Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_020_D09
(642 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O43497|CAC1G_HUMAN Voltage-dependent T-type calcium chan... 39 0.009
sp|Q12799|TC10_HUMAN T-complex protein 10A homolog 39 0.015
sp|Q9NQU5|PAK6_HUMAN Serine/threonine-protein kinase PAK 6 ... 37 0.033
sp|P17143|J1L_HCMVA Hypothetical protein J1L 37 0.056
sp|Q08865|H12_VOLCA Histone H1-II 37 0.056
sp|Q10407|MKH1_SCHPO MAP kinase kinase kinase mkh1 36 0.074
sp|Q5PSV9|MDC1_MOUSE Mediator of DNA damage checkpoint prot... 36 0.074
sp|Q80VW5|WHRN_MOUSE Whirlin 35 0.16
sp|P54258|ATN1_RAT Atrophin-1 (Dentatorubral-pallidoluysian... 35 0.16
sp|Q8CJQ8|IF2_STRCO Translation initiation factor IF-2 35 0.16
>sp|O43497|CAC1G_HUMAN Voltage-dependent T-type calcium channel alpha-1G subunit
(Voltage-gated calcium channel alpha subunit Cav3.1)
(Cav3.1c) (NBR13)
Length = 2377
Score = 39.3 bits (90), Expect = 0.009
Identities = 36/142 (25%), Positives = 57/142 (40%), Gaps = 4/142 (2%)
Frame = +1
Query: 124 GDKTKMASRTAVIGGAAGGGNTARKPMNTVPVAPKLATSQRANMKKEKFEAMQLATAATN 303
GD K S + G +K + V + + ++K + + TAAT
Sbjct: 994 GDANKSESEPDFFSPSLDGDGDRKKCLALVSLG------EHPELRKSLLPPLIIHTAATP 1047
Query: 304 VMSLDRQA----GQPRPPASTRTALPRSATVGRVPNRKPPPPGRANPDARKETAQDKPTG 471
MSL + G+ PAS RT+ SA G K PP R++P + A +
Sbjct: 1048 -MSLPKSTSTGLGEALGPASRRTSSSGSAEPGAAHEMKSPPSARSSPHSPWSAASSWTSR 1106
Query: 472 RVPQSTSARAAGLPRSANTNSR 537
R +++ RA L R + + R
Sbjct: 1107 RSSRNSLGRAPSLKRRSPSGER 1128
>sp|Q12799|TC10_HUMAN T-complex protein 10A homolog
Length = 416
Score = 38.5 bits (88), Expect = 0.015
Identities = 32/109 (29%), Positives = 48/109 (44%), Gaps = 7/109 (6%)
Frame = +1
Query: 223 PKLA--TSQRANMKKEKFEAMQLATAATNVMSLDRQAGQPRPPASTRTALPRSATVGRVP 396
PK A SQ A + +++ + LA + +R PP R P GR
Sbjct: 140 PKYAGRKSQSATLLGQRWSSNHLAPPKPMSLKTERINSGKTPPQEDREKSPP----GRRQ 195
Query: 397 NRKPPPPGRANPDA-RKETAQD----KPTGRVPQSTSARAAGLPRSANT 528
+R P P GR P A R+E ++D P+ R PQ++ R + + S T
Sbjct: 196 DRSPAPTGRPTPGAERREVSEDGKIMHPSSRSPQNSGGRKSPVQASQAT 244
>sp|Q9NQU5|PAK6_HUMAN Serine/threonine-protein kinase PAK 6 (p21-activated kinase 6)
(PAK-6) (PAK-5)
Length = 681
Score = 37.4 bits (85), Expect = 0.033
Identities = 35/141 (24%), Positives = 59/141 (41%), Gaps = 11/141 (7%)
Frame = +1
