Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_020_E11
(687 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9BV86|CI032_HUMAN Protein C9orf32 180 4e-45
sp|Q5BJX0|CI032_RAT Protein C9orf32 homolog 179 6e-45
sp|Q8R2U4|CI032_MOUSE Protein C9orf32 homolog 178 1e-44
sp|P38340|YB9H_YEAST Protein YBR261C 134 2e-31
sp|O13748|YDRE_SCHPO Hypothetical protein C16E8.14c in chro... 128 2e-29
sp|Q98MF0|PYRG_RHILO CTP synthase (UTP--ammonia ligase) (CT... 33 0.54
sp|Q8TU79|SYA_METAC Alanyl-tRNA synthetase (Alanine--tRNA l... 31 2.7
sp|Q98KB1|MURD_RHILO UDP-N-acetylmuramoylalanine--D-glutama... 31 2.7
sp|Q92QA0|PYRG_RHIME CTP synthase (UTP--ammonia ligase) (CT... 30 4.6
sp|Q58055|Y638_METJA Hypothetical protein MJ0638 30 4.6
>sp|Q9BV86|CI032_HUMAN Protein C9orf32
Length = 223
Score = 180 bits (456), Expect = 4e-45
Identities = 92/209 (44%), Positives = 127/209 (60%), Gaps = 1/209 (0%)
Frame = +1
Query: 4 RKYWKEVSPTVNGMLGGFEIVSDGDIKTSTXXXXXXXXXGFLTKSTGKALDCGSGIGRVT 183
+ YWK++ PTV+GMLGG+ +S DI +S G T ALDCG+GIGR+T
Sbjct: 17 KTYWKQIPPTVDGMLGGYGHISSIDINSSRKFLQRFLREGPNKTGTSCALDCGAGIGRIT 76
Query: 184 KNLLMPIFPKVDMVEVTKHFLDESYKYIGETSQSI-NRICCSLQNFTPNQNDYDLIWCQW 360
K LL+P+F +VDMV++T+ FL ++ Y+GE + + N CC LQ+FTP + YD+IW QW
Sbjct: 77 KRLLLPLFREVDMVDITEDFLVQAKTYLGEEGKRVRNYFCCGLQDFTPEPDSYDVIWIQW 136
Query: 361 VTGHLTDQALVEFLERCITGLHSKNSLIVIKENVVKGNXXXXXXXXXXXTRPISLLKRLF 540
V GHLTDQ L EFL RC G N +IVIK+N+ + R + +++R+
Sbjct: 137 VIGHLTDQHLAEFLRRC-KGSLRPNGIIVIKDNMAQ-EGVILDDVDSSVCRDLDVVRRII 194
Query: 541 VQANLELIKEERQTEFPKELYPVWMFVLR 627
A L L+ EERQ P E+Y V+ F LR
Sbjct: 195 CSAGLSLLAEERQENLPDEIYHVYSFALR 223
>sp|Q5BJX0|CI032_RAT Protein C9orf32 homolog
Length = 223
Score = 179 bits (454), Expect = 6e-45
Identities = 92/209 (44%), Positives = 128/209 (61%), Gaps = 1/209 (0%)
Frame = +1
Query: 4 RKYWKEVSPTVNGMLGGFEIVSDGDIKTSTXXXXXXXXXGFLTKSTGKALDCGSGIGRVT 183
+ YWK++ PTV+GMLGG+ +S+ D+ +S G T ALDCG+GIGR+T
Sbjct: 17 KTYWKQIPPTVDGMLGGYGHISNIDLNSSRKFLQRFLREGPNKTGTSCALDCGAGIGRIT 76
Query: 184 KNLLMPIFPKVDMVEVTKHFLDESYKYIGETSQSI-NRICCSLQNFTPNQNDYDLIWCQW 360
K LL+P+F VDMV+VT+ FL ++ Y+GE + + N CC LQ+F+P + YD+IW QW
Sbjct: 77 KRLLLPLFRVVDMVDVTEDFLAKAKTYLGEEGKRVRNYFCCGLQDFSPEPSSYDVIWIQW 136
Query: 361 VTGHLTDQALVEFLERCITGLHSKNSLIVIKENVVKGNXXXXXXXXXXXTRPISLLKRLF 540
V GHLTDQ L EFL RC GL N +IVIK+N+ + R + +++R+
Sbjct: 137 VIGHLTDQHLAEFLRRCRRGLR-PNGIIVIKDNMAQ-EGVILDDVDSSVCRDLEVVRRII 194
Query: 541 VQANLELIKEERQTEFPKELYPVWMFVLR 627
A L L+ EERQ P E+Y V+ F LR
Sbjct: 195 RSAGLSLLAEERQENLPDEIYHVYSFALR 223
>sp|Q8R2U4|CI032_MOUSE Protein C9orf32 homolog
Length = 223
Score = 178 bits (452), Expect = 1e-44
Identities = 92/209 (44%), Positives = 127/209 (60%), Gaps = 1/209 (0%)
Frame = +1
Query: 4 RKYWKEVSPTVNGMLGGFEIVSDGDIKTSTXXXXXXXXXGFLTKSTGKALDCGSGIGRVT 183
+ YWK++ PTV+GMLGG+ +S+ D+ +S G T ALDCG+GIGR+T
