Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_020_C14
(894 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q10776|FAD11_MYCTU Putative fatty-acid--CoA ligase fadD1... 105 1e-22
sp|P44446|LCFH_HAEIN Putative long-chain-fatty-acid--CoA li... 104 4e-22
sp|Q9ULC5|ACSL5_HUMAN Long-chain-fatty-acid--CoA ligase 5 (... 92 1e-18
sp|O60135|LCF1_SCHPO Long-chain-fatty-acid--CoA ligase 1 (L... 84 5e-16
sp|O88813|ACSL5_RAT Long-chain-fatty-acid--CoA ligase 5 (Lo... 82 2e-15
sp|Q8JZR0|ACSL5_MOUSE Long-chain-fatty-acid--CoA ligase 5 (... 81 3e-15
sp|P41216|ACSL1_MOUSE Long-chain-fatty-acid--CoA ligase 1 (... 81 3e-15
sp|Q63151|ACSL3_RAT Long-chain-fatty-acid--CoA ligase 3 (Lo... 79 1e-14
sp|P18163|ACSL1_RAT Long-chain-fatty-acid--CoA ligase 1 (Lo... 78 3e-14
sp|Q9CZW4|ACSL3_MOUSE Long-chain-fatty-acid--CoA ligase 3 (... 78 4e-14
>sp|Q10776|FAD11_MYCTU Putative fatty-acid--CoA ligase fadD11 (Acyl-CoA synthetase)
Length = 571
Score = 105 bits (263), Expect = 1e-22
Identities = 74/216 (34%), Positives = 115/216 (53%), Gaps = 5/216 (2%)
Frame = +2
Query: 242 PNECCSLIYTSGTTGNPKGVMMSHDNLIWTASCLDYHLKFRSSKEVLISYLPLSHIAAQM 421
P + +LIYTSGTTGNPKGV M+H NL++ +D L R V S+LP +HIA +M
Sbjct: 130 PEDIVTLIYTSGTTGNPKGVEMTHANLLFEGYAIDEVLGIRFGDRV-TSFLPSAHIADRM 188
Query: 422 VDIIVPLICQATVYFAQPDALKGTLGNTLREVHPTYFIGVPRVFEKIMEQMK-EMASQLS 598
+ + + T A DA T+ L +V PT + VPRV+EK+ ++ +A +
Sbjct: 189 TGLYLQEMF-GTQVTAVADA--RTIAAALPDVRPTVWGAVPRVWEKLKAGIEFTVARETD 245
Query: 599 SLKKYISSWAKDIGYRTTLAKMSGE--SGPFGYQLAKC--ILFNKVHKALGLDRCVAIIS 766
+K+ +WA + + A ++GE S + AK ++ +K+ + LG +S
Sbjct: 246 EMKRQALAWAMSVAGKRANALLAGESMSDQLVAEWAKADELVLSKLRERLGFGELRWALS 305
Query: 767 SAAPLSHDALVYFGSLDLVICEIYGMSECSGPHTVS 874
AAP+ + L +F + + I EI+GMSE S T S
Sbjct: 306 GAAPIPKETLAFFAGIGIPIAEIWGMSELSCVATAS 341
>sp|P44446|LCFH_HAEIN Putative long-chain-fatty-acid--CoA ligase (Long-chain acyl-CoA
synthetase) (LACS)
Length = 607
Score = 104 bits (259), Expect = 4e-22
Identities = 77/300 (25%), Positives = 143/300 (47%), Gaps = 4/300 (1%)
Frame = +2
Query: 2 YIINNCNCAVAVVGSSELFKKYMSIADKCPTLKAIIYYGEKVEESAKNNSKLLIYDWTEF 181
