Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_020_C18
(795 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O70351|HCD2_RAT 3-hydroxyacyl-CoA dehydrogenase type II ... 256 5e-68
sp|O18404|HCD2_DROME 3-hydroxyacyl-CoA dehydrogenase type I... 254 2e-67
sp|O02691|HCD2_BOVIN 3-hydroxyacyl-CoA dehydrogenase type I... 252 9e-67
sp|Q99714|HCD2_HUMAN 3-hydroxyacyl-CoA dehydrogenase type I... 250 4e-66
sp|O08756|HCD2_MOUSE 3-hydroxyacyl-CoA dehydrogenase type I... 248 2e-65
sp|Q9X248|FABG_THEMA 3-oxoacyl-[acyl-carrier-protein] reduc... 95 3e-19
sp|P99120|BUTA_STAAN Acetoin(diacetyl) reductase (Acetoin d... 88 2e-17
sp|P66781|YD50_MYCTU Putative oxidoreductase Rv1350/MT1393 ... 85 2e-16
sp|P46331|YXBG_BACSU Hypothetical oxidoreductase yxbG 85 2e-16
sp|Q92506|DHB8_HUMAN Estradiol 17-beta-dehydrogenase 8 (17-... 85 3e-16
>sp|O70351|HCD2_RAT 3-hydroxyacyl-CoA dehydrogenase type II (Type II HADH) (Endoplasmic
reticulum-associated amyloid beta-peptide binding
protein)
Length = 261
Score = 256 bits (654), Expect = 5e-68
Identities = 125/236 (52%), Positives = 166/236 (70%), Gaps = 6/236 (2%)
Frame = +3
Query: 45 TVERLTKQGCKVAICDIQ------EPKRLPANSYYIKTDVSCANDVENAITQIKSEAKKL 206
T +RL QG + D+ E K+L N + +V+ +V+ A+T K + ++
Sbjct: 26 TAKRLVGQGATAVLLDVPNSEGETEAKKLGGNCIFAPANVTSEKEVQAALTLAKEKFGRI 85
Query: 207 DILVNCAGIGVAVKVYNRKKNFPHPLDTFERIVKVNAAGTFNMIRLCCSMFNSNEPNEFN 386
D+ VNCAGI VA+K Y+ KKN H L+ F+R++ VN GTFN+IRL + NEP++
Sbjct: 86 DVAVNCAGIAVAIKTYHEKKNQVHTLEDFQRVINVNLIGTFNVIRLVAGVMGQNEPDQGG 145
Query: 387 QRGIVINTSSIAAFEGQCGQSAYSASQGAIVAMTLPLARDLVAMGVRVMTIAPGIFLTPL 566
QRG++INT+S+AAFEGQ GQ+AYSAS+G IV MTLP+ARDL +G+RV+TIAPG+F TPL
Sbjct: 146 QRGVIINTASVAAFEGQVGQAAYSASKGGIVGMTLPIARDLAPIGIRVVTIAPGLFATPL 205
Query: 567 LENVPDSARLNLSKIVPFPSRLGHPDEFAHLVQSIIENPMLNGEVIRIDGALRMMP 734
L +PD R L+ VPFPSRLG P E+AHLVQ +IENP LNGEVIR+DGA+RM P
Sbjct: 206 LTTLPDKVRNFLASQVPFPSRLGDPAEYAHLVQMVIENPFLNGEVIRLDGAIRMQP 261
>sp|O18404|HCD2_DROME 3-hydroxyacyl-CoA dehydrogenase type II (Type II HADH) (Scully
protein)
Length = 255
Score = 254 bits (649), Expect = 2e-67
Identities = 122/236 (51%), Positives = 163/236 (69%), Gaps = 6/236 (2%)
Frame = +3
Query: 45 TVERLTKQGCKVAICDI------QEPKRLPANSYYIKTDVSCANDVENAITQIKSEAKKL 206
T ERL KQG V + D+ + K L ++ DV+ DV A+ K + +L
Sbjct: 20 TAERLAKQGASVILADLPSSKGNEVAKELGDKVVFVPVDVTSEKDVSAALQTAKDKFGRL 79
Query: 207 DILVNCAGIGVAVKVYNRKKNFPHPLDTFERIVKVNAAGTFNMIRLCCSMFNSNEPNEFN 386
D+ VNCAG AVK +N KN H L+ F+R++ +N GTFN+IRL + +NEPN+
