Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_020_G09
(394 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P19623|SPEE_HUMAN Spermidine synthase (Putrescine aminop... 84 1e-16
sp|Q64674|SPEE_MOUSE Spermidine synthase (Putrescine aminop... 84 1e-16
sp|Q09741|SPEE_SCHPO Spermidine synthase (Putrescine aminop... 79 3e-15
sp|Q9Y8H7|SPEE_NEUCR Spermidine synthase (Putrescine aminop... 76 3e-14
sp|Q9SMB1|SPD1_ORYSA Spermidine synthase 1 (Putrescine amin... 75 5e-14
sp|Q12074|SPEE_YEAST Spermidine synthase (Putrescine aminop... 74 1e-13
sp|O48661|SPD2_ARATH Spermidine synthase 2 (Putrescine amin... 74 1e-13
sp|Q12455|SPSY_YEAST Spermine synthase (Spermidine aminopro... 72 3e-13
sp|Q9ZUB3|SPD1_ARATH Spermidine synthase 1 (Putrescine amin... 72 5e-13
sp|O48659|SPD2_HYONI Spermidine synthase 2 (Putrescine amin... 71 9e-13
>sp|P19623|SPEE_HUMAN Spermidine synthase (Putrescine aminopropyltransferase) (SPDSY)
Length = 302
Score = 84.0 bits (206), Expect = 1e-16
Identities = 39/92 (42%), Positives = 56/92 (60%), Gaps = 3/92 (3%)
Frame = +2
Query: 2 ESIWFENKIVSDLVDKAKAVFPNVKYASGCVPTYPLGQIGYLLCSKNPDTNFS---KPLF 172
E W ++ ++ +++FP V YA +PTYP GQIG++LCSKNP TNF +PL
Sbjct: 208 ECQWLHLDLIKEMRQFCQSLFPVVAYAYCTIPTYPSGQIGFMLCSKNPSTNFQEPVQPLT 267
Query: 173 DVSTIENQFKYYSSDIHTASFVLPNCMKHIIN 268
+ Q KYY+SD+H A+FVLP + +N
Sbjct: 268 QQQVAQMQLKYYNSDVHRAAFVLPEFARKALN 299
>sp|Q64674|SPEE_MOUSE Spermidine synthase (Putrescine aminopropyltransferase) (SPDSY)
Length = 302
Score = 84.0 bits (206), Expect = 1e-16
Identities = 40/92 (43%), Positives = 56/92 (60%), Gaps = 3/92 (3%)
Frame = +2
Query: 2 ESIWFENKIVSDLVDKAKAVFPNVKYASGCVPTYPLGQIGYLLCSKNPDTNFSKP---LF 172
E W ++ ++ K++FP V YA +PTYP GQIG++LCSKNP TNF +P L
Sbjct: 208 ECQWLHLDLIKEMRHFCKSLFPVVDYAYCSIPTYPSGQIGFMLCSKNPSTNFREPVQQLT 267
Query: 173 DVSTIENQFKYYSSDIHTASFVLPNCMKHIIN 268
+ Q KYY+SD+H A+FVLP + +N
Sbjct: 268 QAQVEQMQLKYYNSDMHRAAFVLPEFTRKALN 299
>sp|Q09741|SPEE_SCHPO Spermidine synthase (Putrescine aminopropyltransferase) (SPDSY)
Length = 298
Score = 79.3 bits (194), Expect = 3e-15
Identities = 38/91 (41%), Positives = 57/91 (62%), Gaps = 2/91 (2%)
Frame = +2
Query: 2 ESIWFENKIVSDLVDKAKAVFPNVKYASGCVPTYPLGQIGYLLCSKNPDTNFSKPLFDVS 181
E +W ++S+++ K VFPNV+YA +PTYP G IG+++ SK+ + SKPL S