+I+N+ + + VG E + + + IA CP L+ I+ ++ + + L W F
Sbjct: 108 FILNHADVKILFVGDQEQYDQTLEIAHHCPKLQKIV----AMKSTIQLQQDPLSCTWESF 163
Query: 182 MNLGNDINDDLLIKRLSNLIPNECCSLIYTSGTTGNPKGVMMSHDNLIWTASCLDYHLKF 361
+ G++ D L +RL+ ++ ++IYTSGTTG PKGVM+ + NL D L
Sbjct: 164 IKTGSNAQQDELTQRLNQKQLSDLFTIIYTSGTTGEPKGVMLDYANLAHQLETHDLSLNV 223
Query: 362 RSSKEVLISYLPLSHIAAQMVDIIVPLICQATVYFAQPDALKGTLGNTLREVHPTYFIGV 541
+ +++ +S+LP SHI + + Y + ++ + L E+ PT V
Sbjct: 224 -TDQDISLSFLPFSHIFERAWAAYILHRGAILCYLEDTNQVR----SALTEIRPTLMCAV 278
Query: 542 PRVFEKIMEQMKEMASQLSSLKKYISSWAKDIGYRTTLAKMSGESGPF----GYQLAKCI 709
PR +EKI + + + L++ + WA +G + + + ++ PF + LA +
Sbjct: 279 PRFYEKIYAAVLDKVQKAPKLRQIMFHWAISVGQKYFDLRANNKAIPFLLKKQFALADKL 338
Query: 710 LFNKVHKALGLDRCVAIISSAAPLSHDALVYFGSLDLVICEIYGMSECSGPHTVSLTNSF 889
+ +K+ + LG R + A L ++F ++ + I YGM+E + TVS + F
Sbjct: 339 VLSKLRQLLG-GRIKMMPCGGAKLEPAIGLFFHAIGINIKLGYGMTETTA--TVSCWHDF 395
>sp|Q9ULC5|ACSL5_HUMAN Long-chain-fatty-acid--CoA ligase 5 (Long-chain acyl-CoA synthetase
5) (LACS 5)
Length = 683
Score = 92.4 bits (228), Expect = 1e-18
Identities = 84/304 (27%), Positives = 149/304 (49%), Gaps = 12/304 (3%)
Frame = +2
Query: 2 YIINNCNCAVAVVGSSELFKKYMSIADK--CPTLKAIIYYGEKVEESAKNNSK-----LL 160
+I+N + A+ + + + + +K P+LK II ++ + K L
Sbjct: 173 HIVNKADIAMVICDTPQKALVLIGNVEKGFTPSLKVIILMDPFDDDLKQRGEKSGIEILS 232
Query: 161 IYDWTEFMNLGNDINDDLLIKRLSNLIPNECCSLIYTSGTTGNPKGVMMSHDNLIWTAS- 337
+YD NLG + ++ P + + +TSGTTG+PKG M++H N++ A+
Sbjct: 233 LYDAE---NLGKEH-----FRKPVPPSPEDLSVICFTSGTTGDPKGAMITHQNIVSNAAA 284
Query: 338 ---CLDYHLKFRSSKEVLISYLPLSHIAAQMVDIIVPLICQATVYFAQPDALKGTLGNTL 508
C++ H + +V ISYLPL+H+ ++V +V C A V F Q D L + +
Sbjct: 285 FLKCVE-HAYEPTPDDVAISYLPLAHMFERIVQAVV-YSCGARVGFFQGDI--RLLADDM 340
Query: 509 REVHPTYFIGVPRVFEKIMEQMKEMASQLSSLKKYISSWAKDIGYRTTLAKMSGESGPFG 688
+ + PT F VPR+ +I ++++ A + LKK++ A ++ L K F
Sbjct: 341 KTLKPTLFPAVPRLLNRIYDKVQNEAK--TPLKKFLLKLAVSSKFK-ELQKGIIRHDSFW 397
Query: 689 YQLAKCILFNKVHKALGLDRCVAIISSAAPLSHDALVYF-GSLDLVICEIYGMSECSGPH 865
+L +F K+ +LG R I++ AAP+S + +F ++ + E YG +EC+G
Sbjct: 398 DKL----IFAKIQDSLG-GRVRVIVTGAAPMSTSVMTFFRAAMGCQVYEAYGQTECTGGC 452
