Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_020_G05
(508 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9VQ62|NPC2_DROME Hypothetical protein NPC2 precursor (N... 59 7e-09
sp|P61917|NPC2_PANTR Epididymal secretory protein E1 precur... 57 3e-08
sp|Q9Z0J0|NPC2_MOUSE Epididymal secretory protein E1 precur... 57 3e-08
sp|P79345|NPC2_BOVIN Epididymal secretory protein E1 precur... 56 4e-08
sp|O97763|NPC2_PIG Epididymal secretory protein E1 precurso... 55 9e-08
sp|Q9DGJ3|NPC2_BRARE Epididymal secretory protein E1 precur... 49 5e-06
sp|Q28895|NPC2_CANFA Epididymal secretory protein E1 precur... 47 3e-05
sp|Q25481|ES16_MANSE Ecdysteroid regulated 16 kDa protein p... 40 0.003
sp|P38719|DBP8_YEAST Probable ATP-dependent RNA helicase DB... 30 3.2
sp|Q9FGZ4|WRK48_ARATH Probable WRKY transcription factor 48... 30 4.2
>sp|Q9VQ62|NPC2_DROME Hypothetical protein NPC2 precursor (Niemann Pick type C2 protein
homolog)
Length = 148
Score = 58.9 bits (141), Expect = 7e-09
Identities = 29/78 (37%), Positives = 40/78 (51%)
Frame = +3
Query: 198 KVSVHGKVGIFNLPFSLPDNDLXXXXXXXXXXXXQTNSTYTNSIDILKYYPSVRVTITWE 377
K VHGKV +PF L + D + YT ++ +L+ YP V V + WE
Sbjct: 70 KTVVHGKVLGIEMPFPLANPDACVDSGLKCPLEKDESYRYTATLPVLRSYPKVSVLVKWE 129
Query: 378 LIDADGNDIVCVQMPTSI 431
L D DG DI+CV++P I
Sbjct: 130 LQDQDGADIICVEIPAKI 147
Score = 32.3 bits (72), Expect = 0.65
Identities = 18/50 (36%), Positives = 24/50 (48%), Gaps = 2/50 (4%)
Frame = +1
Query: 58 YKDCGSKKGIVQSVEITPCST--QPCQLKKGTKPQFTIKYKSNENVSSEK 201
+ DCGSK G V I C T C LK+ T F+I + E ++ K
Sbjct: 21 FSDCGSKTGKFTRVAIEGCDTTKAECILKRNTTVSFSIDFALAEEATAVK 70
>sp|P61917|NPC2_PANTR Epididymal secretory protein E1 precursor (Niemann Pick type C2
protein homolog) (EPI-1)
sp|P61918|NPC2_MACFA Epididymal secretory protein E1 precursor (Niemann Pick type C2
protein homolog) (Epididymal secretory protein 14.6)
(ESP14.6)
sp|P61916|NPC2_HUMAN Epididymal secretory protein E1 precursor (Niemann-Pick disease
type C2 protein) (hE1)
Length = 151
Score = 56.6 bits (135), Expect = 3e-08
Identities = 24/61 (39%), Positives = 34/61 (55%)
Frame = +1
Query: 19 LSFINISLTEPTIYKDCGSKKGIVQSVEITPCSTQPCQLKKGTKPQFTIKYKSNENVSSE 198
L+ + EP +KDCGS G+++ V ++PC TQPCQL KG + + SN S
Sbjct: 11 LALSTAAQAEPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSS 70
Query: 199 K 201
K
Sbjct: 71 K 71
Score = 43.5 bits (101), Expect = 3e-04
Identities = 23/80 (28%), Positives = 38/80 (47%)
Frame = +3
Query: 198 KVSVHGKVGIFNLPFSLPDNDLXXXXXXXXXXXXQTNSTYTNSIDILKYYPSVRVTITWE 377
K VHG + +PF +P+ D +T S Y N + + YPS+++ + W+
Sbjct: 71 KAVVHGILMGVPVPFPIPEPDGCKSGINCPIQKDKTYS-YLNKLPVKSEYPSIKLVVEWQ 129
Query: 378 LIDADGNDIVCVQMPTSIVS 437
L D + C ++P IVS
Sbjct: 130 LQDDKNQSLFCWEIPVQIVS 149
>sp|Q9Z0J0|NPC2_MOUSE Epididymal secretory protein E1 precursor (Niemann Pick type C2
protein homolog) (mE1)
Length = 149
Score = 56.6 bits (135), Expect = 3e-08
