Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_020_I03
(521 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P37167|ACTP_ACACA Actophorin 72 8e-13
sp|P54706|COFI_DICDI Cofilin 62 1e-09
sp|P78929|COFI_SCHPO Cofilin 61 1e-09
sp|P45593|COF2_XENLA Cofilin-2 61 2e-09
sp|Q9ZSK3|ADF4_ARATH Actin-depolymerizing factor 4 (ADF-4) ... 60 4e-09
sp|Q03048|COFI_YEAST Cofilin 59 5e-09
sp|Q9LQ81|ADFX_ARATH Actin-depolymerizing factor like At1g0... 59 5e-09
sp|Q9Y281|COF2_HUMAN Cofilin-2 (Cofilin, muscle isoform) 59 9e-09
sp|P45695|COF1_XENLA Cofilin-1 59 9e-09
sp|P45591|COF2_MOUSE Cofilin-2 (Cofilin, muscle isoform) 59 9e-09
>sp|P37167|ACTP_ACACA Actophorin
Length = 138
Score = 72.0 bits (175), Expect = 8e-13
Identities = 37/139 (26%), Positives = 66/139 (47%), Gaps = 8/139 (5%)
Frame = +3
Query: 21 SGVKCDPSVEEYLNYIKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYA 200
SG+ + N +KL H+ +YV + +N ++ G YE FK + E
Sbjct: 2 SGIAVSDDCVQKFNELKLGHQHRYVTFKMNASNTEVVVEHVGGPNATYEDFKSQLPERDC 61
Query: 201 QEGCYALYDYE--------GTLAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEA 356
+ YA++DYE + FI W P++ I S+M+ ++K I ++VG++ +++A
Sbjct: 62 R---YAIFDYEFQVDGGQRNKITFILWAPDSAPIKSKMMYTSTKDSIKKKLVGIQVEVQA 118
Query: 357 NSEDEIDESVIKEKTASKV 413
EI E + E+ V
Sbjct: 119 TDAAEISEDAVSERAKKDV 137
>sp|P54706|COFI_DICDI Cofilin
Length = 137
Score = 61.6 bits (148), Expect = 1e-09
Identities = 34/136 (25%), Positives = 65/136 (47%), Gaps = 8/136 (5%)
Frame = +3
Query: 15 MASGVKCDPSVEEYLNYIKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIET 194
M+SG+ P+ N +KL K+ +IY ++ + II T G +++F + + E
Sbjct: 1 MSSGIALAPNCVSTFNDLKLGRKYGGIIYRISDDSKEIIVDSTLPAGCSFDEFTKCLPEN 60
Query: 195 YAQEGCYALYDYE--------GTLAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKI 350
+ Y + DY+ + F++W P+T I +M+ +SK + VG++ +I
Sbjct: 61 ECR---YVVLDYQYKEEGAQKSKICFVAWCPDTANIKKKMMATSSKDSLRKACVGIQVEI 117
Query: 351 EANSEDEIDESVIKEK 398
+ E+ +S EK
Sbjct: 118 QGTDASEVKDSCFYEK 133
>sp|P78929|COFI_SCHPO Cofilin
Length = 137
Score = 61.2 bits (147), Expect = 1e-09
Identities = 39/138 (28%), Positives = 62/138 (44%), Gaps = 8/138 (5%)
Frame = +3
Query: 21 SGVKCDPSVEEYLNYIKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYA 200
SGVK P E +KL +YV++ +N K I+ +E ++ F + E
Sbjct: 4 SGVKVSPECLEAFQELKLGKSLRYVVFKMNDTKTEIV-VEKKSTDKDFDTFLGDLPEKDC 62
Query: 201 QEGCYALYDYEGTLA--------FISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEA 356
+ YA+YD+E L FISW P+ I S+M+ ++SK + G+ I+A
Sbjct: 63 R---YAIYDFEFNLGEGVRNKIIFISWSPDVAPIKSKMVYSSSKDTLRRAFTGIGTDIQA 119
Query: 357 NSEDEIDESVIKEKTASK 410
E+ + EK K
Sbjct: 120 TDFSEVAYETVLEKVTRK 137
>sp|P45593|COF2_XENLA Cofilin-2