Sbjct: 17 KTYWKQIPPTVDGMLGGYGHISNIDLNSSRKFLQRFLREGPNKTGTSCALDCGAGIGRIT 76
Query: 184 KNLLMPIFPKVDMVEVTKHFLDESYKYIGETSQSI-NRICCSLQNFTPNQNDYDLIWCQW 360
K LL+P+F VDMV+VT+ FL ++ Y+GE + + N CC LQ+F+P YD+IW QW
Sbjct: 77 KRLLLPLFRVVDMVDVTEDFLAKAKTYLGEEGKRVRNYFCCGLQDFSPEPGSYDVIWIQW 136
Query: 361 VTGHLTDQALVEFLERCITGLHSKNSLIVIKENVVKGNXXXXXXXXXXXTRPISLLKRLF 540
V GHLTDQ L EFL RC GL N +IVIK+N+ + R + +++R+
Sbjct: 137 VIGHLTDQHLAEFLRRCKRGLR-PNGIIVIKDNMAQ-EGVILDDVDSSVCRDLEVVRRII 194
Query: 541 VQANLELIKEERQTEFPKELYPVWMFVLR 627
A L L+ EERQ P E+Y V+ F LR
Sbjct: 195 RTAGLSLLAEERQENLPDEIYHVYSFALR 223
>sp|P38340|YB9H_YEAST Protein YBR261C
Length = 232
Score = 134 bits (337), Expect = 2e-31
Identities = 81/217 (37%), Positives = 118/217 (54%), Gaps = 7/217 (3%)
Frame = +1
Query: 10 YWKEVSPTVNGMLGGF---EIVSDGDIKTSTXXXXXXXXXGFLTKSTGK-ALDCGSGIGR 177
YW +V TV+G+LGG+ +V D+ S ++ K A+D G+GIGR
Sbjct: 17 YWTDVDATVDGVLGGYGEGTVVPTMDVLGSNNFLRKLKSRMLPQENNVKYAVDIGAGIGR 76
Query: 178 VTKNLLMPIFPKVDMVEVTKHFLDESYKYIGETSQS--INRIC-CSLQNFTPNQNDYDLI 348
V+K +L K+D+VE K F+++ + + E I +I +Q++TP+ Y LI
Sbjct: 77 VSKTMLHKHAAKIDLVEPVKPFIEQMHVELAELKDKGQIGQIYEVGMQDWTPDAGKYWLI 136
Query: 349 WCQWVTGHLTDQALVEFLERCITGLHSKNSLIVIKENVVKGNXXXXXXXXXXXTRPISLL 528
WCQW GHL D LV FL+RCI GL N IV+KEN + TR +
Sbjct: 137 WCQWCVGHLPDAELVAFLKRCIVGL-QPNGTIVVKENNTPTDTDDFDETDSSVTRSDAKF 195
Query: 529 KRLFVQANLELIKEERQTEFPKELYPVWMFVLRPISN 639
+++F +A L+LI ERQ P+ELYPV M+ L+P+ N
Sbjct: 196 RQIFEEAGLKLIASERQRGLpreLYPVRMYALKPMPN 232
>sp|O13748|YDRE_SCHPO Hypothetical protein C16E8.14c in chromosome I
Length = 219
Score = 128 bits (321), Expect = 2e-29
Identities = 81/213 (38%), Positives = 111/213 (52%), Gaps = 3/213 (1%)
Frame = +1
Query: 10 YWKEVSPTVNGMLGGF---EIVSDGDIKTSTXXXXXXXXXGFLTKSTGKALDCGSGIGRV 180
YW V PTV+GMLGG I + + T G + A DCG+GIGRV
Sbjct: 13 YWNGVQPTVDGMLGGLGTGRIPQTDVVGSRTFLNRLNYRIGKIENLV--AADCGAGIGRV 70
Query: 181 TKNLLMPIFPKVDMVEVTKHFLDESYKYIGETSQSINRICCSLQNFTPNQNDYDLIWCQW 360
T+N+L+ I VD+VE ++F+ + K + S + LQN+TP +N Y LIW QW
Sbjct: 71 TENVLLKIASHVDLVEPVENFISTAKKQLATKPCSFINV--GLQNWTPEKNRYGLIWNQW 128
Query: 361 VTGHLTDQALVEFLERCITGLHSKNSLIVIKENVVKGNXXXXXXXXXXXTRPISLLKRLF 540
HLTD+ L+ +L RC + K +I +KEN V TR LK LF
Sbjct: 129 CLSHLTDEDLIAYLSRCCEAIQEK-GVICVKEN-VSSFEDTFDPIDSSVTRCEQSLKSLF 186
Query: 541 VQANLELIKEERQTEFPKELYPVWMFVLRPISN 639
+ANL ++ E Q FP+EL+PV M+ L P S+
Sbjct: 187 KKANLVVVAETLQHGFPEELFPVKMYALVPHSS 219
>sp|Q98MF0|PYRG_RHILO CTP synthase (UTP--ammonia ligase) (CTP synthetase)
Length = 542
Score = 33.5 bits (75), Expect = 0.54
Identities = 31/133 (23%), Positives = 54/133 (40%), Gaps = 3/133 (2%)
Frame = +1
Query: 16 KEVSPTVNGMLGGFEIVSDGDIKTSTXXXXXXXXXGFLTKSTGKALDCGSGI--GRVTKN 189
K++ P +N G G++ + G + TG++ + I GR+ KN
Sbjct: 38 KKLDPYLNVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGRSANQQDNITTGRIYKN 97
