Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_020_B08
(787 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P11708|MDHC_PIG Malate dehydrogenase, cytoplasmic 266 5e-71
sp|P40925|MDHC_HUMAN Malate dehydrogenase, cytoplasmic 263 4e-70
sp|Q04820|MDHC_ECHGR Malate dehydrogenase, cytoplasmic 262 9e-70
sp|P14152|MDHC_MOUSE Malate dehydrogenase, cytoplasmic 260 3e-69
sp|P57106|MDHD_ARATH Malate dehydrogenase, cytoplasmic 2 230 3e-60
sp|P93819|MDHC1_ARATH Malate dehydrogenase, cytoplasmic 1 230 4e-60
sp|O48905|MDHC_MEDSA Malate dehydrogenase, cytoplasmic 227 3e-59
sp|O24047|MDHC_MESCR Malate dehydrogenase, cytoplasmic 226 4e-59
sp|Q9SML8|MDHC_BETVU Malate dehydrogenase, cytoplasmic 223 4e-58
sp|Q08062|MDHC_MAIZE Malate dehydrogenase, cytoplasmic 223 5e-58
>sp|P11708|MDHC_PIG Malate dehydrogenase, cytoplasmic
Length = 334
Score = 266 bits (680), Expect = 5e-71
Identities = 143/232 (61%), Positives = 166/232 (71%), Gaps = 3/232 (1%)
Frame = +3
Query: 3 LKANVNIFKVQGEALDKYAKKTVRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHN 182
LKANV IFK QG ALDKYAKK+V+V VVGNPANTNCL SK APSIPKENFS LTRLDHN
Sbjct: 102 LKANVKIFKCQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHN 161
Query: 183 RAIAQIAGKAGVPCDHVRRCIIWGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLK 362
RA AQIA K GV D V+ IIWGNHS+TQYPD +H VK KEV V +AVKD++WLK
Sbjct: 162 RAKAQIALKLGVTSDDVKNVIIWGNHSSTQYPDVNHAKVK-LQAKEVGVYEAVKDDSWLK 220
Query: 363 NDFLKTVQTRGAAVIXXXXXXXXXXXXXXXXDHCRDWFHGTK--EWVSMAVYSDG-SYGV 533
+F+ TVQ RGAAVI DH RD + GT E+VSM + SDG SYGV
Sbjct: 221 GEFITTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGIISDGNSYGV 280
Query: 534 PKDVIFSFPVTIEHGKWHIVQGLTIDDFSRGKLELTGKELIEERDQALAFCS 689
P D+++SFPVTI+ W IV+GL I+DFSR K++LT KEL EE++ A F S
Sbjct: 281 PDDLLYSFPVTIKDKTWKIVEGLPINDFSREKMDLTAKELAEEKETAFEFLS 332
>sp|P40925|MDHC_HUMAN Malate dehydrogenase, cytoplasmic
Length = 334
Score = 263 bits (672), Expect = 4e-70
Identities = 141/232 (60%), Positives = 166/232 (71%), Gaps = 3/232 (1%)
Frame = +3
Query: 3 LKANVNIFKVQGEALDKYAKKTVRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHN 182
LKANV IFK QG ALDKYAKK+V+V VVGNPANTNCL SK APSIPKENFS LTRLDHN
Sbjct: 102 LKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHN 161
Query: 183 RAIAQIAGKAGVPCDHVRRCIIWGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLK 362
RA AQIA K GV + V+ IIWGNHS+TQYPD +H VK GKEV V +A+KD++WLK
Sbjct: 162 RAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVK-LQGKEVGVYEALKDDSWLK 220
Query: 363 NDFLKTVQTRGAAVIXXXXXXXXXXXXXXXXDHCRDWFHGTK--EWVSMAVYSDG-SYGV 533
+F+ TVQ RGAAVI DH RD + GT E+VSM V SDG SYGV
Sbjct: 221 GEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGV 280
Query: 534 PKDVIFSFPVTIEHGKWHIVQGLTIDDFSRGKLELTGKELIEERDQALAFCS 689
P D+++SFPV I++ W V+GL I+DFSR K++LT KEL EE++ A F S
Sbjct: 281 PDDLLYSFPVVIKNKTWKFVEGLPINDFSREKMDLTAKELTEEKESAFEFLS 332
