Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_020_B12
(542 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q60648|SAP3_MOUSE Ganglioside GM2 activator precursor (G... 74 2e-13
sp|Q8HXX6|SAP3_MACFA Ganglioside GM2 activator precursor (G... 74 2e-13
sp|P17900|SAP3_HUMAN Ganglioside GM2 activator precursor (G... 73 4e-13
sp|Q58455|Y1055_METJA Hypothetical protein MJ1055 32 1.00
sp|P19730|DMPL_PSEUF Phenol hydroxylase P1 protein (Phenol ... 32 1.3
sp|P25588|MRC1_YEAST Mediator of replication checkpoint pro... 32 1.3
sp|P38353|SSH1_YEAST Sec sixty-one protein homolog 31 1.7
sp|Q8AA76|OXAA_BACTN Inner membrane protein oxaA 30 3.8
sp|Q00689|GP63_LEIGU Leishmanolysin precursor (Cell surface... 29 6.5
sp|O71150|ADEN_ADEB4 Adenain (Endoprotease) (Late L3 23 kDa... 29 8.4
>sp|Q60648|SAP3_MOUSE Ganglioside GM2 activator precursor (GM2-AP) (Cerebroside sulfate
activator protein) (Shingolipid activator protein 3)
(SAP-3)
Length = 193
Score = 74.3 bits (181), Expect = 2e-13
Identities = 43/155 (27%), Positives = 77/155 (49%), Gaps = 1/155 (0%)
Frame = +2
Query: 53 DCGGNSIRVNHFKLSPEKLTFNSFFSVSTNASILQDIEEPVRLKLNIWRKVFGFNVYIPC 232
D G + + + P+ + VS + P +++L + ++V GF V IPC
Sbjct: 40 DEGKDPAVIKSLTIQPDPIVVPGDVVVSLEGKTSVPLTAPQKVELTVEKEVAGFWVKIPC 99
Query: 233 IAKMGSCTYENGCEELEKVI-LGTECPPEILQLGLKCECPLRKFELNLKNQEIYVPKVYG 409
+ ++GSC+YEN C+ +++ I G CP + GL C CP ++ +L VP +
Sbjct: 100 VEQLGSCSYENICDLIDEYIPPGESCPEPLHTYGLPCHCPFKEGTYSLPTSNFTVPDL-- 157
Query: 410 AIAKLLASGKYKISAVLENSKTNYRHMCLEIEAEI 514
+ L++G Y+I ++L S R C++I A +
Sbjct: 158 ELPSWLSTGNYRIQSIL--SSGGKRLGCIKIAASL 190
>sp|Q8HXX6|SAP3_MACFA Ganglioside GM2 activator precursor (GM2-AP) (Cerebroside sulfate
activator protein) (Shingolipid activator protein 3)
(SAP-3)
Length = 190
Score = 73.9 bits (180), Expect = 2e-13
Identities = 47/168 (27%), Positives = 82/168 (48%), Gaps = 1/168 (0%)
Frame = +2
Query: 14 IGFFTVYECVQVKDCGGNSIRVNHFKLSPEKLTFNSFFSVSTNASILQDIEEPVRLKLNI 193
+G F+ C + KD + L P+ + +VS S + P++++L +
Sbjct: 28 LGSFSWDNCDEGKD----PAVIRSLTLEPDPILIPGNVTVSVVGSTSVLLSSPLKVELVL 83
Query: 194 WRKVFGFNVYIPCIAKMGSCTYENGCEELEKVI-LGTECPPEILQLGLKCECPLRKFELN 370
++V G + IPC +GSCT+E+ C+ L+ +I G CP + GL C CP ++ +
Sbjct: 84 EKEVAGLWIKIPCTDYIGSCTFEDFCDVLDMLIPTGEPCPEPLRTYGLPCHCPFKEGTYS 143
Query: 371 LKNQEIYVPKVYGAIAKLLASGKYKISAVLENSKTNYRHMCLEIEAEI 514
L E VP + + L +G Y+I ++L N R C++I A +
Sbjct: 144 LPKSEFVVPHL--ELPSWLTTGNYRIESILSN--RGKRLGCIKIAASL 187
>sp|P17900|SAP3_HUMAN Ganglioside GM2 activator precursor (GM2-AP) (Cerebroside sulfate
activator protein) (Shingolipid activator protein 3)
(SAP-3) [Contains: Ganglioside GM2 activator isoform
short]
Length = 193
Score = 73.2 bits (178), Expect = 4e-13
Identities = 44/155 (28%), Positives = 77/155 (49%), Gaps = 1/155 (0%)
Frame = +2
Query: 53 DCGGNSIRVNHFKLSPEKLTFNSFFSVSTNASILQDIEEPVRLKLNIWRKVFGFNVYIPC 232
D G + + L P+ + ++S S + P+++ L + ++V G + IPC
Sbjct: 40 DEGKDPAVIRSLTLEPDPIVVPGNVTLSVVGSTSVPLSSPLKVDLVLEKEVAGLWIKIPC 99
Query: 233 IAKMGSCTYENGCEELEKVI-LGTECPPEILQLGLKCECPLRKFELNLKNQEIYVPKVYG 409
+GSCT+E+ C+ L+ +I G CP + GL C CP ++ +L E VP +
Sbjct: 100 TDYIGSCTFEHFCDVLDMLIPTGEPCPEPLRTYGLPCHCPFKEGTYSLPKSEFVVPDL-- 157
Query: 410 AIAKLLASGKYKISAVLENSKTNYRHMCLEIEAEI 514
+ L +G Y+I +VL S + R C++I A +
Sbjct: 158 ELPSWLTTGNYRIESVL--SSSGKRLGCIKIAASL 190
>sp|Q58455|Y1055_METJA Hypothetical protein MJ1055
Length = 326
Score = 32.0 bits (71), Expect = 1.00
Identities = 20/77 (25%), Positives = 39/77 (50%)
