Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_020_I04
(926 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9M8D3|PUR4_ARATH Probable phosphoribosylformylglycinami... 35 0.30
sp|Q8A2A1|IF2_BACTN Translation initiation factor IF-2 35 0.39
sp|P48415|SEC16_YEAST Multidomain vesicle coat protein 34 0.51
sp|O60237|MYPT2_HUMAN Protein phosphatase 1 regulatory subu... 34 0.67
sp|P53210|YG1B_YEAST Hypothetical 34.7 kDa protein in MSB2-... 33 0.87
sp|Q98PF2|SYN_MYCPU Asparaginyl-tRNA synthetase (Asparagine... 33 1.5
sp|P30419|NMT1_HUMAN Glycylpeptide N-tetradecanoyltransfera... 32 2.5
sp|Q5R741|ZN394_PONPY Zinc finger protein 394 32 2.5
sp|Q00383|YCF1_EPIVI Hypothetical 208 kDa protein ycf1 (ORF... 32 3.3
sp|Q6DBP8|GATA6_ARATH GATA transcription factor 6 (AtGATA-6) 31 4.3
>sp|Q9M8D3|PUR4_ARATH Probable phosphoribosylformylglycinamidine synthase, chloroplast
precursor (FGAM synthase) (FGAMS) (Formylglycinamide
ribotide amidotransferase) (FGARAT) (Formylglycinamide
ribotide synthetase)
Length = 1387
Score = 35.0 bits (79), Expect = 0.30
Identities = 21/52 (40%), Positives = 26/52 (50%), Gaps = 9/52 (17%)
Frame = +2
Query: 500 YCDDDRSSNQTSSH------QPPNRAATASGDRCH---NPHPSKAFLVWRFP 628
YCDDD N T ++ P AA S D H PHP + FL+W+FP
Sbjct: 1308 YCDDD--GNVTEAYPFNLNGSPLGIAAICSPDGRHLAMMPHPERCFLMWQFP 1357
>sp|Q8A2A1|IF2_BACTN Translation initiation factor IF-2
Length = 1040
Score = 34.7 bits (78), Expect = 0.39
Identities = 35/137 (25%), Positives = 63/137 (45%), Gaps = 9/137 (6%)
Frame = +2
Query: 68 IIVAKSIIKPGLKNSLENFILQTNKAKDREKALEILNSIEDYNKRFNRNNNLVKEYKLKT 247
++V + L+ E FI Q + K+R KA SIE + K+ + + E +KT
Sbjct: 45 VLVKEFSTDKNLRLESERFI-QERQNKERNKASV---SIEGFEKQQEKPKS---EDVIKT 97
Query: 248 ALQRPKTAGSRFK-----DGPFIHQYSTTYTEDFPKKKLE----ETIANRPASTENLISR 400
+ P+ A +FK D T E P+KK+E + + +P + + ++ +
Sbjct: 98 VV--PEDARPKFKPVGKIDLDKFSGRRTDKVEKAPEKKVEPVVEQPVVEQPVAEKTVVEK 155
Query: 401 PISSTEIKSEMKNREKL 451
P+ +E+K E EK+
Sbjct: 156 PVVESEVKKEEPKVEKV 172
>sp|P48415|SEC16_YEAST Multidomain vesicle coat protein
Length = 2195
Score = 34.3 bits (77), Expect = 0.51
Identities = 57/246 (23%), Positives = 101/246 (41%), Gaps = 25/246 (10%)
Frame = +2
Query: 125 ILQTN---KAKDREKALEILNSIEDYNKRFNRNNNLVKEYKLKTALQRPKTAGSRFKDGP 295
ILQTN + D K L SIE+ + N+ K ++ P T G ++ P
Sbjct: 1742 ILQTNVEVRGTDASKMENSLPSIENERSSEEQPENISKS---ASSAYLPSTGGLSLENRP 1798