Identities = 25/56 (44%), Positives = 34/56 (60%)
Frame = +1
Query: 10 ILGLSFINISLTEPTIYKDCGSKKGIVQSVEITPCSTQPCQLKKGTKPQFTIKYKS 177
IL L+ + S EP +KDCGSK G+++ V ++PC T PCQL KG I + S
Sbjct: 8 ILLLALVAASQAEPLHFKDCGSKVGVIKEVNVSPCPTDPCQLHKGQSYSVNITFTS 63
Score = 42.0 bits (97), Expect = 8e-04
Identities = 21/77 (27%), Positives = 37/77 (48%)
Frame = +3
Query: 207 VHGKVGIFNLPFSLPDNDLXXXXXXXXXXXXQTNSTYTNSIDILKYYPSVRVTITWELID 386
VHG + +PF +P+ D + S Y N + + YPS+++ + W+L D
Sbjct: 74 VHGILEGIRVPFPIPEPDGCKSGINCPIQKDKVYS-YLNKLPVKNEYPSIKLVVEWKLED 132
Query: 387 ADGNDIVCVQMPTSIVS 437
N++ C ++P I S
Sbjct: 133 DKKNNLFCWEIPVQITS 149
>sp|P79345|NPC2_BOVIN Epididymal secretory protein E1 precursor (Niemann Pick type C2
protein homolog) (EPV20)
Length = 149
Score = 56.2 bits (134), Expect = 4e-08
Identities = 24/66 (36%), Positives = 37/66 (56%)
Frame = +1
Query: 4 IVILGLSFINISLTEPTIYKDCGSKKGIVQSVEITPCSTQPCQLKKGTKPQFTIKYKSNE 183
+ L L+ +L EP +KDCGS G+++ V ++PC TQPC+L +G + + SN
Sbjct: 6 VAFLFLALSASALAEPVKFKDCGSWVGVIKEVNVSPCPTQPCKLHRGQSYSVNVTFTSNT 65
Query: 184 NVSSEK 201
S K
Sbjct: 66 QSQSSK 71
Score = 44.3 bits (103), Expect = 2e-04
Identities = 23/78 (29%), Positives = 36/78 (46%)
Frame = +3
Query: 198 KVSVHGKVGIFNLPFSLPDNDLXXXXXXXXXXXXQTNSTYTNSIDILKYYPSVRVTITWE 377
K VHG V +PF +P++D +T + Y N + + YPS++V + WE
Sbjct: 71 KAVVHGIVMGIPVPFPIPESDGCKSGIRCPIEKDKTYN-YVNKLPVKNEYPSIKVVVEWE 129
Query: 378 LIDADGNDIVCVQMPTSI 431
L D C Q+P +
Sbjct: 130 LTDDKNQRFFCWQIPIEV 147
>sp|O97763|NPC2_PIG Epididymal secretory protein E1 precursor (Niemann Pick type C2
protein homolog) (16 kDa secretory protein)
Length = 149
Score = 55.1 bits (131), Expect = 9e-08
Identities = 22/55 (40%), Positives = 31/55 (56%)
Frame = +1
Query: 37 SLTEPTIYKDCGSKKGIVQSVEITPCSTQPCQLKKGTKPQFTIKYKSNENVSSEK 201
+L EP ++DCGS G+++ V + PC TQPCQL KG + + SN K
Sbjct: 17 ALAEPVHFRDCGSGVGVIKEVNVNPCPTQPCQLHKGQSYSVNVTFTSNTQSKGSK 71
Score = 45.4 bits (106), Expect = 7e-05
Identities = 26/80 (32%), Positives = 38/80 (47%)
Frame = +3
Query: 198 KVSVHGKVGIFNLPFSLPDNDLXXXXXXXXXXXXQTNSTYTNSIDILKYYPSVRVTITWE 377
K VHG V +PF +PD D QT S Y N + + YPS+++ + W+
Sbjct: 71 KAVVHGIVMGVPIPFPIPDPDGCKSGINCPIQKDQTYS-YLNKLPVKAEYPSIKLVVEWK 129
Query: 378 LIDADGNDIVCVQMPTSIVS 437
L D + + C Q+P I S
Sbjct: 130 LQDDNDQCLFCWQIPVQIES 149
>sp|Q9DGJ3|NPC2_BRARE Epididymal secretory protein E1 precursor (Niemann Pick type C2
protein homolog) (16.5 kDa secretory protein)
Length = 149
Score = 49.3 bits (116), Expect = 5e-06
Identities = 23/61 (37%), Positives = 32/61 (52%)
Frame = +1
Query: 19 LSFINISLTEPTIYKDCGSKKGIVQSVEITPCSTQPCQLKKGTKPQFTIKYKSNENVSSE 198
LSF+ + +P + DCGS G V V+I PCS QPC+L KG + + S +
Sbjct: 11 LSFLAYTCADPVKFVDCGSVDGKVVQVDIKPCSQQPCKLHKGQSYTVNVTFSSGVESQTS 70
Query: 199 K 201
K
Sbjct: 71 K 71
Score = 44.7 bits (104), Expect = 1e-04
Identities = 22/80 (27%), Positives = 36/80 (45%)
Frame = +3
