Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Dr_sW_020_A23 (345 letters) Database: Non-redundant SwissProt sequences 184,735 sequences; 68,354,980 total letters Score E Sequences producing significant alignments: (bits) Value sp|P19751|R1AB_CVMJH Replicase polyprotein 1ab (pp1ab) (ORF... 29 2.9 sp|P16342|R1AB_CVMA5 Replicase polyprotein 1ab (pp1ab) (ORF... 29 2.9 sp|Q9PYA3|R1AB_CVM2 Replicase polyprotein 1ab (pp1ab) (ORF1... 29 2.9 sp|P56934|LEU2_BUCAI 3-isopropylmalate dehydratase large su... 29 2.9 sp|P87110|ITR2_SCHPO Myo-inositol transporter 2 28 6.6 sp|P23266|O1361_RAT Olfactory receptor 1361 (Olfactory rece... 28 8.6
>sp|P19751|R1AB_CVMJH Replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) [Includes: Replicase polyprotein 1a (pp1a) (ORF1a)] [Contains: p28; p65; p210 (Papain-like proteinases 1/2) (PL1-PRO/PL2-PRO); Peptide HD2 (p44); 3C-like proteinase (3CL-PRO) (3CLp) (M-PRO) (p27); Unknown protein 1; p10; p22; p12; Growth factor-like peptide (GFL) (p15); RNA-directed RNA polymerase (RdRp) (Pol) (p100); Helicase (Hel) (p67); Unknown protein 2; p35; Unknown protein 3] Length = 7180 Score = 29.3 bits (64), Expect = 2.9 Identities = 22/74 (29%), Positives = 33/74 (44%), Gaps = 5/74 (6%) Frame = -3 Query: 307 IYIIRLLMLF*QDYQKTLLCLHRSIVFHH--YFHNSLQILFPLFWFLHISFLNHLDF--- 143 ++ I LMLF TL CL+ F+ YF + + ++ L W + + L F Sbjct: 3160 VWCINFLMLFVFQVYPTLSCLYACFYFYTTLYFPSEISVVMHLQWLVMYGAIMPLWFCIT 3219 Query: 142 YSAISFSNCHTCLF 101 Y A+ SN LF Sbjct: 3220 YVAVVVSNHALWLF 3233
>sp|P16342|R1AB_CVMA5 Replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) [Includes: Replicase polyprotein 1a (pp1a) (ORF1a)] [Contains: p28; p65; p210 (Papain-like proteinases 1/2) (PL1-PRO/PL2-PRO); Peptide HD2 (p44); 3C-like proteinase (3CL-PRO) (3CLp) (M-PRO) (p27); Unknown protein 1; p10; p22; p12; Growth factor-like peptide (GFL) (p15); RNA-directed RNA polymerase (RdRp) (Pol) (p100); Helicase (Hel) (p67); Unknown protein 2; p35; Unknown protein 3] Length = 7176 Score = 29.3 bits (64), Expect = 2.9 Identities = 22/74 (29%), Positives = 33/74 (44%), Gaps = 5/74 (6%) Frame = -3 Query: 307 IYIIRLLMLF*QDYQKTLLCLHRSIVFHH--YFHNSLQILFPLFWFLHISFLNHLDF--- 143 ++ I LMLF TL CL+ F+ YF + + ++ L W + + L F Sbjct: 3157 VWCINFLMLFVFQVYPTLSCLYACFYFYTTLYFPSEISVVMHLQWLVMYGAIMPLWFCII 3216 Query: 142 YSAISFSNCHTCLF 101 Y A+ SN LF Sbjct: 3217 YVAVVVSNHALWLF 3230