Sbjct: 80 DLTVNCAGTATAVKTFNFNKNVAHRLEDFQRVININTVGTFNVIRLSAGLMGANEPNQDG 139
Query: 387 QRGIVINTSSIAAFEGQCGQSAYSASQGAIVAMTLPLARDLVAMGVRVMTIAPGIFLTPL 566
QRG+++NT+S+AAF+GQ GQ+AYSAS+ A+V MTLP+ARDL G+R+ TIAPG+F TP+
Sbjct: 140 QRGVIVNTASVAAFDGQIGQAAYSASKAAVVGMTLPIARDLSTQGIRICTIAPGLFNTPM 199
Query: 567 LENVPDSARLNLSKIVPFPSRLGHPDEFAHLVQSIIENPMLNGEVIRIDGALRMMP 734
L +P+ R L+K +PFP RLG P E+AHLVQ+I ENP+LNGEVIRIDGALRMMP
Sbjct: 200 LAALPEKVRTFLAKSIPFPQRLGEPSEYAHLVQAIYENPLLNGEVIRIDGALRMMP 255
>sp|O02691|HCD2_BOVIN 3-hydroxyacyl-CoA dehydrogenase type II (Type II HADH)
Length = 261
Score = 252 bits (643), Expect = 9e-67
Identities = 127/236 (53%), Positives = 164/236 (69%), Gaps = 6/236 (2%)
Frame = +3
Query: 45 TVERLTKQGCKVAICDIQ------EPKRLPANSYYIKTDVSCANDVENAITQIKSEAKKL 206
T ERL QG + D+ + K+L + + DV+ DV+ A+T + + ++
Sbjct: 26 TAERLVGQGATAVLLDLPNSDGETQAKKLGKSCAFAPADVTSEKDVQAALTLAREKFGRV 85
Query: 207 DILVNCAGIGVAVKVYNRKKNFPHPLDTFERIVKVNAAGTFNMIRLCCSMFNSNEPNEFN 386
D+ VNCAGI VA K YN KK+ H L+ F+R++ VN GTFN+IRL NEP++
Sbjct: 86 DVAVNCAGIAVASKTYNLKKSQAHTLEDFQRVINVNLIGTFNVIRLVAGEMGQNEPDQGG 145
Query: 387 QRGIVINTSSIAAFEGQCGQSAYSASQGAIVAMTLPLARDLVAMGVRVMTIAPGIFLTPL 566
QRG++INT+S+AAFEGQ GQ+AYSAS+G IV MTLP+ARDL MG+RVMTIAPG+F TPL
Sbjct: 146 QRGVIINTASVAAFEGQVGQAAYSASKGGIVGMTLPIARDLAPMGIRVMTIAPGLFGTPL 205
Query: 567 LENVPDSARLNLSKIVPFPSRLGHPDEFAHLVQSIIENPMLNGEVIRIDGALRMMP 734
L +PD R L+ VPFPSRLG P E+AHLVQ+IIEN LNGEVIR+DGA+RM P
Sbjct: 206 LTTLPDKVRNFLASQVPFPSRLGDPAEYAHLVQAIIENSFLNGEVIRLDGAIRMQP 261
>sp|Q99714|HCD2_HUMAN 3-hydroxyacyl-CoA dehydrogenase type II (Type II HADH) (Endoplasmic
reticulum-associated amyloid beta-peptide binding
protein) (Short-chain type dehydrogenase/reductase
XH98G2)
Length = 261
Score = 250 bits (638), Expect = 4e-66
Identities = 126/236 (53%), Positives = 163/236 (69%), Gaps = 6/236 (2%)
Frame = +3
Query: 45 TVERLTKQGCKVAICDIQ------EPKRLPANSYYIKTDVSCANDVENAITQIKSEAKKL 206
T ERL QG + D+ + K+L N + DV+ DV+ A+ K + ++
Sbjct: 26 TAERLVGQGASAVLLDLPNSGGEAQAKKLGNNCVFAPADVTSEKDVQTALALAKGKFGRV 85
Query: 207 DILVNCAGIGVAVKVYNRKKNFPHPLDTFERIVKVNAAGTFNMIRLCCSMFNSNEPNEFN 386
D+ VNCAGI VA K YN KK H L+ F+R++ VN GTFN+IRL NEP++
Sbjct: 86 DVAVNCAGIAVASKTYNLKKGQTHTLEDFQRVLDVNLMGTFNVIRLVAGEMGQNEPDQGG 145
Query: 387 QRGIVINTSSIAAFEGQCGQSAYSASQGAIVAMTLPLARDLVAMGVRVMTIAPGIFLTPL 566
QRG++INT+S+AAFEGQ GQ+AYSAS+G IV MTLP+ARDL +G+RVMTIAPG+F TPL
Sbjct: 146 QRGVIINTASVAAFEGQVGQAAYSASKGGIVGMTLPIARDLAPIGIRVMTIAPGLFGTPL 205