Query: 190 LLMPIFPKVDMVEVTKHFLDESYKYIGETSQSINRICCSLQNFTPNQN-DYDLIWCQWVT 366
++ + Y+G T Q I + ++NF N N DYD + C+ +
Sbjct: 98 IIER---------------ERRGDYLGATVQVIPHVTDEIKNFVLNGNDDYDFVLCE-IG 141
Query: 367 GHLTDQALVEFLE 405
G + D + FLE
Sbjct: 142 GTVGDIEAMPFLE 154
>sp|Q8TU79|SYA_METAC Alanyl-tRNA synthetase (Alanine--tRNA ligase) (AlaRS)
Length = 925
Score = 31.2 bits (69), Expect = 2.7
Identities = 24/76 (31%), Positives = 38/76 (50%), Gaps = 1/76 (1%)
Frame = -2
Query: 254 DSSKKCLVTSTMSTLGKIGISKFLVTRPIPDPQSRALPVDFVRNPLLIKFNNKFVDVLIS 75
D + C T +ST G IG K L T I D R V+F ++ K +L+
Sbjct: 709 DDVEACGGTHCLST-GVIGPIKILKTERIQDGVER---VEFAAGIAAVRAMQKMESLLVD 764
Query: 74 PSETISKPP-NIPLTV 30
++T+S PP ++P++V
Sbjct: 765 SAKTLSVPPEHLPVSV 780
>sp|Q98KB1|MURD_RHILO UDP-N-acetylmuramoylalanine--D-glutamate ligase
(UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase)
(D-glutamic acid-adding enzyme)
Length = 466
Score = 31.2 bits (69), Expect = 2.7
Identities = 30/111 (27%), Positives = 44/111 (39%)
Frame = +1
Query: 127 LTKSTGKALDCGSGIGRVTKNLLMPIFPKVDMVEVTKHFLDESYKYIGETSQSINRICCS 306
+ KS G+ G IGR L D + +HF+ E Y + + SIN
Sbjct: 135 ILKSAGRDTQMGGNIGRAVMTL--------DPPKPDRHFVVECSSYQIDLAPSINPTAGI 186
Query: 307 LQNFTPNQNDYDLIWCQWVTGHLTDQALVEFLERCITGLHSKNSLIVIKEN 459
L N TP+ D H T Q ER + G S+ ++I I ++
Sbjct: 187 LLNLTPDHLD----------RHGTMQHYASIKERLVAG--SETAIIGIDDS 225
>sp|Q92QA0|PYRG_RHIME CTP synthase (UTP--ammonia ligase) (CTP synthetase)
Length = 542
Score = 30.4 bits (67), Expect = 4.6
Identities = 18/62 (29%), Positives = 33/62 (53%), Gaps = 3/62 (4%)
Frame = +1
Query: 229 VTKHFLDESYK--YIGETSQSINRICCSLQNF-TPNQNDYDLIWCQWVTGHLTDQALVEF 399
+ K+ +D+ + Y+G T Q I + ++NF T DYD + C+ + G + D + F
Sbjct: 94 IYKNIIDKERRGDYLGATVQVIPHVTNEIKNFVTEGNEDYDFVICE-IGGTVGDIEAMPF 152
Query: 400 LE 405
+E
Sbjct: 153 ME 154
>sp|Q58055|Y638_METJA Hypothetical protein MJ0638
Length = 225
Score = 30.4 bits (67), Expect = 4.6
Identities = 30/122 (24%), Positives = 47/122 (38%), Gaps = 7/122 (5%)
Frame = +1
Query: 124 FLTKSTGKALDCGSGIGRVTKNLLMPIFPKVDMVEVTKHF----LDESYKYIGETSQSIN 291
F K LDCG G G +TK F LD S +
Sbjct: 42 FENKKDFLVLDCGCGFGA--------------FYNLTKDFNTIYLDISLNLLKRFKLKER 87
Query: 292 RICCSLQNFTPNQNDYDLIWCQWVTGHLTDQALVEFLERCITGLHSKNSLIVI---KENV 462
+IC ++ + N +DL+ C V H+ + + R L +K LIV+ K+++
Sbjct: 88 KICANILHLPFKDNTFDLVLCINVLEHVNYLKALNEIRRI---LKNKGKLIVVVVNKDSL 144
Query: 463 VK 468
+K
Sbjct: 145 IK 146
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 70,916,528
Number of Sequences: 369166
Number of extensions: 1283926
Number of successful extensions: 3223
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3136
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3210
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 5879188535
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)