Query: 866 TVSL 877
T +L
Sbjct: 453 TFTL 456
>sp|O60135|LCF1_SCHPO Long-chain-fatty-acid--CoA ligase 1 (Long-chain acyl-CoA synthetase
1) (Fatty acid activator 1)
Length = 676
Score = 84.0 bits (206), Expect = 5e-16
Identities = 75/282 (26%), Positives = 129/282 (45%), Gaps = 14/282 (4%)
Frame = +2
Query: 53 LFKKYMSIADKCPTLKAIIYYGEKVEESAKN----NSKLLIYDWTEFMNLGNDINDDLLI 220
L K ++ + +L+ IIY + EE K L + + +F + + D
Sbjct: 178 LLKTLVNPLKEIESLEVIIYRNDAKEEDIKTIQEIRPNLKLIKFADFEKMSPPVEPD--- 234
Query: 221 KRLSNLIPNECCSLIYTSGTTGNPKGVMMSHDNLIWTASCLDYHLKFRSSKEVLISYLPL 400
P E C ++YTSG+TG PKGV++SH N++ + + H+ +SK+ L++YLPL
Sbjct: 235 ----PPSPEEICCIMYTSGSTGLPKGVILSHKNMVAIVTAIVKHVPEVTSKDYLLAYLPL 290
Query: 401 SHIAAQMVDIIVPLICQA---TVYFAQPDALKGT----LGNTLREVHPTYFIGVPRVFEK 559
+HI ++ IC A T+ +A L T + PT +GVP V+E
Sbjct: 291 AHI----LEFAFENICLAWGGTIGYANVRTLVDTNCRNCKGDINTFRPTIMVGVPAVWEM 346
Query: 560 IMEQMKEMASQLSSLKKYISSWAKDIGYRTTLAKMSGESGPFGYQLAKCILFNKVHKALG 739
+ + + + S++K+ + A T AK+ + P G + +FNK+
Sbjct: 347 VRKGIMSKLNAASAVKRSVFWTA-----YYTKAKLMRHNLP-GSCVLDTAVFNKIRSMGT 400
Query: 740 LDRCVAIISSAAPLSHDALVYFGSLDLVICEI---YGMSECS 856
R +S + LS D + L +V+C + YG++E S
Sbjct: 401 GGRLRYTLSGGSALSPDTKRF---LSIVLCPMLIGYGLTEIS 439
>sp|O88813|ACSL5_RAT Long-chain-fatty-acid--CoA ligase 5 (Long-chain acyl-CoA synthetase
5) (LACS 5)
Length = 683
Score = 82.4 bits (202), Expect = 2e-15
Identities = 76/299 (25%), Positives = 144/299 (48%), Gaps = 14/299 (4%)
Frame = +2
Query: 2 YIINNCNCAVAVVGSSELFKKYMSIADK--CPTLKAIIYYGEKVEESAKNNSK--LLIYD 169
Y+IN + +V + + + + +K P LK +I ++ K K + +
Sbjct: 173 YVINRADISVVICDTPQKATMLIENVEKDLTPGLKTVILMDPFDDDLMKRGEKCGIEMLS 232
Query: 170 WTEFMNLGNDINDDLLIKRLSNLIPNECCSLIYTSGTTGNPKGVMMSHDNLIWTASCLDY 349
+ NLG + K+ P + + +TSGTTG+PKG M++H N++ S +
Sbjct: 233 LHDAENLGKEN-----FKKPMPPNPEDLSVICFTSGTTGDPKGAMLTHQNIV---SNMAA 284
Query: 350 HLKF------RSSKEVLISYLPLSHIAAQMVDIIVPLICQATVYFAQPDALKGTLGNTLR 511
LKF + ++V ISYLPL+H+ ++V ++ C + F Q D L + ++
Sbjct: 285 FLKFLEPIFQPTPEDVTISYLPLAHMFERLVQGVI-FSCGGKIGFFQGDI--RLLPDDMK 341
Query: 512 EVHPTYFIGVPRVFEKIMEQMKEMASQLSSLKKYISSWA---KDIGYRTTLAKMSGESGP 682