Query: 567 LENVPDSARLNLSKIVPFPSRLGHPDEFAHLVQSIIENPMLNGEVIRIDGALRMMP 734
L ++P+ L+ VPFPSRLG P E+AHLVQ+IIENP LNGEVIR+DGA+RM P
Sbjct: 206 LTSLPEKVCNFLASQVPFPSRLGDPAEYAHLVQAIIENPFLNGEVIRLDGAIRMQP 261
>sp|O08756|HCD2_MOUSE 3-hydroxyacyl-CoA dehydrogenase type II (Type II HADH) (Endoplasmic
reticulum-associated amyloid beta-peptide binding
protein)
Length = 261
Score = 248 bits (632), Expect = 2e-65
Identities = 121/236 (51%), Positives = 165/236 (69%), Gaps = 6/236 (2%)
Frame = +3
Query: 45 TVERLTKQGCKVAICDI------QEPKRLPANSYYIKTDVSCANDVENAITQIKSEAKKL 206
T +RL QG + D+ + K+L + + +V+ +++ A+T K + ++
Sbjct: 26 TAKRLVGQGATAVLLDVPDSEGESQAKKLGESCIFAPANVTSEKEIQAALTLAKEKFGRI 85
Query: 207 DILVNCAGIGVAVKVYNRKKNFPHPLDTFERIVKVNAAGTFNMIRLCCSMFNSNEPNEFN 386
D+ VNCAGI VA+K Y++KKN H L+ F+R++ VN GTFN+IRL NEP++
Sbjct: 86 DVAVNCAGIAVAIKTYHQKKNKIHTLEDFQRVINVNLIGTFNVIRLVAGEMGQNEPDQGG 145
Query: 387 QRGIVINTSSIAAFEGQCGQSAYSASQGAIVAMTLPLARDLVAMGVRVMTIAPGIFLTPL 566
QRG++INT+S+AAFEGQ GQ+AYSAS+G I MTLP+ARDL G+RV+TIAPG+F TPL
Sbjct: 146 QRGVIINTASVAAFEGQVGQAAYSASKGGIDGMTLPIARDLAPTGIRVVTIAPGLFATPL 205
Query: 567 LENVPDSARLNLSKIVPFPSRLGHPDEFAHLVQSIIENPMLNGEVIRIDGALRMMP 734
L +P+ R L+ VPFPSRLG P E+AHLVQ+IIENP LNGEVIR+DGA+RM P
Sbjct: 206 LTTLPEKVRNFLASQVPFPSRLGDPAEYAHLVQTIIENPFLNGEVIRLDGAIRMQP 261
>sp|Q9X248|FABG_THEMA 3-oxoacyl-[acyl-carrier-protein] reductase (3-ketoacyl-acyl carrier
protein reductase)
Length = 246
Score = 94.7 bits (234), Expect = 3e-19
Identities = 59/213 (27%), Positives = 107/213 (50%), Gaps = 3/213 (1%)
Frame = +3
Query: 93 IQEPKRLPANSYYIKTDVSCANDVENAITQIKSEAKKLDILVNCAGIGVAVKVYNRKKNF 272
++E + LP +V+ + ++ + ++ + ++D+LVN AGI + K+
Sbjct: 46 VKEAEGLPGKVDPYVLNVTDRDQIKEVVEKVVQKYGRIDVLVNNAGITRDALLVRMKE-- 103
Query: 273 PHPLDTFERIVKVNAAGTFNMIRLCCSMFNSNEPNEFNQR-GIVINTSSIAAFEGQCGQS 449
+ ++ ++ VN G FN+ ++ P QR G ++N SS+ G GQ+
Sbjct: 104 ----EDWDAVINVNLKGVFNVTQMVV-------PYMIKQRNGSIVNVSSVVGIYGNPGQT 152
Query: 450 AYSASQGAIVAMTLPLARDLVAMGVRVMTIAPGIFLTPLLENVPDSARLNLSKIVPFPSR 629
Y+AS+ ++ MT A++L +RV +APG TP+ E +P+ AR +P R
Sbjct: 153 NYAASKAGVIGMTKTWAKELAGRNIRVNAVAPGFIETPMTEKLPEKARETALSRIPL-GR 211
Query: 630 LGHPDEFAHLVQSII--ENPMLNGEVIRIDGAL 722
G P+E A ++ + E+ + G+VI IDG L
Sbjct: 212 FGKPEEVAQVILFLASDESSYVTGQVIGIDGGL 244
>sp|P99120|BUTA_STAAN Acetoin(diacetyl) reductase (Acetoin dehydrogenase) (AR)
(Meso-2,3-butanediol dehydrogenase)
sp|P66776|BUTA_STAAW Acetoin(diacetyl) reductase (Acetoin dehydrogenase) (AR)
