Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_020_D10
(378 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P35603|AP50_CAEEL Clathrin coat assembly protein AP50 (C... 119 2e-27
sp|P84092|AP2M1_RAT Clathrin coat assembly protein AP50 (Cl... 116 1e-26
sp|P54672|AP50_DICDI Clathrin coat assembly protein AP50 (C... 62 4e-10
sp|Q99186|APM4_YEAST Adaptin medium chain homolog APM4 56 3e-08
sp|P35602|AP47_CAEEL Clathrin coat assembly protein AP47 (C... 53 2e-07
sp|Q9BXS5|AP1M1_HUMAN Adaptor-related protein complex 1, mu... 52 6e-07
sp|P35585|AP1M1_MOUSE Adaptor-related protein complex 1, mu... 52 6e-07
sp|Q9Y6Q5|AP1M2_HUMAN Adaptor-related protein complex 1, mu... 51 7e-07
sp|Q9WVP1|AP1M2_MOUSE Adaptor-related protein complex 1, mu... 51 7e-07
sp|Q09718|AP50_SCHPO Probable clathrin coat assembly protei... 47 1e-05
>sp|P35603|AP50_CAEEL Clathrin coat assembly protein AP50 (Clathrin coat-associated
protein AP50) (Plasma membrane adaptor AP-2 50 kDa
protein) (Clathrin assembly protein complex 2 medium
chain) (Dumpy protein 23)
Length = 441
Score = 119 bits (298), Expect = 2e-27
Identities = 54/67 (80%), Positives = 61/67 (91%), Gaps = 1/67 (1%)
Frame = +2
Query: 2 DLLTVGD-RQKRWTRPPISMNFEVPFAPSGFKVRYLKVFESKLNYSDHEVIKWVRYIGKS 178
DLL+ G+ +K+W RPP+SMNFEVPFAPSG KVRYLKVFE KLNYSDH+VIKWVRYIG+S
Sbjct: 375 DLLSTGNVEKKKWNRPPVSMNFEVPFAPSGLKVRYLKVFEPKLNYSDHDVIKWVRYIGRS 434
Query: 179 GLYETRC 199
GLYETRC
Sbjct: 435 GLYETRC 441
>sp|P84092|AP2M1_RAT Clathrin coat assembly protein AP50 (Clathrin coat-associated
protein AP50) (Plasma membrane adaptor AP-2 50 kDa
protein) (Clathrin assembly protein complex 2 medium
chain) (AP-2 mu 2 chain)
sp|P84091|AP2M1_MOUSE Clathrin coat assembly protein AP50 (Clathrin coat-associated
protein AP50) (Plasma membrane adaptor AP-2 50 kDa
protein) (Clathrin assembly protein complex 2 medium
chain) (AP-2 mu 2 chain)
sp|Q96CW1|AP2M1_HUMAN Clathrin coat assembly protein AP50 (Clathrin coat-associated
protein AP50) (Plasma membrane adaptor AP-2 50 kDa
protein) (HA2 50 kDa subunit) (Clathrin assembly protein
complex 2 medium chain) (AP-2 mu 2 chain)
Length = 435
Score = 116 bits (291), Expect = 1e-26
Identities = 53/66 (80%), Positives = 59/66 (89%)
Frame = +2
Query: 2 DLLTVGDRQKRWTRPPISMNFEVPFAPSGFKVRYLKVFESKLNYSDHEVIKWVRYIGKSG 181
+LL D+ K+W RPPISMNFEVPFAPSG KVRYLKVFE KLNYSDH+VIKWVRYIG+SG
Sbjct: 371 ELLPTNDK-KKWARPPISMNFEVPFAPSGLKVRYLKVFEPKLNYSDHDVIKWVRYIGRSG 429
Query: 182 LYETRC 199
+YETRC
Sbjct: 430 IYETRC 435