>sp|Q04820|MDHC_ECHGR Malate dehydrogenase, cytoplasmic
Length = 332
Score = 262 bits (669), Expect = 9e-70
Identities = 135/229 (58%), Positives = 161/229 (70%), Gaps = 3/229 (1%)
Frame = +3
Query: 3 LKANVNIFKVQGEALDKYAKKTVRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHN 182
L +NV IFK QGEALDKYAKKTV+V VVGNPANTNCLIMSKYAPSIPKENF+ALTRLDHN
Sbjct: 102 LSSNVKIFKEQGEALDKYAKKTVKVLVVGNPANTNCLIMSKYAPSIPKENFTALTRLDHN 161
Query: 183 RAIAQIAGKAGVPCDHVRRCIIWGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLK 362
RAI Q+A KAGVP V+ IWGNHSN Q+PD H +V DGK+ + + DE W+K
Sbjct: 162 RAIYQVAAKAGVPNTCVKNVCIWGNHSNKQFPDLSHAVVT-KDGKQHPAKELINDEKWVK 220
Query: 363 NDFLKTVQTRGAAVIXXXXXXXXXXXXXXXXDHCRDWFHGTK--EWVSMAVYSDGS-YGV 533
F+ VQ RGAAVI D RDW+ GTK EWVSM+VYS G YG
Sbjct: 221 EVFIPCVQNRGAAVIGLRKLSRAASAAKAIVDQMRDWWFGTKEGEWVSMSVYSTGDHYGA 280
Query: 534 PKDVIFSFPVTIEHGKWHIVQGLTIDDFSRGKLELTGKELIEERDQALA 680
PKD+ FSFPVTI+ G + +V GL++D++SR L+ EL++ER+ ALA
Sbjct: 281 PKDIYFSFPVTIKDGHYKVVDGLSMDEWSRSLFNLSADELVDEREVALA 329
>sp|P14152|MDHC_MOUSE Malate dehydrogenase, cytoplasmic
Length = 334
Score = 260 bits (664), Expect = 3e-69
Identities = 139/232 (59%), Positives = 165/232 (71%), Gaps = 3/232 (1%)
Frame = +3
Query: 3 LKANVNIFKVQGEALDKYAKKTVRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHN 182
LKANV IFK QG AL+KYAKK+V+V VVGNPANTNCL SK APSIPKENFS LTRLDHN
Sbjct: 102 LKANVKIFKSQGTALEKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHN 161
Query: 183 RAIAQIAGKAGVPCDHVRRCIIWGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLK 362
RA +QIA K GV D V+ IIWGNHS+TQYPD +H VK GKEV V +A+KD++WLK
Sbjct: 162 RAKSQIALKLGVTADDVKNVIIWGNHSSTQYPDVNHAKVK-LQGKEVGVYEALKDDSWLK 220
Query: 363 NDFLKTVQTRGAAVIXXXXXXXXXXXXXXXXDHCRDWFHGTK--EWVSMAVYSDG-SYGV 533
+F+ TVQ RGAAVI DH RD + GT E+VSM V SDG SYGV
Sbjct: 221 GEFITTVQQRGAAVIKARKLSSAMSAAKAIADHIRDIWFGTPEGEFVSMGVISDGNSYGV 280
Query: 534 PKDVIFSFPVTIEHGKWHIVQGLTIDDFSRGKLELTGKELIEERDQALAFCS 689
P D+++S PV I++ W V+GL I+DFSR K++LT KEL EE++ A F S
Sbjct: 281 PDDLLYSLPVVIKNKTWKFVEGLPINDFSREKMDLTAKELTEEKETAFEFLS 332
>sp|P57106|MDHD_ARATH Malate dehydrogenase, cytoplasmic 2
Length = 332
Score = 230 bits (587), Expect = 3e-60
Identities = 118/231 (51%), Positives = 153/231 (66%), Gaps = 2/231 (0%)
Frame = +3
Query: 3 LKANVNIFKVQGEALDKYAKKTVRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHN 182
+ NV+I+K Q AL+K+A +V VV NPANTN LI+ ++APSIP++N + LTRLDHN
Sbjct: 103 MSKNVSIYKSQASALEKHAAPNCKVLVVANPANTNALILKEFAPSIPEKNITCLTRLDHN 162
Query: 183 RAIAQIAGKAGVPCDHVRRCIIWGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLK 362
RA+ Q++ + VP V+ IIWGNHS+TQYPD +H VK G E V + VK++ WL
Sbjct: 163 RALGQVSERLSVPVSDVKNVIIWGNHSSTQYPDVNHATVKTSVG-EKPVRELVKNDEWLN 221
Query: 363 NDFLKTVQTRGAAVIXXXXXXXXXXXXXXXXDHCRDWFHGTKE--WVSMAVYSDGSYGVP 536
+F+ TVQ RGAA+I DH RDW GT E +VSM VYSDGSY VP