Query: 296 FIHQYSTTYTEDFPKKKLEETIANR--PASTENLISRPISSTE-------------IKSE 430
T+D + + ET+ + PA + ++ ++PIS + ++
Sbjct: 1799 L--------TQD--ENSISETVQSTYLPAGSISMEAKPISQVQDVPRNVNNKASKLVEQH 1848
Query: 431 M-----KNREKLFKSYSTYDPRSTYTNDYCDDDRSSNQTSSHQPPNRAATASGDRCHNPH 595
M K+ + +YS Y P+ST + D D S+ +S A A + H +
Sbjct: 1849 MAPPKPKSTDATKMNYSPYVPQSTAAS--ADGDESTILKTS-----PAIYARTHQAHASN 1901
Query: 596 PSKAFLVWRFpreSVNKETRVPSETALKINMENNFAQINDDQFNRVMRGNC--KSTYQAD 769
PS+ F + E+ + E SE+ + N A ++++F+ + + K T+Q
Sbjct: 1902 PSQYFPLVNQANETASFEL---SESTSQAQSNGNVA--SENRFSPIKKAEVVEKDTFQPT 1956
Query: 770 FRGGST 787
R ST
Sbjct: 1957 IRKAST 1962
>sp|O60237|MYPT2_HUMAN Protein phosphatase 1 regulatory subunit 12B (Myosin phosphatase
targeting subunit 2) (Myosin phosphatase target subunit
2)
Length = 982
Score = 33.9 bits (76), Expect = 0.67
Identities = 31/112 (27%), Positives = 46/112 (41%), Gaps = 16/112 (14%)
Frame = +2
Query: 128 LQTNKAKDREKALEI-------LNSIEDYNKRFNRNN--NLVKEYKLKTALQRPKTAGSR 280
L NK K+RE I LNS D ++ NR + NLV+ L R + G+
Sbjct: 480 LLDNKDKERENKSYISSLAPRKLNSTSDIEEKENRESAVNLVRSGSYTRQLWRDEAKGNE 539
Query: 281 FKDGPFIHQYSTTYTEDFPKKKLEETIANRPASTEN-------LISRPISST 415
Y +TY + P K +T A + A + + +RP+ ST
Sbjct: 540 IPQTIAPSTYVSTYLKRTPHKSQADTTAEKTADNVSSSTPLCVITNRPLPST 591
>sp|P53210|YG1B_YEAST Hypothetical 34.7 kDa protein in MSB2-UGA1 intergenic region
Length = 297
Score = 33.5 bits (75), Expect = 0.87
Identities = 30/125 (24%), Positives = 59/125 (47%), Gaps = 11/125 (8%)
Frame = +2
Query: 377 STENLISRPISST-EIKSEMKNREKLFKSYSTYDPRSTYTNDYCDDDRSSNQTSSHQPPN 553
+TE +S ++ E+ ++ + R +F+ + +D R+ + D RSS T S PN
Sbjct: 19 NTEPFVSFQFATVDELTNKPRCRTVVFRDFLFHDKRTNVLT-FNTDMRSSKITESFITPN 77
Query: 554 RAATASGDRCHNPHPSKAFLV------WRFpre--SVNKE-TRVPSETALKINM-ENNFA 703
++ RC P F +RF + +++K+ ++P+E K ++ F+
Sbjct: 78 SNNSSDSKRCETPFFEACFYFPETWEQYRFSGQCFTISKQFKKIPAEIVTKYDIFSPRFS 137
Query: 704 QINDD 718
+ NDD
Sbjct: 138 ETNDD 142
>sp|Q98PF2|SYN_MYCPU Asparaginyl-tRNA synthetase (Asparagine--tRNA ligase) (AsnRS)
Length = 450
Score = 32.7 bits (73), Expect = 1.5
Identities = 22/65 (33%), Positives = 33/65 (50%)
Frame = +2
Query: 65 EIIVAKSIIKPGLKNSLENFILQTNKAKDREKALEILNSIEDYNKRFNRNNNLVKEYKLK 244
E++ + IKPGL SLE+F+ + + K +EIL + NK F N+ LK
Sbjct: 267 ELVFLDTHIKPGLIQSLEDFLESNLEIIEYRKVIEIL----EKNKVFFEEQNIEFGMDLK 322
Query: 245 TALQR 259
T +R
Sbjct: 323 TEHER 327