(Meso-2,3-butanediol dehydrogenase)
sp|P66775|BUTA_STAAM Acetoin(diacetyl) reductase (Acetoin dehydrogenase) (AR)
(Meso-2,3-butanediol dehydrogenase)
sp|Q6GKH9|BUTA_STAAR Acetoin(diacetyl) reductase (Acetoin dehydrogenase) (AR)
(Meso-2,3-butanediol dehydrogenase)
sp|Q6GCZ8|BUTA_STAAS Acetoin(diacetyl) reductase (Acetoin dehydrogenase) (AR)
(Meso-2,3-butanediol dehydrogenase)
Length = 258
Score = 88.2 bits (217), Expect = 2e-17
Identities = 70/251 (27%), Positives = 114/251 (45%), Gaps = 26/251 (10%)
Frame = +3
Query: 51 ERLTKQGCKVAICDIQEPKRLPA---------NSYYIKTDVSCANDVENAITQIKSEAKK 203
ERL + G KVA+ D E A + IK DVS +DV NA+ Q ++
Sbjct: 22 ERLVEDGFKVAVVDFNEEGAKAAALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGD 81
Query: 204 LDILVNCAGIGVAVKVYNRKKNFPHPLDT-----FERIVKVNAAGTFNMIRLCCSMFNSN 368
++VN AG+G P+DT F+ + VN AG I+ F
Sbjct: 82 FHVMVNNAGLGPTT-----------PIDTITEEQFKTVYGVNVAGVLWGIQAAHEQFK-- 128
Query: 369 EPNEFNQRGIVINTSSIAAFEGQCGQSAYSASQGAIVAMTLPLARDLVAMGVRVMTIAPG 548
+FN G +IN +S A EG G S Y +++ A+ +T A+DL + G+ V APG
Sbjct: 129 ---KFNHGGKIINATSQAGVEGNPGLSLYCSTKFAVRGLTQVAAQDLASEGITVNAFAPG 185
Query: 549 IFLTPLLENVPDSARLNLSKIVPFP----------SRLGHPDEFAHLVQSII--ENPMLN 692
I TP++E++ + K + R+ P++ +++V + ++ +
Sbjct: 186 IVQTPMMESIAVATAEEAGKPEAWGWEQFTSQIALGRVSQPEDVSNVVSFLAGKDSDYIT 245
Query: 693 GEVIRIDGALR 725
G+ I +DG +R
Sbjct: 246 GQTIIVDGGMR 256
>sp|P66781|YD50_MYCTU Putative oxidoreductase Rv1350/MT1393
sp|P66782|YD85_MYCBO Putative oxidoreductase Mb1385
Length = 247
Score = 85.1 bits (209), Expect = 2e-16
Identities = 64/233 (27%), Positives = 113/233 (48%), Gaps = 11/233 (4%)
Frame = +3
Query: 51 ERLTKQGCKVAICDIQ------EPKRLPAN--SYYIKTDVSCANDVENAITQIKSEAKKL 206
+R +G +V + D+ KRL + + ++ DV+ A+DV+ I L
Sbjct: 25 QRFVAEGARVVLGDVNLEATEVAAKRLGGDDVALAVRCDVTQADDVDILIRTAVERFGGL 84
Query: 207 DILVNCAGIGVAVKVYNRKKNFPHPLDTFERIVKVNAAGTFNMIRLCCSMFNSNEPNEFN 386
D++VN AGI + + + F++++ V+ GT+N RL ++ +
Sbjct: 85 DVMVNNAGITRDATMRTMTE------EQFDQVIAVHLKGTWNGTRLAAAIMRERK----- 133
Query: 387 QRGIVINTSSIAAFEGQCGQSAYSASQGAIVAMTLPLARDLVAMGVRVMTIAPGIFLTPL 566
RG ++N SS++ G GQ+ YSA++ IV MT A++L +G+RV IAPG+ + +
Sbjct: 134 -RGAIVNMSSVSGKVGMVGQTNYSAAKAGIVGMTKAAAKELAHLGIRVNAIAPGLIRSAM 192
Query: 567 LENVPDSARLNLSKIVPFP-SRLGHPDEFAHLVQSIIE--NPMLNGEVIRIDG 716
E +P R+ K+ P R G P E A + + + + G V+ + G
Sbjct: 193 TEAMPQ--RIWDQKLAEVPMGRAGEPSEVASVAVFLASDLSSYMTGTVLDVTG 243
>sp|P46331|YXBG_BACSU Hypothetical oxidoreductase yxbG
Length = 273