>sp|P54672|AP50_DICDI Clathrin coat assembly protein AP50 (Clathrin coat-associated
protein AP50) (Plasma membrane adaptor AP-2 50 kDa
protein) (Clathrin assembly protein complex 2 medium
chain)
Length = 439
Score = 62.0 bits (149), Expect = 4e-10
Identities = 33/58 (56%), Positives = 41/58 (70%), Gaps = 1/58 (1%)
Frame = +2
Query: 26 QKRWTRPPISMNFEVP-FAPSGFKVRYLKVFESKLNYSDHEVIKWVRYIGKSGLYETR 196
+K W+RPPISM F+V F SGF VR+LKV E K NY+ IKWVRY+ K+G Y+ R
Sbjct: 385 KKAWSRPPISMEFQVTMFTASGFSVRFLKVVE-KSNYTP---IKWVRYLTKAGTYQNR 438
>sp|Q99186|APM4_YEAST Adaptin medium chain homolog APM4
Length = 491
Score = 55.8 bits (133), Expect = 3e-08
Identities = 27/57 (47%), Positives = 36/57 (63%), Gaps = 1/57 (1%)
Frame = +2
Query: 29 KRWTRPPISMNFEVP-FAPSGFKVRYLKVFESKLNYSDHEVIKWVRYIGKSGLYETR 196
++WTRPPIS+ FEV F+ SG VRY + S H +KW++YI K+G YE R
Sbjct: 437 QQWTRPPISLEFEVMMFSNSGLVVRYFTISGKD---SKHRAVKWIKYISKAGSYEVR 490
>sp|P35602|AP47_CAEEL Clathrin coat assembly protein AP47 (Clathrin coat-associated
protein AP47) (Golgi adaptor AP-1 47 kDa protein) (HA1
47 kDa subunit) (Clathrin assembly protein assembly
protein complex 1 medium chain) (Uncoordinated protein
101)
Length = 422
Score = 52.8 bits (125), Expect = 2e-07
Identities = 26/53 (49%), Positives = 35/53 (66%), Gaps = 1/53 (1%)
Frame = +2
Query: 41 RPPISMNFEVP-FAPSGFKVRYLKVFESKLNYSDHEVIKWVRYIGKSGLYETR 196
RPPI + FE+P F SG +VRYLK+ E S ++ + WVRYI ++G YE R
Sbjct: 372 RPPIKVKFEIPYFTTSGIQVRYLKIIEK----SGYQALPWVRYITQNGEYEMR 420
>sp|Q9BXS5|AP1M1_HUMAN Adaptor-related protein complex 1, mu 1 subunit (Mu-adaptin 1)
(Adaptor protein complex AP-1 mu-1 subunit) (Golgi
adaptor HA1/AP1 adaptin mu-1 subunit) (Clathrin assembly
protein assembly protein complex 1 medium chain 1)
(Clathrin coat assembly protein AP47) (Clathrin
coat-associated protein AP47) (AP-mu chain family member
mu1A)
Length = 423
Score = 51.6 bits (122), Expect = 6e-07
Identities = 25/53 (47%), Positives = 36/53 (67%), Gaps = 1/53 (1%)
Frame = +2
Query: 41 RPPISMNFEVP-FAPSGFKVRYLKVFESKLNYSDHEVIKWVRYIGKSGLYETR 196
+PPIS+ FE+P F SG +VRYLK+ E S ++ + WVRYI ++G Y+ R
Sbjct: 373 KPPISVKFEIPYFTTSGIQVRYLKIIEK----SGYQALPWVRYITQNGDYQLR 421
>sp|P35585|AP1M1_MOUSE Adaptor-related protein complex 1, mu 1 subunit (Mu-adaptin 1)
(Adaptor protein complex AP-1 mu-1 subunit) (Golgi
adaptor HA1/AP1 adaptin mu-1 subunit) (Clathrin assembly
protein assembly protein complex 1 medium chain 1)
(Clathrin coat assembly protein AP47) (Clathrin
coat-associated protein AP47) (AP-mu chain family member
mu1A)
Length = 423