Length = 168
Score = 60.8 bits (146), Expect = 2e-09
Identities = 45/145 (31%), Positives = 72/145 (49%), Gaps = 23/145 (15%)
Frame = +3
Query: 15 MASGVKCDPSVEEYLNYIKLQHKF---------QYVIYGLNKEKNRII-----KLETGDL 152
MASGV V + N +K++H+ + VI+ L+ +K II ++ GD+
Sbjct: 1 MASGVMVSDDVVKVFNDMKVRHQLSPEEAKKRKKAVIFCLSDDKKTIILEPGKEILQGDV 60
Query: 153 GTPYEKFKQHIIETYAQEGC-YALYD--YEGT------LAFISWIPETMKINSRMIMAAS 305
G E + ++ + C YALYD YE L F+ W PE + S+MI A+S
Sbjct: 61 GCNVEDPYKTFVKMLPRNDCRYALYDALYETKETKKEDLVFVFWAPEEASLKSKMIYASS 120
Query: 306 KSEIATRMVGVKAKIEANSEDEIDE 380
K I R G+K + + N+ D+I++
Sbjct: 121 KDAIRKRFTGIKHEWQTNTYDDIND 145
>sp|Q9ZSK3|ADF4_ARATH Actin-depolymerizing factor 4 (ADF-4) (AtADF4)
Length = 139
Score = 59.7 bits (143), Expect = 4e-09
Identities = 34/136 (25%), Positives = 68/136 (50%), Gaps = 9/136 (6%)
Frame = +3
Query: 18 ASGVKCDPSVEEYLNYIKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETY 197
ASG+ + +K + ++++Y + +++ ++I + G+ YE F +
Sbjct: 5 ASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVIVEKVGEPILTYEDFAASL---P 61
Query: 198 AQEGCYALYDYEGTLA---------FISWIPETMKINSRMIMAASKSEIATRMVGVKAKI 350
A E YA+YD++ A FI+W P+ K+ S+MI A+SK + G++ ++
Sbjct: 62 ADECRYAIYDFDFVTAENCQKSKIFFIAWCPDVAKVRSKMIYASSKDRFKRELDGIQVEL 121
Query: 351 EANSEDEIDESVIKEK 398
+A E+D V+K +
Sbjct: 122 QATDPTEMDLDVLKSR 137
>sp|Q03048|COFI_YEAST Cofilin
Length = 143
Score = 59.3 bits (142), Expect = 5e-09
Identities = 35/138 (25%), Positives = 64/138 (46%), Gaps = 10/138 (7%)
Frame = +3
Query: 21 SGVKCDPSVEEYLNYIKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYA 200
SGV N +KL K++++++GLN K I+ ET Y+ F + + E
Sbjct: 4 SGVAVADESLTAFNDLKLGKKYKFILFGLNDAKTEIVVKETST-DPSYDAFLEKLPEN-- 60
Query: 201 QEGCYALYDYE----------GTLAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKI 350
+ YA+YD+E + F +W P+T + S+M+ A+SK + + GV +
Sbjct: 61 -DCLYAIYDFEYEINGNEGKRSKIVFFTWSPDTAPVRSKMVYASSKDALRRALNGVSTDV 119
Query: 351 EANSEDEIDESVIKEKTA 404
+ E+ + E+ +
Sbjct: 120 QGTDFSEVSYDSVLERVS 137
>sp|Q9LQ81|ADFX_ARATH Actin-depolymerizing factor like At1g01750 (ADF-like)
Length = 140
Score = 59.3 bits (142), Expect = 5e-09
Identities = 32/134 (23%), Positives = 70/134 (52%), Gaps = 9/134 (6%)
Frame = +3
Query: 18 ASGVKCDPSVEEYLNYIKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETY 197
ASG+ + +K + ++++++ ++++ +++ + G+ YE F + I E
Sbjct: 5 ASGMHVSDECKLKFLELKAKRNYRFIVFKIDEKAQQVMIDKLGNPEETYEDFTRSIPE-- 62
Query: 198 AQEGCYALYDYEGT---------LAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKI 350
E YA+YDY+ T + FI+W P+T ++ S+M+ A+SK + G++ ++
Sbjct: 63 -DECRYAVYDYDFTTPENCQKSKIFFIAWSPDTSRVRSKMLYASSKDRFKRELDGIQVEL 121