>sp|Q9PYA3|R1AB_CVM2 Replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) [Includes: Replicase polyprotein 1a (pp1a) (ORF1a)] [Contains: p28; p65; p210 (Papain-like proteinases 1/2) (PL1-PRO/PL2-PRO); Peptide HD2 (p44); 3C-like proteinase (3CL-PRO) (3CLp) (M-PRO) (p27); Unknown protein 1; p10; p22; p12; Growth factor-like peptide (GFL) (p15); RNA-directed RNA polymerase (RdRp) (Pol) (p100); Helicase (Hel) (p67); Unknown protein 2; p35; Unknown protein 3] Length = 7124 Score = 29.3 bits (64), Expect = 2.9 Identities = 22/74 (29%), Positives = 33/74 (44%), Gaps = 5/74 (6%) Frame = -3 Query: 307 IYIIRLLMLF*QDYQKTLLCLHRSIVFHH--YFHNSLQILFPLFWFLHISFLNHLDF--- 143 ++ I LMLF TL CL+ F+ YF + + ++ L W + + L F Sbjct: 3103 VWCINFLMLFVFQVYPTLSCLYACFYFYTTLYFPSEISVVMHLQWLVMYGAIMPLWFCII 3162 Query: 142 YSAISFSNCHTCLF 101 Y A+ SN LF Sbjct: 3163 YVAVVVSNHALWLF 3176
>sp|P56934|LEU2_BUCAI 3-isopropylmalate dehydratase large subunit (Isopropylmalate isomerase) (Alpha-IPM isomerase) (IPMI) Length = 471 Score = 29.3 bits (64), Expect = 2.9 Identities = 17/44 (38%), Positives = 28/44 (63%), Gaps = 2/44 (4%) Frame = +1 Query: 217 NNDERQLNDEDIAEF--FDNLVKRALANE*CKFSIGLKLLKFIS 342 N D+ D+ I +F FDNL+K+ LA CK+ +GLK+ +++ Sbjct: 293 NPDQVISIDQKIPDFSSFDNLIKKDLAKSACKY-MGLKIGTYLT 335
>sp|P87110|ITR2_SCHPO Myo-inositol transporter 2 Length = 557 Score = 28.1 bits (61), Expect = 6.6 Identities = 17/50 (34%), Positives = 24/50 (48%), Gaps = 13/50 (26%) Frame = -3 Query: 229 FHHYFHNSLQILFP-------------LFWFLHISFLNHLDFYSAISFSN 119 F H+FH SL++LF L WF S N + ++SAI F + Sbjct: 313 FQHFFH-SLKVLFTVPSNRRSLFIGCFLQWFQQFSGTNAIQYFSAIIFQS 361
>sp|P23266|O1361_RAT Olfactory receptor 1361 (Olfactory receptor-like protein F5) Length = 313 Score = 27.7 bits (60), Expect = 8.6 Identities = 31/102 (30%), Positives = 45/102 (44%), Gaps = 25/102 (24%) Frame = -3 Query: 319 NQSKIYIIRLLMLF*QDYQKTLLCLHRSIVFHHYFHNSLQILF----------PLFWFL- 173 NQS + LL L Q Q+ LL L I++ +L I+ P+++FL Sbjct: 5 NQSSVTEFLLLGLSRQPQQQQLLFLLFLIMYLATVLGNLLIILAIGTDSRLHTPMYFFLS 64 Query: 172 HISFL--------------NHLDFYSAISFSNCHTCLFLLLV 89 ++SF+ NH+ AISFS C T L+ L V Sbjct: 65 NLSFVDVCFSSTTVPKVLANHILGSQAISFSGCLTQLYFLAV 106
Database: Non-redundant SwissProt sequences Posted date: Dec 6, 2005 7:40 AM Number of letters in database: 68,354,980 Number of sequences in database: 184,735 Database: swissprot.01 Posted date: Dec 6, 2005 8:18 AM Number of letters in database: 66,202,850 Number of sequences in database: 184,431 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 28,850,118 Number of Sequences: 369166 Number of extensions: 431594 Number of successful extensions: 1138 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 1118 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1132 length of database: 68,354,980 effective HSP length: 82 effective length of database: 53,206,710 effective search space used: 1702614720 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)