Score = 85.1 bits (209), Expect = 2e-16
Identities = 77/267 (28%), Positives = 117/267 (43%), Gaps = 21/267 (7%)
Frame = +3
Query: 45 TVERLTKQGCKVAICDIQEPKRLP-----------ANSYYIKTDVSCANDVENAITQIKS 191
T E +G +V I DI + + A S+++ DVS N V+ QIK
Sbjct: 22 TAEVFANEGARVIIGDINKDQMEETVDAIRKNGGQAESFHL--DVSDENSVKAFADQIKD 79
Query: 192 EAKKLDILVNCAGIGVAVKVYNRKKNFPHPLDTFERIVKVNAAGTFNMIRLCCSMFNSNE 371
+DIL N AG+ K +P+D F+RI+ V+ GTF CS +
Sbjct: 80 ACGTIDILFNNAGVD-----QEGGKVHEYPVDLFDRIIAVDLRGTF-----LCSKYLI-- 127
Query: 372 PNEFNQRGIVINTSSIAAFEGQCGQSAYSASQGAIVAMTLPLARDLVAMGVRVMTIAPGI 551
P G +INTSS++ +S Y+A++G I +T +A D G+RV +I+PG
Sbjct: 128 PLMLENGGSIINTSSMSGRAADLDRSGYNAAKGGITNLTKAMAIDYARNGIRVNSISPGT 187
Query: 552 FLTPLLENVPDSARLNLSK--------IVPFPSRLGHPDEFAHLVQSII--ENPMLNGEV 701
TPL++ + + + + I P RLG P E A + + ++ + GE
Sbjct: 188 IETPLIDKLAGTKEQEMGEQFREANKWITPL-GRLGQPKEMATVALFLASDDSSYVTGED 246
Query: 702 IRIDGALRMMP*LLFWYNKTCIRFKMK 782
I DG + W K I K K
Sbjct: 247 ITADGGIMAYT----WPGKMLIEEKWK 269
>sp|Q92506|DHB8_HUMAN Estradiol 17-beta-dehydrogenase 8 (17-beta-HSD 8)
(17-beta-hydroxysteroid dehydrogenase 8) (Protein Ke6)
(Ke-6)
Length = 261
Score = 84.7 bits (208), Expect = 3e-16
Identities = 65/244 (26%), Positives = 114/244 (46%), Gaps = 19/244 (7%)
Frame = +3
Query: 54 RLTKQGCKVAICDI-----QEPKRL-----------PANSYYIKTDVSCANDVENAITQI 185
RL +G VA CD+ QE RL N + DVS A + Q+
Sbjct: 30 RLAGEGATVAACDLDRAAAQETVRLLGGPGSKEGPPRGNHAAFQADVSEARAARCLLEQV 89
Query: 186 KS-EAKKLDILVNCAGIGVAVKVYNRKKNFPHPLDTFERIVKVNAAGTFNMIRLCCSMFN 362
++ ++ ++V+CAGI + + + D +++++ VN GTF + +
Sbjct: 90 QACFSRPPSVVVSCAGITQDEFLLHMSE------DDWDKVIAVNLKGTFLVTQAAAQALV 143
Query: 363 SNEPNEFNQRGIVINTSSIAAFEGQCGQSAYSASQGAIVAMTLPLARDLVAMGVRVMTIA 542
SN RG +IN SSI G GQ+ Y+AS+ ++ +T AR+L G+R ++
Sbjct: 144 SN-----GCRGSIINISSIVGKVGNVGQTNYAASKAGVIGLTQTAARELGRHGIRCNSVL 198
Query: 543 PGIFLTPLLENVPDSARLNLSKIVPFPSRLGHPDEFAHLVQSII--ENPMLNGEVIRIDG 716
PG TP+ + VP +++++P LG P++ A +V + ++ + G + + G
Sbjct: 199 PGFIATPMTQKVPQKVVDKITEMIPM-GHLGDPEDVADVVAFLASEDSGYITGTSVEVTG 257
Query: 717 ALRM 728
L M
Sbjct: 258 GLFM 261
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 84,801,627
Number of Sequences: 369166
Number of extensions: 1710088
Number of successful extensions: 5309
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5033
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5176
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7473924075
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)