+ PT F VPR+ ++ ++++ A + LKK++ + A K R + + +
Sbjct: 342 ALKPTVFPTVPRLLNRVYDKVQNEAK--TPLKKFLLNLAIISKFNEVRNGIIRRN----- 394
Query: 683 FGYQLAKCILFNKVHKALGLDRCVAIISSAAPLSHDALVYF-GSLDLVICEIYGMSECS 856
L ++F+K+ +LG + +I+ AAP+S L +F ++ + E YG +EC+
Sbjct: 395 ---SLWDKLVFSKIQSSLG-GKVRLMITGAAPISTPVLTFFRAAMGCWVFEAYGQTECT 449
>sp|Q8JZR0|ACSL5_MOUSE Long-chain-fatty-acid--CoA ligase 5 (Long-chain acyl-CoA synthetase
5) (LACS 5)
Length = 683
Score = 81.3 bits (199), Expect = 3e-15
Identities = 62/213 (29%), Positives = 112/213 (52%), Gaps = 7/213 (3%)
Frame = +2
Query: 242 PNECCSLIYTSGTTGNPKGVMMSHDNLIWTASCLDYHLKF------RSSKEVLISYLPLS 403
P + + +TSGTTG+PKG M++H+N++ S + LKF +S +V ISYLPL+
Sbjct: 252 PEDLSVICFTSGTTGDPKGAMLTHENVV---SNMAAFLKFLEPIFQPTSDDVTISYLPLA 308
Query: 404 HIAAQMVDIIVPLICQATVYFAQPDALKGTLGNTLREVHPTYFIGVPRVFEKIMEQMKEM 583
H+ ++V I+ C + F Q D L + ++ + PT F VPR+ ++ ++++
Sbjct: 309 HMFERLVQGIL-FSCGGKIGFFQGDI--RLLPDDMKALKPTVFPTVPRLLNRVYDKVQNE 365
Query: 584 ASQLSSLKKYISSWAKDIGYRTTLAKMSGESGPFGYQLAKCILFNKVHKALGLDRCVAII 763
A + LKK++ + A + + L ++F+K+ +LG + +I
Sbjct: 366 AK--TPLKKFLLNLAIISKFNEVKNGIIRRD-----SLWDKLVFSKIQGSLG-GKVRLMI 417
Query: 764 SSAAPLSHDALVYF-GSLDLVICEIYGMSECSG 859
+ AAP+S L +F ++ + E YG +EC+G
Sbjct: 418 TGAAPISTPVLTFFRAAMGCWVFEAYGQTECTG 450
>sp|P41216|ACSL1_MOUSE Long-chain-fatty-acid--CoA ligase 1 (Long-chain acyl-CoA synthetase
1) (LACS 1)
Length = 699
Score = 81.3 bits (199), Expect = 3e-15
Identities = 81/306 (26%), Positives = 144/306 (47%), Gaps = 14/306 (4%)
Frame = +2
Query: 2 YIINNCNCAVAVVGSSELFKKYMSIADK--CPTLKAII----YYGEKVEESAKNNSKLLI 163
YI+N +V E K + + P LK I+ Y + VE K +++
Sbjct: 189 YIVNKAELSVIFADKPEKAKLLLEGVENKLTPCLKIIVIMDSYGSDLVERGKKCGVEIIS 248
Query: 164 YDWTEFMNLGNDINDDLLIKRLSNLIPNECCSLIYTSGTTGNPKGVMMSHDNLIWTASCL 343
E + N + P + + +TSGTTGNPKG M++H N+I S
Sbjct: 249 LKALEDLGRVNRVKP-------KPPEPEDLAIICFTSGTTGNPKGAMITHQNIINDCSGF 301
Query: 344 DYHLK---FRSSKEVLISYLPLSHIAAQMVDIIVPLICQ-ATVYFAQPDALKGTLGNTLR 511
+ S+ +VLIS+LPL+H+ +V+ + ++C A + F Q D L + L+
Sbjct: 302 IKATESAFIASTDDVLISFLPLAHMFETVVECV--MLCHGAKIGFFQGDI--RLLMDDLK 357
Query: 512 EVHPTYFIGVPRVFEKIMEQMKEMASQLSSLKKYISSWA---KDIGYRTTLAKMSGESGP 682