Sbjct: 203 ECMWIHLGVISNVLTAVKTVFPNVRYAYTTIPTYPSGSIGFVVASKDASIDLSKPLRKWS 262
Query: 182 TIENQ--FKYYSSDIHTASFVLPNCMKHIIN 268
E KYY+S+IH ASFVLP + +++
Sbjct: 263 PEEENKLCKYYNSEIHAASFVLPTFARDVVD 293
>sp|Q9Y8H7|SPEE_NEUCR Spermidine synthase (Putrescine aminopropyltransferase) (SPDSY)
Length = 291
Score = 75.9 bits (185), Expect = 3e-14
Identities = 37/87 (42%), Positives = 53/87 (60%), Gaps = 2/87 (2%)
Frame = +2
Query: 2 ESIWFENKIVSDLVDKAKAVFPNVKYASGCVPTYPLGQIGYLLCSKNPDTNFSKPLFDVS 181
E+ W +++ L K VFP +YA +PTYP GQIG+++CSK+P+ N PL S
Sbjct: 201 ENQWLHLPLITKLKKDCKEVFPVAEYAYTTIPTYPSGQIGFMVCSKDPNANVKVPLRSWS 260
Query: 182 TIENQ--FKYYSSDIHTASFVLPNCMK 256
E + +YY+S+IH ASF+LP K
Sbjct: 261 QEEEEKLCRYYNSEIHKASFILPTFAK 287
>sp|Q9SMB1|SPD1_ORYSA Spermidine synthase 1 (Putrescine aminopropyltransferase 1) (SPDSY
1)
Length = 323
Score = 75.1 bits (183), Expect = 5e-14
Identities = 41/98 (41%), Positives = 60/98 (61%), Gaps = 10/98 (10%)
Frame = +2
Query: 2 ESIWFENKIVSDLVDKAKAVFP-NVKYASGCVPTYPLGQIGYLLCS-KNPDTNFSKPLFD 175
ESIW I+ D+V + VF +V YA VPTYP G IG++LCS + P +F P+F+
Sbjct: 223 ESIWLHMHIIEDIVANCRQVFKGSVNYAWTTVPTYPSGVIGFMLCSTEGPTVDFQHPIFN 282
Query: 176 VSTIENQF--------KYYSSDIHTASFVLPNCMKHII 265
+ +N+F K+Y+S+IH+ASF LP+ K +I
Sbjct: 283 IE--DNEFSTKSKGPLKFYNSEIHSASFCLPSFAKRVI 318
>sp|Q12074|SPEE_YEAST Spermidine synthase (Putrescine aminopropyltransferase) (SPDSY)
Length = 293
Score = 73.9 bits (180), Expect = 1e-13
Identities = 35/83 (42%), Positives = 50/83 (60%), Gaps = 2/83 (2%)
Frame = +2
Query: 2 ESIWFENKIVSDLVDKAKAVFPNVKYASGCVPTYPLGQIGYLLCSKNPDTNFSKPLFDVS 181
ES+W I+ DL VFP +Y+ +PTYP G IG+++CSK+ N KPL ++S
Sbjct: 203 ESMWIHLPIIKDLKKACSEVFPVAEYSFVTIPTYPTGTIGFMVCSKDKTCNVKKPLREIS 262
Query: 182 TIENQ--FKYYSSDIHTASFVLP 244
+ ++YY+ IH ASFVLP
Sbjct: 263 DEKEAELYRYYNKKIHEASFVLP 285
>sp|O48661|SPD2_ARATH Spermidine synthase 2 (Putrescine aminopropyltransferase 2) (SPDSY
2)
Length = 340
Score = 73.6 bits (179), Expect = 1e-13
Identities = 38/97 (39%), Positives = 59/97 (60%), Gaps = 8/97 (8%)
Frame = +2
Query: 2 ESIWFENKIVSDLVDKAKAVFP-NVKYASGCVPTYPLGQIGYLLC-SKNPDTNFSKPLFD 175
ES+W I+ D+V + +F +V YA VPTYP G IG++LC S+ P +F KP+
Sbjct: 240 ESLWLHMDIIEDIVSNCRDIFKGSVNYAWTSVPTYPSGVIGFMLCSSEGPQVDFKKPVSL 299