Score = 51.6 bits (122), Expect = 6e-07
Identities = 25/53 (47%), Positives = 36/53 (67%), Gaps = 1/53 (1%)
Frame = +2
Query: 41 RPPISMNFEVP-FAPSGFKVRYLKVFESKLNYSDHEVIKWVRYIGKSGLYETR 196
+PPIS+ FE+P F SG +VRYLK+ E S ++ + WVRYI ++G Y+ R
Sbjct: 373 KPPISVKFEIPYFTTSGIQVRYLKIIEK----SGYQALPWVRYITQNGDYQLR 421
>sp|Q9Y6Q5|AP1M2_HUMAN Adaptor-related protein complex 1, mu 2 subunit (Mu-adaptin 2)
(Adaptor protein complex AP-1 mu-2 subunit) (Golgi
adaptor HA1/AP1 adaptin mu-2 subunit) (Clathrin assembly
protein assembly protein complex 1 medium chain 2)
(AP-mu chain family member mu1B)
Length = 423
Score = 51.2 bits (121), Expect = 7e-07
Identities = 25/53 (47%), Positives = 35/53 (66%), Gaps = 1/53 (1%)
Frame = +2
Query: 41 RPPISMNFEVP-FAPSGFKVRYLKVFESKLNYSDHEVIKWVRYIGKSGLYETR 196
RPPI + FE+P F SG +VRY+K+ E S ++ + WVRYI +SG Y+ R
Sbjct: 373 RPPIGVKFEIPYFTVSGIQVRYMKIIEK----SGYQALPWVRYITQSGDYQLR 421
>sp|Q9WVP1|AP1M2_MOUSE Adaptor-related protein complex 1, mu 2 subunit (Mu-adaptin 2)
(Adaptor protein complex AP-1 mu-2 subunit) (Golgi
adaptor HA1/AP1 adaptin mu-2 subunit) (Clathrin assembly
protein assembly protein complex 1 medium chain 2)
(AP-mu chain family member mu1B)
Length = 423
Score = 51.2 bits (121), Expect = 7e-07
Identities = 25/53 (47%), Positives = 35/53 (66%), Gaps = 1/53 (1%)
Frame = +2
Query: 41 RPPISMNFEVP-FAPSGFKVRYLKVFESKLNYSDHEVIKWVRYIGKSGLYETR 196
RPPI + FE+P F SG +VRY+K+ E S ++ + WVRYI +SG Y+ R
Sbjct: 373 RPPIGVKFEIPYFTVSGIQVRYMKIIEK----SGYQALPWVRYITQSGDYQLR 421
>sp|Q09718|AP50_SCHPO Probable clathrin coat assembly protein AP50 (Clathrin
coat-associated protein AP50) (Plasma membrane adaptor
AP-2 50 kDa protein) (Clathrin assembly protein complex
2 medium chain)
Length = 446
Score = 47.4 bits (111), Expect = 1e-05
Identities = 25/58 (43%), Positives = 37/58 (63%), Gaps = 1/58 (1%)
Frame = +2
Query: 26 QKRWTRPPISMNFEV-PFAPSGFKVRYLKVFESKLNYSDHEVIKWVRYIGKSGLYETR 196
Q+ W +PPIS++F + F SG V+YL+V E + S ++ IKWVRY ++G E R
Sbjct: 390 QQIWAKPPISLDFNILMFTSSGLHVQYLRVSEP--SNSKYKSIKWVRYSTRAGTCEIR 445
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 44,925,957
Number of Sequences: 369166
Number of extensions: 802278
Number of successful extensions: 2039
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1954
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2027
length of database: 68,354,980
effective HSP length: 92
effective length of database: 51,359,360
effective search space used: 1694858880
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)