+ PT F VPR+ ++ +++ A+ +SLK+++ +A K+ R+ + + +
Sbjct: 358 VLQPTIFPVVPRLLNRMFDRIFGQAN--TSLKRWLLDFASKRKEADVRSGIVRNN----- 410
Query: 683 FGYQLAKCILFNKVHKALGLDRCVAIISSAAPLSHDALVYF-GSLDLVICEIYGMSECSG 859
L ++F+K+ +LG + +I+ AAP+S L + +L E YG +EC+
Sbjct: 411 ---SLWDKLIFHKIQSSLG-GKVRLMITGAAPVSATVLTFLRTALGCQFYEGYGQTECTA 466
Query: 860 PHTVSL 877
+SL
Sbjct: 467 GCCLSL 472
>sp|Q63151|ACSL3_RAT Long-chain-fatty-acid--CoA ligase 3 (Long-chain acyl-CoA synthetase
3) (LACS 3) (Brain acyl-CoA synthtase II)
Length = 720
Score = 79.3 bits (194), Expect = 1e-14
Identities = 71/296 (23%), Positives = 137/296 (46%), Gaps = 16/296 (5%)
Frame = +2
Query: 35 VVGSSELFK-KYMSIADKCPTLKAIIYYGEKVEESAKNNSKLLIYDWTEFMNLGNDINDD 211
++ S EL + K I P L+ II K ++ ++++ LG + D
Sbjct: 210 IITSKELLQTKLKDIVSLVPRLRHIITVDGKPPTWSEFPKGVIVHTMAAVQALG--VKAD 267
Query: 212 LLIKRLSNLIPNECCSLIYTSGTTGNPKGVMMSHDNLIWTASCLDYHLKFRSSKEVLISY 391
+ K S +P++ ++YTSG+TG PKGVM+SH N+I + + + + ++V I Y
Sbjct: 268 VDKKAHSKPLPSDIAVIMYTSGSTGIPKGVMISHSNIIASITGMARRIPRLGEEDVYIGY 327
Query: 392 LPLSHIAAQMVDIIVPLICQA---TVYFAQPDAL--------KGTLGNTLREVHPTYFIG 538
LPL+H+ +++ L+C + + ++ P L KG+ G+T + PT
Sbjct: 328 LPLAHV----LELSAELVCLSHGCRIGYSSPQTLADQSSKIKKGSKGDT-SVLKPTLMAA 382
Query: 539 VPRVFEKIMEQMKEMASQLSSLKKYISSWAKDIGYRTTLAKMSGESGPFGYQLAKCILFN 718
VP + ++I + + +++S+ ++ + A + KM S L +F
Sbjct: 383 VPEIMDRIYKNVMNKVNEMSAFQRNLFILAYNY-------KMEQISKGCSTPLCDRFVFR 435
Query: 719 KVHKALGLDRCVAIISSAAPLSHDALVYFGSLDLVIC----EIYGMSECSGPHTVS 874
V + LG ++ APLS + +++ C + YG++E +G T++
Sbjct: 436 NVRRLLG-GNIRVLLCGGAPLSATTQRF---MNICFCCPVGQGYGLTESTGAGTIT 487
>sp|P18163|ACSL1_RAT Long-chain-fatty-acid--CoA ligase 1 (Long-chain acyl-CoA synthetase
1) (LACS 1) (Long-chain-fatty-acid--CoA ligase, liver
isozyme)
Length = 699
Score = 78.2 bits (191), Expect = 3e-14
Identities = 63/220 (28%), Positives = 117/220 (53%), Gaps = 8/220 (3%)
Frame = +2
Query: 242 PNECCSLIYTSGTTGNPKGVMMSHDNLIWTASCLDYHLK---FRSSKEVLISYLPLSHIA 412
P + + +TSGTTGNPKG M++H N++ S + S ++VLIS+LPL+H+
Sbjct: 268 PEDLAIICFTSGTTGNPKGAMVTHQNIMNDCSGFIKATESAFIASPEDVLISFLPLAHMF 327