Query: 181 GNTARKPMNTVPVAPKLATSQRANMKKEKFEAMQLATAATNVMSLDRQAGQPRPPASTRT 360
G+ +P +PK ++ +++K+ F +M L+TAAT PP+S++
Sbjct: 235 GSATGRPGGEGSPSPK---TRESSLKRRLFRSMFLSTAAT------------APPSSSKP 279
Query: 361 ALPRSATVGRVPNRKPPPPGRANPDARKETAQ----DKPTGRVPQSTSARAAGLPRSANT 528
P + PN PP + NP + AQ D+P G T++ + +S T
Sbjct: 280 GPPPQSK----PNSSFRPPQKDNPPSLVAKAQSLPSDQPVGTFSPLTTSDTSSPQKSLRT 335
Query: 529 -------NSRSAPKHQPVAWN 570
RS+P P W+
Sbjct: 336 APATGQLPGRSSPAGSPRTWH 356
>sp|P17143|J1L_HCMVA Hypothetical protein J1L
Length = 309
Score = 36.6 bits (83), Expect = 0.056
Identities = 31/92 (33%), Positives = 39/92 (42%), Gaps = 5/92 (5%)
Frame = +1
Query: 304 VMSLDRQAGQPRP----PASTRTALPRSATVGRVPNRKPPPPGRANPDARKETAQDK-PT 468
V SL R A PRP P + RTA + P PP A P R+ + K P
Sbjct: 4 VGSLVRAANCPRPQRNLPYAARTAPAPAQPPSPAPTPSRTPPVSATPRHRRRPERSKTPD 63
Query: 469 GRVPQSTSARAAGLPRSANTNSRSAPKHQPVA 564
R ++T AR + SAPKH+P A
Sbjct: 64 KRSAETTQARTV-------ERTGSAPKHRPEA 88
>sp|Q08865|H12_VOLCA Histone H1-II
Length = 241
Score = 36.6 bits (83), Expect = 0.056
Identities = 28/114 (24%), Positives = 47/114 (41%), Gaps = 1/114 (0%)
Frame = +1
Query: 226 KLATSQRANMKKE-KFEAMQLATAATNVMSLDRQAGQPRPPASTRTALPRSATVGRVPNR 402
KL+ +Q++ K K +A AA + ++A P+ + P+S P
Sbjct: 92 KLSDAQKSKAKAAAKPKAAPKKAAAPKKAAAPKKAKAPKKEGEKKAVKPKSEKKAAKPKT 151
Query: 403 KPPPPGRANPDARKETAQDKPTGRVPQSTSARAAGLPRSANTNSRSAPKHQPVA 564
+ P P A K+ A KP + P +A+ A ++A +APK A
Sbjct: 152 EKKPKAAKKPKAAKKPAAKKPAAKKP---AAKKATPKKAAAPKKAAAPKKAKAA 202
>sp|Q10407|MKH1_SCHPO MAP kinase kinase kinase mkh1
Length = 1116
Score = 36.2 bits (82), Expect = 0.074
Identities = 27/93 (29%), Positives = 43/93 (46%), Gaps = 2/93 (2%)
Frame = +1
Query: 217 VAPKLATSQRANMKKEKFEAMQLATAATNVMSLDRQAGQPRPPASTRTA--LPRSATVGR 390
+A K+ + A +K KF + T M L+ + P+ P+S +A L R+ R
Sbjct: 482 MATKILDATEAQSEKNKFTVCRPHKKVTLKMPLNSGSSAPQSPSSNTSASVLTRNFVAHR 541
Query: 391 VPNRKPPPPGRANPDARKETAQDKPTGRVPQST 489
P PPPP + RK T +P+ R +S+
Sbjct: 542 DP---PPPPTETSSLRRKNTLTRRPSIRHARSS 571
>sp|Q5PSV9|MDC1_MOUSE Mediator of DNA damage checkpoint protein 1
Length = 1707
Score = 36.2 bits (82), Expect = 0.074
Identities = 31/121 (25%), Positives = 53/121 (43%), Gaps = 5/121 (4%)
Frame = +1
Query: 214 PVAPKLATSQRANMKKEKFEAMQLATAATNVMSLDRQAGQPRPPASTRTALPRSATVGRV 393
PV PKL TSQ + + E + + S ++ R P +TR +S