>sp|P30419|NMT1_HUMAN Glycylpeptide N-tetradecanoyltransferase 1 (Peptide
N-myristoyltransferase 1) (Myristoyl-CoA:protein
N-myristoyltransferase 1) (NMT 1) (Type I
N-myristoyltransferase)
Length = 496
Score = 32.0 bits (71), Expect = 2.5
Identities = 20/54 (37%), Positives = 26/54 (48%), Gaps = 3/54 (5%)
Frame = +2
Query: 182 IEDYNKRFNRNNNLVKEYKLKTALQRPKTAGSR---FKDGPFIHQYSTTYTEDF 334
IE +RN + + KL + PKTAG R KD P +HQ T Y + F
Sbjct: 307 IEVKFSHLSRNMTMQRTMKLYRLPETPKTAGLRPMETKDIPVVHQLLTRYLKQF 360
>sp|Q5R741|ZN394_PONPY Zinc finger protein 394
Length = 574
Score = 32.0 bits (71), Expect = 2.5
Identities = 28/118 (23%), Positives = 46/118 (38%), Gaps = 11/118 (9%)
Frame = +2
Query: 185 EDYNKRFNRNNNLVKEYKLKTALQRPKTA---GSRFKDGPFIHQYSTTYTEDFPKKKLEE 355
++ K F+++ L K + T ++P T G RF+ ++++ +T++ D K EE
Sbjct: 389 QECGKSFSQSAALTKHQRTHTG-EKPYTCLKCGERFRQNSHLNRHQSTHSRD-KHFKCEE 446
Query: 356 TIANRPASTENLI--------SRPISSTEIKSEMKNREKLFKSYSTYDPRSTYTNDYC 505
NL RP E K K R LFK + + Y C
Sbjct: 447 --CGETCRISNLFRHQRLHKGERPYKCEECKKSFKQRSDLFKHHRIHTGEKPYGCSVC 502
>sp|Q00383|YCF1_EPIVI Hypothetical 208 kDa protein ycf1 (ORF 1738)
Length = 1738
Score = 31.6 bits (70), Expect = 3.3
Identities = 21/63 (33%), Positives = 28/63 (44%), Gaps = 11/63 (17%)
Frame = +2
Query: 89 IKPGLKNSLENFILQTNKAKDREKALEILNSIEDYNKRFNR-----------NNNLVKEY 235
++ L N L NF L TNK + E +N IE +K+F N+N KEY
Sbjct: 439 LEKALTNKLSNFWLYTNKKSIKNFTDEFINRIEALDKKFISYNILETRTRLCNDNYTKEY 498
Query: 236 KLK 244
K
Sbjct: 499 LSK 501
>sp|Q6DBP8|GATA6_ARATH GATA transcription factor 6 (AtGATA-6)
Length = 303
Score = 31.2 bits (69), Expect = 4.3
Identities = 26/101 (25%), Positives = 38/101 (37%)
Frame = +2
Query: 365 NRPASTENLISRPISSTEIKSEMKNREKLFKSYSTYDPRSTYTNDYCDDDRSSNQTSSHQ 544
N PA ++ S +SST +S K+ K + + P S N Y S T ++
Sbjct: 85 NIPALKQSYSSEALSSTLHQSSAPPEIKVSKLFQSLSPVSVLENSY-----GSLSTHNNG 139
Query: 545 PPNRAATASGDRCHNPHPSKAFLVWRFpreSVNKETRVPSE 667
A G R P+ L + FP E E P +
Sbjct: 140 SQRLAFPVKGMRSKRKRPTTLRLSYLFPSEPRKPEKSTPGK 180
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 99,238,233
Number of Sequences: 369166
Number of extensions: 1912742
Number of successful extensions: 6147
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5888
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6137
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9510757740
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)