Query: 198 KVSVHGKVGIFNLPFSLPDNDLXXXXXXXXXXXXQTNSTYTNSIDILKYYPSVRVTITWE 377
K VHG + +PF +P +D Q Y + + YP+++V + WE
Sbjct: 71 KAVVHGVLAGVPVPFPIPIDD-GCKSGIQCPIVPQKPYNYVTELPVKTEYPAIKVVVEWE 129
Query: 378 LIDADGNDIVCVQMPTSIVS 437
L D D+ C++ P IV+
Sbjct: 130 LRDDSSKDLFCIKFPVQIVN 149
>sp|Q28895|NPC2_CANFA Epididymal secretory protein E1 precursor (Niemann Pick type C2
protein homolog) (cE1)
Length = 149
Score = 46.6 bits (109), Expect = 3e-05
Identities = 18/48 (37%), Positives = 27/48 (56%)
Frame = +1
Query: 58 YKDCGSKKGIVQSVEITPCSTQPCQLKKGTKPQFTIKYKSNENVSSEK 201
+KDCGS G+++ + + PC QPC+L KG + + SN S K
Sbjct: 24 FKDCGSAVGVIKELNVNPCPAQPCKLHKGQSYSVNVTFTSNIPSQSSK 71
Score = 39.3 bits (90), Expect = 0.005
Identities = 21/78 (26%), Positives = 36/78 (46%)
Frame = +3
Query: 198 KVSVHGKVGIFNLPFSLPDNDLXXXXXXXXXXXXQTNSTYTNSIDILKYYPSVRVTITWE 377
K VHG V +PF +P+ D +T S Y N + + YPS+++ + W
Sbjct: 71 KAVVHGIVLGVAVPFPIPEADGCKSGINCPIQKDKTYS-YLNKLPVKNEYPSIKLVVQWM 129
Query: 378 LIDADGNDIVCVQMPTSI 431
L+ + + C ++P I
Sbjct: 130 LLGDNNQHLFCWEIPVQI 147
>sp|Q25481|ES16_MANSE Ecdysteroid regulated 16 kDa protein precursor
Length = 145
Score = 40.0 bits (92), Expect = 0.003
Identities = 22/75 (29%), Positives = 32/75 (42%)
Frame = +3
Query: 207 VHGKVGIFNLPFSLPDNDLXXXXXXXXXXXXQTNSTYTNSIDILKYYPSVRVTITWELID 386
VHG + +PF D + Y ++ +LK YP V V + WEL
Sbjct: 71 VHGVIMSLPVPFPCRSPDACKDNGLTCPIKAGVVANYKTTLPVLKSYPKVSVDVKWEL-K 129
Query: 387 ADGNDIVCVQMPTSI 431
D D+VC+ +P I
Sbjct: 130 KDEEDLVCILIPARI 144
Score = 33.1 bits (74), Expect = 0.38
Identities = 17/48 (35%), Positives = 27/48 (56%), Gaps = 2/48 (4%)
Frame = +1
Query: 58 YKDCGSKKGIVQSVEIT--PCSTQPCQLKKGTKPQFTIKYKSNENVSS 195
Y DCGSK VQSV ++ P + + C LK+ + +I + +VS+
Sbjct: 19 YTDCGSKLATVQSVGVSGWPENARECVLKRNSNVTISIDFSPTTDVSA 66
>sp|P38719|DBP8_YEAST Probable ATP-dependent RNA helicase DBP8 (DEAD-box protein 8)
Length = 431
Score = 30.0 bits (66), Expect = 3.2
Identities = 16/57 (28%), Positives = 31/57 (54%), Gaps = 3/57 (5%)
Frame = +1
Query: 115 STQPCQLKKGTKPQFTIKYKSNENV---SSEKFQFTVKSEFLIYHFLYRTMTCVNFQ 276
S Q ++KG P F + +S +NV S+ K ++ + E + +LY+ +TC ++
Sbjct: 197 SLQNAPVQKGKPPLFAYQVESVDNVAIPSTLKIEYILVPEHVKEAYLYQLLTCEEYE 253
>sp|Q9FGZ4|WRK48_ARATH Probable WRKY transcription factor 48 (WRKY DNA-binding protein 48)
Length = 399
Score = 29.6 bits (65), Expect = 4.2
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = +1
Query: 130 QLKKGTKPQFTIKYKSNENVSSEKFQFTVKSE 225
Q +KGTKPQ K K+ + +F F KS+
Sbjct: 185 QEQKGTKPQLKAKKKNQKKAREARFAFLTKSD 216
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 51,793,334
Number of Sequences: 369166
Number of extensions: 929544
Number of successful extensions: 1964
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1906
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1961
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3206272875
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)