Sbjct: 1076 PVIPKL-TSQVTEGRVQMPEPLLTGPEIQSPTSTEQSVTPDRKPRATRGRPSKS------ 1128
Query: 394 PNRKPPPPGRANPDARKETAQDKPTGRVPQSTSARAAGLPRSANTNS-----RSAPKHQP 558
PN+ P P P+ + T+ ++P +P+ TS G PR ++ + + P+ QP
Sbjct: 1129 PNKTPEPLISTGPELQPPTSIEQPV--IPKPTSRVTRGRPRKSSVRTPESVVSTGPELQP 1186
Query: 559 V 561
+
Sbjct: 1187 L 1187
>sp|Q80VW5|WHRN_MOUSE Whirlin
Length = 906
Score = 35.0 bits (79), Expect = 0.16
Identities = 22/63 (34%), Positives = 29/63 (46%), Gaps = 4/63 (6%)
Frame = +1
Query: 316 DRQAGQPRPPA----STRTALPRSATVGRVPNRKPPPPGRANPDARKETAQDKPTGRVPQ 483
DR GQPR P + ++ S V P + PPPG A P +AQD P+ +
Sbjct: 594 DRLLGQPRKPGREDPAPLSSAAHSGIVFSAPRNRSPPPGTA-PTPGPSSAQDSPSSPIYA 652
Query: 484 STS 492
S S
Sbjct: 653 SIS 655
>sp|P54258|ATN1_RAT Atrophin-1 (Dentatorubral-pallidoluysian atrophy protein)
Length = 1183
Score = 35.0 bits (79), Expect = 0.16
Identities = 28/97 (28%), Positives = 39/97 (40%)
Frame = +1
Query: 337 RPPASTRTALPRSATVGRVPNRKPPPPGRANPDARKETAQDKPTGRVPQSTSARAAGLPR 516
+PP T+ +LP A + P PPP GR P+ PTG QST+ A
Sbjct: 421 QPPKYTQPSLPSQAVWSQGPPPPPPPYGRLLPNNNTHPGPFPPTG--GQSTAHPPAPAHH 478
Query: 517 SANTNSRSAPKHQPVAWNY*DSMLXXXXXGLLPPNKY 627
+ P+ QP ++ +S G PP Y
Sbjct: 479 HHQQQQQPQPQPQPQQHHHGNS-------GPPPPGAY 508
>sp|Q8CJQ8|IF2_STRCO Translation initiation factor IF-2
Length = 1033
Score = 35.0 bits (79), Expect = 0.16
Identities = 37/129 (28%), Positives = 50/129 (38%), Gaps = 4/129 (3%)
Frame = +1
Query: 175 GGGNTARKPMNTVPVAPKLATSQRANMKKEKFEAMQLATAATNVMSLDRQAGQPRPPAST 354
GGGN P APK A + + + Q A AA + + R + P+ PA+
Sbjct: 53 GGGNGRSAGR---PAAPKKAAPRPSAPSPAQAGPSQAAPAAGDRAAAPRPSAAPKAPAAQ 109
Query: 355 RTALPR----SATVGRVPNRKPPPPGRANPDARKETAQDKPTGRVPQSTSARAAGLPRSA 522
+ A P + + G P P P R P A + TA P ST A A P+
Sbjct: 110 QPAAPSAPAPAPSQGPRPTPGPKPAPRPAPAAPEFTA--PPAAPAAPSTPAPAPSGPKPG 167
Query: 523 NTNSRSAPK 549
APK
Sbjct: 168 GARP-GAPK 175
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.317 0.128 0.382
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 76,303,920
Number of Sequences: 369166
Number of extensions: 1657242
Number of successful extensions: 8125
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6656
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7899
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 5218718490
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)