Query: 176 VSTIENQ------FKYYSSDIHTASFVLPNCMKHIIN 268
+ T E+ KYY+++IH+A+F LP+ K +I+
Sbjct: 300 IDTDESSIKSHCPLKYYNAEIHSAAFCLPSFAKKVID 336
>sp|Q12455|SPSY_YEAST Spermine synthase (Spermidine aminopropyltransferase) (SPMSY)
Length = 300
Score = 72.4 bits (176), Expect = 3e-13
Identities = 36/90 (40%), Positives = 52/90 (57%), Gaps = 1/90 (1%)
Frame = +2
Query: 2 ESIWFENKIVSDLVDKAKAVFPNVKYASGCVPTYPLGQIGYLLCSKNPDTNFSKPLFDVS 181
E+ W K + DL + AK VFPN +Y VPTY GQ+G ++CS N + + P +S
Sbjct: 210 ENFWLNLKYLHDLKNTAKKVFPNTEYCYTMVPTYTSGQLGLIVCSNNANIPLNIPQRKIS 269
Query: 182 TIE-NQFKYYSSDIHTASFVLPNCMKHIIN 268
E + KYY+ IH+++FVLP +IN
Sbjct: 270 EQEQGKLKYYNPQIHSSAFVLPTWADKVIN 299
>sp|Q9ZUB3|SPD1_ARATH Spermidine synthase 1 (Putrescine aminopropyltransferase 1) (SPDSY
1)
Length = 334
Score = 71.6 bits (174), Expect = 5e-13
Identities = 37/94 (39%), Positives = 56/94 (59%), Gaps = 6/94 (6%)
Frame = +2
Query: 2 ESIWFENKIVSDLVDKAKAVFP-NVKYASGCVPTYPLGQIGYLLCS-KNPDTNFSKPLFD 175
ES+W I+ D+V + +F +V YA VPTYP G IG++LCS + PD +F PL
Sbjct: 236 ESLWLHMDIIEDIVSNCREIFKGSVNYAWTSVPTYPSGVIGFMLCSTEGPDVDFKHPLNP 295
Query: 176 V----STIENQFKYYSSDIHTASFVLPNCMKHII 265
+ S K+Y+++IH+A+F LP+ K +I
Sbjct: 296 IDESSSKSNGPLKFYNAEIHSAAFCLPSFAKKVI 329
>sp|O48659|SPD2_HYONI Spermidine synthase 2 (Putrescine aminopropyltransferase 2) (SPDSY
2)
Length = 308
Score = 70.9 bits (172), Expect = 9e-13
Identities = 37/96 (38%), Positives = 56/96 (58%), Gaps = 8/96 (8%)
Frame = +2
Query: 2 ESIWFENKIVSDLVDKAKAVFP-NVKYASGCVPTYPLGQIGYLLCS-KNPDTNFSKPLFD 175
ESIW I+ D+V + +F +V YA VPTYP G IG++LCS + P +F P+
Sbjct: 208 ESIWLHMHIIEDIVSNCRQIFKGSVNYAWTTVPTYPSGVIGFMLCSTEGPAVDFKNPINP 267
Query: 176 VSTIENQ------FKYYSSDIHTASFVLPNCMKHII 265
+ ++ K+Y+S+IH+ASF LP+ K +I
Sbjct: 268 IDADDSHTKTRGPLKFYNSEIHSASFCLPSFAKRVI 303
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39,508,413
Number of Sequences: 369166
Number of extensions: 753364
Number of successful extensions: 2131
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2049
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2089
length of database: 68,354,980
effective HSP length: 96
effective length of database: 50,620,420
effective search space used: 1721094280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)