Frame = -3
Query: 342 SHFKPSCRISGGHSVPKITFSNSSQPFSYVQDPIFAIQGMYTLKPNTFLQIFNFSLTGSS 163
++FK + I G + + N + F+Y+ D + I + +K + +IFN G+S
Sbjct: 197 AYFKFAKNILLGKEIEVYNYGNMERDFTYISDVVDGI--LRAIKKDFDYEIFNL---GNS 251
Query: 162 MSCKILAFVDTEKKELN 112
K++ F++ +K LN
Sbjct: 252 KPVKLMYFIELIEKYLN 268
>sp|P19730|DMPL_PSEUF Phenol hydroxylase P1 protein (Phenol 2-monooxygenase P1 component)
Length = 331
Score = 31.6 bits (70), Expect = 1.3
Identities = 26/98 (26%), Positives = 44/98 (44%), Gaps = 9/98 (9%)
Frame = +2
Query: 158 DIEEPVR--LKLNIWRKV-----FGFNVYIPCIAKMGSCTYENGCEELEKVILGTECPPE 316
++ +P R +++ W KV F + Y+ A+M T E+ EK L + P E
Sbjct: 56 ELHDPTRTAIRMTDWHKVTDPRQFYYGAYVQTRARMQEAT-EHAYGFCEKRELLSRLPAE 114
Query: 317 ILQLGLKCECPLRKFEL--NLKNQEIYVPKVYGAIAKL 424
+ L+C PLR EL N+ N I + + ++
Sbjct: 115 LQAKLLRCLVPLRHAELGANMNNSSIAGDSIAATVTQM 152
>sp|P25588|MRC1_YEAST Mediator of replication checkpoint protein 1 (DNA replication
checkpoint mediator MRC1)
Length = 1096
Score = 31.6 bits (70), Expect = 1.3
Identities = 15/39 (38%), Positives = 24/39 (61%)
Frame = -3
Query: 237 AIQGMYTLKPNTFLQIFNFSLTGSSMSCKILAFVDTEKK 121
AI+ +YTLK N+ ++ F S T S+ S + +D EK+
Sbjct: 946 AIEDLYTLKQNSSIKSFTNSQTDSTTSKTVNTIIDLEKR 984
>sp|P38353|SSH1_YEAST Sec sixty-one protein homolog
Length = 490
Score = 31.2 bits (69), Expect = 1.7
Identities = 19/77 (24%), Positives = 36/77 (46%)
Frame = -3
Query: 300 VPKITFSNSSQPFSYVQDPIFAIQGMYTLKPNTFLQIFNFSLTGSSMSCKILAFVDTEKK 121
+PK T N V DPI+ ++G++ +P T L+ F S + ++LA + K
Sbjct: 53 LPKATTPN-------VNDPIYFLRGVFGCEPRTLLEFGLFPNISSGLILQLLAGLKVIKV 105
Query: 120 ELNVNFSGDNLKWLTRI 70
+ + + LT++
Sbjct: 106 NFKIQSDRELFQSLTKV 122
>sp|Q8AA76|OXAA_BACTN Inner membrane protein oxaA
Length = 618
Score = 30.0 bits (66), Expect = 3.8
Identities = 19/68 (27%), Positives = 30/68 (44%), Gaps = 4/68 (5%)
Frame = -3
Query: 282 SNSSQPFSYVQDPIFAIQGMYTLKPNTFLQIFNFSLTGSSMSCKILAFVDTE----KKEL 115
++SS D I +YTLKPN++L F TG +VD + ++L
Sbjct: 155 TDSSVTMRLAADNASYIDFIYTLKPNSYLMNFEIKATGMEGKLASTEYVDIDWTQRARQL 214
Query: 114 NVNFSGDN 91
F+ +N
Sbjct: 215 EKGFTYEN 222
>sp|Q00689|GP63_LEIGU Leishmanolysin precursor (Cell surface protease) (Major surface
glycoprotein) (Protein gp63) (Promastigote surface
endopeptidase) (Major surface protease)
Length = 621
Score = 29.3 bits (64), Expect = 6.5
Identities = 26/110 (23%), Positives = 47/110 (42%), Gaps = 6/110 (5%)
Frame = -3
Query: 402 TFGTYIS*FFK-FNSNFLRGHSHFKPSCRISGGHSVPKITFSNSSQP-----FSYVQDPI 241
T+ T + +F+ FN FL G+S F C + G+S S+ P FS D
Sbjct: 427 TYSTPMPPYFEYFNDTFLAGYSAFLDYCPFTLGYSNGACNQDPSTAPALLKEFSVFSDAS 486
Query: 240 FAIQGMYTLKPNTFLQIFNFSLTGSSMSCKILAFVDTEKKELNVNFSGDN 91
+ G + +P T ++ ++ +++ C DT + +V G +
Sbjct: 487 RCLDGAF--QPTTAREVLMYNALCANVMC------DTAARTYSVQVRGSS 528
>sp|O71150|ADEN_ADEB4 Adenain (Endoprotease) (Late L3 23 kDa protein)
Length = 201
Score = 28.9 bits (63), Expect = 8.4
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = +3
Query: 231 VSRKWDPVHMKMVVKNWRKLFWERNALQKFYN 326
V+ WDP++ KM + + L W+ + LQ YN
Sbjct: 57 VAMAWDPIYYKMYI--FDPLGWKESQLQSLYN 86
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 65,951,395
Number of Sequences: 369166
Number of extensions: 1369900
Number of successful extensions: 3259
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3185
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3256
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 3734833040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)