Sbjct: 222 GEFISTVQQRGAAIIKARKLSSALSAASSACDHIRDWVVGTPEGTFVSMGVYSDGSYNVP 281
Query: 537 KDVIFSFPVTIEHGKWHIVQGLTIDDFSRGKLELTGKELIEERDQALAFCS 689
+I+SFPVT +G+W IVQGL IDD SR K++LT +EL EE+D A + S
Sbjct: 282 AGLIYSFPVTCRNGEWTIVQGLPIDDASRKKMDLTAEELKEEKDLAYSCLS 332
>sp|P93819|MDHC1_ARATH Malate dehydrogenase, cytoplasmic 1
Length = 332
Score = 230 bits (586), Expect = 4e-60
Identities = 118/231 (51%), Positives = 152/231 (65%), Gaps = 2/231 (0%)
Frame = +3
Query: 3 LKANVNIFKVQGEALDKYAKKTVRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHN 182
+ NV+I+K Q AL+K+A +V VV NPANTN LI+ ++APSIP++N S LTRLDHN
Sbjct: 103 MSKNVSIYKSQAAALEKHAAPNCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHN 162
Query: 183 RAIAQIAGKAGVPCDHVRRCIIWGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLK 362
RA+ QI+ + VP V+ IIWGNHS++QYPD +H V+ G E V + VKD+ WL
Sbjct: 163 RALGQISERLSVPVSDVKNVIIWGNHSSSQYPDVNHAKVQTSSG-EKPVRELVKDDAWLD 221
Query: 363 NDFLKTVQTRGAAVIXXXXXXXXXXXXXXXXDHCRDWFHGTKE--WVSMAVYSDGSYGVP 536
+F+ TVQ RGAA+I DH RDW GT E +VSM VYSDGSY VP
Sbjct: 222 GEFISTVQQRGAAIIKARKLSSALSAASSACDHIRDWVLGTPEGTFVSMGVYSDGSYSVP 281
Query: 537 KDVIFSFPVTIEHGKWHIVQGLTIDDFSRGKLELTGKELIEERDQALAFCS 689
+I+SFPVT +G W IVQGL ID+ SR K++LT +EL EE+D A + S
Sbjct: 282 SGLIYSFPVTCRNGDWSIVQGLPIDEVSRKKMDLTAEELKEEKDLAYSCLS 332
>sp|O48905|MDHC_MEDSA Malate dehydrogenase, cytoplasmic
Length = 332
Score = 227 bits (578), Expect = 3e-59
Identities = 116/226 (51%), Positives = 148/226 (65%), Gaps = 2/226 (0%)
Frame = +3
Query: 3 LKANVNIFKVQGEALDKYAKKTVRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHN 182
+ NV+I+K Q AL+K+A +V VV NPANTN LI+ ++APSIP+ N S LTRLDHN
Sbjct: 103 MSKNVSIYKSQASALEKHAAANCKVLVVANPANTNALILKEFAPSIPERNISCLTRLDHN 162
Query: 183 RAIAQIAGKAGVPCDHVRRCIIWGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLK 362
RA+ QI+ + V V+ IIWGNHS+TQYPD +H V G E V V D+ WL
Sbjct: 163 RALGQISERLNVQVSDVKNVIIWGNHSSTQYPDVNHATVNTPAG-EKPVRQLVSDDAWLN 221
Query: 363 NDFLKTVQTRGAAVIXXXXXXXXXXXXXXXXDHCRDWFHGTKE--WVSMAVYSDGSYGVP 536
+F+ TVQ RGAA+I DH RDW GT + +VSM VYSDGSY VP
Sbjct: 222 GEFISTVQQRGAAIIKARKLSSALSAASAACDHIRDWVLGTPQGTFVSMGVYSDGSYNVP 281
Query: 537 KDVIFSFPVTIEHGKWHIVQGLTIDDFSRGKLELTGKELIEERDQA 674
+I+SFPVT +G+W IVQGL+ID+FSR KL+LT +EL EE++ A
Sbjct: 282 SGLIYSFPVTCANGEWKIVQGLSIDEFSRKKLDLTAEELTEEKNLA 327
>sp|O24047|MDHC_MESCR Malate dehydrogenase, cytoplasmic
Length = 332
Score = 226 bits (577), Expect = 4e-59
Identities = 114/226 (50%), Positives = 147/226 (65%), Gaps = 2/226 (0%)
Frame = +3
Query: 3 LKANVNIFKVQGEALDKYAKKTVRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHN 182
+ NV+I+K Q AL+++A +V VV NPANTN LI+ ++APSIP++N S LTRLDHN
Sbjct: 103 MSKNVSIYKAQASALEQHAAPNCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHN 162
Query: 183 RAIAQIAGKAGVPCDHVRRCIIWGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLK 362
RA+ QI+ + V V+ IIWGNHS+TQYPD +H VK G + V + V D+ WL