Query: 351 EANSEDEIDESVIK 392
+A E+ +IK
Sbjct: 122 QATDPSEMSLDIIK 135
>sp|Q9Y281|COF2_HUMAN Cofilin-2 (Cofilin, muscle isoform)
Length = 166
Score = 58.5 bits (140), Expect = 9e-09
Identities = 49/157 (31%), Positives = 74/157 (47%), Gaps = 24/157 (15%)
Frame = +3
Query: 15 MASGVKCDPSVEEYLNYIK---------LQHKFQYVIYGLNKEKNRIIKLET-----GDL 152
MASGV + V + N +K ++ + + V++ L+ +K +II E GD+
Sbjct: 1 MASGVTVNDEVIKVFNDMKVRKSSTQEEIKKRKKAVLFCLSDDKRQIIVEEAKQILVGDI 60
Query: 153 GTPYEKFKQHIIETYAQEGC-YALYD--YEGT------LAFISWIPETMKINSRMIMAAS 305
G E ++ C YALYD YE L FI W PE+ + S+MI A+S
Sbjct: 61 GDTVEDPYTSFVKLLPLNDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASS 120
Query: 306 KSEIATRMVGVKAKIEANSEDEI-DESVIKEKTASKV 413
K I + G+K + + N D+I D S + EK V
Sbjct: 121 KDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKLGGNV 157
>sp|P45695|COF1_XENLA Cofilin-1
Length = 168
Score = 58.5 bits (140), Expect = 9e-09
Identities = 42/145 (28%), Positives = 73/145 (50%), Gaps = 23/145 (15%)
Frame = +3
Query: 15 MASGVKCDPSVEEYLNYIKLQHKF---------QYVIYGLNKEKNRII-----KLETGDL 152
MASGV V + N +K++H+ + V++ L+ +K II ++ GD+
Sbjct: 1 MASGVMVSDDVIKVFNEMKVRHQLSPEDAKKRKKAVVFCLSDDKKTIILEPGKEILQGDI 60
Query: 153 GTPYEKFKQHIIETYAQEGC-YALYD--YEGT------LAFISWIPETMKINSRMIMAAS 305
G E + ++ + C YALYD YE L F+ W PE + S+MI A+S
Sbjct: 61 GCNVEDPYKTFVKMLPRNDCRYALYDALYETKETKKEDLVFVFWAPEEASLKSKMIYASS 120
Query: 306 KSEIATRMVGVKAKIEANSEDEIDE 380
K I R+ G+K + + N+ +++++
Sbjct: 121 KDAIKKRLPGIKHEWQINTYEDVND 145
>sp|P45591|COF2_MOUSE Cofilin-2 (Cofilin, muscle isoform)
Length = 166
Score = 58.5 bits (140), Expect = 9e-09
Identities = 49/157 (31%), Positives = 74/157 (47%), Gaps = 24/157 (15%)
Frame = +3
Query: 15 MASGVKCDPSVEEYLNYIK---------LQHKFQYVIYGLNKEKNRIIKLET-----GDL 152
MASGV + V + N +K ++ + + V++ L+ +K +II E GD+
Sbjct: 1 MASGVTVNDEVIKVFNDMKVRKSSTQEEIKKRKKAVLFCLSDDKRQIIVEEAKQILVGDI 60
Query: 153 GTPYEKFKQHIIETYAQEGC-YALYD--YEGT------LAFISWIPETMKINSRMIMAAS 305
G E ++ C YALYD YE L FI W PE+ + S+MI A+S
Sbjct: 61 GDTVEDPYTSFVKLLPLNDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASS 120
Query: 306 KSEIATRMVGVKAKIEANSEDEI-DESVIKEKTASKV 413
K I + G+K + + N D+I D S + EK V
Sbjct: 121 KDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKLGGSV 157
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 51,187,500
Number of Sequences: 369166
Number of extensions: 863986
Number of successful extensions: 2059
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2003
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2046
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3452909250
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)