Query: 413 AQMVDIIVPLICQ-ATVYFAQPDALKGTLGNTLREVHPTYFIGVPRVFEKIMEQMKEMAS 589
+V+ + ++C A + F Q D L + L+ + PT F VPR+ ++ +++ A+
Sbjct: 328 ETVVECV--MLCHGAKIGFFQGDI--RLLMDDLKVLQPTIFPVVPRLLNRMFDRIFGQAN 383
Query: 590 QLSSLKKYISSWA---KDIGYRTTLAKMSGESGPFGYQLAKCILFNKVHKALGLDRCVAI 760
+S+K+++ +A K+ R+ + + + L ++F+K+ +LG + +
Sbjct: 384 --TSVKRWLLDFASKRKEAELRSGIVRNN--------SLWDKLIFHKIQSSLG-GKVRLM 432
Query: 761 ISSAAPLSHDALVYF-GSLDLVICEIYGMSECSGPHTVSL 877
I+ AAP+S L + +L E YG +EC+ +SL
Sbjct: 433 ITGAAPVSATVLTFLRAALGCQFYEGYGQTECTAGCCLSL 472
>sp|Q9CZW4|ACSL3_MOUSE Long-chain-fatty-acid--CoA ligase 3 (Long-chain acyl-CoA synthetase
3) (LACS 3)
Length = 720
Score = 77.8 bits (190), Expect = 4e-14
Identities = 71/296 (23%), Positives = 136/296 (45%), Gaps = 16/296 (5%)
Frame = +2
Query: 35 VVGSSELFK-KYMSIADKCPTLKAIIYYGEKVEESAKNNSKLLIYDWTEFMNLGNDINDD 211
++ S EL + K I P L+ II K ++ ++++ LG N +
Sbjct: 210 IITSKELLQTKLKEIVSLVPRLRHIITVDGKPPTWSEFPKGVIVHTMAAVQALGVKANVE 269
Query: 212 LLIKRLSNLIPNECCSLIYTSGTTGNPKGVMMSHDNLIWTASCLDYHLKFRSSKEVLISY 391
K S +P++ ++YTSG+TG PKGVM+SH N+I + + + + ++V I Y
Sbjct: 270 K--KAHSKPLPSDIAVIMYTSGSTGIPKGVMISHSNIIASITGMARRIPRLGEEDVYIGY 327
Query: 392 LPLSHIAAQMVDIIVPLICQA---TVYFAQPDAL--------KGTLGNTLREVHPTYFIG 538
LPL+H+ +++ L+C + + ++ P L KG+ G+T + PT
Sbjct: 328 LPLAHV----LELSAELVCLSHGCRIGYSSPQTLADQSSKIKKGSKGDT-SVLKPTLMAA 382
Query: 539 VPRVFEKIMEQMKEMASQLSSLKKYISSWAKDIGYRTTLAKMSGESGPFGYQLAKCILFN 718
VP + ++I + + +++S+ ++ + A + KM S L +F
Sbjct: 383 VPEIMDRIYKNVMNKVNEMSAFQRNLFILAYNY-------KMEQISKGCSTPLCDRFVFR 435
Query: 719 KVHKALGLDRCVAIISSAAPLSHDALVYFGSLDLVIC----EIYGMSECSGPHTVS 874
V + LG ++ APLS + +++ C + YG++E +G T++
Sbjct: 436 NVRRLLG-GNIRLLLCGGAPLSATTQRF---MNICFCCPVGQGYGLTESTGAGTIT 487
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 100,807,630
Number of Sequences: 369166
Number of extensions: 2005982
Number of successful extensions: 5347
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5068
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5301
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8982382310
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)