bem1/bed5 double... 31 1.0 sp|Q9UN37|VPS4A_HUMAN Vacuolar sorting protein 4a (SKD2 pro... 28 5.0 sp|Q8VEJ9|VPS4A_MOUSE Vacuolar sorting protein 4a 28 5.0
>sp|P46467|VPS4B_MOUSE Vacuolar sorting protein 4b (SKD1 protein) Length = 444 Score = 34.3 bits (77), Expect = 0.091 Identities = 15/21 (71%), Positives = 18/21 (85%) Frame = +1 Query: 1 KPTVNQKDLEKLIQFTTDFGQ 63 KPTVN++DL KL +FT DFGQ Sbjct: 422 KPTVNEQDLLKLKKFTEDFGQ 442
>sp|O75351|VPS4B_HUMAN Vacuolar sorting protein 4b (SKD1 protein) Length = 444 Score = 33.5 bits (75), Expect = 0.15 Identities = 15/21 (71%), Positives = 17/21 (80%) Frame = +1 Query: 1 KPTVNQKDLEKLIQFTTDFGQ 63 KPTVN+ DL KL +FT DFGQ Sbjct: 422 KPTVNEHDLLKLKKFTEDFGQ 442
>sp|P52917|VPS4_YEAST Vacuolar protein sorting-associated protein VPS4 (END13 protein) (DOA4-independent degradation protein 6) Length = 437 Score = 31.6 bits (70), Expect = 0.59 Identities = 14/21 (66%), Positives = 16/21 (76%) Frame = +1 Query: 1 KPTVNQKDLEKLIQFTTDFGQ 63 +PTVN+ DL K QFT DFGQ Sbjct: 414 RPTVNEDDLLKQEQFTRDFGQ 434
>sp|Q09803|VPS4_SCHPO Suppressor protein of bem1/bed5 double mutants Length = 432 Score = 30.8 bits (68), Expect = 1.0 Identities = 13/20 (65%), Positives = 15/20 (75%) Frame = +1 Query: 1 KPTVNQKDLEKLIQFTTDFG 60 KPT+N D+EK QFT DFG Sbjct: 410 KPTLNAGDIEKHTQFTKDFG 429
>sp|Q9UN37|VPS4A_HUMAN Vacuolar sorting protein 4a (SKD2 protein) (hVPS4) (VPS4-1) Length = 437 Score = 28.5 bits (62), Expect = 5.0 Identities = 12/21 (57%), Positives = 16/21 (76%) Frame = +1 Query: 1 KPTVNQKDLEKLIQFTTDFGQ 63 +PTVN DL K+ +F+ DFGQ Sbjct: 415 RPTVNADDLLKVKKFSEDFGQ 435
>sp|Q8VEJ9|VPS4A_MOUSE Vacuolar sorting protein 4a Length = 437 Score = 28.5 bits (62), Expect = 5.0 Identities = 12/21 (57%), Positives = 16/21 (76%) Frame = +1 Query: 1 KPTVNQKDLEKLIQFTTDFGQ 63 +PTVN DL K+ +F+ DFGQ Sbjct: 415 RPTVNADDLLKVKKFSEDFGQ 435
Database: Non-redundant SwissProt sequences Posted date: Dec 6, 2005 7:40 AM Number of letters in database: 68,354,980 Number of sequences in database: 184,735 Database: swissprot.01 Posted date: Dec 6, 2005 8:18 AM Number of letters in database: 66,202,850 Number of sequences in database: 184,431 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,954,669 Number of Sequences: 369166 Number of extensions: 110147 Number of successful extensions: 185 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 185 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 185 length of database: 68,354,980 effective HSP length: 18 effective length of database: 65,029,750 effective search space used: 1690773500 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)