Sbjct: 163 RALGQISERLNVQVSDVKNVIIWGNHSSTQYPDVNHATVK-TQGVDKPVRELVADDAWLN 221
Query: 363 NDFLKTVQTRGAAVIXXXXXXXXXXXXXXXXDHCRDWFHGTKE--WVSMAVYSDGSYGVP 536
+F+ TVQ RGAA+I DH DW GT E WVSM VYSDGSY VP
Sbjct: 222 GEFITTVQQRGAAIIKARKLSSALSAASSACDHIHDWVLGTPEGTWVSMGVYSDGSYNVP 281
Query: 537 KDVIFSFPVTIEHGKWHIVQGLTIDDFSRGKLELTGKELIEERDQA 674
+I+SFPVT ++G+W IVQGL IDD SR K++ T EL+EE+ A
Sbjct: 282 AGIIYSFPVTCKNGEWTIVQGLPIDDDSRKKMDATAAELVEEKTLA 327
>sp|Q9SML8|MDHC_BETVU Malate dehydrogenase, cytoplasmic
Length = 332
Score = 223 bits (569), Expect = 4e-58
Identities = 115/226 (50%), Positives = 149/226 (65%), Gaps = 2/226 (0%)
Frame = +3
Query: 12 NVNIFKVQGEALDKYAKKTVRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAI 191
NV+I+K Q AL++YA +V VV NPANTN LI+ ++APSIP++N + LTRLDHNRA+
Sbjct: 106 NVSIYKSQASALEQYAAPNCKVLVVANPANTNALILKEFAPSIPEKNITCLTRLDHNRAL 165
Query: 192 AQIAGKAGVPCDHVRRCIIWGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLKNDF 371
QI+ + V+ IIWGNHS++QYPD + VK G E +V + V D+ WL +F
Sbjct: 166 GQISERLNAQVSDVKNVIIWGNHSSSQYPDVNPCTVKTGSG-EKAVRELVADDAWLNGEF 224
Query: 372 LKTVQTRGAAVIXXXXXXXXXXXXXXXXDHCRDWFHGTKE--WVSMAVYSDGSYGVPKDV 545
+ TVQ RGAA+I DH RDW GT E WVSM VYSDGSY VP +
Sbjct: 225 ITTVQQRGAAIIKARKLSSALSAASSACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGI 284
Query: 546 IFSFPVTIEHGKWHIVQGLTIDDFSRGKLELTGKELIEERDQALAF 683
I+SFPVT + G+W IVQGL ID+ SR K++ TG EL+EE+ ALA+
Sbjct: 285 IYSFPVTCKDGEWKIVQGLPIDEVSRQKMDATGAELVEEK--ALAY 328
>sp|Q08062|MDHC_MAIZE Malate dehydrogenase, cytoplasmic
Length = 332
Score = 223 bits (568), Expect = 5e-58
Identities = 114/226 (50%), Positives = 147/226 (65%), Gaps = 2/226 (0%)
Frame = +3
Query: 3 LKANVNIFKVQGEALDKYAKKTVRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHN 182
+ NV+I+K Q AL+ +A +V VV NPANTN LI+ ++APSIP++N + LTRLDHN
Sbjct: 103 MSKNVSIYKSQASALEAHAAPNCKVLVVANPANTNALILKEFAPSIPEKNVTCLTRLDHN 162
Query: 183 RAIAQIAGKAGVPCDHVRRCIIWGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLK 362
RA+ QI+ + V V+ IIWGNHS++QYPD +H VK G E V + V D+ WL
Sbjct: 163 RALGQISERLNVQVSDVKNVIIWGNHSSSQYPDVNHATVKTSTG-EKPVRELVSDDEWLN 221
Query: 363 NDFLKTVQTRGAAVIXXXXXXXXXXXXXXXXDHCRDWFHGTKE--WVSMAVYSDGSYGVP 536
+F+ TVQ RGAA+I DH RDW GT E +VSM VYSDGSYGVP
Sbjct: 222 GEFITTVQQRGAAIIKARKFSSALSAASSACDHIRDWVLGTPEGTFVSMGVYSDGSYGVP 281
Query: 537 KDVIFSFPVTIEHGKWHIVQGLTIDDFSRGKLELTGKELIEERDQA 674
+I+SFPVT G+W IVQGL ID+FSR K++ T +EL EE+ A
Sbjct: 282 SGLIYSFPVTCSGGEWKIVQGLPIDEFSRKKMDATAQELTEEKTLA 327
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 86,990,057
Number of Sequences: 369166
Number of extensions: 1772215
Number of successful extensions: 4508
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4281
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4381
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7405750800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)